An outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia

Parasitology ◽  
2016 ◽  
Vol 144 (6) ◽  
pp. 738-746 ◽  
Author(s):  
HAGOS GEBREKIDAN ◽  
LINDA NELSON ◽  
GREG SMITH ◽  
ROBIN B. GASSER ◽  
ABDUL JABBAR
Keyword(s):  
Dairy Cattle ◽  
Phylogenetic Analyses ◽  
Surface Protein ◽  
Theileria Orientalis ◽  
Group 3 ◽  
Cattle Blood ◽  
The Difference ◽  
Group 2 ◽  
Major Piroplasm Surface Protein ◽  
Group 1

SUMMARYThis study reports an outbreak of oriental theileriosis in dairy cattle imported to Vietnam from Australia. Following clinical and pathological diagnoses, a total of 112 cattle blood samples were divided into three groups and tested using multiplexed tandem PCR. Group 1 were from aborted heifers in Vietnam; group 2 were from cattle before shipment from group 1 cattle and group 3 were from the same batch of cattle but transported to Taiwan. Theileria orientalis DNA was detected in 72·3% cattle. The prevalences of T. orientalis in groups 1, 2 and 3 were 77·6, 86·9 and 57·5%, respectively, and the difference in prevalence was significant between groups 1 and 3 (P < 0·0001). The infection intensities of genotypes chitose and ikeda of T. orientalis were higher in groups 1 (57 721 and 33 709, respectively) and 3 (5897 and 61 766, respectively) than those in group 2 (2071 and 6331, respectively). Phylogenetic analyses of the major piroplasm surface protein sequences revealed that genotypes chitose and ikeda determined herein were closely related to those previously reported from Australia. This first report of an outbreak of oriental theileriosis in imported cattle emphasizes improved measures for the export and import of cattle infected with T. orientalis.

scholarly journals The Effectiveness of Endoscopic Surgery of Juvenile Angiofibroma Using Surgical Glue

2020 ◽  
Vol 8 (B) ◽  
pp. 150-154
Author(s):  
Seyed-Hadyi Samimi Ardesan ◽  
Mojtaba Mohammadi Ardehali ◽  
Najmeh Doustmohammadian
Keyword(s):  
Blood Loss ◽  
Endoscopic Surgery ◽  
Tumor Staging ◽  
Low Cost ◽  
Tumor Stage ◽  
Nasopharyngeal Angiofibroma ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

AIM: The current study aimed to provide a method for juvenile nasopharyngeal angiofibroma embolization using Glubran glue in patients with low stage tumor. This method not only has less blood loss and good visualization but also impose a low cost, where no pre-operative embolization complications were found for this procedure. METHODS: Between 2012 and 2014, 30 patients with angiofibroma undergoing endoscopic surgery. Age, sex, tumor stage, average blood loss, complications, length of hospitalization, and recurrence rate of the tumor were the main measured outcomes. Furthermore, 30 patients were divided into three groups with matched age, sex, and tumor staging. Group 1 received glue (Glubran), while Group 2 selected for study without glue and embolization and pre-operative embolization was considered for Group 3. RESULTS: Based on the amount bleeding, the mean blood hemorrhage in Groups 1, 2, and 3 was 510, 1655, and 800 ml, respectively, the difference of hemorrhage between Groups 1 and 2 was found to be statistically significant (p = 0.007). Blood loss in Group 1 was found to be less than Group 3, but the difference of hemorrhage between Group 1 and 3 was not statistically significant (p = 0.678). No blood transfusion and complication were recorded for individuals in Group 1. The recurrence was found in 1 patient (10%) in both groups of 2 and 3, and no patient (0%) in Group 1. CONCLUSIONS: The direct intraoperative embolization technique with glue was capable of providing a more complete and targeted embolization of the tumor. Some advantages can be mentioned for this technique, including decreased blood loss, less radiation exposure, lower rates of complications, and recurrence, as well as shorter hospitalization time, the ease of procedure with a spinal needle and low cost.

scholarly journals Plasma as an indicator of bone fluoride levels in rats chronically exposed to fluoride

2006 ◽  
Vol 14 (4) ◽  
pp. 238-241 ◽  
Author(s):  
Juliane Guimarães de Carvalho ◽  
Rodrigo Cardoso de Oliveira ◽  
Marília Afonso Rabelo Buzalaf
Keyword(s):  
Wistar Rats ◽  
Direct Method ◽  
Facilitated Diffusion ◽  
Bone Surface ◽  
Male Wistar Rats ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Exposure Methods ◽  
Group 1

