scholarly journals THE HEDYOTIS–OLDENLANDIA–KOHAUTIA COMPLEX (RUBIACEAE) IN NEPAL: A STUDY OF FRUIT, SEED AND POLLEN CHARACTERS AND THEIR TAXONOMIC SIGNIFICANCE

2009 ◽  
Vol 66 (3) ◽  
pp. 371-390 ◽  
Author(s):  
S. Neupane ◽  
S. Dessein ◽  
T. J. Motley
Keyword(s):  
Pollen Grains ◽  
Seed Morphology ◽  
Marked Variation ◽  
Taxonomic Significance ◽  
Pollen Characters ◽  
Electron And Light Microscopy ◽  
Seed Characters ◽  
Fruit Dehiscence ◽  
Natural Groups ◽  
Scanning Electron

Pollen and seed characters of 12 Nepalese representatives of the Hedyotis–Oldenlandia–Kohautia complex (Spermacoceae s.l.; Rubiaceae) were investigated morphologically using scanning electron and light microscopy. The members of the complex were found to show remarkable variation in fruit, seed and pollen features. Pollen grains were all colporate with the aperture number varying from 3–4 to occasionally 5. The ectoaperture was a colpus, and the endoaperture was an endocingulum, a lalongate endocolpus or an endocolpus combined with an annulus around the mesoporus. Sexine ornamentation was variable, being perforate, reticulate or microreticulate. Three species were found to have a double reticulum. Supratectal elements were generally absent, but sometimes muri were beset with granules. Seeds were numerous per capsule, small and non-crateriform. Three types of seed were distinguished based on shape: (1) lenticular with a narrow wing-like margin, (2) trigonous, and (3) globose/subglobose. Trigonous seeds exhibited marked variation in colour, size and shape. On the basis of the pollen and seed characters, used in combination with the type of fruit dehiscence, five natural groups are identified for Nepalese taxa. The generic status of Hedyotis, Oldenlandia and Kohautia is maintained but some species are transferred from Hedyotis to Oldenlandia. Pollen and seed morphology, together with the type of fruit dehiscence, proved to be helpful in delimiting supra- and infrageneric groups within the Hedyotis–Oldenlandia–Kohautia complex.

scholarly journals Pollen grains and seed-morphology as related to Biochemical Patterns in five species of genus Ocimum L (Lamiaceae Juss.) of Saudi Arabia

2017 ◽  
Vol 8 ◽  
pp. 42
Author(s):  
Wael Taha Kasem
Keyword(s):  
Seed Protein ◽  
Pollen Grains ◽  
Seed Morphology ◽  
Protein Profiles ◽  
Electrophoretic Patterns ◽  
Sds Page ◽  
Exine Ornamentation ◽  
Seed Characters ◽  
Molecular Patterns ◽  
Scanning Electron

<p>This study aims to investigate the pollen and seed characters by light microscopy (LM) and scanning electron microscope (SEM) as related to biochemical data of seed protein and esterase isoenzymes by SDS-PAGE technique in the five Saudi Arabian <em>Ocimum</em> L. species. The detailed description for pollen and seed in addition electrophoretic patterns were conducted by means of numerical analyses based on thirty-nine parameters. The pollen grains were zonocolpate, hexacolpate, prolate to subprolate. Three types of exine ornamentation were recognized, perforate, reticulate and granulate. Also,  The periclinal, the anticlinal and the boundaries between cells are described by the aid of SEM which exhibited four main distinct types of nutlets sculpture undulate, quirky, circular and straight. Molecular patterns of protein profiles and esterase (EC.3.1.1.1) showed that esterase could be considered as positive markers than protein, minimum and maximum gene / gene expression of esterase isoenzymes are demonstrated. The relationships between the studied taxa were demonstrated as a phenogram. </p>

scholarly journals Pollen of the Spermacoce (Rubiaceae) species from the Northern Territory of Australia: morphology and taxonomic significance

