Electron Microscopic study of the parathyroid gland of the melatonin-treated hamster

1990 ◽  
Vol 48 (3) ◽  
pp. 328-329
Author(s):  
Shizuko Shoumura ◽  
Shoichi Emura ◽  
Tomo Yamahira ◽  
Tomoo Kawada ◽  
Hiroshi Oda ◽  
...  
Keyword(s):  
Body Weight ◽  
Pineal Gland ◽  
Parathyroid Gland ◽  
Morphological Changes ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Golden Hamsters ◽  
Microscopic Studies ◽  
The Relationship ◽  
Average Body

Some studies have dealt with the relationship between the pineal gland and the parathyroid gland (PTG) Morphological changes suggest that the pineal gland may inhibit or stimulate the PTG. There are a few light microscopic studies on the effects of pineal extract on the PTG. However, there is no study on the effects of melatonin on the ultrastructure of the PTG. We investigated ultrastructural changes in the PTG of golden hamsters after administration of melatonin.Materials and methods. Three-month-old female golden hamsters with an average body weight of 130 g were divided into 5 groups of 5 animals each. One group was given 0.2.ml of distilled water as controls. The remaining groups were given 0.2 ml of melatonin solution at a dose of 0.8 mg/100 g body weight. The PTG of each group was removed under pentobarbital anesthesia at 1, 5, 24 and 48 hours after injection. The PTG was immersed in a mixture of 2.5% glutaraldehyde and 2% OsO4, dehydrated through acetone and embedded in Epon 812.

An Electron Microscopic Study of the Human Gastric Epithelia with Special Reference to the Cardinal Morphological Changes of the Chief Cells Induced by Insulin Stimulus

1972 ◽  
Vol 30 ◽  
pp. 344-345
Author(s):  
Hiroshi Saito ◽  
Goro Asano ◽  
Kaoru Aihara ◽  
Katsunari Fukushi ◽  
Minoru Yoshida ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Morphological Changes ◽  
Regular Insulin ◽  
Endoscopic Biopsy ◽  
Granular Endoplasmic Reticulum ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Chief Cells ◽  
Microscopic Studies ◽  
The One

This short communication is dealt with the ultrastructural changes of the chief cells in insulin stimulus in chronic gastritic condition. The bio gastro-endoscopic biopsy was obtained and pepsin activity of the gastric juice was measured in respective cases. Regular insulin of 0.15U/kg was administrated intra-muscularly and in pre-administration of insulin, 10 minutes, 20 minutes and 30 minutes after administration, biopsied specimens were subjected for electron microscopic studies.In the pre-treated chief cells, extensive development of the cysternal structures of the granular endoplasmic reticulum in basal aspect of the cytoplasm and spherical or oval shaped, light homogeneous zymogen granules in supranuclear region and especially apical aspect of the cytoplasm were featured. Moreover, other type of the chief cells as the one characterized by their fragmented and saccular dilated granular endoplasmic reticulum in basal aspect of the cytoplasm, also exist.

Scanning Electron Microscopic Study of Flowing Erythrocytes in Hepatic Sinusoids as Revealed by “in Vivo Cryotechnique”

1997 ◽  
Vol 3 (S2) ◽  
pp. 239-240
Author(s):  
N. Terada ◽  
Y. Fujii ◽  
Y. Kato ◽  
H. Ueda ◽  
T. Baba ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Vessels ◽  
Morphological Changes ◽  
Surface Membrane ◽  
Heart Beat ◽  
Ultrastructural Changes ◽  
Sodium Pentobarbital ◽  
Electron Microscopic ◽  
In Vivo Cryotechnique

The flowing behavior of individual erythrocytes in blood vessels is usually determined by their deformability, which is controlled mainly by the nature of their interior constituents and the flexibility of their surface membrane. Moreover, the physical behavior of erythrocytes passing through capillaries has been examined in vivo by light microscopy. However, little has been known about ultrastructural changes of such erythrocyte shapes flowing in blood vessels in vivo. Recently, a new technique was developed for freezing cells and tissues in vivo without stopping the blood supply, which was referred to as “in vivo cryotechnique”.This method has been also suitable for obtaining informations about dynamic morphological changes.Seven female Balb/c mice were anesthetized peritoneally with sodium pentobarbital (100μg/g body weight), and their abdomen was opened through a pararectus incision. For artificial cardiac arrest, some mice were anesthetized with an excessive dose of the anesthetic (500μg/g body weight), their respiration and heart-beat were completely stopped, and the following procedures were done within one minute. A liver was put on a plastic plate without disturbance of blood circulation, and the “in vivo cryotechnique” was performed. Briefly, a cryoknife was pushed into the liver as fast as possible and the tissue was immediately poured with liquid isopentane-propane mixture (-193°C) (Fig.la,b).

