Absorption, Metabolism, and Translocation of 2,4-D by Honeyvine Milkweed

Weed Science ◽  
1970 ◽  
Vol 18 (5) ◽  
pp. 653-656 ◽  
Author(s):  
H. D. Coble ◽  
F. W. Slife ◽  
H. S. Butler

Absorption of (2,4-dichlorophenoxy)acetic acid (2,4-D) by honeyvine milkweed [Ampelamus albidus(Nutt.) Britt.] was 7.2, 9.3, and 10.9% of the applied herbicide at 1, 4, and 8 days after treatment, respectively. Addition of 1.0% v/v Tween 80 to the treatments increased absorption to 55.8, 71.3, and 78.7% at the same sampling dates. Metabolic alteration of the herbicide occurred only in the aerial portions of the plant, and this was not great enough to be considered as a means of resistance. Translocation of 2,4-D into the roots was more closely related to new root growth than to the amount of top growth. Plants with six leaves and no new root growth did not translocate 2,4-D into the root zone. Plants with the same number of leaves but with an average of five and 20 new roots translocated 2.5 and 7.7%, respectively, of the applied herbicide to the roots.

Weed Science ◽  
1972 ◽  
Vol 20 (1) ◽  
pp. 31-33 ◽  
Author(s):  
D. N. Hyder ◽  
L. D. Sabatka

Mortality rates of Geyer larkspur(Delphinium geyeriGreene), a poisonous plant, were determined after spraying with (2,4-dichlorophenoxy)acetic acid at various stages of plant development in 1967 to 1969. Sprays of 2,4-D at 2.2 kg/ha were more effective at early than at late stages of development, but seldom exceeded 40% mortality. Uneven-aged stands and early drying (dormancy) of small non-flowering plants often prevented the exposure of all plants to a single application of spray; treated plants contorted but continued growth for 5 to 8 weeks after spraying; residual seed provided a source of new plants; and previously dormant rootstock buds gave rise to new shoot and root growth the year after spraying. These characteristics and escape mechanisms direct attention to possibilities of improving effectiveness by spraying two or three times in a single season.


HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 569-571 ◽  
Author(s):  
A. Raymond Miller ◽  
Craig K. Chandler

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).


Crop Science ◽  
1986 ◽  
Vol 26 (2) ◽  
pp. 376-377 ◽  
Author(s):  
Cecil Regier ◽  
R. E. Dilbeck ◽  
D. J. Undersander ◽  
J. E. Quisenberry

Weed Science ◽  
1971 ◽  
Vol 19 (6) ◽  
pp. 701-705 ◽  
Author(s):  
R. J. Burr ◽  
G. F. Warren

Several herbicides were tested in the greenhouse on ivyleaf morningglory (Ipomoea hederacea(L.) Jacq.), green foxtail (Setaria viridis(L.) Beauv.), purple nutsedge (Cyperus rotundusL.), and quackgrass (Agropyron repens(L.) Beauv.) to determine the degree of enhancement in activity that could be obtained with an isoparaffinic oil carrier applied at 140 L/ha. The enhancement varied with the herbicide and with the species, ranging from 16-fold enhancement with 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine) and 2-sec-butyl-4,6-dinitrophenol (dinoseb) on ivyleaf morningglory to no enhancement of atrazine activity on purple nutsedge and quackgrass or (2,4-dichlorophenoxy)acetic acid (2,4-D) activity on quackgrass and ivyleaf morningglory. An oil adjuvant was less effective in enhancing dinoseb and 3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea (linuron) activity than was the isoparaffinic oil carrier. Also, the isoparaffinic oil carrier emulsified in water was less effective than the undiluted oil in enhancing dinoseb activity on green foxtail, even though equal volumes of the isoparaffinic oil were applied.


2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.


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