OBJECTIVE: This study evaluated the use of plasma, bone surface (periosteal) and whole bone as biomarkers of chronic fluoride (F) exposure. METHODS: Forty male Wistar rats were assigned to 4 groups (n=10/gr) that differed according to the F concentration they received in the drinking water. Groups 1, 2, 3 and 4 received water containing 0 (control), 5, 15, and 50 mg F/L, respectively. The rats were killed at 120 days of age. Plasma and femur were collected and analyzed for fluoride with the ion specific electrode by the direct method or after hexamethyldisiloxane-facilitated diffusion. Data were tested for statistically significant differences by ANOVA and linear regression (p<0.05). RESULTS: Mean (± SE) plasma F concentrations ranged from 0.030 ± 0.002 to 0.187 ± 0.013 (mg/mL). The concentrations in surface and whole bone ranged from 610 ± 32 to 4,693 222; and 647 ± 22 to 3,439 ± 134 µg/g, respectively. The surface/whole F concentration ratios were 0.941, 1.414, 1.173 and 1.377, for groups 1, 2, 3 and 4 respectively. For plasma and whole bone, the difference among all groups was statistically significant, except for group 2 compared to group 1. For bone surface, all groups differed from each other except for group 2 compared to group 3. A significant positive correlation was found between bone surface and whole bone F (r²=0.94), as well as between plasma and bone surface (r²=0.71) and plasma and whole bone (r²=0.74). CONCLUSIONS: Data suggest that both bone surface and whole bone are suitable biomarkers of chronic F exposure in rats and plasma may be used as indicator of bone fluoride levels.

scholarly journals Evaluation of the Clinical Efficacy of Using Thermal Camera for Cryotherapy in Patients with Total Knee Arthroplasty: A Prospective Study

Medicina ◽  
2019 ◽  
Vol 55 (10) ◽  
pp. 661 ◽  
Author(s):  
Zekeriya Okan Karaduman ◽  
Ozan Turhal ◽  
Yalçın Turhan ◽  
Zafer Orhan ◽  
Mehmet Arican ◽  
...  
Keyword(s):  
Total Knee Arthroplasty ◽  
Knee Arthroplasty ◽  
Postoperative Period ◽  
Thermal Camera ◽  
Prosthesis Infection ◽  
Total Knee ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

Background and objectives: Cryotherapy is a method of treatment using cold application. This study aimed to evaluate postoperative clinical and hematological parameters and pain associated with total knee arthroplasty in patients and compared cryotherapy to the conventional method of cold ice pack compressions. Materials and Methods: Between January 2015 and January 2016, 90 patients who underwent total knee arthroplasty for grade 4 gonarthrosis were prospectively evaluated. The patients were divided into three groups (n = 30, each): Group 1, cryotherapy was applied in the pre- and postoperative periods; Group 2, cryotherapy was applied only in the postoperative period; and Group 3 (control group), only a cold pack (gel ice) was applied postoperatively. In all groups, pre- and postoperative evaluations at 6, 24, and 48 h, hemorrhage follow-up, knee circumference measurement, visual analog scale pain score, knee circumference, and temperature measured by thermal camera were recorded. Results: Of the 90 patients, 10% were men and 90% were women. The mean age was 64.3 ± 8.1 (range: 46–83) years. The patella upper end diameter values were significantly lower in the postoperative period in Groups 1 and 2 than in Group 3 (p = 0.003). Hemoglobin levels at 24 and 48 h postoperatively were significantly lower in Group 3 than in Group 1 (p < 0.001, each) and Group 2 (p = 0.038, p < 0.001). At 6, 24, and 48 h follow-ups, pain values were significantly lower in Group 2 than in Group 3 (p < 0.001). Preoperative 6, 24, and 48 h temperature values were significantly lower in Group 1 than in Group 3 (p < 0.001 for each). It was found that the difference between preoperative and postoperative knee flexion measurements was significantly different in both groups or the difference between the groups was changed in each period (p < 0.001). Conclusions: Postoperative cryotherapy is a potentially simple, noninvasive option and beneficial for the reduction of reducing pain, bleeding, length of stay, analgesic requirement and swelling after total knee arthroplasty. Moreover, there was no early or late prosthesis infection in cryotherapy groups, which may be considered as an additional measure to prevent prosthesis infection.

