Microbiota do colo uterino por Gram em pacientes com infeção por papilomavírus humano e outras infecções sexualmente transmissíveis

2021 ◽  
Author(s):  
Marina Mara Sousa de Oliveira ◽  
Hyan Staytskowy Magalhães Martins ◽  
Rafael Pereira de Vasconcelos ◽  
Renata Mirian Nunes Eleutério ◽  
José Eleutério Júnior
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium

Introdução: A microbiota vaginal é um complexo sistema com diversidade de microrganismos. A disbiose parece aumentar o risco de infecções, principalmente as sexualmente transmissíveis, entre as quais por papilomavírus humano, agente associado a lesões cervicais. Objetivo: Avaliar os diferentes tipos de microbiota cervical e as suas características no esfregaço de material residual de citologia em meio líquido, associando com o papilomavírus humano e com Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Trichomonas vaginalis. Métodos: O estudo analisou 179 casos que tinham material residual de citologia em meio líquido. Alíquota do material foi colocado em lâmina adequada, fixado a seco e corado por método de Gram para leitura em microscópio óptico. Outra alíquota foi utilizada para estudo em reação em cadeia da polimerase - transcriptase reversa e multiplex para pesquisas dos microorganismos associados a infecções sexualmente transmissíveis. O teste exato de Fisher com intervalo de confiança foi utilizado para significância estatística. O projeto foi aprovado em comitê de ética sob número 24071519.9.0000.5049 (UniChristus). Resultados: Os casos foram divididos conforme o escore de Nugent aplicado a esfregaços corados pelo método de Gram. Em microbiota cervical normal (escores de 0 a 3), 100 casos (55,86%); em microbiota intermediária (escore de 4 a 6), 51 casos (28,5%); em sugestivo de disbiose (escore de 7 a 10), 28 casos (15,64%). Nos casos de disbiose, foram observados: Chlamydia trachomatis (1[3,57%]), Mycoplasma hominis (7[25%]), Ureaplasma urealyticum (1[3,57%]), papilomavírus humano 16/45 (1[3,57%]), papilomavírus humano de alto risco (AR) (3[10,71%]) e AR e 16/45 (1[3,57%]). No grupo normal, foi a seguinte distribuição: Ureaplasma urealyticum (1[1%]), papilomavírus humano 16 (2[2%]), papilomavírus humano 18/45 (3[3%]), AR (13[13%]). No grupo intermediário, a distribuição foi: Ureaplasma urealyticum (2[3,9%]), papilomavírus humano AR (5[9,8%]) e papilomavírus humano AR, 16 (1[3,9%]). A única diferença significativa foi de Mycoplasma hominis na disbiose (p<0,0001). Conclusão: O estudo não evidenciou uma associação maior no grupo de disbiose com a maioria das infecções sexualmente transmissíveis, no entanto, com Mycoplasma hominis, foi significativo.

scholarly journals Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fabiana Pirani Carneiro ◽  
Andersen Charles Darós ◽  
Adriana Cysneiro Milhomem Darós ◽  
Tércia Maria Mendes Lousa de Castro ◽  
Marcos de Vasconcelos Carneiro ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Multiplex Pcr ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Diagnostic Methods ◽  
Pap Smears ◽  
Ureaplasma Parvum

Introduction. Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum detected by multiplex PCR. Methods. Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit. Results. Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0). Multiple pathogens were observed in 12.8% of samples. Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%. Conclusion. Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, or Trichomonas vaginalis. A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.

scholarly journals INCIDENCE OF SEXUALLY TRANSMITTED INFECTIONS: LOCAL STUDY IN UKRAINE

World Science ◽  
2018 ◽  
Vol 2 (8(36)) ◽  
pp. 4-7
Author(s):  
Fedorych P. V. ◽  
Mavrov G. I.
Keyword(s):  
Human Papillomavirus ◽  
Chlamydia Trachomatis ◽  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Local Study ◽  
Sexually Transmitted ◽  
Clinically Significant

Introduction.The structure of incidence of sexually transmitted infections is changing constantly. Information on such changes supports correct planning of clinical and diagnostic activities of institutions providing specialized medical care by qualified specialists.Objective:to investigate the prevalence of sexually transmitted infections with pathogens clinically significant to the genitourinary system in Ukraine and at the local level.Materials and methods. Polymerase chain reaction was used to test the biological material obtained from the genitourinary clinical specimens from subjects with sexually transmitted infections, who underwent clinical and laboratory examinations in Oleksandrivsk Clinical Hospital (Kyiv, Ukraine) for Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Trichomonas vaginalis, Human papillomavirus, and Neisseria gonorrhoea. During 2017, 607 subjects of both genders, including 295 (48.6%) females and 312 (51.4%) males, were examined. Their mean age was 32±3.5.Findings. Chlamydia trachomatis was found in 159 (26.2%) of 607 examined subjects – 85 males and 74 females. Mycoplasma hominis was found in 122 of 585 (21.1%) examined subjects – 64 males and 58 females. Mycoplasma genitalium, respectively, in 17 (6.62%) of 258 subjects – 6 males and 11 females. Ureaplasma urealyticum was found in the largest number of subjects (305, i.e. in 48.77% of 601 examined subjects) – 157 males and 148 females. Trichomonas vaginalis was found in 28 (5.23%) of 535 subjects – 15 males and 13 females. Human papillomavirus was found in 158 of 297 (53.2%) examined subjects – 88 males and 70 females. Neisseria gonorrhea was found in 33 of 297 (8.45%) subjects – 8 males and 25 females.Conclusions. As suggested by the local study of the sexually transmitted infections incidence in Ukraine, the most clinically significant for the genitourinary system are Human papillomavirus (53.2%), Ureaplasma urealyticum (48.77%), Chlamydia trachomatis (26.2%) and Mycoplasma hominis (21.1%). Therefore, tests for these pathogens in the specified region is currently the most appropriate during diagnostic examinations and counselling of subjects with genitourinary infections.

