Molecular markers indicate intraspecific variation in the control ofEchinochloaspp. with quinclorac

Weed Science ◽  
1999 ◽  
Vol 47 (3) ◽  
pp. 310-315 ◽  
Author(s):  
Nuria Lopez-Martinez ◽  
Antonio Pujadas Salva ◽  
Robert P. Finch ◽  
George Marshall ◽  
Rafael De Prado
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Intraspecific Variation ◽  
Morphological Diversity ◽  
Differential Sensitivity ◽  
Yield Losses ◽  
Natural Tolerance ◽  
Botanical Classification

Some species of the genusEchinochloaare troublesome weeds in rice fields. The taxonomy of this genus leads to confusion in many cases due to its great morphological diversity. Because of the differential sensitivity to the herbicide quinclorac shown byEchinochloaspp., it was necessary to assess the botanical and molecular characterization of this weed.Echinochloa colonum, E. oryzoides, andE. oryzicolawere very susceptible to quinclorac treatment; by contrast,E. crus-galliandE. hispidulashowed some degree of natural tolerance. Physiological and molecular results agreed with the botanical classification of the genusEchinochloain Flora Europea. The importance of these results is due to yield losses produced by the infestation ofEchinochloaand the need for a strategy forEchinochloamanagement depending on the distribution of theEchinochloaspecies.

scholarly journals Molecular characterization and distribution of Plasmodium matutinum, a common avian malaria parasite

Parasitology ◽  
2017 ◽  
Vol 144 (13) ◽  
pp. 1726-1735 ◽  
Author(s):  
GEDIMINAS VALKIŪNAS ◽  
MIKAS ILGŪNAS ◽  
DOVILĖ BUKAUSKAITĖ ◽  
VAIDAS PALINAUSKAS ◽  
RASA BERNOTIENĖ ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Malaria Parasite ◽  
Avian Malaria ◽  
Clinical Signs ◽  
Molecular Data ◽  
Infected Blood ◽  
Avian Malaria Parasite ◽  
Thrush Nightingale

SUMMARYSpecies of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained. Domestic canaries Serinus canaria were susceptible after inoculation of infected blood, and the long-lasting light parasitemia developed in two exposed birds. Clinical signs of illness were not reported. Illustrations of blood stages of P. matutinum (pLINN1) are given, and phylogenetic analysis identified the closely related avian Plasmodium species. The phylogeny based on partial cytochrome b (cyt b) sequences suggests that this parasite is most closely related to Plasmodium tejerai (cyt b lineage pSPMAG01), a common malaria parasite of American birds. Both these parasites belong to subgenus Haemamoeba, and their blood stages are similar morphologically, particularly due to marked vacuolization of the cytoplasm in growing erythrocytic meronts. Molecular data show that transmission of P. matutinum (pLINN1) occurs broadly in the Holarctic, and the parasite likely is of cosmopolitan distribution. Passeriform birds and Culex mosquitoes are common hosts. This study provides first molecular markers for detection of P. matutinum.

scholarly journals Morphologic and molecular characterization of Myrciaria spp species

2005 ◽  
Vol 27 (3) ◽  
pp. 507-510 ◽  
Author(s):  
Márcio Pereira ◽  
Antonio Luis de Oliveira ◽  
Regina Eli de Almeida Pereira ◽  
Janete Apparecida Desidério Sena ◽  
Juliana Regina Vieira da Costa ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Rapd Markers ◽  
Fruit Trees ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Morphologic Characteristics ◽  
Botanical Classification ◽  
Made In

The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and molecular differences between the studied plants were identified and four groups of species were defined: Myrciaria cauliflora (Mart.) O. Berg, M. coronata Mattos, M. jaboticaba (Vell.) O. Berg. and Myrciaria phytrantha (Kiaersk.) Mattos. Both molecular and morphologic markers techniques showed to be important tools for the identification of jabuticaba tree species.

Molecular characterization of Greek pepper (Capsicum annuum L) landraces with neutral (ISSR) and gene-based (SCoT and EST-SSR) molecular markers

2015 ◽  
Vol 59 ◽  
pp. 256-263 ◽  
Author(s):  
Aphrodite Tsaballa ◽  
Ioannis Ganopoulos ◽  
Antonia Timplalexi ◽  
Xanthopoulou Aliki ◽  
Irene Bosmali ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Capsicum Annuum ◽  
Capsicum Annuum L

Molecular characterization of intra-population variability of Jatropha curcas L. using DNA based molecular markers

2011 ◽  
Vol 39 (4) ◽  
pp. 4383-4390 ◽  
Author(s):  
Shaik G. Mastan ◽  
Pamidimarri D. V. N. Sudheer ◽  
H. Rahman ◽  
A. Ghosh ◽  
Mangal S. Rathore ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Jatropha Curcas ◽  
Population Variability ◽  
Jatropha Curcas L

Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping

2006 ◽  
Vol 8 (3) ◽  
pp. 860-865 ◽  
Author(s):  
Cinzia Marianelli ◽  
Franco Ciuchini ◽  
Michela Tarantino ◽  
Paolo Pasquali ◽  
Rosanna Adone
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Rpob Gene ◽  
Brucella Species

scholarly journals Characterization of rbcL and trnL Plastid DNA Sequences of Vuralia turcica (Fabaceae; Papilionoideae)