scholarly journals Correlation of invitro susceptibility based on MICs and SQLE mutations with clinical response to terbinafine in patients with tinea corporis/cruris

2018 ◽  
Author(s):  
Ananta Khurana ◽  
Aradhana Masih ◽  
Anuradha Chowdhary ◽  
Kabir Sardana ◽  
Sagar Borker ◽  
...  
Keyword(s):  
Clinical Response ◽  
Drug Exposure ◽  
Antifungal Susceptibility ◽  
Tinea Corporis ◽  
Squalene Epoxidase ◽  
Improved Outcomes ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

ABSTRACTRecalcitrant dermatophytoses are on the rise and recent publications have documented high minimum inhibitory concentrations (MICs) to TRB and squalene epoxidase (SQLE) mutations. However, literature correlating the laboratory the data with clinical response is lacking.This study was conducted to study the clinico-mycological profile of tinea corporis and cruris, including antifungal susceptibility testing (AFST) and SQLE mutation analysis and correlate these with clinical response to TRB. Skin scrapings of patients with tinea corporis with/without tinea cruris were subjected to species identification, AFST and SQLE gene analysis (on 15 isolates). KOH confirmed cases were started on TRB 250mg once a day (OD). If >50% clinical clearance was achieved by 3 weeks; the same dose was continued.(Group 1). If clinical clearance at 3 weeks was <50%, the dose was increased to 250mg twice a day (BD) (Group 2). If the response still remained below 50% after 3 weeks of BD, the patients were treated with itraconazole (ITR)(Group 3). Trichophyton interdigitale was confirmed on all 64 isolates obtained on culture. Forty four (68.7%) isolates had high (≥1 μg/ml) MICs to TRB. Six isolates were found to have aminoacid substitution Leu393Phe in SQLE protein, while one had the substitution Phe397Leu. The difference in modal MICs to TRB between the 3 clinical response groups (1.5157μg/ml, 5.0396 μg/ml and 20.1587μg/ml respectively for group 1,2 and 3) was highly significant. Clinical response was achieved in 68% of those resistant by MIC data, and 42.8% of SQLE mutation harboring isolates, by increasing drug (TRB) exposure.We infer that TRB resistance in dermatophytes has reached alarming proportions in our patients. Though improved outcomes were achieved with higher drug exposure, with the high failure rate seen in the study, the case for shifting to another class of antifungals as first line agent against dermatophytoses is strong.

scholarly journals Parasitemia of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi Pidie and Pemalang Isolate

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Febiola Rama Sari ◽  
Yudha Fahrimal ◽  
Ummu Balqis ◽  
Didik T Subekti ◽  
Aprilia Wardana
Keyword(s):  
Treatment Group ◽  
Trypanosoma Evansi ◽  
Rapid Rise ◽  
Treatment Groups ◽  
Parasitemia Level ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Short Time ◽  
Group 1

This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor. A total of 12 mice were divided into 3 treatment groups. Group 1 (K1) without any treatment, group 2 (K2) was inoculated with 104 T. evansi Pidie isolate, and group 3 (K3) was inoculated with 104 T. evansi Pemalang isolate. Parasitemia examination was carried out every two days and the level of parasitemia was observed as well. Parasitemia examination was conducted until all mice died. Parasitemia of mice infected with Pidie isolate was characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. The parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal was dead. In conclusion, there was the difference in parasitemia level between Pidie and Pemalang isolates. Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate

scholarly journals Analysis of Instagram® Posts Referring to Cleft Lip

2020 ◽  
Vol 17 (20) ◽  
pp. 7404
Author(s):  
Magdalena Sycinska-Dziarnowska ◽  
Piotr Stepien ◽  
Joanna Janiszewska-Olszowska ◽  
Katarzyna Grocholewicz ◽  
Maciej Jedlinski ◽  
...  
Keyword(s):  
Congenital Anomaly ◽  
Social Media ◽  
Cleft Lip ◽  
Medical Information ◽  
Cleft Lip And Palate ◽  
Group 4 ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

