Localization of Adenosine Triphosphatase Activity in the Phleom of Nicotiana Tabacum

1972 ◽  
Vol 30 ◽  
pp. 230-231
Author(s):  
James Cronshaw ◽  
Jamison E. Gilder
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Higher Plants ◽  
High Surface ◽  
Root Tip ◽  
Animal Cells ◽  
Physiological Processes ◽  
Tip Cells ◽  
Atpase Localization

Adenosine triphosphatase (ATPase) activity has been shown to be associated with numerous physiological processes in both plants and animal cells. Biochemical studies have shown that in higher plants ATPase activity is high in cell wall preparations and is associated with the plasma membrane, nuclei, mitochondria, chloroplasts and lysosomes. However, there have been only a few ATPase localization studies of higher plants at the electron microscope level. Poux (1967) demonstrated ATPase activity associated with most cellular organelles in the protoderm cells of Cucumis roots. Hall (1971) has demonstrated ATPase activity in root tip cells of Zea mays. There was high surface activity largely associated with the plasma membrane and plasmodesmata. ATPase activity was also demonstrated in mitochondria, dictyosomes, endoplasmic reticulum and plastids.

scholarly journals Evidence that Mg2+- or Ca2+-activated adenosine triphosphatase in rat pancreas is a plasma-membrane ecto-enzyme

1983 ◽  
Vol 214 (1) ◽  
pp. 59-68 ◽  
Author(s):  
J M Hamlyn ◽  
A E Senior
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Active Site ◽  
Adenosine Triphosphatase ◽  
Hydrolytic Activity ◽  
Intact Cells ◽  
Rat Pancreas ◽  
Pancreatic Cells ◽  
Nucleoside Triphosphates ◽  
Specific Inhibitors

Preparations of enzymically dispersed rat pancreatic cells hydrolyse externally added nucleoside triphosphates and diphosphates at high rates in the presence of Mg2+ or Ca2+. The lack of response to specific inhibitors and activators differentiates this hydrolytic activity from that of other well-characterized ion-transporting ATPases. Studies based on inactivation of this hydrolytic activity by the covalently reacting, slowly permeating probe diazotized sulphanilic acid indicated that this nucleoside tri- and di-phosphatase is primarily a plasma-membrane ecto-enzyme. It is the major ATPase activity associated with intact cells, homogenates and isolated plasma-membrane fractions. Concanavalin A stimulates this ATPase activity of intact cells and isolated plasma-membrane fractions. The insensitivity of this ATPase activity to univalent ions and inhibitors of pancreatic electrolyte secretion, taken together with the evidence that the active site is externally located, suggests that this enzyme is not directly involved in HCO3- secretion in the pancreas. Its actual function remains unknown.

scholarly journals LEAD ION AND PHOSPHATASE HISTOCHEMISTRY II. EFFECT OF ADENOSINE TRIPHOSPHATE HYDROLYSIS BY LEAD ION ON THE HISTOCHEMICAL LOCALIZATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY

1966 ◽  
Vol 14 (10) ◽  
pp. 702-710 ◽  
Author(s):  
HAROLD L. MOSES ◽  
ALAN S. ROSENTHAL ◽  
DAVID L. BEAVER ◽  
SHIRLEY S. SCHUFFMAN
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Lead Nitrate ◽  
Histochemical Localization ◽  
Lead Ion ◽  
Membrane Localization ◽  
Fixed Tissue ◽  
Plasma Membrane Localization ◽  
Hydrolysis Of

The lead method of Wachstein and Meisel for the histochemical localization of adenosine triphosphatase (ATPase) involves the incubation of sections of fixed tissue in reaction mixtures containing ATP, lead nitrate, magnesium sulfate and a Tris-maleate buffer, pH 7.2. Both fixation and the presence of lead ion were shown to inhibit tissue ATPase activity markedly and to inactivate the sodium- plus potassium-dependent membrane ATPase. In addition, recent studies have demonstrated that lead ion, in the concentration used in the Wachstein-Meisel system, will catalyze the hydrolysis of ATP. Studies on the effect of this nonenzymatic reaction on the histochemical localization of ATPases demonstrated that plasma membrane localization occurred only with lead and ATP concentrations which gave significant nonenzymatic hydrolysis of ATP by lead. In addition, nuclear and mitochondrial localization without accompanying plasma membrane localization could be obtained in formalin-fixed tissue with decreased concentrations of lead or with increased concentrations of ATP in the reaction mixture. The amount of lead-catalyzed hydrolysis was in the same order of magnitude as fixed tissue ATPase activity and could quantitatively account for the amount of phosphate needed to give recognizable localization of lead salt deposits in sections of fixed tissue.

scholarly journals Homology bioinformatics analysis of IPA1 gene in higher plants

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Zhongling Gong
Keyword(s):  
Phylogenetic Tree ◽  
Higher Plants ◽  
Water Extract ◽  
Root Tip ◽  
Chromosome Fragment ◽  
Tree Construction ◽  
Chromosome Bridge ◽  
Support Rate ◽  
Tip Cells ◽  
Root Tip Cells

