A Rapid Microtitre Plate Assay for Determining Sensitivity to Photosystem II Herbicides

Weed Science ◽  
1994 ◽  
Vol 42 (4) ◽  
pp. 517-522 ◽  
Author(s):  
Michael P. Anderson ◽  
Curtis N. Bensch ◽  
Jimmy F. Stritzke
Keyword(s):  
Photosystem Ii ◽  
Thylakoid Membranes ◽  
Microtitre Plate ◽  
Plate Assay ◽  
Conventional Procedure ◽  
Microtitre Plate Assay ◽  
Reduction Assay ◽  
Before And After ◽  
Chlorophyll Concentrations

This paper reports on a microtitre plate version of the 2,6-dichlorophenol-indophenol (DCPIP) reduction assay. DCPIP reduction rates of alfalfa thylakoid membranes were determined by measuring absorbance at 600 nm before and after a 1 min illumination period. The membranes were near light-saturated at intensities above 600 μE m–2s–1. Saturation of thylakoid membranes with DCPIP occurred above 120 μM. DCPIP reduction rates increased linearly with chlorophyll concentrations from 0.25 to 2 μg. The rate of DCPIP reduction was linear throughout a 2 min illumination period (R2= 0.99). The assay was sensitive enough to terbacil to differentiate between a 20% change in the I50concentration. DCPIP reduction rates were sensitive to concentrations of terbacil as low as 100 nM. It only takes 13 minutes to load and read 96 samples using the microtitre plate assay compared to 5.5 h using the conventional procedure.

scholarly journals Microtitre Plate Assay for the Quantification of Biofilm Formation by Pathogenic Leptospira

2017 ◽  
Vol 12 (2) ◽  
pp. 146-153 ◽  
Author(s):  
Kasing Apun ◽  
Chai Fung Pui ◽  
Jennifer Jalan ◽  
Lesley Maurice Bi ◽  
Lela Su`ut ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Microtitre Plate ◽  
Pathogenic Leptospira ◽  
Plate Assay ◽  
Microtitre Plate Assay

A microtitre plate assay for measuring glycosidase activity

2008 ◽  
Vol 23 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Andrea L. Ball ◽  
Kirsty A. Chambers ◽  
Meera Hewinson ◽  
Sambavi Navaratnarajah ◽  
Lamia Samrin ◽  
...  
Keyword(s):  
Microtitre Plate ◽  
Plate Assay ◽  
Glycosidase Activity ◽  
Microtitre Plate Assay

A microtitre plate assay for the detection of antibiotics in porcine urine

The Analyst ◽  
2000 ◽  
Vol 125 (3) ◽  
pp. 395-396 ◽  
Author(s):  
Robert K. Buick ◽  
Nuala M. Greer ◽  
Christopher T. Elliott
Keyword(s):  
Microtitre Plate ◽  
Plate Assay ◽  
Microtitre Plate Assay

scholarly journals Antibiogram and Biofilm Formation among Carbapenem Resistant Klebsiella pneumoniae

2019 ◽  
Vol 6 ◽  
pp. 63-69
Author(s):  
Roshani Nhuchhen Pradhan ◽  
Surendra Kumar Madhup ◽  
Shyam Prasad Pant
Keyword(s):  
Klebsiella Pneumoniae ◽  
Congo Red ◽  
Biofilm Formation ◽  
Microtitre Plate ◽  
Sensitivity Testing ◽  
Clinical Specimens ◽  
Plate Assay ◽  
Carbapenem Resistant ◽  
Microtitre Plate Assay ◽  
Agar Method

Objectives: This cross-sectional study was designed to detect the carbapenemase producing K. pneumoniae along with biofilm producers from different clinical specimens and to compare antibiotic susceptibility pattern of biofilm producing carbapenem resistant Klebsiella pneumoniae and biofilm non-producing carbapenem resistant Klebsiella pneumoniae. Methods: A total of 1475 non-repetitive clinical samples were included on this study. Antibiotic Sensitivity Testing (AST), Modified Hodge Test (MHT) and Modified Carbapenem inactivation method (mCIM) were performed for detection of carbapenemase production and Congo red agar method (CRA) along with Microtitre plate method were performed for detecting biofilm production. Results: Among the clinical specimens cultured, growth positivity was 62.71%. E. coli was most predominant organism followed by K. pneumoniae (17.89%). Among the 110 K. pneumoniae, 57 were found to be carbapenemase producer. Majority of the carbapenemase producing K. pneumoniae were isolated from sputum (45.61%), in the specimen collected from age group 61-70 (28.07%) and in out-patient department (50.88%). Similarly, 65.45% K. pneumoniae out of 110 were found to be biofilm producer by Congo red agar method while among those 72, 73.59% isolates were found to be quantitatively biofilm producer in Microtitre plate assay. Out of 57 carbapenemase producer, 35.08% were strongly biofilm producer while among 53 carbapenemase non-producer 30.18% were strongly biofilm producer from Congo red agar method. Moreover, Microtitre plate assay evidenced that, out of 57 carbapenemase producer, 40.35% were highly biofilm producing and among the 15 carbapenemase nonproducer 66.66% were highly biofilm producer.  Conclusion: Biofilm formation is highly prevalent with varying degree of resistance among different antibiotics including carbapenems that further augments antibiotic resistance. The study showed carbapenemase producers are stronger biofilm producer than the non-carbapenemase producer. Therefore, it is recommended to identify biofilm formation among carbapenemase producers for effective choice of antibiotics.

scholarly journals A microtitre plate assay for characterizing insensitive acetylcholinesterase genotypes of insecticide-resistant insects

1988 ◽  
Vol 78 (3) ◽  
pp. 537-544 ◽  
Author(s):  
Graham D. Moores ◽  
Alan L. Devonshire ◽  
Ian Denholm
Keyword(s):  
Musca Domestica ◽  
Microtitre Plate ◽  
Time Data ◽  
Rapid Technique ◽  
Plate Assay ◽  
Carbamate Insecticides ◽  
Microtitre Plate Assay ◽  
Microplate Reader ◽  
Presence And Absence ◽  
Linear Regressions

AbstractA rapid technique is described for characterizing and monitoring, in single insects, the insensitivity of acetylcholinesterase (AChE) to organophosphorus and carbamate insecticides. Ninety-six insects are homogenized simultaneously in a microtitre plate and portions (e.g. 0·05 for Musca domestica L.) assayed colorimetrically with acetylthiocholine in the presence and absence of diagnostic concentrations of insecticide. Reactions are monitored by a kinetic microplate reader linked to a microcomputer that determines mean AChE activities automatically by linear regressions of absorbance-time data. Mean inhibited activity is then expressed as a percentage of uninhibited activity. Several inhibitors can be tested against the same insect to yield an ‘insensitivity profile‘ of individuals and strains. In tests on M. domestica adults of known AChE genotype, the assay clearly distinguished not only between a sensitive and two slightly (3-15-fold) insensitive AChE variants but between all six genotypic combinations of these three alleles.

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