Vibrio cholerae O1 El Tor from southern Vietnam in 2010 was molecularly distinct from that present from 1999 to 2004

2015 ◽  
Vol 144 (6) ◽  
pp. 1241-1247 ◽  
Author(s):  
V. H. NGUYEN ◽  
H. T. PHAM ◽  
T. T. DIEP ◽  
C. D. H. PHAN ◽  
T. Q. NGUYEN ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Environmental Isolates ◽  
Southern Vietnam ◽  
Large Outbreak ◽  
Vibrio Cholerae O1 ◽  
El Tor

SUMMARYThe Vibrio cholerae O1 (VCO1) El Tor biotype appeared during the seventh cholera pandemic starting in 1961, and new variants of this biotype have been identified since the early 1990s. This pandemic has affected Vietnam, and a large outbreak was reported in southern Vietnam in 2010. Pulsed-field gel electrophoresis (PFGE) and multilocus variable-number tandem-repeat analyses (MLVA) were used to screen 34 VCO1 isolates from the southern Vietnam 2010 outbreak (23 patients, five contact persons, and six environmental isolates) to determine if it was genetically distinct from 18 isolates from outbreaks in southern Vietnam from 1999 to 2004, and two isolates from northern Vietnam (2008). Twenty-seven MLVA types and seven PFGE patterns were identified. Both analyses showed that the 2008 and 2010 isolates were distinctly clustered and separated from the 1999–2004 isolates.

scholarly journals Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

2000 ◽  
Vol 38 (11) ◽  
pp. 4249-4253 ◽  
Author(s):  
Pallavi Garg ◽  
Ranjan K. Nandy ◽  
Papiya Chaudhury ◽  
Nandini Roy Chowdhury ◽  
Keya De ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Vibrio Cholerae ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Pulsed Field Gel Electrophoresis ◽  
Vibrio Cholerae O1 ◽  
El Tor ◽  
Restriction Fragment Length

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular characterization by ribotyping, restriction fragment length polymorphism, and pulsed-field gel electrophoresis indicated that recent Inaba strains are remarkably different from the earlier Inaba strains but are very similar to the prevailing V. choleraeO1 Ogawa El Tor biotype strains. The antibiograms of the Inaba strains were also similar to those of the recent V. cholerae Ogawa strains. These V. cholerae O1 Inaba strains appear to have evolved from the currently prevailing Ogawa strains and are likely to dominate in the coming years.

scholarly journals Pulsed-Field Gel Electrophoresis-Based Molecular Comparison of Vibrio cholerae O1 Isolates from Domestic and Imported Cases of Cholera in Japan

2000 ◽  
Vol 38 (1) ◽  
pp. 424-426
Author(s):  
Eiji Arakawa ◽  
Toshiyuki Murase ◽  
Shigeru Matsushita ◽  
Toshio Shimada ◽  
Shiro Yamai ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Imported Cases ◽  
Molecular Comparison ◽  
Pulse Type ◽  
Vibrio Cholerae O1 ◽  
El Tor

ABSTRACT Sixty-seven Vibrio cholerae O1 El Tor isolates (36 domestic and 31 imported) were classified into 19 subtypes by Not I- and Sfi I-digested pulsed-field gel electrophoresis. Twenty-five of 36 domestic and 4 imported isolates were assigned to a Not I-A1– Sfi I-A1 subtype, suggesting that this pulse type is widely distributed in Asia and Japan.

scholarly journals Vibrio cholerae El Tor strains linked to global cholera are homogeneous by pulsed-field gel electrophoresis

2021 ◽  
Author(s):  
Fatema-Tuz Johura ◽  
Sahitya Ranjan Biswas ◽  
Shah M Rashed ◽  
Mohammad Tarequl Islam ◽  
Saiful Islam ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Homogeneous Cluster ◽  
Vibrio Cholerae O1 ◽  
Spatio Temporal ◽  
Pandemic Strains ◽  
El Tor

Vibrio cholerae O1 El Tor, causative agent of the ongoing seventh cholera pandemic, is native to the aquatic environment of the Ganges Delta, Bay of Bengal (GDBB). Recent studies traced pandemic strains to the GDBB and proposed global spread of cholera had occurred via intercontinental transmission. In the research presented here, Not I-digested genomic DNA extracted from V. cholerae O1 clinical and environmental strains isolated in Bangladesh during 2004 – 2014 was analyzed by pulsed-field gel electrophoresis (PFGE). Results of cluster analysis showed 94.67% of the V. cholerae isolates belonged to clade A and included the majority of clinical isolates of spatio-temporal origin and representing different cholera endemic foci. The rest of the strains were estuarine, all environmental isolates from Mathbaria, Bangladesh, and occurred as singletons, clustered in clades B and C, or in the small clades D and E. Cluster analysis of the Bangladeshi strains and including 157 El Tor strains from thirteen countries in Asia, Africa, and the Americas revealed 85% of the total set of isolates belonged to clade A, indicating all were related, yet did not form an homogeneous cluster. Overall, 15% of the global strains comprised multiple small clades or segregated as singletons. Three sub-clades could be discerned within the major clade A, reflecting distinct lineages of V. cholerae El Tor associated with cholera in Asia, Africa, and the Americas. The presence in Asia and the Americas of non-pandemic V. cholerae El Tor populations differing by PFGE and from strains associated with cholera globally suggests different ecotypes are resident in distant geographies.

scholarly journals Comparison of Multilocus Variable-Number Tandem-Repeat Analysis with Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis for Enterococcus faecalis

2011 ◽  
Vol 60 (4) ◽  
pp. 335-339 ◽  
Author(s):  
EWA SADOWY ◽  
ALEKSANDRA SIEŃKO ◽  
WALERIA HRYNIEWICZ
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Tandem Repeats ◽  
Diversity Index ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Discriminatory Power ◽  
Pulsed Field ◽  
High Discriminatory Power

Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus sequence types and 67 pulsed-field gel electrophoresis (PFGE) profiles. MLVA showed high discriminatory power, discerning 111 different types (diversity index equal 98.9%). The concordance MLVA/MLST and MLVA/PFGE was 0.95 and 0.74, respectively. High discriminatory power of MLVA indicates its utility for local epidemiology such as outbreak investigation, and for differentiation of clones defined by other methods.

scholarly journals Molecular epidemiology of Vibrio cholerae O1 in northern Vietnam (2007–2009), using multilocus variable-number tandem repeat analysis

2016 ◽  
Vol 65 (9) ◽  
pp. 1007-1012 ◽  
Author(s):  
Dong Tu Nguyen ◽  
Tuan Cuong Ngo ◽  
Thanh Huong Le ◽  
Hoai Thu Nguyen ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Vibrio Cholerae ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Northern Vietnam ◽  
Repeat Analysis ◽  
Vibrio Cholerae O1
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