scholarly journals A review of sarcocystosis in camels and redescription of Sarcocystis cameli and Sarcocystis ippeni sarcocysts from the one-humped camel (Camelus dromedarius)

Parasitology ◽  
2015 ◽  
Vol 142 (12) ◽  
pp. 1481-1492 ◽  
Author(s):  
J. P. DUBEY ◽  
M. HILALI ◽  
E. VAN WILPE ◽  
R. CALERO-BERNAL ◽  
S. K. VERMA ◽  
...  

SUMMARYThere is considerable confusion concerning Sarcocystis species in camels. Five species: Sarcocystis cameli, Sarcocystis ippeni, Sarcocystis camelicanis, Sarcocystis camelocanis and Sarcocystis miescheri were named with inadequate descriptions and no type specimens. Here, we review literature on sarcocystosis in camels worldwide and redescribe structure of S. cameli and S. ippeni sarcocysts by light- and transmission electron microscopy (LM and TEM). Eight sarcocysts from the oesophagi of two camels (Camelus dromedarius) from Egypt were studied. By LM, all sarcocysts were thin-walled with barely visible projections on the cyst walls. By TEM, two structurally distinct sarcocysts were recognized by unique villar protrusions (vp) not found in sarcocysts from any other host. Sarcocysts of S. cameli had vp of type 9j. The sarcocyst wall had upright slender vp, up to 3·0 µm long and 0·5 µm wide; the total thickness of the sarcocyst wall with ground substance (gs) layer was 3·5 µm. On each vp, there were rows of knob-like protrusions that appeared to be interconnected. The vp had microtubules that originated at midpoint of the gs and continued up to the tip; microtubules were smooth, without any granules or dense areas. Bradyzoites were approximately 14–15 × 3–4 µm in size with typical organelles. Sarcocystis ippeni sarcocysts had type 32 sarcocyst wall characterized by conical vp with an electron dense knob. The total thickness of the sarcocyst wall (from the base of gs to vp tip) was 2·3–3·0 µm. The vp were up to 1·2 µm wide at the base and 0·25 µm at the tip. Microtubules in vp originated at midpoint of gs and continued up to tip; microtubules were criss-crossed, smooth and without granules or dense areas. Bradyzoites were 12·0–13·5 × 2·0–3·0 µm in size. Sarcocystis camelicanis, S. camelocanis and S. miescheri are considered invalid.

Parasitology ◽  
2015 ◽  
Vol 143 (1) ◽  
pp. 18-23 ◽  
Author(s):  
J. P. DUBEY ◽  
E. VAN WILPE ◽  
S. K. VERMA ◽  
M. HILALI

SUMMARYThere is considerable confusion concerning Sarcocystis species in equids. Little is known of Sarcocystis infections in donkeys (Equus asinus). Here we describe the structure of Sarcocystis bertrami-like from the donkey by light microscopy (LM) and transmission electron microscopy (TEM). Nineteen sarcocysts from the tongue of a donkey from Egypt were studied both by LM and TEM. By LM, all sarcocysts had variably shaped and sized projections on the sarcocyst walls, giving it a thin-walled to thick-walled appearance, depending on individual sarcocyst and plane of section. By TEM, sarcocysts walls had villar protrusions (vp) of type 11. The sarcocyst wall had conical to slender vp, up to 6 µm long and 1 µm wide; the vp were folded over the sarcocyst wall. The total thickness of the sarcocyst wall with ground substance layer (gs) was 1–3 µm. The vp had microtubules (mt) that originated deeper in the gs and continued up to the tip. The apical part of the vp had electron dense granules. The mt were configured into 3 types: a tuft of electron dense mt1 extending the entire length of the vp with a tuft of medium electron dense mt2 appearing in parallel, and fine mt3 present only in the villar tips. The gs was mainly smooth with few indistinct granules. All sarcocysts were mature and contained metrocytes and bradyzoites. Bradyzoites were approximately 11–15 × 2–3 µm in size with typical organelles.


