Effect of non-digestible oligosaccharides in diets for weaner pigs on in vitro fermentation

1998 ◽  
Vol 1998 ◽  
pp. 30-30 ◽  
Author(s):  
J.G.M. Houdijk ◽  
B.A. Williams ◽  
S. Tamminga ◽  
M.W.A. Verstegen
Keyword(s):  
Gas Production ◽  
Production Technique ◽  
In Vitro Fermentation ◽  
Weaner Pigs

Dietary fructooligosaccharides (FOS) shifted the proportion of propionate (↑) and acetate (↓) compared to transgalactooligosaccharides (TOS) in weaner pigs' ileal digesta, both in vivo and in vitro (Houdijk et al., 1997). This could be related to different fermentation rates between these so-called non-digestible oligosaccharides (NDOs). These rates were studied via the cumulative gas production technique comparing FOS, TOS, and glucose.

Effect of non-digestible oligosaccharides in diets for weaner pigs on in vitro fermentation

1998 ◽  
Vol 1998 ◽  
pp. 30-30
Author(s):  
J.G.M. Houdijk ◽  
B.A. Williams ◽  
S. Tamminga ◽  
M.W.A. Verstegen
Keyword(s):  
Gas Production ◽  
Production Technique ◽  
In Vitro Fermentation ◽  
Weaner Pigs

Dietary fructooligosaccharides (FOS) shifted the proportion of propionate (↑) and acetate (↓) compared to transgalactooligosaccharides (TOS) in weaner pigs' ileal digesta, both in vivo and in vitro (Houdijk et al., 1997). This could be related to different fermentation rates between these so-called non-digestible oligosaccharides (NDOs). These rates were studied via the cumulative gas production technique comparing FOS, TOS, and glucose.

Relation between in vivo and in vitro fermentation of oligosaccharides in weaner pigs

1997 ◽  
Vol 1997 ◽  
pp. 57-59
Author(s):  
J.G.M. Houdijk ◽  
B.A. Williams ◽  
S. Tamminga ◽  
M.W.A. Verstegen
Keyword(s):  
Control Diet ◽  
Gas Production ◽  
Crude Fibre ◽  
In Vitro Fermentation ◽  
Sterile Saline ◽  
Weaner Pigs ◽  
Endogenous Enzymes ◽  
Cheese Cloth

Non-digestible oligosaccharides (NDOs), including fructo-oligosaccharides (FOS) and trans-galacto-oligosaccharides (TOS), are not digested by endogenous enzymes, but may be fermented by bacteria such as lactobacilli and bifidobacteria (Tomomatsu, 1994). Thus, dietary NDOs may alter fermentation characteristics of the microflora of the host. In this experiment, in vivo and in vitro fermentation characteristics of NDOs in weaner pigs were studied, using the cumulative gas production technique (Theodorou et al.,1994).A NDO-free control diet (CON), with oatshuskmeal (OHM) as a fibre source, and this diet with 40 g FOS (FOS40) or TOS (TOS40) per kg (w/w against cellulose) was restrictedly fed for 7 weeks (2.6*Em) to 3 groups of four 35-day old individually housed weaner pigs. Total faeces were collected from day 13-18 to determine crude fibre digestibility. Pigs were fitted with a PVTC-cannula on day 21 (Van Leeuwen et al, 1991). Inocula for gas production were anaerobically prepared from faeces (day 14-16) and ileal chyme (day 33-35, via PVTC-cannula). Samples were diluted (1:4) with sterile saline and filtered through cheese cloth. Five ml filtrate were injected into airtight bottles, containing 80 ml growth medium and no substrate (BLA) or 0.25 g FOS, TOS or OHM as sole energy source.

Stoichiometry of in vitro fermentation of pure substrates with particular emphasis on methane production, using the cumulative gas production technique

1996 ◽  
Vol 1996 ◽  
pp. 24-24
Author(s):  
Angela R. Moss ◽  
Karen C. O'Callaghan
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Fermentation

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal. In vitro techniques using rumen fluid as an innoculum can be considered as models of in vivo rumen digestion and have been applied to estimate digestibility of feedstuffs. The in vitro gas production technique (Menke et. al., 1979) can be used successfully to estimate this but there has been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.Three pure substrates, glucose (G), pectin (P) and cellulose (C) (lg) were pre-wetted in 94ml of medium D (Theodorou et. al., 1994). inoculated with strained rumen fluid (from wether sheep. 10ml) and incubated without agitation for 72h at 39°C.

Comparison of parameters from the Theodorou gas production technique using nitrogen-free and nitrogen-rich media as predictors of dry-matter intake and digestibility

1998 ◽  
Vol 22 ◽  
pp. 172-174
Author(s):  
D. L. Romney ◽  
F. C. Cadario ◽  
E. Owen ◽  
A .H. Murray
Keyword(s):  
Dry Matter ◽  
Gas Production ◽  
Dry Matter Intake ◽  
Production Technique ◽  
N Supply ◽  
Fermentation Method ◽  
Rich Media ◽  
Production Techniques

Parameters from in vitro gas production techniques could have potential as predictors of dry-matter intake (DMI) and digestibility. Fermentation is usually carried out under conditions where nitrogen (N) is not limiting. Therefore where N supply is a constraint to intake and digestibility, prediction equations may be inaccurate. This study compared the use of N-free and N-rich media in an in vitro fermentation method (Theodorou et al., 1994) and studied the relationships between in vitro and in vivo parameters obtained using both media.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

A comparison between in vitro digestibility, in situ degradability and a gas production technique for predicting the in vivo digestibility of whole-crop wheat

1998 ◽  
Vol 22 ◽  
pp. 33-35
Author(s):  
A. T. Adesogan ◽  
E. Owen ◽  
D. I. Givens
Keyword(s):  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Production Technique ◽  
Grass Silage ◽  
Laboratory Techniques

Several published reports on the nutritive value of whole-crop wheat (WCW) have been based on estimations from laboratory techniques, some of which were developed for grass silage. However, there is little information on the accuracy of such estimations. Therefore the aim of this study was to evaluate the suitability of predicting the in vivo digestibility of WCW from various less animal-dependent techniques.

Stoichiometry ofin vitrofermentation of pure substrates with particular emphasis on methane production, using the cumulative gas production technique

1996 ◽  
Vol 1996 ◽  
pp. 24-24
Author(s):  
Angela R. Moss ◽  
Karen C. O'Callaghan
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal.In vitrotechniques using rumen fluid as an innoculum can be considered as models ofin vivorumen digestion and have been applied to estimate digestibility of feedstuffs. Thein vitrogas production technique (Menkeet. al., 1979) can be used successfully to estimate this but there has been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.Three pure substrates, glucose (G), pectin (P) and cellulose (C) (lg) were pre-wetted in 94ml of medium D (Theodorouet. al., 1994). inoculated with strained rumen fluid (from wether sheep. 10ml) and incubated without agitation for 72h at 39°C.

Methane production from a range of feedstuffs as determined in vitro using the cumulative gas production technique and compared with that measured in vivo

1997 ◽  
Vol 1997 ◽  
pp. 194-194
Author(s):  
Angela R. Moss ◽  
D. I. Givens
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal. In vitro techniques using rumen fluid as an innoculum can be considered as models of in vivo rumen digestion and have been applied to estimate digestibility of feedstuffs. The in vitro gas production technique (Menke et. al, 1979) can be used successfully to estimate this but there have been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68 ◽  
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

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