scholarly journals Prevalence Rates of Chlamydia Trachomatis and Other Sexually Transmitted Organisms in Infertile Couples Attending a Tertiary Medical Centre in Malaysia

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Umi Kalsom A ◽  
Suvra B ◽  
Zainul RMR ◽  
Siti Norlia O ◽  
Zalina I ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Married Couples ◽  
Prevalence Rates ◽  
Sexually Transmitted ◽  
Neisseria Gonorrhoea ◽  
Infertile Couples

 INTRODUCTION: Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV) and Mycoplasma genitalium (MG) infections are well recognized and prevalent sexually transmitted infections (STIs). The role of Mycoplasma spp and Ureaplasma spp are still controversial as some are commensals of genitourinary tract. OBJECTIVES: To estimate the prevalence rate of 7 organisms: Chlamydia trachomatis, Neisseria gonorrhoea, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum (UU) and Ureaplasma parvum (UP) in infertile married couples during infertility evaluation. MATERIALS & METHODS: A total of 274 samples from all of the 137 couples who attended the reproductive center from June to December 2014 were collected. Detection of the organisms was performed using multiplex polymerase chain reaction. RESULTS: STI-associated organisms were detected in 35.4% (97/274) of subjects. The prevalence rates of CT, MG, TV, UU, MH were 7.3%,1.1%, 0.4%,5.4% and 5.1% respectively. Twenty-one (7.7%) subjects were positive for more than one organism. 24/274 (8.8%) of subjects had history of urogenital tract-related symptoms and 50% (12/24) were tested positive to one or more organisms. The presence of symptoms in both male and female subjects were found to be 10% (2/20) in CT infection, 10% (7/67) in UP, 14% (2/14) in MH and 13% (2/15) in UU infections. CONCLUSION: Sexually transmitted organisms were detected in one third of subjects planning for fertility evaluation. The absence of symptom in most subjects particularly in CT infection emphasizes the need for microbiological screening during infertility evaluation. The presence of genital ureaplasmas and mycoplasmas in infertile couples should not be neglected. There is a growing need to clarify whether their roles are simply colonizers or pathogens implicated in infertility.

Negonorėjiniai uretritai: etiopatogenezė, klinikos, diagnostikos ir gydymo savitumai

2021 ◽  
Vol 25 (4) ◽  
pp. 259-264
Author(s):  
Simona Žilinskienė ◽  
Arūnas Petkevičius
Keyword(s):  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Gardnerella Vaginalis ◽  
Ureaplasma Parvum

Negonorėjinis uretritas (NGU) yra dažniausia vyrų lytinių takų liga. Mokslinių tyrimų rezultatais pagrįsta, kad pagrindiniai sukėlėjai yra Chlamydia trachomatis, Mycoplasma genitalium, Ureaplasma urealyticum. Įdiegus pažangius molekulinės diagnostikos metodus, dažnai šlaplės mikrofloroje randama Mycoplasma hominis, Ureaplasma parvum, Gardnerella vaginalis ir kitų saprofitinių mikroorganizmų, kurių svarba uretritų etiopatogenezėje yra prieštaringa ir iki galo neišaiškinta. Negydytas vyrų uretritas gali sukelti sutrikimų, susijusių su reprodukcine bei lytine funkcija, ir yra viena iš pagrindinių nevaisingumo priežasčių. Šio straipsnio tikslas yra, apžvelgus mokslinę literatūrą, išanalizuoti vyrų NGU epidemiologiją, priežastis, diagnostikos ir gydymo galimybes.

scholarly journals Molecular epidemiology of non-viral sexually transmitted infections in the central Alpine province of Bolzano, northern Italy from April 2016 to March 2017

2018 ◽  
Vol 33 (1) ◽  
Author(s):  
Richard Aschbacher ◽  
Francesca Romagnoli ◽  
Elisa Masi ◽  
Valentina Pasquetto ◽  
Franco Perino ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Northern Italy ◽  
Sexually Transmitted ◽  
Ureaplasma Parvum ◽  
One Year ◽  
The One

Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium are established or presumed as (??) STI pathogens. The present study aims  at ng describing the one-year molecular epidemiology of these seven pathogens in the Province of Bolzano, Northern Italy. From April 2016 to March 2017, a total of  2,949 patients, mainly females, were enrolled and 3,427 urine, vaginal, endocervical and/or urethral samples were subjected to simultaneous analysis of the seven pathogens by means of Real Time Polymerase Chain Reaction (AnyplexTM II STI-7 Detection Kit Seegene, Seoul, Korea). At least one of the seven microorganisms was detected in 40.7% of patients, with an uneven distribution: 43.1% in females (F) and 29.8% (p<0.001) in males (M). The prevalence of microorganisms was as follows: 30.3% U. parvum (F: 35.6%, M: 8.3%), 6.9% U. urealyticum (F: 6.8%, M: 7.0%), 4.9% M. hominis (F: 5.4%, M: 2.3%), 4.9% C. trachomatis (F: 3.4%, M: 11.4%), 1.1% M. genitalium (F: 1.0%, M: 1.2%), 1.2% N. gonorrhoeae (F: 0.17%, M: 5.6%) and 0.40% T. vaginalis (F: 0.38%, M: 0.53%). Mixed infections were detected in 7.4% of patients. The highest prevalence was observed for U. parvum, followed by U. urealyticum and M. hominis and a significant  presence of multi-pathogen infections was registered.

Chlamydia trachomatis : probable cause of prostatitis

1998 ◽  
Vol 9 (6) ◽  
pp. 350-353 ◽  
Author(s):  
Iwona Ostaszewska ◽  
Bozena Zdrodowska-Stefanow ◽  
Jerzy Badyda ◽  
Katarzyna Pucilo ◽  
Jadwiga Trybula ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Gardnerella Vaginalis ◽  
Igg Antibodies ◽  
Gram Positive Bacteria ◽  
Group I ◽  
Causative Agents ◽  
Group Ii

Summary: Seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. Investigations for the presence of Chlamydia trachomatis in urethral swabs were carried out. The expressed prostatic secretions were additionally examined for Mycoplasma hominis , Ureaplasma urealyticum , Gardnerella vaginalis , other Gram-negative and Gram-positive bacteria, Trichomonas vaginalis , yeast-like fungi and leucocyte count. Furthermore, all patients were evaluated for the presence of serum anti-chlamydial IgG antibodies. Signs of inflammation on the basis of the count of leucocytes per hpf in the prostatic secretions were detected in 42 patients (group I). Prostatodynia was found in the remaining 36 men (group II). In group I, chlamydial antigen was detected in the urethra and expressed prostatic secretions (EPS) in 6 (14.3%) and 9 (21.4%) patients, respectively. No evidence of current chlamydial infection was found in group II. The presence of serum anti-chlamydial IgG antibodies was demonstrated in 13/42 (30.9%) patients with prostatitis and in 3/36 (8.3%) patients with prostatodynia ( P 0.01). The results suggest that chlamydia may be one of the causative agents of chronic prostatitis. <

scholarly journals Cervical Cytopathological Findings in Korean Women withChlamydia trachomatis,Mycoplasma hominis, andUreaplasma urealyticumInfections

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Yuri Choi ◽  
Jaesook Roh
Keyword(s):  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Cervical Screening ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Causal Role ◽  
Pap Smears ◽  
Korean Women ◽  
Cellular Change ◽  
Cytological Abnormalities

This is to investigate the cervical cytological abnormalities associated withChlamydia trachomatis,Mycoplasma hominis,Mycoplasma genitalium,andUreaplasma urealyticuminfections on routine screen. A total of 714 subjects who had undergone cervical Pap smears and concomitant analyses for cervical infections were included by a retrospective search. The frequencies of reactive cellular change (RCC) and squamous epithelial abnormalities were significantly higher inChlamydiapositive subjects than in uninfected subjects(P<0.001). Of the 124 subjects tested forM. hominis,M. genitalium, andU. urealyticum, 14 (11%) were positive forM. hominisand 29 (23%) were positive forU. urealyticum. Squamous abnormalities were more frequent in subjects withUreaplasmainfections than in uninfected subjects (24% versus 8%). Taking together these findings,C. trachomatisandU. urealyticummay have a causal role in the development of cervical epithelial changes, including RCC. Thus, extra awareness is warranted in cervical screening of women withChlamydiaorUreaplasmainfections.

scholarly journals Microbiological Characteristics of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in South African Women

2015 ◽  
Vol 54 (1) ◽  
pp. 200-203 ◽  
Author(s):  
Jan Henk Dubbink ◽  
Dewi J. de Waaij ◽  
Myrte Bos ◽  
Lisette van der Eem ◽  
Cécile Bébéar ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Cross Sectional Study ◽  
African Women ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Content Type ◽  
South African Women

We analyzed data of 263 women with at least one genital or anorectal sexually transmitted infection from a cross-sectional study conducted in rural South Africa. We provide new insights concerning the concurrence ofChlamydia trachomatis,Neisseria gonorrhoeae,Mycoplasma genitalium, andTrichomonas vaginalisinfections as well as the characteristics of bacterial loads.

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