Modified Method of C Banding Using Barium Hydroxide

1975 ◽  
Vol 24 (3-4) ◽  
pp. 315-316
Author(s):  
P.K. Ghosh ◽  
Indera P. Singh
Keyword(s):  
Barium Hydroxide ◽  
Centromeric Heterochromatin ◽  
Banding Technique ◽  
C Banding ◽  
Modified Method ◽  
The Relationship

A modified centromeric heterochromatin banding technique using barium hydroxide octahydrate is described. The relationship between slide maturity and time of denaturation by barium-hydroxide is discussed.

DIFFERENTIAL GIEMSA STAINING IN PLANTS. VI. CENTROMERIC BANDING

1979 ◽  
Vol 21 (3) ◽  
pp. 373-378 ◽  
Author(s):  
W. Gary Filion ◽  
David H. Blakey
Keyword(s):  
Room Temperature ◽  
Chromosome Banding ◽  
Giemsa Staining ◽  
Barium Hydroxide ◽  
Banding Technique ◽  
Giemsa Banding ◽  
Metaphase Chromosomes ◽  
Somatic Metaphase ◽  
C Banding ◽  
Hydrolysis Temperature

Somatic metaphase chromosomes of Tulipa which were subjected to various hydrolyses with several times and temperatures displayed two distinctive types of C-banding when stained using the BSG (Barium hydroxide/Saline/Giemsa) chromosome banding technique. In addition to the two types of Giemsa bands, namely intercalary/terminal and centromeric, a unique transition from the former to the latter type of banding was observed. That is, at the point of transition from intercalary/terminal to centromeric banding, both types were present at one time. The two types of Giemsa banding resulted from different HCl hydrolysis times and temperatures; centromeric bands being observed after either a prolonged hydrolysis at room temperature or an increase in the hydrolysis temperature to 60 °C. These results are discussed in relation to the mechanisms of chromosome banding.

Use of faecal organisms from sheep for the in vitro determination of digestibility

1987 ◽  
Vol 109 (2) ◽  
pp. 257-259 ◽  
Author(s):  
H. M. El Shaer ◽  
H. M. Omed ◽  
A. G. Chamberlain ◽  
R. F. E. Axford
Keyword(s):  
Modified Method ◽  
Liquid Suspension ◽  
The Relationship

SummaryA method is described in which a liquid suspension of sheep faeces is used as an inoculum in the in vitro determination of digestibility of feedingstuffs for ruminants. The modified method was applied to 21 samples of grass, ten of lucerne, and a variety of other food materials. The results correlated closely (r = 0·98) with the in vivo digestibilities, and the relationship between in vitro and in vivo digestibilities was represented by the equation: in vivo digestibility = in vitro digestibility × 1·003.

SPECIFIC C BAND PATTERNS IN CONTINUOUS HUMAN LYMPHOBLASTOID CELL LINES

1974 ◽  
Vol 16 (2) ◽  
pp. 305-315 ◽  
Author(s):  
Frank J. O'Neill ◽  
Charles P. Miles
Keyword(s):  
Cell Lines ◽  
The Other ◽  
Chromosome 1 ◽  
Centromeric Heterochromatin ◽  
Banding Technique ◽  
Lymphoblastoid Cell Lines ◽  
Human Lymphoblastoid Cell ◽  
The One ◽  
Almost All ◽  
Human Lymphoblastoid Cell Lines

Analysis of centromeric heterochromatin in five human lymphoblastoid cell lines is described utilizing the C banding technique. Two lines, LK 60 and NC 37 showed a polymorphism for the size of the band on chromosome 1. LK 60 also showed accentuation or stretching of the secondary constriction on No. 1 and in almost all cells studied the affected homolog was also the one with the large C band. Another line SKL-1, also showed an accentuated constriction on chromosome 1 but did not have a detectable polymorphism. NC 37 did not show a constriction. In LK 60 the stretching of the constriction always appeared within the boundaries of the constitutive heterochromatin, regardless of the degree of stretching.SKL-1 and RPMI 6410 showed marker chromosomes with double C bands. One such chromosome appeared in SKL-1 and the bands were relatively widely spaced. However, analysis of this chromosome with standard staining procedures showed that one band was only rarely associated with a constriction while the other band, nearest the telomere, always showed a constriction. In RPMI 6410 two such markers were apparent. In one, the bands were well spaced, to allow an analysis for association with constrictions. In this case one band was always associated with a constriction while the other band showed a constriction in most of the cells. The possibility that these chromosomes are dicentric is discussed.

Karyotype characterization of the lake sturgeon, Acipenser fulvescens (Rafinesque 1817) by chromosome banding and fluorescent in situ hybridization

Genome ◽  
2004 ◽  
Vol 47 (4) ◽  
pp. 742-746 ◽  
Author(s):  
Francesco Fontana ◽  
Ronald M Bruch ◽  
Fred P Binkowski ◽  
Massimo Lanfredi ◽  
Milvia Chicca ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Lake Sturgeon ◽  
Centromeric Heterochromatin ◽  
Telomeric Sequence ◽  
Acipenser Fulvescens ◽  
Nucleolar Organizer Regions ◽  
C Banding

