scholarly journals Beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats

2012 ◽  
Vol 109 (10) ◽  
pp. 1806-1812 ◽  
Author(s):  
Nallasamy Palanisamy ◽  
Anuradha C. Venkataraman
Keyword(s):  
Blood Pressure ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Hypertensive Rats ◽  
Enos Expression ◽  
Electron Microscopic ◽  
Plasma Angiotensin ◽  
Enhanced Expression ◽  
Male Wistar Rats ◽  
Microscopic Studies

The study evaluates the effects of genistein on blood pressure (BP) and ultrastructural changes in kidney of fructose-fed hypertensive rats. Male Wistar rats were fed a diet containing 60 % starch or 60 % fructose as the source of carbohydrate. After 15 d, rats in each dietary group were divided into two groups and were treated with either genistein (1 mg/kg per d) in dimethylsulfoxide (DMSO) or 30 % DMSO alone. BP, pressor mechanisms, protein kinase C-βII (PKC-βII) expression, endothelial NO synthase (eNOS) expression and renal ultrastructural changes were evaluated after 60 d. Fructose-fed rats displayed significant elevation in BP and heart rate. Significant increase in plasma angiotensin-converting enzyme (ACE) activity, alterations in renal lipid profile, nitrite and kallikrein activity, enhanced expression of membrane-associated PKC-βII and decreased expression of eNOS were observed in them. Histology and electron microscopic studies showed structural changes in the kidney. Genistein administration lowered BP, restored ACE, PKC-βII and eNOS expression and preserved renal ultrastructural integrity. These findings demonstrate that genistein has effects on eNOS activity in renal cells, leading to eNOS activation and NO synthesis. These effects could have been mediated by activation of PKC-βII. The observed benefits of genistein make it a promising candidate for therapy of diabetic kidney disease.

Fine Structural Changes of the Porcine Oviductal Epithelium During Late and Term Pregnancy

1976 ◽  
Vol 34 ◽  
pp. 150-151
Author(s):  
R. K. Nayak ◽  
E. N. Albert
Keyword(s):  
Estrous Cycle ◽  
Early Pregnancy ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Secretory Cells ◽  
Corpora Lutea ◽  
Term Pregnancy ◽  
Electron Microscopic ◽  
Uterine Tube ◽  
Microscopic Studies

The characteristic ultrastructural changes in the ciliated and secretory cells of the tubal epithelium of adult gilts during the estrous cycle have been described (1). Earlier light microscopic studies revealed that pregnancy inhibited the cyclic alterations in the tubes of the sow and the epithelium appeared to remain in the state which was found in the second week after ovulation, when the corpora lutea were full grown (2). Nayak and Zimmerman have presented limited electron microscopic studies of the porcine tubal epithelium during early pregnancy (3) . To the best of our knowledge, no electron microscopic studies of the porcine uterine tube epithelium during late and term pregnancy have been reported.

HISTOMETRIC AND ULTRASTRUCTURAL ORGANIZATION OF THE NERVIMUSCULAR TERMINALS OF SKELETAL MUSCLES AT A HYPOKINESIA

2019 ◽  
pp. 55-63
Author(s):  
Z. M. Yaschyshyn ◽  
S. L. Popel
Keyword(s):  
Skeletal Muscles ◽  
Structural Adjustment ◽  
Muscle Fibers ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Myelinated Nerve ◽  
Male Wistar Rats

The aim: to study the dynamics of histological and ultrastructural changes in muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia at different stages of ontogenesis. Methods. Studied skeletal muscles and their peripheral nervous apparatus of laboratory male Wistar rats aged 30 to 270 days. The restriction of motor activity was carried out in special canister cells for 30, 60, 90, and 240 days (5 animals for each term). To determine the type of muscle fiber, the Nahlas histochemical method was used, the Kulchitsky method was used to detect myelinated nerve fibers, the Bilshovsky-Gros method and the electron microscopic method to identify neuromuscular endings. Results. The data of histological and electron microscopic examination of skeletal muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia indicate their regular restructuring during the development of muscles, the formation of their synapses and structures that are associated with them at different stages of ontogenesis. Conclusion. The study provides an in-depth understanding of the relative frequency and nature of the disturbance of the neuromuscular endings during prolonged hypokinesia and its effect on the dynamics of structural adjustment of individual types of muscle fibers in ontogenesis.

