Opisthorchis viverrini-like liver fluke in birds from Vietnam: morphological variability and rDNA/mtDNA sequence confirmation

2013 ◽  
Vol 88 (4) ◽  
pp. 441-446 ◽  
Author(s):  
T.H. Dao ◽  
T.G. Nguyen ◽  
B. Victor ◽  
S. Gabriël ◽  
P. Dorny
Keyword(s):  
Phylogenetic Trees ◽  
Liver Fluke ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Reservoir Host ◽  
Opisthorchis Viverrini ◽  
Domestic Ducks ◽  
Central Vietnam ◽  
Mtdna Sequence ◽  
Epidemiological Situation

AbstractFlukes were found in the bile ducts of domestic ducks (Anas platyrhynchos), necropsied in the Binh Dinh province of Central Vietnam. Following staining, morphological characteristics of the bird flukes were compatible with Opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. Computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (ITS2) of the ribosomal DNA and cytochrome c oxidase subunit I (COI) markers of the mitochondrial DNA showed close similarity of the ‘bird’ Opisthorchis sp. with O. viverrini. We speculate that these bird flukes are O. viverrini that show intraspecies morphological and molecular variability compared to isolates from mammals. This demonstrates the complex epidemiological situation of opisthorchiasis in Vietnam and urges investigations on the potential of birds as a reservoir host of this zoonotic fluke.

Is Opisthorchis viverrini an avian liver fluke?

2013 ◽  
Vol 89 (2) ◽  
pp. 255-256 ◽  
Author(s):  
Y. Nawa ◽  
P.N. Doanh ◽  
U. Thaenkham
Keyword(s):  
Bile Duct ◽  
Cytochrome Oxidase ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Liver Fluke ◽  
First Record ◽  
Opisthorchis Viverrini ◽  
Gene Sequences ◽  
Domestic Ducks ◽  
Liver Flukes

AbstractRecently, in the Journal of Helminthology (May 2013), Dao et al. reported that Opisthorchis viverrini-like flukes were found in the bile duct of domestic ducks in Vietnam. They stated that this is the first record of Opisthorchis sp. in birds in Vietnam. However, three Opisthorchis species – O. cheelis, O. longissimus and O. parageminus – in birds in Vietnam were described by Le in 2000. Amongst these, O. parageminus was first reported, by Oshmarin in 1970, as a new Opisthorchis species found in domestic ducks (Anas platyrhynchos) in Vietnam. Morphologially O. viverrini-like flukes described by Dao et al. are much more similar to O. parageminus than to O. viverrini. The phylogenetic trees of internal transcribed spacer 2 (ITS2) and cytochrome oxidase 1 (CO1) gene sequences also showed that the O. viverrini-like liver flukes from domestic ducks were closer to O. lobatus than to O. viverrini. Therefore, O. viverrini-like liver flukes reported by Dao et al. (2013) are most likely to be O. parageminus.

scholarly journals Characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, Opisthorchis viverrini

2008 ◽  
Vol 160 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Sutas Suttiprapa ◽  
Alex Loukas ◽  
Thewarach Laha ◽  
Sopit Wongkham ◽  
Sasithorn Kaewkes ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Human Liver ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Thioredoxin Peroxidase

scholarly journals Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 189 ◽  
Author(s):  
Thewarach Laha ◽  
Porntip Pinlaor ◽  
Jason Mulvenna ◽  
Banchob Sripa ◽  
Manop Sripa ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Fluke ◽  
Gene Discovery ◽  
Opisthorchis Viverrini

scholarly journals Suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, Opisthorchis viverrini

