scholarly journals Detection ofLegionella pneumophilain environmental water samples using a fluorescein conjugated monoclonal antibody

1989 ◽  
Vol 103 (1) ◽  
pp. 105-112 ◽  
Author(s):  
T. Makin ◽  
C. A. Hart
Keyword(s):  
Monoclonal Antibody ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Indirect Fluorescent Antibody Test ◽  
Specific Binding ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Culture Positive

SUMMARYSixty-three environmental water samples from various sources were examined for the presence ofLegionella pneumophilawith a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detectedL. pneumophilain 94% and 100% of environmental water samples which were culture and IFAT positive forL. pneumophila, respectively. IFAT detected 69% ofL. pneumophilaculture positive samples. Cultures ofL. pneumophilaserogroups 1 to 12, 14 and nonL. pneumophilabacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups ofL. pneumophilaand did not cross react with any non-L. pneumophilabacteria.L. pneumophilain environmental samples was easy to detect against a clear dark background when stained with GS.

A monoclonal antibody toBabesia divergenswhich inhibits merozoite invasion

Parasitology ◽  
1987 ◽  
Vol 94 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Caroline M. Winger ◽  
Elizabeth U. Canning ◽  
J. D. Culverhouse
Keyword(s):  
Monoclonal Antibody ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Polyacrylamide Gel Electrophoresis ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Merozoite Invasion ◽  
Babesia Divergens ◽  
Sds Page ◽  
Bovine Erythrocytes

SUMMARYA monoclonal antibody-producing hybridoma cell line has been raised against merozoites ofBabesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.

scholarly journals Serological cross-reaction betweenLegionella pneumophilaand Campylobacter in the indirect fluorescent antibody test

1992 ◽  
Vol 109 (2) ◽  
pp. 291-295 ◽  
Author(s):  
T. C. J. Boswell ◽  
G. Kudesia
Keyword(s):  
Indirect Immunofluorescence ◽  
Legionella Pneumophila ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Cross Reaction ◽  
Indirect Fluorescent Antibody ◽  
Igm Antibodies ◽  
Specific Igg

SUMMARYSera from 50 patients with culture-proven Campylobacter gastroenteritis were examined for the presence of antibodies toLegionella pneumophila. Ten patients (20%) had a positive titre (≥16) as measured by indirect immunofluorescence. Antibodies were detected in only 1 of 36 acute sera but in 10 of 14 (71%) sera obtained more than 10 days after the onset of symptoms. All positive sera contained specific IgM antibodies but specific IgG or IgA could not be detected in any sample. No legionella antibodies could be detected in sera from 42 similar patients with salmonella gastroenteritis. These results were shown to be due to serological cross-reaction betweenL. pneumophilaand Campylobacter.

A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4′-(aminomethyl)fluorescein

2015 ◽  
Vol 7 (10) ◽  
pp. 4246-4251 ◽  
Author(s):  
Pinong Huang ◽  
Suqing Zhao ◽  
Sergei A. Eremin ◽  
Shengwu Zheng ◽  
Dan Lai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Bisphenol A ◽  
Water Samples ◽  
Fluorescence Polarization ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Fluorescence Polarization Immunoassay

A homogeneous fluorescence polarization immunoassay (FPIA) was developed to detect bisphenol A in environmental water samples.

Improving Mycobacterium spp. detection in water: a new methodological proposal

2004 ◽  
Vol 4 (2) ◽  
pp. 103-106
Author(s):  
R. Santos ◽  
S. Gonçalves ◽  
F. Macieira ◽  
F. Oliveira ◽  
R. Rodrigues ◽  
...  
Keyword(s):  
Water Samples ◽  
Recovery Rate ◽  
Opportunistic Infections ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Sample Treatment ◽  
Detection Time ◽  
Fast Detection ◽  
Enrichment Step ◽  
Mycobacterium Spp

In recent years, non-tuberculous mycobacteria (NTM), once considered merely environmental saprophytes, have emerged as a major cause of opportunistic infections. There is no evidence of human-to-human transmission but they have been found in several environmental water samples. It is, therefore, of the utmost importance to develop methods of rapidly and accurately detecting non-tuberculous mycobacteria in water samples. To obtain a maximum recovery rate and a reduction of Mycobacterium spp. detection time in water samples, different decontamination, enrichment procedures and antibiotics supplements were tested before the inoculation into the Bactec® system. The proposed method of sample treatment (decrease in the decontamination time, followed for a peptone pre-enrichment step and an aztreonam and cefepime supplement) before the inoculation into the Bactec® system proved to be a good option for reliable and fast detection of Mycobacterium spp. in water samples.

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