Background: Social media has become a source of medical information. Cleft lip and palate is a visible congenital anomaly. The aim of the study was to analyze Instagram® posts on the topic of cleft lip. Methods: Instagram® posts with “#cleftlip” from March 2014–March 2017 were accessed. Separate lists of expressions (hashtags, meaningful words, words with emojis or emojis alone) were prepared for primary posts and for replies. Thirty expressions statistically most frequent in primary versus secondary posts and 30 in secondary versus primary posts were identified (Group 1) as well as 30 English words or hashtags (Group 2), non-English words or hashtags (Group 3) and emojis (Group 4). The frequencies of expressions were compared (Z-test for the difference of two population proportions). Results: There were 34,129 posts, (5427 primary posts and 28,702 replies), containing 62,163 expressions, (35,004 in primary posts). The occurrence of all expressions was 454,162, (225,418 in primary posts and 228,744 in replies). Posts with positive expressions such as “beautiful”, “love”, “cute”, “great”, “awesome” occurred more often than these with negative ones. In replies all emojis were positive. Conclusions: Numerous Instagram® posts referring to cleft lip are published and do provoke discussion. People express their solidarity and sympathize with persons affected by cleft.

Machaut's balades with four voices

2001 ◽  
Vol 10 (1) ◽  
pp. 47-79 ◽  
Author(s):  
ELIZABETH EVA LEACH
Keyword(s):  
Fourteenth Century ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

Based on principles found in fourteenth-century counterpoint treatises, this study divides Machaut's balades with four voices into three groups according to their performance possibilities. The pieces of group 1 (Se quanque amours, Il mest avis, De toutes flours, and Quant theseus/Ne quier) can be performed either in all four parts or in three parts by omitting the triplum. Group 2 (De petit po and De Fortune plus Vg's redaction of Se quanque amours) includes those pieces that can be performed in three parts with either contratenor or triplum but not with all four parts. Pieces in this group are often found with all four parts copied together, thus highlighting the difference between the presentation of a piece in the sources and its performance options. The pieces in group 3 (En amer and Dame de qui) are less clearly delimited in their performance possibilities.

scholarly journals The effect of difference in saliva pH against Hg release from amalgam restoration on in vitro research

2007 ◽  
Vol 19 (2) ◽  
Author(s):  
Oksana Megasari
Keyword(s):  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Group 4 ◽  
Amalgam Restoration ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
The One ◽  
Group 1

Hg release from amalgam restoration is continuos as long as an amalgam in the mouth. The difference in saliva pH is one factor that influences Hg releasing from amalgam restoration. The purpose of this research was to find data the effect of the difference in saliva pH against Hg release from amalgam restoration. This research was a true experimental in vitro research. This research used 40 samples of premolar teeth of the maxilla, prepared in occlusal Class I, restored with amalgam and then divided into 4 Groups. Teeth belonging to Group 1 were immersed in saliva artificial with a pH content 5, Group 2 with a pH content of 6, Group 3 with a pH content of 7 as a control, and Group 4 with a pH content of 8. All tooth Groups were immersed for one week after condensation. Research data results analyzed using the One-Way Analysis of Variance (ANOVA). Research results measured using the Atomic Absorption Spectrophotometry (AAS) indicated that Group 1 with a pH content of 5 had the highest average release, namely, 19,276 ppb, followed by Group 4 with a pH content of 8, with a Hg release of 17,691 ppb, followed by Group 3 with a pH content of 7 as a control, with a Hg release of 13,702 ppb, and Group 2 with a pH content of 6 the lowest Hg release, namely 12,377 ppb. Summary of this research showed that there was no effect of saliva pH difference against Hg release from amalgam restoration.

scholarly journals 455. Diagnostic Value of Hematological Parameters in Different Stages of the Disease in Covid-19 Patients

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S330-S330
Author(s):  
Ruhma Ali ◽  
Aditya Patel ◽  
Kok Hoe Chan ◽  
Jihad Slim
Keyword(s):  
Disease Stage ◽  
Categorical Variables ◽  
P Value ◽  
Baseline Characteristic ◽  
Lymphocyte Ratio ◽  
Aggregate Index ◽  
Group 3 ◽  
The Difference ◽  
Group 2 ◽  
Group 1