HISTOMETRIC AND ULTRASTRUCTURAL ORGANIZATION OF THE NERVIMUSCULAR TERMINALS OF SKELETAL MUSCLES AT A HYPOKINESIA

2019 ◽  
pp. 55-63
Author(s):  
Z. M. Yaschyshyn ◽  
S. L. Popel
Keyword(s):  
Skeletal Muscles ◽  
Structural Adjustment ◽  
Muscle Fibers ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Myelinated Nerve ◽  
Male Wistar Rats

The aim: to study the dynamics of histological and ultrastructural changes in muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia at different stages of ontogenesis. Methods. Studied skeletal muscles and their peripheral nervous apparatus of laboratory male Wistar rats aged 30 to 270 days. The restriction of motor activity was carried out in special canister cells for 30, 60, 90, and 240 days (5 animals for each term). To determine the type of muscle fiber, the Nahlas histochemical method was used, the Kulchitsky method was used to detect myelinated nerve fibers, the Bilshovsky-Gros method and the electron microscopic method to identify neuromuscular endings. Results. The data of histological and electron microscopic examination of skeletal muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia indicate their regular restructuring during the development of muscles, the formation of their synapses and structures that are associated with them at different stages of ontogenesis. Conclusion. The study provides an in-depth understanding of the relative frequency and nature of the disturbance of the neuromuscular endings during prolonged hypokinesia and its effect on the dynamics of structural adjustment of individual types of muscle fibers in ontogenesis.

Effects of Combination Chemical Peel on Facial Photodamaged Skin

1998 ◽  
Vol 15 (3) ◽  
pp. 263-271 ◽  
Author(s):  
May H. El Samahy ◽  
Mohamed M. Ghoz ◽  
Naglaa Ramzy
Keyword(s):  
Glycolic Acid ◽  
Electron Microscopic Examination ◽  
Ultrastructural Changes ◽  
Elastic Fibers ◽  
Electron Microscopic ◽  
Trichloracetic Acid ◽  
Age Related ◽  
Photodamaged Skin ◽  
Microscopic Studies ◽  
Activated Fibroblasts

Introduction: Chemical peeling involves the topical application of a wounding agent with the goal of effecting an organized regeneration of the skin. The histologic and ultrastructural features of actinic and age-related damage include structural abnormalities that disrupt normal epidermal and dermal architecture. The purpose of the present study is to evaluate the clinical and histologic effects of an enhanced medium-depth peel on photodamaged skin. We aimed to correlate the clinical and histologic findings with the ultrastructural changes occurring after the peel. These ultrastructural features are supposed to be more precise and informative than the clinical or histological response. They may also be employed as markers of peel response. Materials and Methods: In the present study, five patients with actinically damaged skin underwent enhanced medium-depth peels using 70% glycolic acid and 35% trichloracetic acid. Biopsy specimens were taken before the peel and 3 months after the peel for histologic and electron microscopic examination. Results: Clinical resolution of actinic damage corresponded with restoration of epidermal polarity. Characteristic histologic and ultrastructural features of the skin after peeling include markedly decreased epidermal intracytoplasmic vacuoles, decreased elastic fibers, increased activated fibroblasts, and organized parallel arrays of collagen fibrils. The diameters of individual fibrils are consistent with recent production of collagen by activated fibroblasts. Conclusion: Glycolic acid—tricholoro-acetic acid (GA-TCA) is an effective combination for a medium-depth peel in photodamaged skin both clinically and histologically. Electron microscopic studies following medium-depth peels reveal changes more profound than those seen histologically. The characteristic changes occurring in the keratinocytes, collagen, and elastic fibrils may be considered as guidelines or markers of the peel response.

