Phenotypic variation in a significant spore character in Kudoa (Myxosporea: Multivalvulida) species infecting brain tissue

Parasitology ◽  
2010 ◽  
Vol 137 (12) ◽  
pp. 1759-1772 ◽  
Author(s):  
MIEKE A. A. BURGER ◽  
ROBERT D. ADLARD
Keyword(s):  
Brain Tissue ◽  
Principal Components ◽  
Sequence Data ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Pathogenic Species ◽  
Regression Analyses ◽  
Morphometric Data ◽  
Rdna Sequence Data ◽  
Morphological Differences

SUMMARYSome Kudoa species display variations in the number of polar capsules in spores within an individual pseudocyst. Nonetheless, there is usually a dominant morphotype which forms a significant element of diagnosis. In 2007, a Kudoa isolate from whiting (spores with 5 (dominant) or 6 (minor) polar capsules) was characterized by Burger et al. (2007) as being 100% identical in SSU rDNA to Kudoa yasunagai (spores with 7 polar capsules) from a halibut, despite its obvious morphological differences. The authors hypothesized that either SSU rDNA had reached its level of resolution or that the genetic identity revealed conspecificity. To further investigate these hypotheses, SSU and LSU rDNA sequence data were coupled with principal components, correlation, and regression analyses of morphometric data from different kudoid isolates that infect brain tissue to determine the relationships between spore morphotypes and different kudoid isolates. The trends in morphometrics between the spores of particular isolates were so similar that it was concluded that the molecular results did indicate conspecificity rather than SSU reaching its level of resolution. This phenotypic influence on a significant diagnostic character within the Kudoidae has a major impact on the diagnosis of this, and potentially other, pathogenic species.

scholarly journals Reconsideration of the ‘well-known’ hypotrichous ciliate Pleurotricha curdsi (Shi et al., 2002) Gupta et al., 2003 (Ciliophora, Sporadotrichida), with notes on its morphology, morphogenesis and molecular phylogeny

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3216-3225 ◽  
Author(s):  
Xiaoteng Lu ◽  
Chen Shao ◽  
Yuhe Yu ◽  
Alan Warren ◽  
Jie Huang
Keyword(s):  
Sequence Data ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Large Body ◽  
Small Subunit ◽  
Variable Number ◽  
Ssu Rdna ◽  
Intermediate Form ◽  
Rdna Sequence Data

The oxytrichid species Pleurotricha curdsi (Shi et al., 2002) Gupta et al., 2003, isolated from a tributary of the Yangtze River in the Mudong district of Chongqing, southern China, was reinvestigated with emphasis on its morphology, morphogenesis and small-subunit (SSU) rDNA-based phylogeny. Compared with three previously described populations, the Mudong population of P. curdsi is characterized by its large body size, 170–295 × 65–110 μm in vivo, and by having a variable number of right marginal rows, either two or three. Likewise, the number of right marginal rows anlagen (RMA) is also variable, i.e. usually two, but sometimes several small extra anlagen that give rise to the formation of the third row, are present to the left of the RMAs. We posit that the Mudong population is an intermediate form between the three previously described populations. Phylogenetic analyses based on the SSU rDNA sequence data show that all populations of P. curdsi cluster with the type species of the genus, Pleurotricha lanceolata, in a clade nested within the Oxytrichidae.

scholarly journals Redescription, Morphogenesis, and Molecular Phylogeny of Pseudosincirra longicirrata nov. comb., With Establishment of a New Genus Pseudosincirra nov. gen. (Ciliophora, Hypotrichia)

2021 ◽  
Vol 12 ◽  
Author(s):  
Qi Gao ◽  
Chen Shao ◽  
Qiuyue Tang ◽  
Jingbao Li
Keyword(s):  
Molecular Phylogeny ◽  
New Genus ◽  
Sequence Data ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Original Population ◽  
The Family ◽  
Rdna Sequence Data

