Morphological and genetic description of Moniliformis necromysi sp. n. (Archiacanthocephala) from the wild rodent Necromys lasiurus (Cricetidae: Sigmondontinae) in Brazil

2020 ◽  
Vol 94 ◽  
Author(s):  
A.P.N. Gomes ◽  
N.A. Costa ◽  
R. Gentile ◽  
R.V. Vilela ◽  
A. Maldonado
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Genetic Distances ◽  
Brazilian Cerrado ◽  
Wild Rodent ◽  
The Family ◽  
Molecular Phylogenies ◽  
A New Species

Abstract A new species of Moniliformis Travassos, 1915 (Acanthocephala: Moniliformidae) is described from the hairy-tailed bolo mouse, Necromys lasiurus Lund, 1840 (Cricetidae: Sigmondontinae), captured in the Brazilian Cerrado, in Uberlândia, state of Minas Gerais, Brazil. The specimens were studied by light and scanning electron microscopy. Molecular phylogenies were inferred from partial nuclear large subunit ribosomal RNA gene sequences and partial mitochondrial cytochrome c oxidase subunit I gene. The new species is distinguished from other moniliformid species by the number of rows and number of hooks per row, size of the proboscis, size of the eggs, host species and geographical distribution. Molecular phylogenies and genetic distances analyses demonstrated that Moniliformis necromysi sp. n. forms a well-supported monophyletic group with sequences of other species of Moniliformis and is distinguished from them, which agrees with the morphological characteristics, allocating the new species to this genus and to the family Moniliformidae Van Cleave, 1924. This is the first moniliformid acanthocephalan described from a wild rodent in Brazil.

Ceriporia albomellea (Phanerochaetaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidences

Phytotaxa ◽  
2017 ◽  
Vol 298 (1) ◽  
pp. 20 ◽  
Author(s):  
YUAN YUAN ◽  
XIAO-HONG JI ◽  
FANG WU ◽  
JIA-JIA CHEN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Its Regions ◽  
Tropical China ◽  
A New Species

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.

A new species of Skeletocutis (Polyporales, Basidiomycota) from Yunnan of China

Phytotaxa ◽  
2016 ◽  
Vol 270 (4) ◽  
pp. 267 ◽  
Author(s):  
LU-SEN BIAN ◽  
CHANG-LIN ZHAO ◽  
FANG WU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Its Regions ◽  
A New Species

A new species of Polyporales, named as Skeletocutis yunnanensis, was collected on angiosperm wood in northern Yunnan Province, southwestern China. It is described based on morphological characteristics and molecular evidence. The species belongs to the Skeletocutis subincarnata complex, but differs morphologically from all known species of the genus by white, cream to buff pores surface, angular pores mostly 5–6 per mm with entire mouths, a dimitic hyphal structure both in trama and subiculum, generative hyphae in whole basidiocarps covered by fine crystals, skeletal hyphae unchanged in KOH, not agglutinated, allantoid basidiospores measured as 3.5–4.5 × 1.0–1.2 µm, and growth on angiosperm wood. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions indicated that the new species grouped with Skeletocutis and nested in the tyromyces clade.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Morphological and phylogenetic analyses reveal a new species of Fistulina (Fistulinaceae, Agaricales) from Australia

Phytotaxa ◽  
2019 ◽  
Vol 420 (3) ◽  
pp. 233-240
Author(s):  
YI-FEI SUN ◽  
SHUN LIU ◽  
BAO-KAI CUI
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Pore Surface ◽  
Ribosomal Rna Gene ◽  
Hyphal System ◽  
Cotton Blue ◽  
A New Species

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 401 (3) ◽  
pp. 199
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Lecanicillium Aphanocladii – A New Species to the Mycoflora of Lithuania and a New Pathogen of Tree Leaves Mining Insects

2012 ◽  
Vol 18 (2) ◽  
pp. 133-146 ◽  
Author(s):  
Dalė Pečiulytė ◽  
Audrius Kačergius
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Morphological Characteristics ◽  
Horse Chestnut ◽  
Tilia Cordata ◽  
Tree Leaves ◽  
Leaf Mining ◽  
Phyllonorycter Issikii ◽  
Recovery Percentage ◽  
A New Species