Ultrastructural Changes in Euglena After Ultraviolet Irradiation

1973 ◽  
Vol 13 (3) ◽  
pp. 799-809
Author(s):  
A. MICHAELS ◽  
A. GIBOR
Keyword(s):  
Ultraviolet Light ◽  
Ultraviolet Irradiation ◽  
Euglena Gracilis ◽  
Structural Changes ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Bleaching Process ◽  
In Cells

The structural changes associated with the ultraviolet-induced bleaching of light-grown cells of Euglena gracilis were investigated. Our light- and electron-microscopic observations of the bleaching process indicate that there is a continuity of plastid structure in cells 5 generations after receiving a bleaching dose of ultraviolet light. There seems to be a continuous dilution of the plastid thylakoids and a decrease in plastid size in the bleaching cells. There also seems to be a change in the position of the plastids in relation to the mitochondria in the bleaching cells. The plastids and possibly the mitochondria are the only organelles which are affected by the ultraviolet irradiation. The continuity of plastids in bleaching cells of Euglena is discussed in relation to the proposed effect of the ultraviolet light.

Sardine proteins (Sardina pilchardus) combined with green lemon zest (Citrus latifolia) improve blood pressure, lipid profile and redox status in diabetic hypertensive rats

2018 ◽  
Vol 48 (4) ◽  
pp. 654-668 ◽  
Author(s):  
HadjMostefa Khelladi ◽  
Djamil Krouf ◽  
Nawal Taleb-Dida
Keyword(s):  
Blood Pressure ◽  
Redox Status ◽  
Cardiovascular Complications ◽  
Reduce Blood Pressure ◽  
Diabetic Patients ◽  
Hypertensive Rats ◽  
Content Type ◽  
Protein Group ◽  
Male Wistar Rats ◽  
Tbars Values

Purpose This paper aims to study the effect of green lemon zest combined with sardine proteins in diabetic hypertensive rats (DHRs). Design/methodology/approach Male Wistar rats (n = 30) weighing 250 ± 10 g were divided into five groups. The first group consumed a diet containing 20 per cent casein (C). The other four groups are rendered diabetic by intraperitoneal injection of streptozotocin (40 mg/kg body weight), then hypertensive by subcutaneous implantation controlled time-release pellet containing ouabain (0.25 mg/pellet). One untreated group (DHR) consumed 20 per cent casein and the three other groups consumed the same diet supplemented with 2 per cent green lemon zest (DHR-lz), or with 20 per cent of sardine protein (group DHR-sp) or with the combination of both sardine proteins and green lemon zest (group DHR-sp + lz). Findings DHRs feeding on the combination of both sardine protein (sp) and lemon zest (lz) induced a significant decrease of diastolic blood pressure and heart rates values compared with DHR (p < 0.05). The HDLC values were increased by +55 per cent in DHR-sp + lz compared with DHR group. Moreover, plasma non-HDLC concentrations were decreased significantly compared to DHR, DHR-lz, DHR-sp and C groups. In DHR-sp + lzvs DHR group, TBARS values were decreased by −25 per cent in the liver. Moreover, kidney TBARS were significantly reduced by −66, −51, −65 and −67 per cent compared with C, DHR, DHR-lz and DHR-sp, respectively. Originality/value These results suggest that consumption of green lemon zest combined with sardine proteins can reduce blood pressure and tissue oxidative damage and, therefore, help to prevent cardiovascular complications in hypertensive diabetic patients.

