scholarly journals Dietary protein intake affects expression of genes for lipid metabolism in porcine skeletal muscle in a genotype-dependent manner

2015 ◽  
Vol 113 (7) ◽  
pp. 1069-1077 ◽  
Author(s):  
Yingying Liu ◽  
Fengna Li ◽  
Lingyun He ◽  
Bie Tan ◽  
Jinping Deng ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Dietary Protein ◽  
Protein Intake ◽  
Dependent Manner ◽  
Dietary Protein Intake ◽  
Lipogenic Genes ◽  
Expression Of Genes ◽  
Mini Pigs ◽  
Mrna Expression Levels

Skeletal muscle is a major site for the oxidation of fatty acids (FA) in mammals, including humans. Using a swine model, we tested the hypothesis that dietary protein intake regulates the expression of key genes for lipid metabolism in skeletal muscle. A total of ninety-six barrows (forty-eight pure-bred Bama mini-pigs (fatty genotype) and forty-eight Landrace pigs (lean genotype)) were fed from 5 weeks of age to market weight. Pigs of fatty or lean genotype were randomly assigned to one of two dietary treatments (low- or adequate-protein diet), with twenty-four individually fed pigs per treatment. Our data showed that dietary protein levels affected the expression of genes involved in the anabolism and catabolism of lipids in the longissimus dorsi and biceps femoris muscles in a genotype-dependent manner. Specifically, Bama mini-pigs had more intramuscular fat, SFA and MUFA, as well as elevated mRNA expression levels of lipogenic genes, compared with Landrace pigs. In contrast, Bama mini-pigs had lower mRNA expression levels of lipolytic genes than Landrace pigs fed an adequate-protein diet in the growing phase. These data are consistent with higher white-fat deposition in Bama mini-pigs than in Landrace pigs. In conclusion, adequate provision of dietary protein (amino acids) plays an important role in regulating the expression of key lipogenic genes, and the growth of white adipose tissue, in a genotype- and tissue-specific manner. These findings have important implications for developing novel dietary strategies in pig production.

scholarly journals PSIII-27 Dietary intakes of protein and starch affect the oxidation of nutrients in tissues of Largemouth bass

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 275-275
Author(s):  
Xinyu Li ◽  
Guoyao Wu
Keyword(s):  
Skeletal Muscle ◽  
Dietary Protein ◽  
Largemouth Bass ◽  
Protein Intake ◽  
Micropterus Salmoides ◽  
Nitrogen Retention ◽  
Dietary Protein Intake ◽  
Dietary Intakes ◽  
Protein Levels ◽  
Energy Retention

Abstract Largemouth bass (LMB, Micropterus salmoides) is a carnivorous fish. This study was conducted to test the hypothesis that dietary intakes of protein and starch affect the oxidation of nutrients in the intestine, liver, skeletal muscle and kidneys of LMB. Juvenile LMB (average initial weight = 18 g) were fed, for 8 weeks, fish meal- and soy protein concentrate-based diets containing 40%, 45% and 50% crude protein. The three isocaloric diets contained 22.3%, 15.78% and 9.2% dextrinized starch, respectively. The growth performance, energy retention, and lipid retention in LMB increased (P < 0.05) with increasing dietary protein levels, but the efficiency of nitrogen retention did not differ (P > 0.05) among the three groups of fish. Rates of oxidation of 2 mM glutamate and glutamine in the intestine were reduced (P < 0.05) in response to increased dietary protein intake. This adaptive metabolic change likely helps to prevent excessive production of ATP by the gut when the dietary provision of glutamate and glutamine was increased. Increasing dietary protein intake did not affect (P > 0.05) glutamate oxidation in the liver, kidneys or skeletal muscle, or glutamine oxidation in the muscle. In contrast, the rate of glutamine oxidation was increased (P < 0.05) in the liver but decreased (P < 0.05) in the kidneys as dietary protein intake was elevated from 40% to 50%. Rates of oxidation of 5 mM glucose were reduced (P < 0.05) in the liver, kidneys and intestine, but were not altered in skeletal muscle, in response to reduced starch intake. Rates of oxidation of 2 mM palmitate were reduced (P < 0.05) in the kidneys but were not influenced (P > 0.05) in the intestine, skeletal muscle or liver. We conclude that oxidation of energy substrates in LMB tissues is regulated by dietary protein and starch intake in a tissue-specific manner.