The morphology and morphogenesis of Pseudosincirra longicirrata nov. gen. and nov. comb., isolated from southern China, were investigated with living observation and protargol staining. Our population is similar to the original population in living characteristics and ciliary patterns. The main determinable morphogenetic features of P. longicirrata nov. comb. are the presence of five frontoventral-transverse cirral anlagen (FVT-anlagen) and a dorsomarginal kinety anlage. According to the origin of FVT-anlagen IV and V in proter, it can be determined that P. longicirrata nov. comb. possesses two frontoventral rows and one right marginal row. Hence, a new genus, Pseudosincirra nov. gen., is proposed, and the diagnosis of P. longicirrata nov. comb. is improved. The new genus is diagnosed as follows: adoral zone of membranelles and undulating membranes is in a Gonostomum pattern; there are three enlarged frontal cirri, one buccal cirrus, and one parabuccal cirrus; postperistomial cirrus and transverse cirri are lacking; there are two more or less long frontoventral rows and one right and two or more left marginal rows; cirri within all rows very widely spaced; dorsal kinety pattern is of Urosomoida type, that is, three dorsal kineties and one dorsomarginal kinety; and caudal cirri are present. Phylogenetic analyses based on the small subunit ribosomal (SSU rDNA) sequence data indicate that P. longicirrata nov. comb. clusters with Deviata and Perisincirra. It is considered that Pseudosincirra nov. gen. and Perisincirra paucicirrata should be assigned to the family Deviatidae; fine cirri, and cirri within all rows being relatively widely spaced, should be considered as plesiomorphies of Deviatidae; and Deviatidae is closely related to Dorsomarginalia or Strongylidium–Hemiamphisiella–Pseudouroleptus.

PSEUDULVELLA AMERICANABELONGS TO THE ORDER CHAETOPELTIDALES (CLASS CHLOROPHYCEAE), EVIDENCE FROM ULTRASTRUCTURE AND SSU RDNA SEQUENCE DATA

2006 ◽  
Vol 42 (4) ◽  
pp. 943-950 ◽  
Author(s):  
M. Virginia Sanchez-Puerta ◽  
Patricia I. Leonardi ◽  
Charles J. O'Kelly ◽  
Eduardo J. Cáceres
Keyword(s):  
Sequence Data ◽  
Ssu Rdna ◽  
Rdna Sequence ◽  
Rdna Sequence Data

Eucheumatopsis sanibelensis sp. nov. from the Gulf coast of Florida, USA

Phytotaxa ◽  
2020 ◽  
Vol 440 (3) ◽  
pp. 215-224 ◽  
Author(s):  
ELIAS S. PETERSON ◽  
CRAIG W. SCHNEIDER ◽  
GARY W. SAUNDERS
Keyword(s):  
Gulf Coast ◽  
Sequence Data ◽  
Algal Species ◽  
Morphological Characteristics ◽  
Caribbean Sea ◽  
Lsu Rdna ◽  
Eastern Gulf Of Mexico ◽  
Single Location ◽  
Red Algal ◽  
Morphological Differences

Based upon COI-5P, LSU rDNA and rbcL sequence data, as well as its morphological characteristics, a new red algal species, Eucheumatopsis sanibelensis E.S.Peterson, C.W.Schneider et G.W.Saunders sp. nov., was discovered on the Gulf coast of Florida, USA and shown to be distinct from the generitype E. isiformis with a type locality in the eastern Caribbean Sea. The new more attenuate taxon from Sanibel Island represents only the second species in this newly described genus, a genus recently segregated from Eucheuma based upon significant molecular and morphological differences. The two species have overlapping geographic distributions in the eastern Gulf of Mexico, but thus far E. sanibelensis is only known from a single location.

Generic delimitations in the family Stictidaceae (Ostropales, Ascomycota): the Stictis–Conotrema problem

2005 ◽  
Vol 37 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Mats WEDIN ◽  
Heidi DÖRING ◽  
Kristina KÖNBERG ◽  
Gunnar GILENSTAM
Keyword(s):  
Sequence Data ◽  
Sister Group ◽  
Lsu Rdna ◽  
Infrageneric Classification ◽  
Rdna Its ◽  
The Family ◽  
Rdna Sequence Data ◽  
Natural Groups ◽  
High Support