Author(s):  
P. R. Swann ◽  
W. R. Duff ◽  
R. M. Fisher

Recently we have investigated the phase equilibria and antiphase domain structures of Fe-Al alloys containing from 18 to 50 at.% Al by transmission electron microscopy and Mössbauer techniques. This study has revealed that none of the published phase diagrams are correct, although the one proposed by Rimlinger agrees most closely with our results to be published separately. In this paper observations by transmission electron microscopy relating to the nucleation of disorder in Fe-24% Al will be described. Figure 1 shows the structure after heating this alloy to 776.6°C and quenching. The white areas are B2 micro-domains corresponding to regions of disorder which form at the annealing temperature and re-order during the quench. By examining specimens heated in a temperature gradient of 2°C/cm it is possible to determine the effect of temperature on the disordering reaction very precisely. It was found that disorder begins at existing antiphase domain boundaries but that at a slightly higher temperature (1°C) it also occurs by homogeneous nucleation within the domains. A small (∼ .01°C) further increase in temperature caused these micro-domains to completely fill the specimen.


Author(s):  
C. C. Ahn ◽  
S. Karnes ◽  
M. Lvovsky ◽  
C. M. Garland ◽  
H. A. Atwater ◽  
...  

The bane of CCD imaging systems for transmission electron microscopy at intermediate and high voltages has been their relatively poor modulation transfer function (MTF), or line pair resolution. The problem originates primarily with the phosphor screen. On the one hand, screens should be thick so that as many incident electrons as possible are converted to photons, yielding a high detective quantum efficiency(DQE). The MTF diminishes as a function of scintillator thickness however, and to some extent as a function of fluorescence within the scintillator substrates. Fan has noted that the use of a thin layer of phosphor beneath a self supporting 2μ, thick Al substrate might provide the most appropriate compromise for high DQE and MTF in transmission electron microcscopes which operate at higher voltages. Monte Carlo simulations of high energy electron trajectories reveal that only little beam broadening occurs within this thickness of Al film. Consequently, the MTF is limited predominantly by broadening within the thin phosphor underlayer. There are difficulties however, in the practical implementation of this design, associated mostly with the mechanical stability of the Al support film.


Acta Tropica ◽  
2020 ◽  
Vol 207 ◽  
pp. 105500
Author(s):  
Walaa Mohammed Abdelazeem ◽  
Tara Rava Zolnikov ◽  
Zeinab Roshdy Mohammed ◽  
Alaa Saad ◽  
Kamelia M Osman

2014 ◽  
Vol 43 (2) ◽  
pp. 218-223 ◽  
Author(s):  
Diego Blanco ◽  
José M. Vázquez ◽  
Miguel A. Rivero ◽  
Juan A. Corbera ◽  
Alberto Arencibia

2016 ◽  
Vol 33 (04) ◽  
pp. 171-178
Author(s):  
Z. Adam ◽  
A. Awaad ◽  
M. Tawfiek ◽  
A. Ibrahim

Abstract Introduction: The objective of this study was to clarify the anatomy of the narial and labial musculatures of the one-humped camel (Camelus dromedarius) and their nerve supply. Materials and Methods: Sixteen head specimens from adult and symptomatically healthy camels of both sexes were used. The muscles of the nostrils and lips were carefully dissected and illustrated to demonstrate their origin, insertion and relations. The nerves in this area were also dissected to show their branches and distribution. Results: The dissection of these regions revealed that their muscles were arranged in three layers; the superficial layer included M. dilator naris apicalis, M. dilator naris medialis and M. levator nasolabialis, the middle layer was formed of maxillo-labial group of muscles (M. levator labii superioris, M. dilator naris lateralis and M. depressor labii superioris) and the deep layer was formed by M. lateralis nasi. Moreover, the lips had M. orbicularis oris, M. incisivus superioris, M. incisivus inferioris and M. mentalis, however, the M. depressor labii inferioris was absent in the animal under investigation. The muscles of nostrils and lips were innervated by N. trigeminus (V) and N. facialis (VII). Conclusion: The arrangement of the narial and labial muscles is unique and may relate to its living conditions of frequent sand-storms and direct sun rays, where the camel is the only domesticated animal known for its ability to close its nostril.


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