Levels of sparganum infections and phylogenetic analysis of the tapeworm Spirometra erinaceieuropaei sparganum in wild frogs from Henan Province in central China

2014 ◽  
Vol 89 (4) ◽  
pp. 433-438 ◽  
Author(s):  
T. Wei ◽  
X. Zhang ◽  
J. Cui ◽  
L.N. Liu ◽  
P. Jiang ◽  
...  

AbstractSparganosis is a serious food-borne parasitic zoonosis caused by infection with Spirometra spargana. The prevalence of sparganum infection in wild frogs (Rana nigromaculata, R. limmochari, R. temporaria and Bufo gargarizans) was investigated in Henan Province of central China during 2008–2012. Of 3482 caught wild frogs, 565 (16.23%) were found to be infected with plerocercoids (spargana) of the genus Spirometra. Spargana were found in 14.85% (320/2155) of R. nigromaculata, 20.82% (233/1119) of R. limmochari and 10.91% (12/110) of R. temporaria frogs. However, no sparganum was found in B. gargarizans. To investigate the phylogenetic position of collected spargana, three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunits 1 and 3 (cox1 and cox3), and NADH dehydrogenase subunit 4 (nad4), were amplified, sequenced and analysed. Sequences of cox1, cox3 and pnad4 were 417, 390 and 578 bp in length, respectively. The base composition of cox1, cox3 and pnad4 were generally AT rich with a mean of 63.5%, 68.3% and 67% AT, respectively. Phylogenetic analysis showed that all the sparganum isolates in Henan Province represented Spirometra erinaceieuropaei and were a well-supported clade. These findings demonstrated clearly the usefulness of the three mtDNA sequences for molecular identification and population genetics studies of S. erinaceieuropaei spargana of human and animal health significance.

2011 ◽  
Vol 86 (3) ◽  
pp. 271-275 ◽  
Author(s):  
W. Liu ◽  
G.H. Liu ◽  
F. Li ◽  
D.S. He ◽  
T. Wang ◽  
...  

AbstractSequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 3 (cox3), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) in Spirometra erinaceieuropaei spargana from different geographical regions in China was examined. A portion of each of the cox3 (pcox3), nad1 (pnad1) and nad4 genes (pnad4) were amplified separately from individual S. erinaceieuropaei spargana by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. The sequences of pcox3, pnad1 and pnad4 were 541, 607 and 847 bp in length, respectively. The A+T contents of the sequences were 68.39–68.76% (pcox3), 63.76–64.91% (pnad1) and 67.18–67.77% (pnad4), respectively, while the intra-specific sequence variations within each of the S. erinaceieuropaei spargana were 0–1.5% for pcox3, 0–2.8% for pnad1 and 0–2.7% for pnad4. Phylogenetic analysis using neighbour joining (NJ), maximum likelihood (ML) and maximum parsimony (MP) methods, indicated that all the spargana isolates in Hunan Province represented S. erinaceieuropaei. These findings demonstrated clearly the usefulness of the three mtDNA sequences for population genetics studies of S. erinaceieuropaei spargana of human and animal health significance.


Author(s):  
Sterling J. Nesbitt ◽  
Jun Liu ◽  
Chun Li

ABSTRACTThe two major lineages of extant archosaurs, crocodylians and avians, diverged in the Triassic, but the details and timing of this event are incompletely understood. Fragmentary and phylogenetically uninformative specimens, in addition to poor temporal constraints on rock units from the Early and Middle Triassic, typify obstacles in identifying early archosaurs. This paper re-describes the partial skeleton of the only known specimen of Xilousuchus sapingensis Wu, 1981 from the Early Triassic Heshanggou Formation in north-central China. Originally assigned to the non-archosaurian archosauriform clade Proterosuchidae, an extensive phylogenetic analysis posits X. sapingensis as a crown-group archosaur within Suchia, thus making this taxon the unequivocally oldest known member of Archosauria. The age and phylogenetic position of X. sapingensis indicate that many archosaurs, including all major clades of non-archosaurian archosauriforms, the avianline, ornithosuchids, aetosaurs and paracrocodylomorph lineages, must have diverged by the end of the Early Triassic. X. sapingensis is part of a possible clade of sail-backed poposauroids that were common components of archosaur assemblages during the Early to Middle Triassic.


Author(s):  
Cheng‐Yao Hou ◽  
Liu‐Hui Zhang ◽  
Yuan‐Hang Zhang ◽  
Jian‐Tao Cui ◽  
Li Zhao ◽  
...  

Land ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 471
Author(s):  
Bo Mu ◽  
Guohang Tian ◽  
Gengyu Xin ◽  
Miao Hu ◽  
Panpan Yang ◽  
...  

An understanding of the scientific layout of surface water space is crucial for the sustainable development of human society and the ecological environment. The objective of this study was to use land-use/land-cover data to identify the spatiotemporal dynamic change processes and the influencing factors over the past three decades in Henan Province, central China. Multidisciplinary theories (landscape ecology and graph theory) and methods (GIS spatial analysis and SPSS correlation analysis) were used to quantify the dynamic changes in surface water pattern and connectivity. Our results revealed that the water area decreased significantly during the periods of 1990–2000 and 2010–2018 due to a decrease in tidal flats and linear waters, but increased significantly in 2000–2010 due to an increase in patchy waters. Human construction activities, socioeconomic development and topography were the key factors driving the dynamics of water pattern and connectivity. The use of graph metrics (node degree, betweenness centrality, and delta probability of connectivity) in combination with landscape metrics (Euclidean nearest-neighbor distance) can help establish the parameters of threshold distance between connected habitats, identify hubs and stepping stones, and determine the relatively important water patches that require priority protection or development.


Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 1
Author(s):  
RUI-HONG WANG ◽  
MAO-QIN XIA ◽  
JIN-BO TAN ◽  
CHUAN CHEN ◽  
XIN-JIE JIN ◽  
...  

A new species, Scrophularia jinii (Scrophulariaceae), from Central China is described and illustrated. This new species was formerly misidentified as S. fargesii, from which it differs in many morphological characters. Moreover, it is distinct with all known Scrophularia species in its unique deeply double serrate leaf margin with 3–7 big teeth on each side. Molecular phylogenetic analysis further supports its species delimitation and suggests a close relationship with several Japanese and North American species.


2018 ◽  
Vol 254 ◽  
pp. 1-5 ◽  
Author(s):  
P. Díaz ◽  
E. Navarro ◽  
A. Prieto ◽  
A. Pérez-Creo ◽  
M. Viña ◽  
...  

2009 ◽  
Vol 46 (9) ◽  
pp. 651-661 ◽  
Author(s):  
Constanze Bickelmann ◽  
Johannes Müller ◽  
Robert R. Reisz

A restudy of the Upper Permian diapsid Acerosodontosaurus piveteaui from Madagascar indicates that the bone formerly identified as the quadratojugal is a fragment of a rib. This in turn implies that, in contrast to previous studies, the lower temporal arcade must be considered incomplete and derived relative to the ancestral condition. Since the phylogenetic position of Acerosodontosaurus is poorly understood, the taxon was entered into a modified phylogenetic data matrix of diapsid reptiles, and the purported monophyly of “Younginiformes” was tested for the first time by including all potential members of the clade as separate taxa, as well as other taxa from the same deposits. The results of the phylogenetic analysis do not support the monophyly of “younginiform” reptiles. Instead, most taxa cluster unresolved at the base of Neodiapsida, a finding that has important implications for the understanding of early diapsid evolution because it suggests that early neodiapsids represent several distinct evolutionary lineages. Acerosodontosaurus and Hovasaurus do form a clade, a finding consistent with the stratigraphic age and biogeography of these taxa.


PhytoKeys ◽  
2020 ◽  
Vol 156 ◽  
pp. 103-112
Author(s):  
Jiao Sun ◽  
Dai-Gui Zhang ◽  
Xian-Han Huang ◽  
Komiljon Tojibaev ◽  
Jing-Yuan Yang ◽  
...  

This report provides a description of Primula sunhangii from the Shennongjia Forestry District, Hubei Province in Central China, which is categorized as a new species of the primrose family. Primula sunhangii is morphologically similar to P. involucrata Wall. ex Duby in terms of its simple umbel, efarinose, and prolonged bracts. However, P. sunhangii is distinguished by its glabrous sepal, short petiole (compare with blade) and cylindrical calyx and capsule. Molecular phylogenetic analysis based on nuclear and cpDNA genes demonstrates that P. sunhangii and P. involucrata are closely related. Combining genetic and morphological data, the recognition of P. sunhangii as a unique new species is supported.


2018 ◽  
Author(s):  
Mingwei Cai ◽  
Yang Liu ◽  
Zhichao Zhou ◽  
Yuchun Yang ◽  
Jie Pan ◽  
...  

AbstractAsgard is a newly proposed archaeal superphylum. Phylogenetic position of Asgard archaea and its relationships to the origin of eukaryotes is attracting increasingly research interest. However, in-depth knowledge of their diversity, distribution, and activity of Asgard archaea remains limited. Here, we used phylogenetic analysis to cluster the publicly available Asgard archaeal 16S rRNA gene sequences into 13 subgroups, including five previously unknown subgroups. These lineages were widely distributed in anaerobic environments, with the majority of 16S rRNA gene sequences (92%) originating from sediment habitats. Co-occurrence analysis revealed potential relationships between Asgard, Bathyarchaeota, and Marine Benthic Group D archaea. Genomic analysis suggested that Asgard archaea are potentially mixotrophic microbes with divergent metabolic capabilities. Importantly, metatranscriptomics confirmed the versatile lifestyles of Lokiarchaeota and Thorarchaeota, which can fix CO2using the tetrahydromethanopterin Wood-Ljungdahl pathway, perform acetogenesis, and degrade organic matters. Overall, this study broadens the understandings of Asgard archaea ecology, and also provides the first evidence to support a transcriptionally active mixotrophic lifestyle of Asgard archaea, shedding light on the potential roles of these microorganisms in the global biogeochemical cycling.


1990 ◽  
Vol 38 (5) ◽  
pp. 519 ◽  
Author(s):  
PR Baverstock ◽  
M Krieg ◽  
J Birrell ◽  
GM Mckay

Microcomplement fixation of albumin was used to examine the phylogenetic relationships among the ringtail possums, family Pseudocheiridae. Phylogenetic analysis of the data supports the hypothesis of at least three distinct clades within the family: one containing Petauroides and Hemibelideus; a second consisting of Pseudocheirus herbertensis, Ps. forbesi, Ps. mayeri, and Ps. canescens; and a third containing Ps. archeri, Ps. corinnae, Ps. cupreus and Ps. dahli. The data have not resolved the phylogenetic position of Ps. peregrinus, which may either form a separate clade or lie close to the Ps. archeri clade.


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