Abstract Pečiulytė D., Kačergius A., 2012: Lecanicillium aphanocladii - a new species to the mycoflora of Lithuania and a new pathogen of tree leaves mining insects [Lecanicillium aphanocladii - nauja Lietuvos mikofloros rūšis ir naujas medžių lapus minuojančių vabzdžių patogenas pasaulyje]. - Bot. Lith., 18(2); 133-146. Popular park and city trees horse chestnut, Aesculus hippocastanum L. and small-leaved linden, Tilia cordata Miller suffer from damages of leaf mining moths, Cameraria ohridella Deschka & Dimic 1968 and Lithocolletis issikii Kumata 1825 (= Phyllonorycter issikii Humber 1822 (both Lepidoptera, Gracillariidae), respectively. In 2010-2011, fungi causing moth caterpillar mortality under field conditions were investigated in Vilnius city, Lithuania. Statistical analyses showed high recovery percentage (30.36 % and 31.92 % from C. ohridella and L. issikii caterpillar cadavers, respectively) of a new fungus, which was thereafter identified as Lecanicillium aphanocladii Zare & W. Gams 2001 based on a morphological characteristics and ITS rDNR sequence, which showed 99 % identity with Aphanocladium aranearum (Petch) W. Gams 1971 GenBank accessions No. AF455489 and AF455405 of ribosomal RNA gene (now renamed as L. aphanockladii).

scholarly journals Multi-Gene Phylogeny and Taxonomy of Hydnellum (Bankeraceae, Basidiomycota) from China

2021 ◽  
Vol 7 (10) ◽  
pp. 818
Author(s):  
Yan-Hong Mu ◽  
Jia-Rui Yu ◽  
Ting Cao ◽  
Xiang-Hua Wang ◽  
Hai-Sheng Yuan
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Ribosomal Rna Gene ◽  
Morphological Studies

The genus Hydnellum is an important group of stipitate hydnaceous fungi which can form ectomycorrhiza with many species of woody plants. In recent decades, the frequency and number of basidiocarps observed in China have been declining significantly. So far, however, we know little about the species diversity of Hydnellum in China. In this study, we conducted molecular phylogenetic analyses based on sections of multiple loci, including the large subunit of nuclear ribosomal RNA gene (nLSU), the internal transcribed spacer regions (ITS), the small subunit of nuclear ribosomal RNA gene (SSU) and the second-largest subunit of RNA polymerase II gene (RPB2), as well as morphological studies, of collected samples of Hydnellum from China. We also inferred Maximum Likelihood and Bayesian phylogenies for the order Thelephorales from the dataset of the combined nLSU and ITS. This study has revealed the phylogenetic position of Hydnellum in the order Thelephorales, and phylogenetically confirmed ten major clades in Thelephorales; Twenty-nine taxa are proposed, described or reported, including 10 new subgenera (Hydnellum subgenus Hydnellum, subg. Caesispinosum, subg. Croceum, subg. Inflatum, subg. Rhizomorphum, subg. Scabrosum, subg. Spongiosum, subg. Subindufibulatum, subg. Violaceum and subg. Zonatum), 11 new species (Hydnellum atrorubrum, H. atrospinosum, H. bomiense, H. brunneorubrum, H. fibulatum, H. granulosum, H. inflatum, H. rubidofuscum, H. squamulosum, H. sulcatum and H. yunnanense), 3 newly recorded species (H. caeruleum, H. peckii and H. spongiosipes) and 5 notable specimens (Hydnellum sp 1, H. sp 2, H. sp 3, H. sp 4 and H. sp 5). A classification system based on the morphological characteristics (especially the hyphal structure types) and molecular analyses is proposed to accommodate most species in Hydnellum. The distinguishing characters of the subgenera and the new species with their closely related taxa are discussed. A key to the species of Hydnellum from China is provided.

