scholarly journals Human herpesvirus-8 antibodies and DNA in HIV-1 infected patients in South Africa

2003 ◽  
Vol 131 (3) ◽  
pp. 1125-1129 ◽  
Author(s):  
L. ALAGIOZOGLOU ◽  
L. MORRIS ◽  
H. BREDELL ◽  
D. J. MARTIN ◽  
F. SITAS
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Outpatient Clinics ◽  
Human Herpesvirus 8 ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Herpesvirus 8 ◽  
Increased Risk ◽  
Hiv 1

HIV-infected individuals with high levels of IgG antibodies against human herpesvirus-8 (HHV-8) are at increased risk of developing Kaposi's sarcoma. The aim of this study was to measure the association between HHV-8 viraemia and IgG antibody responses (by immunofluorescence) in a group of 201 HIV-infected individuals attending outpatient clinics, 91 in-patients with AIDS and 87 HIV-infected patients admitted with Kaposi's sarcoma. Compared to HIV-infected outpatients, the adjusted odds ratio in relation to Kaposi's sarcoma was 15·4 (95% CI 4·4–54·2) in those with viraemia, 25·1 (95% CI 6·6–95·6) in those with a positive immunofluorescent signal and ∞ (lower exact CI 33·6) in those with a high immunofluorescent signal (all P trend <0·001). Among those without HHV-8 viraemia, 23% were IgG-positive, but only 5·5% had a high immunofluorescent signal. In those who were viraemic, 89·1% were IgG-positive, and 28·2% had a high immunofluorescent signal, suggesting viraemia is associated with high HHV-8 immunofluorescence IgG signal.

Detection and Titration of Human Herpesvirus-8–Specific Antibodies in Sera From Blood Donors, Acquired Immunodeficiency Syndrome Patients, and Kaposi's Sarcoma Patients Using a Whole Virus Enzyme-Linked Immunosorbent Assay

Blood ◽  
1998 ◽  
Vol 92 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Louise G. Chatlynne ◽  
William Lapps ◽  
Michael Handy ◽  
Yao Q. Huang ◽  
Rizwan Masood ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Kaposi's Sarcoma ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Antibody Detection ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Herpesvirus 8

A human herpesvirus-8 (HHV-8) enzyme-linked immunosorbent assay (ELISA) with a whole virus lysate as antigen was developed and used to measure the seroprevalence rate and levels of IgG antibodies to HHV-8 in sera/plasma of various patient groups and blood donors. The virus antigen was prepared from the KS-1 cell line, which produces lytic virus, and therefore contains a broad array of viral proteins. Seroprevalence studies using this ELISA showed the following: 10 of 91 blood donors (11%) had an average HHV-8 antibody titer of 118; 67 of 72 (93%) classic Kaposi's sarcoma (KS) patients were positive with an average titer of 14,111; and 57 of 62 (92%) KS/human immunodeficiency virus (HIV) patients were positive with an average titer of 4,000. A study on a very limited number of serial serum samples from patients before and after diagnosis with KS showed highly elevated antibody titers to HHV-8 virus after KS lesions developed. Preliminary data show that 50% of the sera from HIV-1+ homosexual patients contain IgG antibodies to HHV-8 suggesting that this population is at high risk for developing KS. Antibody results correlated well with the confirmatory immunofluorescent assays (IFA) using KS-1 cells as the substrate. This HHV-8 IgG antibody detection ELISA is sensitive and specific and does not cross-react with Epstein-Barr virus (EBV) or other human herpesviruses. The results of this HHV-8 antibody survey suggest that this rapid ELISA assay can be used to screen large numbers of sera to find those at risk for developing KS.

scholarly journals Identification of Antigenic Proteins Encoded by Human Herpesvirus 8 and Seroprevalence in the General Population and among Patients with and without Kaposi's Sarcoma

2000 ◽  
Vol 74 (8) ◽  
pp. 3478-3485 ◽  
Author(s):  
Harutaka Katano ◽  
Takuya Iwasaki ◽  
Nobuyoshi Baba ◽  
Masanori Terai ◽  
Shigeo Mori ◽  
...  
Keyword(s):  
General Population ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Herpesvirus 8 ◽  
Antigen Elisa ◽  
Antigenic Proteins

ABSTRACT To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded proteins were produced as glutathioneS-transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four proteins encoded by open reading frames (ORFs) K8.1, 59, 65, and 73 in a Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these four proteins as antigens (mixed-antigen ELISA) revealed that all 26 sera derived from KS patients (24 with and 2 without human immunodeficiency virus infection) became positive for anti-HHV8 antibodies. The presence of HHV8 was demonstrated in 14 (1.4%) of 1,004 sera from the Japanese general population and 10 (1.9%) of 527 sera from patients without HHV8-associated diseases. The presence of immunoglobulin G (IgG) and IgM antibodies against HHV8 examined further by the mixed-antigen ELISA and Western blotting revealed IgG antibody in all ELISA-positive sera, while IgM antibody against ORF K8.1 was absent. These data suggest that the ORF 73 and 65 proteins are potent antigens for a sensitive serological assay.