2014 ◽  
Vol 7 (1) ◽  
pp. 224 ◽  
Author(s):  
Sirikanda Thanasuwan ◽  
Supawadee Piratae ◽  
Paul J Brindley ◽  
Alex Loukas ◽  
Sasithorn Kaewkes ◽  
...  
Keyword(s):  
Liver Fluke ◽  
Water Channel ◽  
Opisthorchis Viverrini ◽  
Channel Control

scholarly journals Suppression of mRNAs encoding CD63 family tetraspanins from the carcinogenic liver fluke Opisthorchis viverrini results in distinct tegument phenotypes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sujittra Chaiyadet ◽  
Watchara Krueajampa ◽  
Wiphawi Hipkaeo ◽  
Yada Plosan ◽  
Supawadee Piratae ◽  
...  
Keyword(s):  
Liver Fluke ◽  
Opisthorchis Viverrini

Opisthorchis viverrini: Gene expression profiling of carcinogenic adult liver fluke worms using 5′ SAGE

2008 ◽  
Vol 120 (4) ◽  
pp. 306-313 ◽  
Author(s):  
Nopporn Chutiwitoonchai ◽  
Yan Shen ◽  
Huajun Zheng ◽  
Hui Xiong ◽  
Guoping Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Adult Liver

scholarly journals Current Classification and Diversity of Fusarium Species Complex, the Causal Pathogen of Fusarium Wilt Disease of Banana in Malaysia

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1955
Author(s):  
Anysia Hedy Ujat ◽  
Ganesan Vadamalai ◽  
Yukako Hattori ◽  
Chiharu Nakashima ◽  
Clement Kiing Fook Wong ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Species Complex ◽  
Phylogenetic Trees ◽  
Fusarium Species ◽  
Morphological Characteristics ◽  
Pathogenicity Test ◽  
Fusarium Spp ◽  
Specific Primers ◽  
Fusarium Oxysporum Species Complex

The re-emergence of the Fusarium wilt caused by Fusarium odoratissimum (F. odoratissimum) causes global banana production loss. Thirty-eight isolates of Fusarium species (Fusarium spp.) were examined for morphological characteristics on different media, showing the typical Fusarium spp. The phylogenetic trees of Fusarium isolates were generated using the sequences of histone gene (H3) and translation elongation factor gene (TEF-1α). Specific primers were used to confirm the presence of F. odoratissimum. The phylogenetic trees showed the rich diversity of the genus Fusarium related to Fusarium wilt, which consists of F. odoratissimum, Fusarium grosmichelii, Fusarium sacchari, and an unknown species of the Fusarium oxysporum species complex. By using Foc-TR4 specific primers, 27 isolates were confirmed as F. odoratissimum. A pathogenicity test was conducted for 30 days on five different local cultivars including, Musa acuminata (AAA, AA) and Musa paradisiaca (AAB, ABB). Although foliar symptoms showed different severity of those disease progression, vascular symptoms of the inoculated plantlet showed that infection was uniformly severe. Therefore, it can be concluded that the Fusarium oxysporum species complex related to Fusarium wilt of banana in Malaysia is rich in diversity, and F. odoratissimum has pathogenicity to local banana cultivars in Malaysia regardless of the genotype of the banana plants.

scholarly journals Characterization of Five New Earthworm Mitogenomes (Oligochaeta: Lumbricidae): Mitochondrial Phylogeny of Lumbricidae

Diversity ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 580
Author(s):  
Hongyi Liu ◽  
Yufeng Zhang ◽  
Wei Xu ◽  
Yu Fang ◽  
Honghua Ruan
Keyword(s):  
Phylogenetic Trees ◽  
Mitochondrial Gene ◽  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Phylogenetic Studies ◽  
Octolasion Tyrtaeum ◽  
Eisenia Nordenskioldi ◽  
Novel Strategy

Identification based on conventional morphological characteristics is typically difficult and time-consuming. The development of molecular techniques provides a novel strategy that relies on specific mitochondrial gene fragments to conduct authentication. For this study, five newly sequenced partial mitogenomes of earthworms (Bimastos parvus, Dendrobaena octaedra, Eisenia andrei, Eisenia nordenskioldi, and Octolasion tyrtaeum) with lengths ranging from 14,977 to 15,715 were presented. Each mitogenome possessed a putative control region that resided between tRNA-Arg and tRNA-His. All of the PCGs were under negative selection according to the value of Ka/Ks. The phylogenetic trees supported the classification of Eisenia and Lumbricus; however, the trees based on cox1 did not. Through various comparisons, it was determined that cox1 fragments might be more suitable for molecular identification. These results lay the foundation for further phylogenetic studies on Lumbricidae.

