scholarly journals Dietary carbohydrate and lipid sources affect differently the oxidative status of European sea bass (Dicentrarchus labrax) juveniles

2015 ◽  
Vol 114 (10) ◽  
pp. 1584-1593 ◽  
Author(s):  
Carolina Castro ◽  
Amalia Peréz-Jiménez ◽  
Filipe Coutinho ◽  
Patricia Díaz-Rosales ◽  
Cláudia Alexandra dos Reis Serra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Dietary Lipid ◽  
Oxidative Status ◽  
Fish Diet ◽  
Dietary Carbohydrate ◽  
European Sea Bass ◽  
Lipid Source ◽  
Dietary Lipid Source

AbstractThis study aimed to evaluate the effects of dietary lipid source and carbohydrate content on the oxidative status of European sea bass (Dicentrarchus labrax) juveniles. For that purpose, four diets were formulated with fish oil (FO) and vegetable oils (VO) as the lipid source and with 20 or 0 % gelatinised starch as the carbohydrate source, in a 2×2 factorial design. Liver and intestine antioxidant enzyme activities (catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD)), hepatic and intestinal lipid peroxidation (LPO), as well as hepatic oxidative stress index (OSI), were measured in fish fed the experimental diets for 73 d (n9 fish/diet). Carbohydrate-rich diets promoted a decrease in hepatic LPO and OSI, whereas the lipid source induced no changes. Inversely, dietary lipid source, but not dietary carbohydrate concentration, affected LPO in the intestine. Lower intestinal LPO was observed in VO groups. Enzymes responsive to dietary treatments were GR, G6PD and CAT in the liver and GR and GPX in the intestine. Dietary carbohydrate induced GR and G6PD activities and depressed CAT activity in the liver. GPX and GR activities were increased in the intestine of fish fed VO diets. Overall, effects of diet composition on oxidative status were tissue-related: the liver and intestine were strongly responsive to dietary carbohydrates and lipid sources, respectively. Furthermore, different metabolic routes were more active to deal with the oxidative stress in the two organs studied.

scholarly journals Dietary Phytogenics and Galactomannan Oligosaccharides in Low Fish Meal and Fish Oil-Based Diets for European Sea Bass (Dicentrarchus labrax) Juveniles: Effects on Gill Structure and Health and Implications on Oxidative Stress Status

2021 ◽  
Vol 12 ◽  
Author(s):  
Silvia Torrecillas ◽  
Genciana Terova ◽  
Alex Makol ◽  
Antonio Serradell ◽  
Victoria Valdenegro-Vega ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fish Oil ◽  
Fish Meal ◽  
Raw Materials ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Control Group ◽  
Plant Origin ◽  
European Sea Bass ◽  
Oxidative Stress Status

An effective replacement for fish meal (FM) and fish oil (FO) based on plant-based raw materials in the feed of marine fish species is necessary for the sustainability of the aquaculture sector. However, the use of plant-based raw materials to replace FM and FO has been associated with several negative health effects, some of which are related to oxidative stress processes that can induce functional and morphological alterations in mucosal tissues. This study aimed to evaluate the effects of dietary oligosaccharides of plant origin (5,000 ppm; galactomannan oligosaccharides, GMOS) and a phytogenic feed additive (200 ppm; garlic oil and labiatae plant extract mixture, PHYTO) on the oxidative stress status and mucosal health of the gills of juvenile European sea bass (Dicentrarchus labrax). The experimental diets, low FM and FO diets (10%FM/6%FO) were supplemented with GMOS from plant origin and PHYTO for 63 days. GMOS and PHYTO did not significantly affect feed utilization, fish growth, and survival. GMOS and PHYTO downregulated the expression of β-act, sod, gpx, cat, and gr in the gills of the fish compared with that in fish fed the control diet. The expression of hsp70 and ocln was upregulated and downregulated, respectively, in the GMOS group compared with that in the control group, whereas the expression of zo-1 was downregulated in the PHYTO group compared with that in the GMOS group. The morphological, histopathological, immunohistochemical, and biochemical parameters of the fish gills were mostly unaffected by GMOS and PHYTO. However, the PHYTO group had lower incidence of lamellar fusion than did the control group after 63 days. Although the tissular distribution of goblet cells was unaffected by GMOS and PHYTO, goblet cell size showed a decreasing trend (−11%) in the GMOS group. GMOS and PHYTO significantly reduced the concentration of PCNA+ in the epithelium of the gills. The above findings indicated that GMOS and PHYTO in low FM/FO-based diets protected the gill epithelia of D. labrax from oxidative stress by modulating the expression of oxidative enzyme-related genes and reducing the density of PCNA+ cells in the gills of the fish.

