Feeding stem–leaf–pod explants of pea (Pisum sativum L.) with d-chiro-inositol or d-pinitol modifies composition of α-d-galactosides in developing seeds

2010 ◽  
Vol 20 (4) ◽  
pp. 213-221 ◽  
Author(s):  
Lesław B. Lahuta ◽  
Wojciech Święcicki ◽  
Tomasz Dzik ◽  
Ryszard J. Górecki ◽  
Marcin Horbowicz
Keyword(s):  
Pisum Sativum ◽  
Soluble Carbohydrates ◽  
Raffinose Family Oligosaccharides ◽  
Developing Seeds ◽  
Pisum Sativum L ◽  
Pea Seeds ◽  
Low Levels ◽  
Stem Leaf

AbstractFeeding stem–leaf–pod explants with d-chiro-inositol and d-pinitol was used as a method to modify α-d-galactosides in developing pea (Pisum sativum) seeds. Four genotypes differing in the composition of raffinose, stachyose and verbascose (raffinose family oligosaccharides or RFOs) in seeds – high RFOs (cv. Tiny), low RFOs (SZD175) and low verbascose (cv. Hubal and cv. Wt 506) – were studied. Although seeds of all examined pea lines were able to take up both d-chiro-inositol and d-pinitol, only d-chiro-inositol was effectively converted into its galactosides: mainly fagopyritol B1 (O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol) and fagopyritol B2 (O-α-d-galactopyranosyl-(1 → 6)-O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol). In seeds of pea lines naturally containing low levels of verbascose (cv. Hubal) and low RFOs (SZD175), the enhanced accumulation of fagopyritols depressed the RFO level by c. 64 and 20%, respectively. Moreover, in both genotypes, about 25 and 30% of total galactose bound in α-d-galactosides occurred in fagopyritols. d-Pinitol present in the pea seeds was converted into monogalactosides, but their accumulation was several-fold lower than that of fagopyritols and did not significantly influence the accumulation of RFOs. Pea seeds with the composition of soluble carbohydrates modified by feeding with either of the cyclitols were able to complete germination.

Proximate and mineral composition of field peas

1997 ◽  
Vol 77 (1) ◽  
pp. 101-103 ◽  
Author(s):  
T. D. Warkentin ◽  
A. G. Sloan ◽  
S. T. Ali-Khan
Keyword(s):  
Pisum Sativum ◽  
Key Words ◽  
Mineral Composition ◽  
Seed Yield ◽  
Total Protein ◽  
Field Pea ◽  
Pisum Sativum L ◽  
Field Peas ◽  
Pea Seeds ◽  
Proximate And Mineral Composition

Field pea seeds from 10 cultivars grown at two locations in Manitoba in 1986 and 1987 were analyzed for proximate and mineral profiles. Cultivars differed significantly in their level of total protein, crude fat, ADF, and all minerals tested. However, differences were not extremely large and were comparable to European reports. Location-year also had a significant effect on the levels of total protein, ADF, and all minerals tested. In most cases, the warmest location-year produced relatively higher levels of minerals, ash, and total protein, and lower seed yield than the coolest location-year. Key words: Field pea, Pisum sativum L., mineral

scholarly journals Reduction in the content of antinutritional substances in pea seeds (Pisum sativum L.) by different treatments

2011 ◽  
Vol 50 (No. 11) ◽  
pp. 519-527 ◽  
Author(s):  
R. Dvořák ◽  
A. Pechová ◽  
L. Pavlata ◽  
J. Filípek ◽  
J. Dostálová ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Seed Treatment ◽  
Production Traits ◽  
Pisum Sativum L ◽  
Heat Treated ◽  
Pea Seeds ◽  
Reactor Temperature ◽  
Pre Treatment ◽  
Pea Seed ◽  
Representative Samples