A new species of bonefish, Albula koreana (Albuliformes: Albulidae) from Korea and Taiwan

Zootaxa ◽  
2011 ◽  
Vol 2903 (1) ◽  
pp. 57 ◽  
Author(s):  
HYUCK JOON KWUN ◽  
JIN KOO KIM
Keyword(s):  
New Species ◽  
Gene Sequence ◽  
Genetic Distances ◽  
Similar Species ◽  
Base Pairs ◽  
Mitochondrial Cytochrome B ◽  
The Family ◽  
Mitochondrial Cytochrome B Gene ◽  
A New Species ◽  
Scientific Name

A new species of the genus Albula of the family Albulidae is described based on five specimens collected from Korea and Taiwan. Its new scientific name, Albula koreana sp. nov., is derived from its type locality (Korea). We compared Albula koreana sp. nov. with its similar species, A. argentea from Fiji (seven specimens), using morphological and molecular methods. Albula koreana sp. nov. differs morphologically from A. argentea in its tooth patch distributions on the mesopterygoids and parasphenoid. The tooth patches on the mesopterygoids are distributed more anteriorly than those on the parasphenoid in Albula koreana sp. nov. On the other hand, the anterior end of the tooth patches on the mesopterygoids almost corresponds to that of the tooth patches on the parasphenoid in A. argentea. The numbers of vertebrae also differ between the two species (77–78 in Albula koreana sp. nov. vs 72–73 in A. argentea). We analyzed 546 base pairs of the mitochondrial cytochrome b gene sequence, and the Albula koreana sp. nov. sequence differed considerably from that of A. argentea. Kimura’s genetic distances between them were very large (15.9%–16.4%), robustly supporting the new species Albula koreana sp. nov.

Morphological characteristics and phylogenetic analyses revealed a new Calocybe (Lyophyllaceae, Basidiomycota) species from northeast China

Phytotaxa ◽  
2021 ◽  
Vol 490 (2) ◽  
pp. 203-210
Author(s):  
JIZE XU ◽  
XIAODONG YU ◽  
CHUNLAN ZHANG ◽  
YU LI
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Northeast China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Molecular Phylogenetic ◽  
A New Species

A new species, Calocybe decurrens, is illustrated and described in detail based on morphological characteristics and phylogenetic analyses. Calocybe decurrens is mainly characterized by its decurrent gills and by its stipe that discolors upon maturation. Molecular phylogenetic analyses were based on the internal transcribed spacer (ITS1-5.8S-ITS2) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequences. The results indicated that its affiliation is in genus Calocybe, where it occupies an isolated position. A full description, color images, illustrations and a phylogenetic tree to show the placement of the new species are provided.

Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand

Phytotaxa ◽  
2020 ◽  
Vol 437 (1) ◽  
pp. 14-22
Author(s):  
YUAN S. LIU ◽  
JIAN-KUI (JACK) LIU ◽  
PHONGEUN SYSOUPHANTHONG ◽  
KEVIN D. HYDE ◽  
SAISAMORN LUMYONG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Molecular Evidence ◽  
Internal Transcribed Spacer Region ◽  
Northern Thailand ◽  
A New Species

Xanthagaricus siamensis, a new species in the family Agaricaceae, was discovered in northern Thailand and is here introduced based on its morphological features and molecular data. It is characterized by small to medium-sized basidiomata, a convex to plano-convex with depressed center pileus when mature, the presence of greyish orange to violet-brown fibrillose squamules on the pileus, lamellae that start out white, change to pinkish white when damaged, then change to dull green with age. Additionally, the annulus is fugacious, and the pileipellis is recognized as a cutis which morphologically distinguishes it from all other known Xanthagaricus. Phylogenetic analysis of the internal transcribed spacer region (ITS) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequence data indicated that the three specimens of X. siamensis form a distinct lineage within Xanthagaricus, and they formed a well-supported clade representing the genus Xanthagaricus. Detailed illustrations of macro- and micro-morphological characteristics and descriptions are provided, as well as other relevant molecular evidence.

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