Kaposi's sarcoma and human herpesvirus 8 infection do not protect HIV-1 infected homosexual men from AIDS dementia complex

AIDS ◽  
2001 ◽  
Vol 15 (16) ◽  
pp. 2165-2169 ◽  
Author(s):  
Neil Renwick ◽  
Gerrit Jan Weverling ◽  
Teysir Halaby ◽  
Peter Portegies ◽  
Margreet Bakker ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Aids Dementia Complex ◽  
Human Herpesvirus 8 ◽  
Homosexual Men ◽  
Herpesvirus 8 ◽  
Aids Dementia ◽  
Hiv 1

TIMING OF ANTIBODIES TO HUMAN HERPESVIRUS-8 (HHV-8) AMONG HIV-1 SEROCONVERTERS AND PROGRESSION TO KAPOSI'S SARCOMA

1999 ◽  
Vol 21 (1) ◽  
pp. A18 ◽  
Author(s):  
Lisa P. Jacobson ◽  
Gayle Springer ◽  
Frank Jenkins ◽  
Haroutune Armenian ◽  
Alvaro Muñoz
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Hiv 1

Absence of an association between the presence of human herpesvirus 8 antibodies and the development of Kaposi's sarcoma in HIV-1-infected patients receiving antiretroviral therapy

AIDS ◽  
2000 ◽  
Vol 14 (10) ◽  
pp. 1455-1457 ◽  
Author(s):  
Adele Caterino-de-Araujo ◽  
Paulo H. L. Carbone ◽  
Fábio L. B. Martinelli ◽  
Elizabeth de los Santos-Fortuna ◽  
Abdiel A. Moreira ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Hiv 1

scholarly journals Human Herpesvirus 8 Induces Polyfunctional B Lymphocytes That Drive Kaposi’s Sarcoma

mBio ◽  
2014 ◽  
Vol 5 (5) ◽  
Author(s):  
Emilee R. Knowlton ◽  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Lauren M. Lepone ◽  
Sagar V. Nadgir ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Inhibitory Protein ◽  
Herpesvirus 8 ◽  
Cytokines And Chemokines ◽  
Hiv 1

ABSTRACTKaposi’s sarcoma (KS) is an unusual neoplasia wherein the tumor consists primarily of endothelial cells infected with human herpesvirus 8 (HHV-8; Kaposi’s sarcoma-associated herpesvirus) that are not fully transformed but are instead driven to excess proliferation by inflammatory and angiogenic factors. This oncogenic process has been postulated but unproven to depend on a paracrine effect of an abnormal excess of host cytokines and chemokines produced by HHV-8-infected B lymphocytes. Using newly developed measures for intracellular detection of lytic cycle proteins and expression of cytokines and chemokines, we show that HHV-8 targets a range of naive B cell, IgM memory B cell, and plasma cell-like populations for infection and induction of interleukin-6, tumor necrosis factor alpha, macrophage inhibitory protein 1α, macrophage inhibitory protein 1β, and interleukin-8in vitroand in the blood of HHV-8/HIV-1-coinfected subjects with KS. These B cell lineage subsets that support HHV-8 infection are highly polyfunctional, producing combinations of 2 to 5 of these cytokines and chemokines, with greater numbers in the blood of subjects with KS than in those without KS. Our study provides a new paradigm of B cell polyfunctionality and supports a key role for B cell-derived cytokines and chemokines produced during HHV-8 infection in the development of KS.IMPORTANCEKaposi’s sarcoma (KS) is the most common cancer in HIV-1-infected persons and is caused by one of only 7 human cancer viruses, i.e., human herpesvirus 8 (HHV-8). It is unclear how this virus causes neoplastic transformation. Development and outgrowth of endothelial cell lesions characteristic of KS are hypothesized to be dependent on virus replication and multiple immune mediators produced by the KS cells and inflammatory cells, yet the roles of these viral and cell factors have not been defined. The present study advances our understanding of KS in that it supports a central role for HHV-8 infection of B cells inducing multiple cytokines and chemokines that can drive development of the cancer. Notably, HIV-1-infected individuals who developed KS had greater numbers of such HHV-8-infected, polyfunctional B cells across a range of B cell phenotypic lineages than did HHV-8-infected persons without KS. This intriguing production of polyfunctional immune mediators by B cells serves as a new paradigm for B cell function and classification.

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