Nutritional history does not modulate hepatic oxidative status of European sea bass (Dicentrarchus labrax) submitted to handling stress

2018 ◽  
Vol 44 (3) ◽  
pp. 911-918 ◽  
Author(s):  
Carolina Castro ◽  
Amalia Peréz-Jiménez ◽  
Filipe Coutinho ◽  
Geneviève Corraze ◽  
Stéphane Panserat ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Oxidative Status ◽  
European Sea Bass ◽  
Handling Stress

Dietary lipid composition affects blood leucocyte fatty acid compositions and plasma eicosanoid concentrations in European sea bass (Dicentrarchus labrax)

Aquaculture ◽  
1999 ◽  
Vol 179 (1-4) ◽  
pp. 335-350 ◽  
Author(s):  
Bruce M Farndale ◽  
J.Gordon Bell ◽  
Michael P Bruce ◽  
Niall R Bromage ◽  
Ferdinand Oyen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Composition ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Dietary Lipid ◽  
European Sea Bass ◽  
Fatty Acid Compositions

scholarly journals Productive Results, Oxidative Stress and Contaminant Markers in European Sea Bass: Conventional vs. Organic Feeding

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1226
Author(s):  
Antonio Carminato ◽  
Francesco Pascoli ◽  
Angela Trocino ◽  
Lisa Locatello ◽  
Lisa Maccatrozzo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Pcr Analysis ◽  
Positive Trend ◽  
Fish Fillet ◽  
European Sea Bass ◽  
Fish Flesh ◽  
Cytochrome P4501a ◽  
Conventional Diet

In the present study European sea bass (Dicentrarchus labrax) subjected to two different diets (organic vs. conventional) were evaluated in terms of growing performances, oxidative stress, and contaminant markers. Growing performances were evaluated using biometric measures and condition factor (K), whereas insulin-like growth factor (IGF-I and IGF-II) levels were assessed trough Real-Time PCR analysis. For oxidative stress, immunohistochemical staining for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal (HNE) was performed, whereas total glutathione (GSH) in blood serum was determined by an enzymatic method adapted. Cytochrome P4501A (CYP1A) and melanomacrophage centers (MMCs) were evaluated as contaminant markers trough immunohistochemical and histochemical approaches, respectively. The growing performances showed a positive trend in both groups but a greater productivity in conventional fed fish compared to the organic ones. A significant higher expression of MMCs was observed in organic vs. conventional diet fed fish. Fillet analysis showed a higher MUFA content and a lower PUFAs n-6 content in organically fed sea bass indicating that diets with a content in fatty acids closer to that of wild fish will definitely affect the fatty acid profile of the fish flesh. On the other hand, the diet composition did not seem to affect neither the oxidative stress parameters (GSH, 8-OHdG, HNE) nor the CYP1A expression.

scholarly journals Dietary carbohydrate and lipid source affect cholesterol metabolism of European sea bass (Dicentrarchus labrax) juveniles

2015 ◽  
Vol 114 (8) ◽  
pp. 1143-1156 ◽  
Author(s):  
Carolina Castro ◽  
Geneviève Corraze ◽  
Amalia Pérez-Jiménez ◽  
Laurence Larroquet ◽  
Marianne Cluzeaud ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Cholesterol Metabolism ◽  
Sea Bass ◽  
Whole Body ◽  
Plasma Metabolites ◽  
Hepatic Glycogen ◽  
Transcriptional Level ◽  
Dietary Carbohydrate ◽  
European Sea Bass ◽  
Lipid Source

AbstractPlant feedstuffs (PF) are rich in carbohydrates, which may interact with lipid metabolism. Thus, when considering dietary replacement of fishery by-products with PF, knowledge is needed on how dietary lipid source (LS) and carbohydrates affect lipid metabolism and other metabolic pathways. For that purpose, a 73-d growth trial was performed with European sea bass juveniles (IBW 74 g) fed four diets differing in LS (fish oil (FO) or a blend of vegetable oils (VO)) and carbohydrate content (0 % (CH–) or 20 % (CH+) gelatinised starch). At the end of the trial no differences among diets were observed on growth and feed utilisation. Protein efficiency ratio was, however, higher in the CH+ groups. Muscle and liver fatty acid profiles reflected the dietary LS. Dietary carbohydrate promoted higher plasma cholesterol and phospholipids (PL), whole-body and hepatic (mainly 16 : 0) lipids and increased muscular and hepatic glycogen. Except for PL, which were higher in the FO groups, no major alterations between FO and VO groups were observed on plasma metabolites (glucose, TAG, cholesterol, PL), liver and muscle glycogen, and lipid and cholesterol contents. Activities of glucose-6-phosphate dehydrogenase and malic enzyme – lipogenesis-related enzymes – increased with carbohydrate intake. Hepatic expression of genes involved in cholesterol metabolism was up-regulated with carbohydrate (HMGCR and CYP3A27) and VO (HMGCR and CYP51A1) intake. No dietary regulation of long-chain PUFA biosynthesis at the transcriptional level was observed. Overall, very few interactions between dietary carbohydrates and LS were observed. However, important insights on the direct relation between dietary carbohydrate and the cholesterol biosynthetic pathway in European sea bass were demonstrated.

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