2019 ◽  
Vol 7 (6) ◽  
pp. 908
Author(s):  
Dilek Tekdal
Keyword(s):  
Molecular Markers ◽  
Phylogenetic Tree ◽  
Plant Species ◽  
Dna Sequences ◽  
Its Region ◽  
Plastid Dna ◽  
Accession Number ◽  
Molecular Information

Vuralia turcica is endemic to Turkey and currently endangered. Little molecular information is available for this plant. Previous characterization and classification of V. turcica have been based on the DNA sequences of the ITS region. Molecular markers are essential for studying of genotyping and biogeography, but any of each marker is not enough to characterize a plant species in its use alone. In this study, the chloroplast rbcL and trnL regions were amplified in V. turcica using the primers that have been published in the previous studies. Successfully amplified DNA fragments were extracted and commercially sequenced. The partial rbcL and trnL sequences were submitted to the NCBI database (accession number KX164510, KX164511, respectively). Amplified both DNA of two regions of rbcL and trnL were used to construct a phylogenetic tree.

scholarly journals Caracterización morfo-agronómica y molecular de mandarina ‘Arrayana‘ en el piedemonte del Meta (Colombia)

2012 ◽  
Vol 13 (1) ◽  
pp. 5 ◽  
Author(s):  
Javier Orlando Orduz-Rodríguez ◽  
Javier Monroy ◽  
Sandra Barrera ◽  
Víctor Núñez ◽  
Gustavo Ligarreto
Keyword(s):  
Molecular Markers ◽  
Genetic Resources ◽  
Molecular Characterization ◽  
Clonal Propagation ◽  
Agronomic Traits ◽  
Research Center ◽  
Morphological Description ◽  
Citrus Reticulata ◽  
Molecular Variation

<p>Debido a la ausencia de información varietal de la mandarina (<em>Citrus reticulata </em>L.) ‘Arrayana‘ en el piedemonte Llanero del departamento del Meta (Colombia), se realizó una caracterización morfo-agronómica y molecular de árboles comerciales de mandarina de 10 años de edad del Centro de Investigación La Libertad de Corpoica ubicado en Villavicencio (Meta). Para la descripción morfológica se utilizaron los descriptores sugeridos por el Instituto Internacional de Recursos Genéticos (IPGRI), mientras que en la caracterización molecular, se utilizó la técnica de marcadores moleculares del tipo AFLP. La variación molecular detectada por AFLP mostró en los diez individuos de la mandarina ‘Arrayana‘ los mismos fragmentos para cada una de las accesiones, identificando individuos genéticamente iguales formando un solo grupo, lo que estaría relacionado con la propagación clonal y con características nucelares. Igualmente, se detectó uniformidad en los caracteres morfo-agronómicos, relacionados con las principales características hortícolas, lo cual señala niveles de homogeneidad, que permiten clasificar la población como una variedad.</p><p> </p><p> <strong>Morpho-agronomic and molecular characterization of the ‘Arrayana‘ mandarin in the piedmont of the Meta department (Colombia) </strong></p><p>Due to the absence of varietal information for the tangerine (<em>Citrus reticulata </em>L.) ‘Arrayana‘ in the piedmont plains of Meta (Colombia), morpho-agronomic and molecular characterization of 10-year-old commercial tangerine trees was carried out at the Research Center La Libertad of Corpoica in Villavicencio (Meta). Descriptors suggested by the International Genetic Resources Institute (IPGRI) were used for the morphological description, while for molecular characterization, we used the AFLP type molecular markers technique. The molecular variation detected by AFLP showed the same fragments for each of the accessions in ten individuals of the tangerine ‘Arrayana‘, identifying genetically identical individuals, forming a group; which might be related to clonal propagation and nucellar features. Similarly, we detected uniform morpho-agronomic traits related to major horticultural characteristics, which indicates levels of homogeneity for classifying the population as a variety.</p>

Molecular characterization of European Pygorchis Looss, 1899

2021 ◽  
Vol 95 ◽  
Author(s):  
P. Heneberg ◽  
J. Sitko
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences ◽  
Type Species ◽  
Molecular Phylogenetics ◽  
Morphological Description ◽  
Central Asian ◽  
First Time

Abstract Adult trematodes of the genus Pygorchis Looss, 1899 (Trematoda: Philophthalmidae) parasitize the cloaca of birds. The genus contains three species, all of which are rarely reported and molecular phylogenetics of which have not been applied. The absence of reference DNA sequences limit studies of their indistinct larval forms. Based on the materials that were obtained from birds of the Czech origin, we performed a molecular characterization of both currently known Pygorchis spp., which are known from the Palearctic, the type species Pygorchis affixus Looss, 1899 and Pygorchis alakolensis Zhatkanbaeva, 1967, and provided morphological description of the examined P. alakolensis specimen. We found that the two species were of similar dimensions; the only difference was in the position of testes and in the extent of vitelline follicles. However, the position of testes in P. affixus was variable, and approximately 10% of examined P. affixus individuals had testes positioned obliquely. The second feature that allows differential diagnostic, the extent of vitelline follicles, was more reproducible as the vitelline follicles of P. affixus did not extend beyond the intestinal caeca, or, in exceptional cases, they extended them at only one side. In the examined P. alakolensis individual, the testes were positioned obliquely, and the vitelline follicles extended beyond the intestinal caeca. We reported P. alakolensis for the first time from Europe; previously, it was known only from Central Asian lakes and rivers. We confirmed the classification of Pygorchis into Philophtalmidae.

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