Abstract Background COVID-19 infection is associated in some individuals with a rapid onset of systemic proinflammatory state leading to cytokine storm followed by multisystem organ failure. We are interested in studying the prognostic value of complete blood count parameters in different stages of the diseases based on the serology. Methods This is a retrospective cohort study of patients with confirmed COVID-19 admitted to our hospital between 10/1/2020 to 2/28/2021. Study individuals had complete CBC profile and COVID-19 serology with well-defined clinical outcome (discharged alive or expired). They were divided in 3 groups based on serology results: group 1 (early disease) had no antibodies, group 2 (immune phase) had + IgM, and group 3 (late phase) had only + IgG. Demographic, clinical and laboratory data were reviewed. Simple t-test was used for continuous variables and chi-square test was used for categorical variables. Anova test was used to compare the difference across multiple groups. GraphPad PRISM was used for all analysis. Results A total of 202 confirmed covid 19 cases were included in the study. There was no difference between the 3 groups in terms of age, gender, and body mass index (BMI). We did observe an increase in incidence in Latinx (group 1, 34%; group 2, 51%; group 3, 38%). Hypertension and diabetes were major co-morbidities in these patients. Absolute neutrophil count (ANC) and platelet count (PC) showed significant changes across the 3 groups: mean ANC for group 1, 4.868 (SD 3.117); group 2, mean 6.951 (SD 3.843); and group 3 mean 5.59 (SD 3.236). PC in group 1 mean 193.2 (SD 90.25); group 2 mean 271.1 (SD 143.4); and group 3 mean 228.6 (SD 75.33) p-value 0.0008. The difference can be seen in the derived monocyte platelet rationMPR, neutrophil lymphocyte ratio NLR, platelet lymphocyte ratio PLR and aggregate index of systemic inflammation AISI values and they tend to be higher in group 2 (MPR p-value 0.0067, NLR p-value 0.0123, PLR p-value 0.0294, AISI p-value of 0.0190). Baseline characteristic CBC parameters Conclusion The study demonstrates that MPR, NLR, PLR and AISI have a potential role in categorizing the disease stage based on only CBC profiling.Properly designed prospective studies with a larger sample size should be performed to confirm the disease stratification ability of derived CBC indices like MPR, NLR, PLR and AISI. Disclosures All Authors: No reported disclosures

The Urinary 5-Hydroxyindole Acetic Acid and Plasma Nitric Oxide Levels in Irritable Bowel Syndrome: A Preliminary Study

2005 ◽  
Vol 50 (1) ◽  
pp. 27-29 ◽  
Author(s):  
A Yazar ◽  
K Büyükafpar ◽  
G Polat ◽  
C Pata ◽  
A Kanýk ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Irritable Bowel Syndrome ◽  
Acetic Acid ◽  
Venous Blood ◽  
Group 3 ◽  
Preliminary Study ◽  
The Difference ◽  
Group 2 ◽  
Irritable Bowel ◽  
Group 1

Background and Aims: Postprandial increase of 3-hydroxytryptamine (5-HT) has been implicated in irritable bowel syndrome (IBS). There is evidence that nitric oxide (NO) may act as a mediator of 5-HT-evoked secretions in the colon. Our aim is to investigate the role of urinary 5-hydroxyindole acetic acid (5-HIAA) and plasma NO levels (with diarrhoea) in IBS patients. Methods: Nineteen (with constipation) IBS patients (group 1), 22 IBS patients (group 2) and 18 healthy controls (group 3) were included in the study. The diagnosis of IBS was made according to the Rome I Criteria. The urine was collected for determination of 5-HIAA and venous blood was collected from each subject for the measurement of plasma NO levels. Results: The levels of urinary 5-HIAA mmol/day and plasma NO mmol/l of group 1 (22,4±2,2 and 29,4±2 respectively) were significantly higher than group 3(14,2 ± 2,3 and 21,3 ± 2,1 respectively) (p =0,036 and p =0,019 respectively). The NO level of group 1 was also significantly higher than group 2(21,8 ± 1,9) (p = 0,021). The 5-HIAA level of group 1 was higher than group 2 (15,2, ± 2,1) and the difference was marginally significant (p = 0,055). There was no difference between group 2 and group 3 with respect to 5-HIAA and NO levels. Conclusions: The results of this preliminary study lend support to the involvement of 5-HT in some symptomatology of diarrhoea predominant IBS. Furthermore, NO may be one of the effector mediators of the 5-HT-induced symptoms in these patients.

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