The goal of the trial was to reduce the content of antinutritional substances in pea (Pisum sativum L.) seeds in order to enhance its use in livestock nutrition. A variety of field pea (Pisum sativum L.) with a high content of antinutritional substances and favourable production traits (Gotik) was chosen. Native and heat-treated pea seeds were used to collect representative samples (n = 6) for analytical purposes. The technology (V-0 technology, Czech patent No. 285745) was further modified by adjusting the reactor temperature, the duration of exposure to that temperature, and the duration of ageing of the material treated in this way (V-I and V-II technologies). The methodology of treatment is based on exposing pea seeds to vapour, organic acids and selected oxides.The monitored parameters included antinutritional substances. As far as the antinutritional substances were concerned, the content of trypsin inhibitors in native pea seeds (P) was around 15.4 ± 0.5 TIU. After treatment with technologies V-0, V-I, and V-II its activity dropped by 83.8, 80.5 and 83.8%, respectively. The pre-treatment titre of lectins (P) was 717 ± 376. It dropped by 70.3, 35.7 and 73.2% after treatment with technologies V-0, V-I and V-II, respectively. The content of tannins measured by the amount of gallic acid in native pea seeds was 49.1 ± 2.7 mg per kg. It dropped by 41.4, 32.0 and 46.2% after the application of the above-mentioned technologies. The content of indigestible oligosaccharides causing flatulence was less affected by the treatments. The pre-treatment content of raffinose was 9.5 ± 0.5 g/kg. The drop associated with the treatment was 9.5, 6.3 and 10.5%, respectively. The pre-treatment content of stachyose was 21.4 ± 0.8 g/kg and after treatment with technologies V-0 and V-II it dropped by 7.0% and by 16.4%, respectively. The application of technology V-I did not result in a drop in the content of stachyose. The content of verbascose in native pea seeds was 16.1 g/kgand the treatment with technologies V-0; V-I and V-II resulted in a drop by 7.5, 5.6 and 20.5%, respectively. As for the detected phenolic acids, with the exception of caffeic acid, not a drop, but an increase in their content was recorded. Isoflavone oestrogens such as daidzein and genistein also recorded a small increase in their content. The results of the trial lead us to conclude that the above-described methods of pea seed treatment, especially the V-II variant, proved to be useful and can be recommended for practical use.  

scholarly journals The Fate of l-Phenylalanine Fed to Germinating Pea Seeds, Pisum sativum (L.) var. Alaska, during Imbibition

1971 ◽  
Vol 47 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Constance Nozzolillo ◽  
K. B. Paul ◽  
C. Godin
Keyword(s):  
Pisum Sativum ◽  
Pisum Sativum L ◽  
Pea Seeds

Release of Amino Acids from Cotyledons of Developing Seeds of Pea (Pisum sativum L.)

1989 ◽  
Vol 134 (5) ◽  
pp. 592-597 ◽  
Author(s):  
F.C. Lanfermeijer ◽  
J.W. Koerselman-Kooij ◽  
C. Kollöffel ◽  
A.C. Borstlap
Keyword(s):  
Amino Acids ◽  
Pisum Sativum ◽  
Developing Seeds ◽  
Pisum Sativum L

scholarly journals Accumulation of Phosphorus-Containing Compounds in Developing Seeds of Low-Phytate Pea (Pisum sativum L.) Mutants

Plants ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 1-26 ◽  
Author(s):  
Arun Shunmugam ◽  
Cheryl Bock ◽  
Gene Arganosa ◽  
Fawzy Georges ◽  
Gordon Gray ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Developing Seeds ◽  
Pisum Sativum L ◽  
Phosphorus Containing

Leucyl β -Naphthylamidase Activities in Developing Seeds and Seedlings of Pisum sativum L

1977 ◽  
Vol 4 (4) ◽  
pp. 571 ◽  
Author(s):  
MD Collier ◽  
DR Murray
Keyword(s):  
Pisum Sativum ◽  
Maximum Rate ◽  
Storage Proteins ◽  
Total Activity ◽  
Selective Inhibitor ◽  
Cellular Proteins ◽  
Developing Seeds ◽  
Pisum Sativum L ◽  
Soluble Enzymes ◽  
Germinating Seeds

The activities of two soluble enzymes which hydrolyse L-leucyl �-naphthylamide have been measured in extracts from tissues of seeds and seedlings of Pisum sativum L. by using the chelator 1,10-phenanthroline as a selective inhibitor. In all the tissues studied, the phenanthroline-insensitive enzyme contributed the major proportion of the total activity against this substrate. In developing seeds, most of the activity of both enzymes is found in the maturing cotyledons, which develop maximum phenanthroline-sensitive and -insensitive activities respectively of 0.51 and 1.26 �mol per min per cotyledon (cv. Melbourne Market, dwarf) or 0.84 and 1.32 �mol per min per cotyledon (cv. Telephone, tall). In the cotyledons of germinating seeds, both enzyme activities increase within 24 h to values which are substantially lower than the maximum values found during development. These activities are maintained between 1 and 6 days from imbibition then they decline rapidly during the period of maximum rate of removal of protein from the cotyledon. The highest activities of both enzymes occur in tissues which are very active metabolically. This supports the view that they function as aminopeptidases in the general turnover of cellular proteins, rather than playing some additional specific role in the mobilization of storage proteins during germination.

Sign in / Sign up

Export Citation Format

Share Document