An ultrastructural study of the pathogenesis of the arteriosclerotic lesions in the spontaneous hypertensive (SHR) rat strain

1978 ◽  
Vol 36 (2) ◽  
pp. 482-483
Author(s):  
G. Yajima ◽  
S. Kurihara ◽  
M. Ida ◽  
K. Aihara ◽  
G. Asano ◽  
...  
Keyword(s):  
High Frequency ◽  
Spontaneous Hypertension ◽  
Hypertensive Rats ◽  
Electron Microscopic ◽  
Morphological Aspects ◽  
Solid Diet ◽  
Specific Strain ◽  
Microscopic Studies ◽  
Rat Strain ◽  
The University

The spontaneous hypertensive rats are the strain which was isolated by Okamoto et al and is known to cause high frequency of spontaneous hypertension in later stage of life. Some of them are also recongnized to have high frequency of the cerebro-vascular accident and is also designated as apoplexy prone strain. The genetic and etiological factors of this spontaneous hypertension are the matters of the extensive survey of the investigators, however, the morphological aspects of this specific strain of causing the hypertension still await further confirmation in future. In this viewpoint, the authors have undertaken the electron microscopic and cytochemical electron microscopic studies of the various arteries in a rather extensive manner.The spontaneous hypertensive rats were supplied from the animal room of the University of Kyoto by courtesy of Drs. Okamoto and Yamori. The rats were kept on the commericially available solid diet and sytolic blood pressure was measured and scored daily.

scholarly journals Beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats

2012 ◽  
Vol 109 (10) ◽  
pp. 1806-1812 ◽  
Author(s):  
Nallasamy Palanisamy ◽  
Anuradha C. Venkataraman
Keyword(s):  
Blood Pressure ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Hypertensive Rats ◽  
Enos Expression ◽  
Electron Microscopic ◽  
Plasma Angiotensin ◽  
Enhanced Expression ◽  
Male Wistar Rats ◽  
Microscopic Studies

The study evaluates the effects of genistein on blood pressure (BP) and ultrastructural changes in kidney of fructose-fed hypertensive rats. Male Wistar rats were fed a diet containing 60 % starch or 60 % fructose as the source of carbohydrate. After 15 d, rats in each dietary group were divided into two groups and were treated with either genistein (1 mg/kg per d) in dimethylsulfoxide (DMSO) or 30 % DMSO alone. BP, pressor mechanisms, protein kinase C-βII (PKC-βII) expression, endothelial NO synthase (eNOS) expression and renal ultrastructural changes were evaluated after 60 d. Fructose-fed rats displayed significant elevation in BP and heart rate. Significant increase in plasma angiotensin-converting enzyme (ACE) activity, alterations in renal lipid profile, nitrite and kallikrein activity, enhanced expression of membrane-associated PKC-βII and decreased expression of eNOS were observed in them. Histology and electron microscopic studies showed structural changes in the kidney. Genistein administration lowered BP, restored ACE, PKC-βII and eNOS expression and preserved renal ultrastructural integrity. These findings demonstrate that genistein has effects on eNOS activity in renal cells, leading to eNOS activation and NO synthesis. These effects could have been mediated by activation of PKC-βII. The observed benefits of genistein make it a promising candidate for therapy of diabetic kidney disease.

Electron Microscopic Studies on the Morphogenesis of Fibrinoid Degeneration in the Mesenteric Arteries of Hypertensive Rats

Angiology ◽  
1965 ◽  
Vol 16 (1) ◽  
pp. 8-17 ◽  
Author(s):  
Genju Ooneda ◽  
Yasuo Ooyama ◽  
Kenji Matsuyama ◽  
Masamitsu Takatama ◽  
Yoji Yoshida ◽  
...  
Keyword(s):  
Mesenteric Arteries ◽  
Hypertensive Rats ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Fibrinoid Degeneration

The Thymus of Spontaneously Hypertensive Rats: Light- and Electron-Microscopic Studies

1973 ◽  
Vol 45 (s1) ◽  
pp. 141s-144s
Author(s):  
J. M. Rojo-Ortega ◽  
E. Yeghiayan ◽  
J. Genest
Keyword(s):  
Epithelial Cells ◽  
Vascular Disease ◽  
Spontaneously Hypertensive Rats ◽  
Plasma Cells ◽  
Vascular Lesions ◽  
Hypertensive Rats ◽  
Electron Microscopic ◽  
Spontaneously Hypertensive ◽  
Microscopic Studies ◽  
Microscopic Techniques

1. The present investigation was undertaken to study the possibility that hypertensive vascular disease could be associated with changes in the morphology of the thymus. 2. Using light- and electron-microscopic techniques, the thymus of spontaneously hypertensive rats (SHR) with (1 year of age) and without (10 weeks of age) severe vascular lesions were compared with appropriate controls. 3. The thymus of SHR with severe hypertensive vascular disease showed: (a) marked hyperplasia and hypertrophy of the epithelial cells, with ultrastructural features of hypersecretory activity, and (b) in contrast to the paucity of plasma cells in the thymus of the control animals, the SHR had numerous plasma cells. 4. These observations may suggest the involvement of an autoimmunological mechanism in the development of the vascular lesions in SHR.

Morphological aspects of the shedding of surface layers from peanut roots

1997 ◽  
Vol 75 (4) ◽  
pp. 607-611 ◽  
Author(s):  
Eiji Uheda ◽  
Yoko Akasaka ◽  
Hiroyuki Daimon
Keyword(s):  
Arachis Hypogaea ◽  
Cell Separation ◽  
Cell Walls ◽  
Separation Process ◽  
Surface Layers ◽  
Outer Cortex ◽  
Electron Microscopic ◽  
Intact Cells ◽  
Morphological Aspects ◽  
Microscopic Studies

Epidermal cells and cells originating in the outer cortex form the surface layers of peanut (Arachis hypogaea) roots, the outermost of which separate and shed from the periphery. Shedding takes place continuously and over the whole surface of the root. Light and electron microscopic studies revealed that the shedding of surface layers involves modification of cell walls and separation of intact cells. Wall breakdown, as well as the expansion of cells resulting from wall breakdown, might facilitate the separation of intact cells. Examination of enzymes revealed that cellulase showed much higher activity in the shedding layers than in the remaining tissues. The results suggest that the cell separation process in peanut roots involves a wall-degrading enzyme-mediated mechanism. Key words: Arachis hypogaea, morphology, root, shedding, surface layers, wall breakdown.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

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