Viability of Staphylococci in Various Diluents

1986 ◽  
Vol 7 (7) ◽  
pp. 370-372 ◽  
Author(s):  
Razmick Essiain ◽  
D.J Flournoy
Keyword(s):  
Peritoneal Dialysis ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Tap Water ◽  
Skim Milk ◽  
Deionized Water ◽  
Dialysis Fluid ◽  
Peritoneal Dialysis Fluid ◽  
Better Than

AbstractStandardized suspensions of 78 staphylococci and micrococci were stored in different diluents and tested periodically for 3 weeks to determine their ability to maintain viability. In 92% of the tests, diluent suspensions yielded viable organisms for at least 21 days. Organism survival was maintained in the following order, from best to worst: skim milk, tap water, 0.9% NaCl, deionized water, Dianeal (peritoneal dialysis) fluid, 0.2% bovine serum albumin and 5% glycerol. In 81% of the instances, oxacillin-susceptible staphylococci survived better than oxacillin-resistant staphylococci.

Adsorption of bovine serum albumin on nano and bulk oxide particles in deionized water

2012 ◽  
Vol 94 ◽  
pp. 341-346 ◽  
Author(s):  
Lei Song ◽  
Kun Yang ◽  
Wei Jiang ◽  
Peng Du ◽  
Baoshan Xing
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Deionized Water ◽  
Oxide Particles

The Friction and Wear of Silicon Nitride Ceramic with BSA Lubricant

2015 ◽  
Vol 642 ◽  
pp. 125-129
Author(s):  
Jing Ling Zhou ◽  
Chun Shu Zhai ◽  
Su Yun Yang ◽  
Shu Qian Wu ◽  
Guo Qing Wu ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Silicon Nitride ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Friction And Wear ◽  
Physiological Saline ◽  
Deionized Water ◽  
Silicon Nitride Ceramic ◽  
Ceramic Ball

To research the friction and wear of silicon nitride ceramic with bovine serum albumin lubricant, the tribological properties of silicon nitride ceramic against stainless steel were investigated on CETR UMT-2 under lubrication of bovine serum albumin, deionized water, physiological saline and physiological saline mixed with bovine serum albumin. The worn surfaces of silicon nitride ceramic ball and stainless steel pin were examined with a digital microscope (VHX-2000). The friction coefficients of steady state are 0.26, 0.35, 0.69 and 0.8 under bovine serum albumin, physiological saline mixed with bovine serum albumin, physiological saline and deionized water. The lowest friction coefficient of steady state is 0.26 which is under lubrication of bovine serum albumin. The highest friction coefficient is 0.8 under the lubrication of deionized water. The measured worn areas of silicon nitride ceramic balls are 1282.3μm2, 1898.6μm2, 2753.9μm2 and 3645.7μm2 under bovine serum albumin, physiological saline mixed with bovine serum albumin, physiological saline and deionized water. The smallest worn area of silicon nitride ceramic ball is 1282.3μm2 which is measured under the lubrication of bovine serum albumin. The highest worn area of silicon nitride ceramic ball is 3645.7μm2 which was measured under the lubrication of deionized water. The same wear mechanism of silicon nitride ceramic ball had been found under the lubrication of bovine serum albumin, deionized water, physiological saline and physiological saline mixed with bovine serum albumin. The depth of scratches of worn surface of silicon nitride ceramic ball lubricated with BSA is 3μm which are the shallowest.

263. Substitution of skim milk with bovine serum albumin in a stallion semen diluent

2008 ◽  
Vol 20 (9) ◽  
pp. 63
Author(s):  
Z. Gibb ◽  
C. G. Grupen ◽  
L. H. A. Morris ◽  
G. Evans ◽  
W. M. C. Maxwell
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Skim Milk ◽  
Lower Percentage ◽  
Animal Science ◽  
Flow Cytometric ◽  
Alternative Protein ◽  
Protective Proteins

Skim milk has long been utilised as a source of protective proteins in stallion semen diluents. However, skim milk is also thought to contain components that are toxic to sperm and reduces the clarity of sperm suspensions, which impedes sperm assessments. This may also reduce the effectiveness of staining procedures used to process sperm for flow cytometric sex-sorting. The aim of this study was to ascertain the optimal concentration of bovine serum albumin (BSA) to replace skim milk in a traditional stallion semen diluent, Kenney's Modified Tyrode's (KMT) Medium1, for handling and processing stallion sperm before flow cytometric sex-sorting. Two ejaculates were collected from each of three pony stallions. Each ejaculate was divided into five aliquots and diluted in either KMT with skim milk or KMT supplemented with 0, 0.25, 0.5 or 1% BSA. Diluted samples were further divided into two aliquots and either stored at 15°C for 18 h before incubation and assessment, or incubated and assessed immediately upon arrival. Samples were incubated at 34°C and evaluated at 0, 45 and 90 min for objective motility and acrosome integrity. No interactions were observed between any treatments over time. There was a lower percentage of intact and a higher percentage of detached acrosomes for sperm incubated in KMT containing 0% BSA than all other treatments. A greater proportion of sperm incubated in KMT with skim milk had partial acrosome damage compared with other treatments. There was no difference in % total motility for sperm incubated in KMT with skim milk, and KMT containing 0.5 and 1% BSA (Table 1). These results indicate that BSA may be suitable as an alternative protein source in stallion semen diluents. Further studies are required to compare sex-sorting rates and sperm quality after sex-sorting, incubation and staining in skim milk compared with BSA-based media. (1) Padilla, A W and Foote, R H 1991, ‘Extender and centrifugation effects on the motility patterns of slow-cooled stallion spermatozoa’, Journal of Animal Science, vol. 69, no. 8, pp. 3308–331

scholarly journals Surimi of king weakfish (Macrodon ancylodon) wastes: texture gel evaluation with protease inhibitors and transglutaminase

2004 ◽  
Vol 47 (6) ◽  
pp. 895-901 ◽  
Author(s):  
Cláudio Rafael Kuhn ◽  
Carlos Prentice-Hernández ◽  
João Luís Vendruscolo ◽  
Germano Jorge Dorneles Soares
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protease Inhibitors ◽  
Bovine Serum ◽  
Egg White ◽  
Gel Strength ◽  
Compression Force ◽  
Gel Texture ◽  
Better Than

The protease inhibitors (bovine serum albumin -BSA - and egg white) and transglutaminase inhibitor (NH4Cl), was added to the surimi obtained by King weakfish (Macrodon ancylodon) wastes to evaluate your effect on the gel texture. Results indicated that the treatment with pre-heating (60°C, 30min + 90°C, 15min) favored the elasticity of the gel (ashi), demonstrating low proteolysis and characterizing the suwari phenomenon (high gel strength). Protease inhibitors increased gel strength significantly (P<0.05) by compression force, when BSA was better than egg whites. Gels from surimi of King weakfish wastes didn't have action of the transglutaminase characterized.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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