Attempts to induce protective immunity in hamsters against infection by a liver fluke of man (Opisthorchis viverrini)

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 127-136 ◽  
Author(s):  
S. Sirisinha ◽  
S. Tuti ◽  
A. Tawatsin ◽  
S. Vichasri ◽  
E. S. Upatham ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Liver Fluke ◽  
Acquired Resistance ◽  
Opisthorchis Viverrini ◽  
Chronic Infections ◽  
Subsequent Infection ◽  
Worm Recovery ◽  
Small Doses ◽  
Prior Infection

SUMMARYThe development of acquired resistance in opisthorchiasis was studied in hamsters experimentally infected with Opisthorchis viverrini. The induction of protective immunity was attempted by first exposing adult female golden Syrian hamsters to 1,2 or 3 doses of infective metacercariae obtained from naturally infected cyprinoid fishes and then reinfecting them with 80 metacercariae. In other experiments, animals that were infected with 50 metacercariae were treated with praziquantel prior to being rechallenged in order to eliminate the flukes that had developed from the first infection. The effect of long-term chronic infections was also studied. Faecal egg counts were determined at weekly intervals from 4–5 weeks onwards. The animals were killed 2–3 months after the last infection for worm recovery, and terminal faecal egg output/g faeces/worm was calculated. The data showed that prior infection of animals with O. viverrini did not induce significant protective immunity against reinfection by the same parasite. Lack of protection was also noted in animals reinfected several times with small doses of metacercariae. However, under certain circumstances, prior infection could result in a significant reduction in the faecal egg output due to subsequent infection.

The genus Cystoseira s.l. (Ochrophyta, Fucales, Sargassaceae) in the Black Sea: morphological variability and molecular taxonomy of Gongolaria barbata and endemic Ericaria crinita f. bosphorica comb. nov.

Phytotaxa ◽  
2021 ◽  
Vol 480 (1) ◽  
pp. 1-21
Author(s):  
SOFIA S. SADOGURSKA ◽  
JOÃO NEIVA ◽  
ANNALISA FALACE ◽  
ESTER A. SERRÃO ◽  
ÁLVARO ISRAEL
Keyword(s):  
Mediterranean Sea ◽  
Black Sea ◽  
Molecular Taxonomy ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Morphological Data ◽  
The Black Sea ◽  
Morphological Identification ◽  
Additional Tool

Brown algae of the genus Cystoseira sensu lato form the most diverse and productive marine ecosystems throughout the Mediterranean Sea and have equal roles also in the Black Sea where they have been decreasing in the recent years. The taxonomy of Cystoseira s.l. taxa from the Black Sea is still not well understood, and questions arise when related taxa have to be delimited. In addition to morphological descriptions, this study provides for the first time molecular data of the Black Sea Cystoseira s.l. distinct morphologies as an additional tool to clarify their identities and phylogenetic affinities. The analysis of two mitochondrial markers (cytochrome oxidase subunit 1—COI, and 23S-tRNAVal intergenic spacer—mt-spacer) showed that Cystoseira s.l. specimens from the Black Sea belong to two recently resurrected genera, namely Gongolaria and Ericaria. Molecular data confirm the morphological identification of G. barbata, which is characterized by high morphological plasticity in the Black Sea. The morphological data presented in this study support the transition of G. barbata to the genus Gongolaria, which was previously proposed based solely on genetic data. For the Black Sea endemic taxon C. bosphorica, sequence divergence suggests conspecificity with Mediterranean Sea species E. crinita and E. barbatula. However, considering original morphological characteristics of the taxon, its geographical isolation, and endemism, the new combination Ericaria crinita f. bosphorica comb. nov. is proposed.

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