Primary versus secondary structural changes of the blood vessels in hypertension

1985 ◽  
Vol 63 (4) ◽  
pp. 392-401 ◽  
Author(s):  
Robert M. K. W. Lee ◽  
John S. Smeda
Keyword(s):  
Blood Pressure ◽  
Blood Vessels ◽  
Structural Changes ◽  
Vascular Wall ◽  
Wall Thickening ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto ◽  
Pressure Independent ◽  
Hypertension Development

Various researchers have hypothesized that the thickening of the vascular wall plays an important role in the maintenance of hypertension. Such an alteration can increase the vascular resistance by exerting two effects. A thickened vascular wall could occlude the lumen of the blood vessel and (or) cause the artery to hyperreact to contractile stimuli. Until recently, it has been a general conclusion that such alterations were a secondary adaptation produced by the elevation of blood pressure. Consistent with this view, certain classes of larger arteries do exhibit a thickened vascular wall late during hypertension development and such changes can be prevented from occurring by antihypertensive treatment. However, recent studies involving the mesenteric and renal arteries of Wistar-Kyoto spontaneously hypertensive rats have shown that wall thickening of the vasculature occurs prior to hypertension development and is present even under conditions where the blood pressure has been normalized throughout the animal's life. These latter observations suggest that some structural alterations in the blood vessels observed in hypertension are pressure independent and could be of etiological importance in the initiation of hypertension.

scholarly journals The Morphological Assessment of Rats` Liver after the Introduction of the Nucleoside Reverse Transcriptase Inhibitor of Azidothymidine

2019 ◽  
pp. 61-67
Author(s):  
M. N. Kurbat ◽  
R. I. Kravchuk ◽  
O. B. Ostrovskaya
Keyword(s):  
Nucleoside Reverse Transcriptase Inhibitor ◽  
Structural Changes ◽  
Electron Microscopic Examination ◽  
Transcriptase Inhibitor ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Structural And Functional Properties ◽  
Hepatotoxic Effect ◽  
Reverse Transcriptase Inhibitor ◽  
Morphological Assessment

Objective: to study the microscopic and ultrastructural changes in the liver of rats exposed to AZT. Material and methods. The histological and electron microscopic examination of the liver samples with mitochondrial morphometry has been performed. Results . The 7-day administration of AZT does not cause any significant structural changes in the liver. The exposure to the drug for 21 days leads to the development of moderate inflammatory and degenerative processes in the liver, including changes in the structure of hepatocyte mitochondria. Conclusion. One of the pathogenetic mechanisms of the hepatotoxic effect of AZT is its impact on the structural and functional properties of hepatocyte mitochondria.

scholarly journals Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

2016 ◽  
Vol 18 (1) ◽  
pp. 113-124 ◽  
Author(s):  
G.H. CUNHA ◽  
F.V. FECHINE ◽  
F.A. FROTA BEZERRA ◽  
M.O. MORAES ◽  
E.R. SILVEIRA ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vascular Reactivity ◽  
Antihypertensive Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Hypertensive Rats ◽  
Male Wistar Rats ◽  
Main Components

ABSTRACT The aim of this study was to characterize components of the EOAz and its hexane (HFEOAz), chloroform (CFEOAz) and methanol (MFEOAz) fractions, and its antihypertensive effect. EOAz was extracted from leaves by hydrodistillation. Aliquot was subjected to selective desorption with silica gel column and eluted with hexane, chloroform and methanol. The components of the EOAz and fractions were analyzed by gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy of hydrogen. Experiments of vascular reactivity were performed with isolated aortic rings of male Wistar rats. Antihypertensive effect was evaluated in hypertensive rats submitted to the inhibition of synthesis of nitric oxide. Blood pressure was measured indirectly by tail plethysmography. MFEOAz showed the lowest EC50 (150.45 µg/mL), 1,8-cineole (27.81%) and terpinen-4-ol (57.35%) as main components. Single administration by nasogastric tube of EOAz, fractions and captopril significantly reduced the blood pressure of hypertensive rats, when compared to animals of the negative control group with distilled water. In conclusion, the potency of the MFEOAz was higher than that of EOAz and other fractions. The antihypertensive effect of EOAz and fractions was similar, higher than the negative control and lower than that of captopril.

Sign in / Sign up

Export Citation Format

Share Document