Effects of Combination Chemical Peel on Facial Photodamaged Skin

1998 ◽  
Vol 15 (3) ◽  
pp. 263-271 ◽  
Author(s):  
May H. El Samahy ◽  
Mohamed M. Ghoz ◽  
Naglaa Ramzy
Keyword(s):  
Glycolic Acid ◽  
Electron Microscopic Examination ◽  
Ultrastructural Changes ◽  
Elastic Fibers ◽  
Electron Microscopic ◽  
Trichloracetic Acid ◽  
Age Related ◽  
Photodamaged Skin ◽  
Microscopic Studies ◽  
Activated Fibroblasts

Introduction: Chemical peeling involves the topical application of a wounding agent with the goal of effecting an organized regeneration of the skin. The histologic and ultrastructural features of actinic and age-related damage include structural abnormalities that disrupt normal epidermal and dermal architecture. The purpose of the present study is to evaluate the clinical and histologic effects of an enhanced medium-depth peel on photodamaged skin. We aimed to correlate the clinical and histologic findings with the ultrastructural changes occurring after the peel. These ultrastructural features are supposed to be more precise and informative than the clinical or histological response. They may also be employed as markers of peel response. Materials and Methods: In the present study, five patients with actinically damaged skin underwent enhanced medium-depth peels using 70% glycolic acid and 35% trichloracetic acid. Biopsy specimens were taken before the peel and 3 months after the peel for histologic and electron microscopic examination. Results: Clinical resolution of actinic damage corresponded with restoration of epidermal polarity. Characteristic histologic and ultrastructural features of the skin after peeling include markedly decreased epidermal intracytoplasmic vacuoles, decreased elastic fibers, increased activated fibroblasts, and organized parallel arrays of collagen fibrils. The diameters of individual fibrils are consistent with recent production of collagen by activated fibroblasts. Conclusion: Glycolic acid—tricholoro-acetic acid (GA-TCA) is an effective combination for a medium-depth peel in photodamaged skin both clinically and histologically. Electron microscopic studies following medium-depth peels reveal changes more profound than those seen histologically. The characteristic changes occurring in the keratinocytes, collagen, and elastic fibrils may be considered as guidelines or markers of the peel response.

Electron-Microscopic Study of the Parathyroid Gland of Epinephrine-Treated Golden Hamsters Subjected to Hypergravity Environment

1991 ◽  
Vol 142 (1) ◽  
pp. 81-86 ◽  
Author(s):  
S. Shoumura ◽  
S. Emura ◽  
M. Utsumi ◽  
H. Chen ◽  
T. Yamahira ◽  
...  
Keyword(s):  
Parathyroid Gland ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Golden Hamsters

Electron Microscopic Studies of Fillers in Rubber. III. Effect of Milling on the Dispersion of Fillers

1956 ◽  
Vol 29 (3) ◽  
pp. 1003-1010 ◽  
Author(s):  
Eiji Suito ◽  
Masafumi Arakawa ◽  
Hiroshi Hasegawa ◽  
Yonemasa Furusawa
Keyword(s):  
Electron Microscope ◽  
Marked Effect ◽  
The State ◽  
Replica Method ◽  
Interesting Problem ◽  
Electron Microscopic ◽  
Vulcanized Rubber ◽  
Microscopic Studies ◽  
Filler Particles ◽  
The Relationship

Abstract In the study of types of fillers which have a marked effect on the properties of rubber, information as to how the filler particles are dispersed in rubber is a prerequisite. The authors have already reported on the state of dispersion of various fillers in vulcanized rubber, observed under an electron microscope by the replica method. How the dispersion of these fillers affects the properties of rubber is an interesting problem. Since, in the earlier work, filler particles were observed to orient themselves in certain directions, in this report the relationship between the state of dispersion observed under an electron microscope of filler particles in rubber milled in different ways and the resulting characteristics of the mixtures were examined.

scholarly journals STUDIES ON THE MECHANISM OF EXPERIMENTAL PROTEINURIA INDUCED BY RENIN

1964 ◽  
Vol 120 (4) ◽  
pp. 677-690 ◽  
Author(s):  
Sharad D. Deodhar ◽  
Francis E. Cuppage ◽  
E. Gableman
Keyword(s):  
Enzymatic Activity ◽  
Adrenal Glands ◽  
Electron Microscopic ◽  
Chemically Modified ◽  
Glomerular Capillary ◽  
Enzymatic Action ◽  
Microscopic Studies ◽  
Inactive Renin ◽  
The Relationship ◽  
Permissive Role

Renin-induced proteinuria in the rat was investigated, with special emphasis on the relationship between the enzymatic activity and the proteinuric effect of renin. The dependence of the proteinuric effect on the enzymatic activity was shown by using (a) renin preparations of widely varying purity and (b) chemically modified "active" and "inactive" renin derivatives. Angiotensin II, the pressor product of the enzymatic action of renin, also produced significant proteinuria. Adrenalectomy abolished the proteinuria induced by renin. Proteinuria, however, occurred as a result of pretreatment with DOCA, or aldosterone, or without treatment, 7 to 8 weeks after adrenalectomy. Electron microscopic studies of the kidney at the time of maximal proteinuria showed focal flattening and fusion of epithelial foot processes, as well as swelling and vesicle formation in endothelial and epithelial cells of the glomeruli. Studies with intravenously injected saccharated iron oxide showed increased permeability of the glomerular capillary basement membrane to these particles. These changes were transient and were not seen 24 hours after renin injection. Adrenalectomy prevented these changes. It is concluded that renin, acting through angiotensin, causes glomerular capillary damage with increased permeability of these structures to protein and resultant proteinuria. The adrenal glands participate in a permissive role in this phenomenon.

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