A karyotype analysis using several staining techniques was carried out on the North American lake sturgeon, Acipenser fulvescens. The chromosome number was found to be 2n = 262 ± 6. A representative karyotype of 264 chromosomes was composed of 134 meta- and submetacentrics, 70 telo- and acrocentrics, and 60 microchromosomes. The constitutive heterochromatin, revealed by C banding, was localized in various positions on several chromosomes, including microchromosomes. The signals of fluorescent in situ hybridization (FISH) with a HindIII satellite DNA probe were visible as centromeric heterochromatin blocks on 48 chromosomes. The telomeric repeat (TTAGGG)n detected by FISH was localized at both ends of all chromosomes and two chromosomes were entirely marked. Fluorescent staining with GC-specific chromomycin A3 showed recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific 4',6-diamidino-2-phenylindole (DAPI). After silver staining, the active nucleolar organizer regions (NORs) were detected on 12 chromosomes. FISH with the 5S probe showed four signals on four small chromosomes. Our data suggest that A. fulvescens is a tetraploid species.Key words: karyotype, C banding, telomeric sequence, fluorochrome staining, satellite DNA, 5S rDNA.

scholarly journals CHROMOSOME MARKERS IN MUS MUSCULUS: STRAIN DIFFERENCES IN C-BANDING

Genetics ◽  
1973 ◽  
Vol 75 (4) ◽  
pp. 663-670
Author(s):  
V G Dev ◽  
D A Miller ◽  
O J Miller
Keyword(s):  
Mus Musculus ◽  
Strain Differences ◽  
Inbred Strains ◽  
F1 Hybrids ◽  
Centromeric Heterochromatin ◽  
Mitotic Chromosomes ◽  
Homologous Chromosomes ◽  
Chromosome Markers ◽  
C Banding ◽  
Inbred Strains Of Mice

ABSTRACT The mitotic chromosomes of several inbred strains of mice and a series of F1 hybrids have been analyzed by quinacrine staining and further characterized by the centromeric heterochromatin banding (C-banding). Inbred strains had the same amount of C-banding material on homologous chromosomes but showed variation in the amount on different chromosomes. F1 hybrids showed characteristics of each parent and it appears that the amount of C-banding on each chromosome is a simple inherited polymorphism. In this study 12 different chromosomes could be distinguished by their C-banding, and these can be used as normal chromosome markers.

Modified Fermentation for Production of Bacterial Cellulose/Polyaniline as Conductive Biopolymer Material

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Norhayati Pa’e ◽  
Khairul Azly Zahan ◽  
Ida Idayu Muhamad ◽  
Kok Fook Seng
Keyword(s):  
Bacterial Cellulose ◽  
Acetobacter Xylinum ◽  
Operating Conditions ◽  
High Mechanical Strength ◽  
Modified Method ◽  
Conductive Film ◽  
Hydrophilic Material ◽  
Polyaniline Films ◽  
The Relationship ◽  
Film Conductivity

Bacterial cellulose is a pure and highly hydrophilic material with high mechanical strength. Furthermore by adding certain substrates or by manipulating the operating conditions it is possible to change the properties of the bacterial cellulose. The objective of this research is to develop a modified method for fabrication of bacterial cellulose-polyaniline films. Polyaniline was incorporated into bacterial cellulose during fermentation by Acetobacter xylinum in order to produce conductive film. Bacterial cellulose/polyaniline film was synthesized with addition of polyaniline in Rotary-discs reactor during bacterial cellulose formation. The conductivity of film produced was measured and compared. The effects of polyaniline concentration were studied and the relationship between polyaniline concentration and film conductivity were determined.

Homoeologous pairing and recombination between the long arms of group 1 chromosomes in wheat × rye hybrids

Genome ◽  
1989 ◽  
Vol 32 (2) ◽  
pp. 293-301 ◽  
Author(s):  
T. Naranjo ◽  
P. Fernández-Rueda ◽  
P. G. Goicoechea ◽  
A. Roca ◽  
R. Giráldez
Keyword(s):  
Banding Pattern ◽  
Homoeologous Pairing ◽  
Parental Type ◽  
C Banding ◽  
Group 1 Chromosomes ◽  
The Relationship ◽  
Double Recombinant ◽  
Group 1 ◽  
Metaphase I

The relationship between homoeologous pairing at metaphase I and recombination at anaphase I between the arms 1AL, 1BL, 1DL, and 1RL was analyzed in ph1b, 5B-deficient, and ph2b wheat × rye hybrids. All four arms could be identified at metaphase I, as well as the arms 1BL and 1RL at anaphase I, by means of C-banding. On the basis of the C-heterochromatin constitution that 1BL and 1RL showed at anaphase I and that association at metaphase I was essentially homoeologous, the following anaphase I chromosome types could be distinguished: parental type, single and double recombinant types between 1BL and 1AL or 1DL, between 1BL and 1RL, and between 1RL and 1AL or 1DL. Recombinant types 1AL – 1DL did not differ from the parental type for the C-banding pattern and was not considered. In the three genotypes, most if not all of 1BL – 1AL, 1BL – 1DL, and 1BL – 1RL metaphase I bonds were chiasmatic. 1RL – 1AL and 1RL – 1DL associations were scarce. Frequencies of one chiasma and two chiasmata for the arm combinations 1BL – 1AL plus 1BL – 1DL, 1BL – 1RL, and 1RL – 1AL plus 1RL – 1DL were estimated. Values decreased in the order ph1b, 5B-deficient, and ph2b hybrids.Key words: C-banding, chiasmata, homoeologues, anaphase I, ph genes.

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