Fixation artifacts in transmission electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 336-337
Author(s):  
Blair Bowers
Keyword(s):  
Single Cells ◽  
Preparative Method ◽  
High Resolution Imaging ◽  
Fixation Light ◽  
Crucial Step ◽  
Light Microscopic Observation ◽  
Transmission Electron ◽  
Before And After ◽  
Resolution Imaging ◽  
Very High

Cell and tissue preparations for transmission EM contain artifacts introduced at each step of the preparative method. Fixation, as a first and crucial step, is subject to both gross and subtle artifact. For familiar tissues, gross artifacts are easily recognized. Artifacts in unfamiliar cells and subtle distortions or alterations of structure or localizations of molecules of interest are more difficult to assess.Three general approaches are useful for identification of fixation artifacts. Especially in the case of single cells, it is possible to make direct comparison of living and fixed specimens. Advanced light microscopic methods allow very high-resolution imaging of cells and comparison of structure before and after fixation. Light microscopic observation during fixation has documented examples both of creation of structure and disappearance of structure. A more widely applicable approach is the comparison of structure in the same tissue after fixation by different procedures.

scholarly journals μCB-seq: Microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

2020 ◽  
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi Zalavadia ◽  
Aaron M. Streets
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Protein Localization ◽  
Single Cells ◽  
Optical Measurements ◽  
Detection Sensitivity ◽  
High Resolution Imaging ◽  
Cell Analysis ◽  
Resolution Imaging

AbstractSingle-cell RNA sequencing (scRNA-seq) enables the investigation of complex biological processes in multicellular organisms with high resolution. However, many phenotypic features that are critical to understanding the functional role of cells in a heterogeneous tissue or organ are not directly encoded in the genome and therefore cannot be profiled with scRNA-seq. Quantitative optical microscopy has long been a powerful approach for characterizing diverse cellular phenotypes including cell morphology, protein localization, and chemical composition. Combining scRNA-seq with optical imaging has the potential to provide comprehensive single-cell analysis, allowing for functional integration of gene expression profiling and cell-state characterization. However, it is difficult to track single cells through both measurements; therefore, coupling current scRNA-seq protocols with optical measurements remains a challenge. Here, we report Microfluidic Cell Barcoding and Sequencing (μCB-seq), a microfluidic platform that combines high-resolution imaging and sequencing of single cells. μCB-seq is enabled by a novel fabrication method that preloads primers with known barcode sequences inside addressable reaction chambers of a microfluidic device. In addition to enabling multi-modal single-cell analysis, μCB-seq improves gene detection sensitivity, providing a scalable and accurate method for information-rich characterization of single cells.

High Resolution Imaging of As-Grown Sapphire Surfaces

1985 ◽  
Vol 62 ◽  
Author(s):  
Tung Hsu ◽  
S. R. Nutt
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Structural Features ◽  
High Resolution Imaging ◽  
Sapphire Surface ◽  
Surface Steps ◽  
Transmission Electron ◽  
Reflection Electron Microscopy ◽  
Resolution Imaging ◽  
Surface Facets

ABSTRACTSurfaces of commercially grown edge-defined film-fed growth sapphire (EFG α-Al2O3) were studied in the electron microscope using both reflection electron microscopy (REM) and conventional transmission electron microscopy (TEM). The as-grown sapphire surface, ostensibly {1120}, was characterized by “rooftop” structures which were often locally periodic. These rooftop structures consisted of alternating {1120} facets and additional facets inclined a few degrees. The crystallography of the surface facets was analyzed using REM imaging of bulk specimens, and trace analysis of back-thinned plan section TEM specimens. Surface roughness was measured by stylus profilometry. and these measurements were compared to the electron microscopy observations. Fine structural features parallel to <0110> directions were also observed in both REM and TEM experiments, and these were attributed to surface steps of atomic scales.

scholarly journals Recent Advances in Transmission Electron Microscopy for Materials Science at the EMAT Lab of the University of Antwerp

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1304 ◽  
Author(s):  
Giulio Guzzinati ◽  
Thomas Altantzis ◽  
Maria Batuk ◽  
Annick De Backer ◽  
Gunnar Lumbeeck ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Materials Science ◽  
Electron Tomography ◽  
High Resolution Imaging ◽  
Rapid Progress ◽  
Transmission Electron ◽  
The World ◽  
Resolution Imaging ◽  
The University

The rapid progress in materials science that enables the design of materials down to the nanoscale also demands characterization techniques able to analyze the materials down to the same scale, such as transmission electron microscopy. As Belgium’s foremost electron microscopy group, among the largest in the world, EMAT is continuously contributing to the development of TEM techniques, such as high-resolution imaging, diffraction, electron tomography, and spectroscopies, with an emphasis on quantification and reproducibility, as well as employing TEM methodology at the highest level to solve real-world materials science problems. The lab’s recent contributions are presented here together with specific case studies in order to highlight the usefulness of TEM to the advancement of materials science.

scholarly journals High Resolution Imaging Forty Years Ago

1994 ◽  
Vol 158 ◽  
pp. 337-341
Author(s):  
R. C. Jennison
Keyword(s):  
High Resolution ◽  
Point Sources ◽  
Resolving Power ◽  
High Angular Resolution ◽  
Electromagnetic Spectrum ◽  
High Resolution Imaging ◽  
Resolution Imaging ◽  
Optical Telescopes ◽  
Very High ◽  
Better Than

This conference is concerned with the very high resolution imaging of cosmic sources in many parts of the electromagnetic spectrum. Various techniques are now available and the equipment is often automated and highly sophisticated but the term ‘very high angular resolution’ is comparative. Many of the problems existed over forty years ago when the best resolving power was about half a degree and the two major radio ‘stars’ appeared to be point sources. Very high resolution imaging in those days was the struggle to reach one minute of arc and Hanbury Brown had set his sights on considerably better than one second of arc with the concept of the intensity interferometer. The dream was to achieve a resolving power comparable to that of optical telescopes.

scholarly journals Introduction to the Joint Commission Meeting on High Resolution Imaging from the Ground

1989 ◽  
Vol 8 ◽  
pp. 545-546
Author(s):  
John Davis
Keyword(s):  
High Resolution ◽  
Angular Resolution ◽  
High Angular Resolution ◽  
High Resolution Imaging ◽  
Wide Range ◽  
High Resolution Images ◽  
Resolution Imaging ◽  
Optical Wavelengths ◽  
To Come ◽  
Very High

As a result of advances in instrumentation and techniques, from radio through to optical wavelengths, we have before us the prospect of producing very high resolution images of a wide range of objects across this entire spectral range. This prospect, and the new knowledge and discoveries that may be anticipated from it, lie behind an upsurge in interest in high resolution imaging from the ground. Several new high angular resolution instruments for radio, infrared, and optical wavelengths are expected to come into operation before the 1991 IAU General Assembly.

scholarly journals Evolution of INO Uncooled Infrared Cameras Towards Very High Resolution Imaging

2011 ◽  
Vol 276 ◽  
pp. 012114 ◽  
Author(s):  
Alain Bergeron ◽  
Hubert Jerominek ◽  
Claude Chevalier ◽  
Loïc Le Noc ◽  
Bruno Tremblay ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Resolution Imaging ◽  
Infrared Cameras ◽  
Very High
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