Ultrastructure of two neoplasms in culture compared with original biopsies

1984 ◽  
Vol 42 ◽  
pp. 50-51
Author(s):  
Joseph M. Harb ◽  
James T. Casper ◽  
Vlcki Piaskowski
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Biopsy Specimen ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells ◽  
Morphological Distinction ◽  
Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

Phase-contrast and electron-microscopic observations on a membranous complex in primary spermatocytes of the mouse

1975 ◽  
Vol 18 (1) ◽  
pp. 1-17
Author(s):  
A. Pleshkewych ◽  
L. Levine
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Phase Contrast ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Cytoplasmic Inclusion ◽  
Electron Microscopic ◽  
Secondary Spermatocyte ◽  
Living Mouse ◽  
Circular Contour

A prominent cytoplasmic inclusion present in living mouse primary spermatocytes has been observed by both light and electron microscopy. It began to form at prometaphase and continued to increase in thickness and length as the cells developed. By metaphase it was a distinct sausage-shaped boundary that enclosed a portion of the cytoplasm between the spindle and the cell membrane. At the end of metaphase, the inclusion reached its maximum length. At telophase, it was divided between the daughter secondaries. The inclusion persisted as a circular contour in the interphase secondary spermatocyte. Electron microscopy of the same cultured cells that were previously observed with light microscopy revealed that the inclusion was a distinctive formation of membranes. It consisted of agranular cisternae and vesicles, and was therefore a membranous complex. Many of the smaller vesicles in the membranous complex resembled those found in the spindle. The cisternae in the membranous complex were identical to the cisternal endoplasmic reticulum of interphase primary spermatocytes. Nevertheless, the organization of vesicles and cisternae into the membranous complex was unique for the primaries in division stages, since such an organization was not present in their interphase stages.

scholarly journals THE INDUCTION OF MELANIZATION IN GOLDFISH SCALES WITH ACTH, IN VITRO

1963 ◽  
Vol 18 (1) ◽  
pp. 181-194 ◽  
Author(s):  
Alden V. Loud ◽  
Yutaka Mishima
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Silver Nitrate ◽  
Light And Electron Microscopy ◽  
Subcellular Structure ◽  
Acth Stimulation ◽  
Cytoplasmic Bodies ◽  
Silver Nitrate Staining ◽  
Stimulation Of

The induction of melanization in xanthic goldfish scales with ACTH in vitro has been studied by light and electron microscopy utilizing ammoniated silver nitrate staining of premelanin and melanin. The melanized cells (melanophores and melanocytes) and the yellow pigmented cells (lipophores and the newly described lipocytes) were found to possess many similarities at the levels of cellular and subcellular structure. The latter cells contain characteristic cytoplasmic bodies which react positively to the premelanin stain. Changes accompanying ACTH stimulation of goldfish scales in tissue culture suggest that these bodies in the lipocytes and lipophores can become melanized. Electron micrographs illustrate the intermediate staining of newly formed melanin granules in an induced melanocyte and the appearance of a transitional melanolipophore. It is postulated that ACTH can promote the association of the enzyme tyrosinase with the preformed structure of unmelanized granules.

scholarly journals 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

2016 ◽  
Vol 130 (1) ◽  
pp. 278-291 ◽  
Author(s):  
Matthew R. G. Russell ◽  
Thomas R. Lerner ◽  
Jemima J. Burden ◽  
David O. Nkwe ◽  
Annegret Pelchen-Matthews ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Scanning Electron

scholarly journals Ultrastructure of double minutes from a human tumor cell line.

1979 ◽  
Vol 83 (3) ◽  
pp. 663-666 ◽  
Author(s):  
P E Barker ◽  
E Stubblefield
Keyword(s):  
Electron Microscopy ◽  
Chemical Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
Double Minutes

Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.

ECHO 9 virus in tissue culture observed by light and electron microscopy

Virology ◽  
1960 ◽  
Vol 12 (2) ◽  
pp. 331-334 ◽  
Author(s):  
R.A. Rifkind ◽  
G.C. Godman ◽  
C. Howe ◽  
C. Morgan ◽  
H.M. Rose
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light And Electron Microscopy

Cloning of human tumor cells in soft agar

1979 ◽  
Vol 5 (3) ◽  
pp. 1141-1145 ◽  
Author(s):  
Anne W. Hamburger
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells

scholarly journals Epinemin: a new protein associated with vimentin filaments in non-neural cells.

1983 ◽  
Vol 97 (6) ◽  
pp. 1891-1905 ◽  
Author(s):  
D Lawson
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Neural Cells ◽  
Fingerprint Pattern ◽  
Low Salt ◽  
Nonionic Detergent ◽  
New Protein ◽  
Vimentin Filaments

In this report I describe a new protein, defined by a monoclonal antibody, which is associated with vimentin filaments in a variety of cultured cells and in skeletal muscle. By immunofluorescence it is absent in smooth muscle, in cells without vimentin, and in neural vimentin containing cells. This protein has a molecular weight of 44,500, a pl of 5, a two-dimensional tryptic peptide fingerprint pattern different from vimentin, is unrelated to actin by Cleveland peptide analysis and by light and electron microscopy, and is not recognized by either a polyclonal antivimentin antibody (Frank, E.D., and L. Warren, 1981, Proc. Natl. Acad. Sci. USA, 78:3020-3024) or a monoclonal antibody against all classes of intermediate filaments (Pruss, R.M., R. Mirsky, M.C. Raff, R. Thorpe, A.J. Dowding, and B.H. Anderton, 1981, Cell, 27:419-428). The protein is resistant to nonionic detergent extraction, is soluble in high salt and can thus be removed from vimentin filaments, but fragments with vimentin in either low salt or anionic detergent and collapses with vimentin in colchicine-treated cells. By light microscopy, the distribution of the protein is indistinguishable from vimentin filaments and appears uniform along them. In contrast, immunoferritin electron microscopy reveals that the molecule is distributed in an intermittent pattern on vimentin filaments. Adopting the terminology of Granger and Lazarides (1980, Cell, 30:263-275), the molecule is called epinemin, meaning "upon filaments."

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