Metabolic profiling of endogenous bile acids: a novel method to assess hepatoprotective effect of Tanreqing capsule on carbon-tetrachloride-induced liver injury in rats

Background: In absence of reliable hepatoprotective drug in modern medicine, the traditional herbal medicines have been emphasized. Present study was designed to assess hepatoprotective effect of aqueous extract of Melothria perpusilla (AEMP) against carbon tetrachloride (CCl4) induced liver injury.Methods: Five groups of animals with 6 rats in each were treated for 7 days. Group I received 1% gum acacia in distilled water (1 ml/200 g p.o.) daily. Group II, III, IV and V received CCl4 in liquid paraffin (1 ml/kg s.c.) on day 2, 4 and 6. Group III, IV and V were treated respectively with silymarin (100 mg/kg p.o.), AEMP- 200 and 400 mg/kg p.o. daily. On day 8, liver injury was assessed by measuring serum ALT, AST, ALP and bilirubin.Results: ALT, AST, ALP and bilirubin were significantly reduced in groups receiving both CCl4 and AEMP when compared with CCl4 treated group.Conclusions: AEMP produced hepatoprotective effect against CCl4 induced liver injury.

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Individual serum bile acids as early indicators of carbon tetrachloride- and chloroform-induced liver injury

Toxicology ◽

10.1016/0300-483x(92)90004-x ◽

1992 ◽

Vol 75 (3) ◽

pp. 221-234 ◽

Cited By ~ 26

Author(s):

Cheng-long Bai ◽

Paul J. Canfield ◽

Neill H. Stacey

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Bile Acids ◽

Serum Bile Acids ◽

Early Indicators

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Hepatoprotective Effect of Scoparia dulcis on Carbon Tetrachloride Induced Acute Liver Injury in Mice

The American Journal of Chinese Medicine ◽

10.1142/s0192415x10008226 ◽

2010 ◽

Vol 38 (04) ◽

pp. 761-775 ◽

Cited By ~ 14

Author(s):

Jen-Chieh Tsai ◽

Wen-Huang Peng ◽

Tai-Hui Chiu ◽

Shun-Chieh Huang ◽

Tai-Hung Huang ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

High Performance ◽

Acute Liver Injury ◽

Ethanol Extract ◽

Significant Inhibition ◽

Hepatoprotective Effect ◽

Oxidative Enzymes ◽

Active Constituents ◽

Scoparia Dulcis

This study aims to investigate the hepatoprotective activity and active constituents of the ethanol extract of Scoparia dulcis (SDE). The hepatoprotective effect of SDE (0.1, 0.5 and 1 g/kg) was evaluated on the carbon tetrachloride ( CCl4 )-induced acute liver injury. The active constituents were detected by high performance liquid chromatography (HPLC). Mice pretreated orally with SDE (0.5 and 1.0 g/kg) and silymarin (200 mg/kg) for five consecutive days before the administering of a single dose of 0.2% CCl4 (10 ml/kg of bw, ip) showed a significant inhibition of the increase of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Histological analyses also showed that SDE (0.5 and 1.0 g/kg) and silymarin reduced the extent of liver lesions induced by CCl4 , including vacuole formation, neutrophil infiltration and necrosis. Moreover, SDE decreased the malondialdehyde (MDA) level and elevated the content of reduced glutathione (GSH) in the liver as compared to those in the CCl4 group. Furthermore, SDE (0.5 and 1.0 g/kg) enhanced the activities of anti-oxidative enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd) and glutathione-S-transferase (GST). The quantities of active constituents in SDE were about 3.1 mg luteolin/g extract and 1.1 mg apigenin/g extract. The hepatoprotective mechanisms of SDE were likely associated to the decrease in MDA level and increase in GSH level by increasing the activities of antioxidant enzymes such as SOD, GPx, GRd and GST. These results demonstrated that SDE could alleviate CCl4 -induced acute liver injury in mice.

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Actinidia rubricaulisAttenuates Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

The American Journal of Chinese Medicine ◽

10.1142/s0192415x07004643 ◽

2007 ◽

Vol 35 (01) ◽

pp. 81-88 ◽

Cited By ~ 7

Author(s):

Jung-Chun Liao ◽

Kun-Hung Lin ◽

Hao-Yuan Cheng ◽

Jin-Bin Wu ◽

Ming-Tsuen Hsieh ◽

...

Keyword(s):

Antioxidant Activity ◽

Carbon Tetrachloride ◽

Liver Injury ◽

Experimental Period ◽

Hepatoprotective Effect ◽

Glutamate Pyruvate Transaminase ◽

Chronic Liver Injury ◽

Glutamate Pyruvate ◽

Ethanol Extracts ◽

Serum Glutamate

The purpose of this study was to evaluate the antioxidant activity and hepatoprotective effect of ethanol extracts of Actinidia rubricaulis (AR) on chronic liver injury induced by carbon tetrachloride ( CCl4) in rats. CCl4(20%, 0.5 ml/rat) was given twice a week for 8 weeks, and animals received AR throughout the entire experimental period. AR reduced the elevated levels of serum glutamate-oxalate-transaminase (sGOT) and glutamate-pyruvate-transaminase (sGPT) caused by CCl4at weeks 1,3,6, and 8. The biochemical data were consistent with those of the histological observations. The AR extract recovered the CCl4-induced liver injury and showed antioxidant effect in assays of antioxidant enzyme activity, such as SOD, GSH-Px and GSH-Rd. Based on these results, we suggest that the hepatoprotective effect of the AR is related to its antioxidant activity.

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