IPA1 gene controlled paddy has increased in height, sturdy stem, number of tillers decreased, grains per panicle and dry grains weight increased significantly. In this study, the homology of IPA1 gene in 26 types of plant from 15 families was analyzed, and thus constructed its phylogenetic tree. The results of phylogenetic tree construction based on its amino acid sequence showed that the species from the same family, such as Gramineae, Rosaceae and Palmaceae, were well clustered for different branches and had high support rates. Furthermore, we constructed the phylogenetic tree based on its CDS sequence, and the species of Gramineae was well clustered, and the support rate was 100%. Our results indicate that IPA1 has high homology in higher class of plants, especially in the Gramineae, which is of great significance for us to further study the yield of rice and other gramineous species.s with time and concentration effect (3) Achilla glauca extract could make the chromosome bridge, chromosome fragment, chromosome lag in the root tip cells of Vicia faba and increase in micronucleus rate. The results showed that the water extract of Solidago canadensis had different degree of inhibition and damage to silkworm root tip cells; it also had some genetic toxicity and rapid diffusion ability.

The plasma membrane intactness of root-tip cells is a primary factor for Al-tolerance in cultivars of five species

2001 ◽  
Vol 47 (3) ◽  
pp. 489-501 ◽  
Author(s):  
Satoru Ishikawa ◽  
Tadao Wagatsuma ◽  
Tsuyoshi Takano ◽  
Keitaro Tawaraya ◽  
Koji Oomata
Keyword(s):  
Plasma Membrane ◽  
Primary Factor ◽  
Root Tip ◽  
Al Tolerance ◽  
Tip Cells ◽  
Root Tip Cells

Relative abundance of Δ5-sterols in plasma membrane lipids of root-tip cells correlates with aluminum tolerance of rice

2009 ◽  
Vol 135 (1) ◽  
pp. 73-83 ◽  
Author(s):  
M. Shahadat Hossain Khan ◽  
Keitarou Tawaraya ◽  
Hiroshi Sekimoto ◽  
Hiroyuki Koyama ◽  
Yuriko Kobayashi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Relative Abundance ◽  
Membrane Lipids ◽  
Aluminum Tolerance ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

scholarly journals AN ATYPICAL CRISTA RESEMBLING A "TIGHT JUNCTION" IN BEAN ROOT MITOCHONDRIA

1968 ◽  
Vol 39 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Eldon H. Newcomb ◽  
Martin W. Steer ◽  
Peter K. Hepler ◽  
William P. Wergin
Keyword(s):  
Phaseolus Vulgaris ◽  
Tight Junction ◽  
Tight Junctions ◽  
Osmium Tetroxide ◽  
Root Tip ◽  
Mitochondrial Matrix ◽  
Phaseolus Vulgaris L ◽  
Animal Cells ◽  
Tip Cells ◽  
Root Tip Cells

The conformation and structure of an atypical crista found in a small percentage of the mitochondria in root tip cells of Phaseolus vulgaris L. have been studied electron microscopically in material fixed in glutaraldehyde followed by osmium tetroxide. In its transformation into an atypical crista, a normal crista elongates, broadens, and flattens, and the inner leaflets of its apposed unit membranes appear to fuse in a manner analogous to the formation of "tight junctions" between certain animal cells. The result is a large platelike, quintuple-layered structure, 240–260 A thick, whose long axis parallels that of the mitochondrion. The outer layers of the "plate," bordering on the mitochondrial matrix, are thickened and exhibit striking patterns in the micrographs. The structure of the plate is compared with that previously described for tight junctions between animal cells.

scholarly journals Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 150 ◽  
Author(s):  
Nora A. Gutiérrez-Nájera ◽  
Mariana Saucedo-García ◽  
Liliana Noyola-Martínez ◽  
Christian Vázquez-Vázquez ◽  
Silvia Palacios-Bahena ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Fold Increase ◽  
Membrane Properties ◽  
Ceramide Synthase ◽  
Animal Cells ◽  
Plasma Membrane Fluidity ◽  
Maize Embryos

Fumonisin B1 is a mycotoxin produced by Fusarium verticillioides that modifies the membrane properties from animal cells and inhibits complex sphingolipids synthesis through the inhibition of ceramide synthase. The aim of this work was to determine the effect of Fumonisin B1 on the plant plasma membrane when the mycotoxin was added to germinating maize embryos. Fumonisin B1 addition to the embryos diminished plasma membrane fluidity, increased electrolyte leakage, caused a 7-fold increase of sphinganine and a small decrease in glucosylceramide in the plasma membrane, without affecting phytosphingosine levels or fatty acid composition. A 20%–30% inhibition of the plasma membrane H+-ATPase activity was observed when embryos were germinated in the presence of the mycotoxin. Such inhibition was only associated to the decrease in glucosylceramide and the addition of exogenous ceramide to the embryos relieved the inhibition of Fumonisin B1. These results indicate that exposure of the maize embryos for 24 h to Fumonisin B1 allowed the mycotoxin to target ceramide synthase at the endoplasmic reticulum, eliciting an imbalance of endogenous sphingolipids. The latter disrupted membrane properties and inhibited the plasma membrane H+-ATPase activity. Altogether, these results illustrate the mode of action of the pathogen and a plant defense strategy.

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