Dietary Protein Intake and Distribution Patterns of Well-Trained Dutch Athletes

2017 ◽  
Vol 27 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Jenna B. Gillen ◽  
Jorn Trommelen ◽  
Floris C. Wardenaar ◽  
Naomi Y.J. Brinkmans ◽  
Joline J. Versteegen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Total Energy ◽  
Energy Intake ◽  
Dietary Protein ◽  
Adaptive Response ◽  
Protein Intake ◽  
Team Sport ◽  
Total Energy Intake ◽  
Food Sources ◽  
Dietary Protein Intake

Dietary protein intake should be optimized in all athletes to ensure proper recovery and enhance the skeletal muscle adaptive response to exercise training. In addition to total protein intake, the use of specific proteincontaining food sources and the distribution of protein throughout the day are relevant for optimizing protein intake in athletes. In the present study, we examined the daily intake and distribution of various proteincontaining food sources in a large cohort of strength, endurance and team-sport athletes. Well-trained male (n=327) and female (n=226) athletes completed multiple web-based 24-hr dietary recalls over a 2-4 wk period. Total energy intake, the contribution of animal- and plant-based proteins to daily protein intake, and protein intake at six eating moments were determined. Daily protein intake averaged 108±33 and 90±24 g in men and women, respectively, which corresponded to relative intakes of 1.5±0.4 and 1.4±0.4 g/kg. Dietary protein intake was correlated with total energy intake in strength (r=0.71, p <.001), endurance (r=0.79, p <.001) and team-sport (r=0.77, p <.001) athletes. Animal and plant-based sources of protein intake was 57% and 43%, respectively. The distribution of protein intake was 19% (19±8 g) at breakfast, 24% (25±13 g) at lunch and 38% (38±15 g) at dinner. Protein intake was below the recommended 20 g for 58% of athletes at breakfast, 36% at lunch and 8% at dinner. In summary, this survey of athletes revealed they habitually consume > 1.2 g protein/kg/d, but the distribution throughout the day may be suboptimal to maximize the skeletal muscle adaptive response to training.

scholarly journals Dietary protein intake impacts human skeletal muscle protein fractional synthetic rates after endurance exercise

2005 ◽  
Vol 289 (4) ◽  
pp. E678-E683 ◽  
Author(s):  
Douglas R. Bolster ◽  
Matthew A. Pikosky ◽  
P. Courtney Gaine ◽  
William Martin ◽  
Robert R. Wolfe ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Endurance Exercise ◽  
Dietary Protein ◽  
Protein Intake ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Dietary Protein Intake ◽  
Skeletal Muscle Protein ◽  
Skeletal Muscle Protein Synthesis

This investigation evaluated the physiological impact of different dietary protein intakes on skeletal muscle protein synthesis postexercise in endurance runners. Five endurance-trained, male runners participated in a randomized, crossover design diet intervention, where they consumed either a low (0.8 g/kg; LP)-, moderate (1.8 g/kg; MP)-, or high (3.6 g/kg; HP)-protein diet for 4 wk. Diets were designed to be eucaloric with carbohydrate, fat, and protein approximating 60, 30, and 10%; 55, 30, and 15%; and 40, 30, and 30% for LP, MP, and HP, respectively. Substrate oxidation was assessed via indirect calorimetry at 3 wk of the dietary interventions. Mixed-muscle protein fractional synthetic rate (FSR) was measured after an endurance run (75 min at 70% V̇o2 peak) using a primed, continuous infusion of [2H5]phenylalanine. Protein oxidation increased with increasing protein intake, with each trial being significantly different from the other ( P < 0.01). FSR after exercise was significantly greater for LP (0.083%/h) and MP (0.078%/h) than for HP (0.052%/h; P < 0.05). There was no difference in FSR between LP and MP. This is the first investigation to establish that habitual dietary protein intake in humans modulates skeletal muscle protein synthesis after an endurance exercise bout. Future studies directed at mechanisms by which level of protein intake influences skeletal muscle turnover are needed.

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