The family Stictidaceae (Ostropales, Ascomycota) contains both lichenized and non-lichenized fungi. Here, we test if Conotrema (lichenized) and Stictis (non-lichenized) as currently delimited are distinct monophyletic genera, by parsimony and parsimony jackknifing analyses of combined nuclear rDNA (ITS and partial LSU rDNA) and mitochondrial SSU rDNA sequence data matrices. The study includes four species of Stictis, three species of Conotrema, and representatives of the related Schizoxylon (lichenized), Odontotrema, Carestiella (at least sometimes associated with algae), Cryptodiscus and Thelotrema (lichenized). In all analyses, the Conotrema species were nested within Stictis with high support. Thus, we conclude that Conotrema are only lichenized representatives of Stictis. The type species of the two generic names, C. urceolatum and S. radiata, are sister taxa in our analyses. Furthermore, the analysis gave no support for the present infrageneric classification of Stictis. Carestiella socia (the type of Carestiella) and the two representatives of Schizoxylon studied were also nested within Stictis s. lat. The Odontotremataceae is the sister group to the Stictidaceae, and Cryptodiscus foveolaris groups with Thelotrema rather than with the Stictidaceae. We conclude that lichenization in the Stictidaceae does not characterize natural groups, and that Conotrema should be considered a synonym to Stictis, as predicted by anatomical characteristics. The new combinations Stictis urceolatum and Stictis populorum are made.

Molecular systematics of Craterellus: cladistic analysis of nuclear LSU rDNA sequence data

2000 ◽  
Vol 104 (4) ◽  
pp. 388-394 ◽  
Author(s):  
Mattias Dahlman ◽  
Eric Danell ◽  
Joseph W. Spatafora
Keyword(s):  
Molecular Systematics ◽  
Sequence Data ◽  
Cladistic Analysis ◽  
Rdna Sequence ◽  
Lsu Rdna ◽  
Rdna Sequence Data

Phylogeny of the genus Bryoria

2011 ◽  
Vol 43 (6) ◽  
pp. 617-638 ◽  
Author(s):  
Leena MYLLYS ◽  
Saara VELMALA ◽  
Håkon HOLIEN ◽  
Pekka HALONEN ◽  
Li-Song WANG ◽  
...  
Keyword(s):  
Growth Form ◽  
Sequence Data ◽  
Molecular Study ◽  
Morphological Characters ◽  
Ssu Rdna ◽  
Species Status ◽  
Infrageneric Classification ◽  
Rdna Sequence Data ◽  
Cladistic Analyses ◽  
Current Species

AbstractThe phylogenetic relationships of the genus Bryoria were examined using ITS, partial glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and mitochondrial SSU rDNA sequence data in addition to 20 chemical and morphological characters. This first comprehensive molecular study to assess Bryoria phylogeny includes representatives from all the traditionally recognized four sections. Combined cladistic analyses of 88 Bryoria specimens representing at least 25 species resulted in highly resolved phylogenies. Based on the results, a new infrageneric classification for the genus is proposed. Five sections are recognized, largely corresponding to the existing classification, with the addition of section Americanae. The study shows that while most species with an erect growth-form are clearly monophyletic, current species status of many pendent taxa can be questioned.

scholarly journals Diversity and distribution of Blastocystis sp. subtypes in non-human primates

Parasitology ◽  
2013 ◽  
Vol 140 (8) ◽  
pp. 966-971 ◽  
Author(s):  
MOHAMMED A. ALFELLANI ◽  
ALISON S. JACOB ◽  
NATALI ORTIZ PEREA ◽  
ROSINA C. KRECEK ◽  
DERYA TANER-MULLA ◽  
...  
Keyword(s):  
Host Specificity ◽  
New World ◽  
Sequence Data ◽  
Ssu Rdna ◽  
Restricted Range ◽  
Free Living ◽  
Allele Distribution ◽  
Rdna Sequence Data ◽  
Blastocystis Sp ◽  
Geographical Association

SUMMARYBlastocystis SSU-rDNA sequence data from 317 captive and free-living non-human primates (NHPs) representing 30 genera of apes, Old and New World (OW and NW) monkeys and prosimians were analysed to investigate subtype (ST) and allele distribution among hosts. Excluding 20 mixed ST infections, 27% of the sequences belonged to ST1, 22% to ST2, 34% to ST3, 1% to ST4, 4% to ST5, 11% to ST8, <1% to ST13 and 1% to ST15. The study confirmed cryptic host specificity of ST1 and ST3; conversely, considerable overlap in ST2 alleles exists among humans and NHPs. Subtype distribution in humans and NHPs differs mainly in that ST4 is rarely reported in NHPs while ST5 and ST8 are both unusual in humans. This may be due to host specificity and/or the apparent geographically restricted range of some subtypes. While the distribution of ST1, ST2 and ST3 was independent of NHP group or geographical association, ST5 was seen only in apes and OW monkeys and ST8 primarily in arboreal NHPs and only in species native to Asia or South America.

Sign in / Sign up

Export Citation Format

Share Document