2005 ◽  
Vol 18 (4) ◽  
pp. 367 ◽  
Author(s):  
S. Dessein ◽  
R. Harwood ◽  
E. Smets ◽  
E. Robbrecht
Keyword(s):  
Light Microscopy ◽  
Morphological Variation ◽  
Pollen Morphology ◽  
Northern Territory ◽  
Taxonomic Significance ◽  
Equatorial Diameter ◽  
Pollen Characters ◽  
Pollen Types ◽  
Electron And Light Microscopy ◽  
Scanning Electron

Complementary to the revision of the genus Spermacoce in the Northern Territory of Australia, pollen morphology of 48 of the 53 native Spermacoce species from the Northern Territory has been investigated by scanning electron and light microscopy. There is considerable variation for most diagnostic pollen characters. The average equatorial diameter (E) ranges from 10.5 to 41.7 µm. Grains are invariably colporate with the apertures situated at the equator (being zonocolporate). The number of apertures varies from 3 to 17. The endoaperture is generally an endocingulum, often with a secondary thinning at the ectocolpus; one species has endocolpi. The sexine is usually perforate, but psilate, foveolate, and (micro)reticulate patterns were also found. Supratectal elements are present as granules or microspines scattered over the whole surface or confined to a region around the ectoapertures. The inner nexine surface is granular, often with irregular grooves (endocracks). The pollen morphological variation observed allows the distinction of four pollen types. Three of these types are not yet recorded in other palynological studies of Spermacoce. Pollen characters are often useful to delimit species and groups of related species.

Taxonomic significance of capsule and seed characters of Indian species of Murdannia Royle (Commelinaceae)

Phytotaxa ◽  
2014 ◽  
Vol 178 (1) ◽  
pp. 1 ◽  
Author(s):  
Ancy Attullil Antony ◽  
Santhosh Nampy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Morphology ◽  
Indian Species ◽  
Taxonomic Significance ◽  
Seed Characters ◽  
Scanning Electron

Capsule and seed morphology of 26 species of Murdannia in India are studied using light microscopy and seeds of 23 species by scanning electron microscopy. The capsules show differences in size, shape and in the number and arrangement of seeds in the locules. The seeds also display considerable variation in size, shape, ornamentation, shape of hilum and position of embryotega. The present study provides useful characters for the delimitation of Indian species of Murdannia.

scholarly journals Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance

2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Infrageneric Classification ◽  
Exine Ornamentation ◽  
Plant Taxon ◽  
Scanning Electron

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)

scholarly journals Características palinológicas de las araliáceas de México

2017 ◽  
pp. 117 ◽  
Author(s):  
Victoria Sosa
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Pollen Grains ◽  
Point Of View ◽  
Generic Level ◽  
Dichotomous Key ◽  
Pollen Characters ◽  
Scanning Electron

This work studies 17 species of mexican Araliaceae from the palynological point of view belonging to the genera Aralia, Dendropanax, Didymopanax, Redera, Oreopanax y Sciadodendron . All the pollen descriptions are presented at the generic level. The grains were studied based in scanning electron microscope and light microscope. It includes a dichotomous key for the genera grains. The results showed few differences between the pollen grains of the genera although some exine characters are important. Redera and Didymopanax presented an exine semi-tectate and the rest of the genera had tectate-perforate grains. This paper compared also some pollen characters with morphological ones, finding pollen characters considered primitive correlated with characters of primitive Araliaceae groups .

Palynological analysis of five selected Onosma taxa

Biologia ◽  
2008 ◽  
Vol 63 (2) ◽  
Author(s):  
Filippo Maggi ◽  
Vladislav Kolarčik ◽  
Pavol Mártonfi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chromosome Number ◽  
Pollen Grains ◽  
Pollen Size ◽  
Palynological Analysis ◽  
Positive Correlation ◽  
Pollen Characters ◽  
Scanning Electron

AbstractPollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5–17.5 × 8.0–14.5 µm), and O. pseudoarenaria the largest grains in average (15.3–20.5 × 12.3–16.3 µm). Pollen grains of all 5 species are small sized, 3-syncolporate, subprolate, heteropolar, with ovate equatorial outlines and circular to rounded triangular polar outlines; the tectum is microechinate. A positive correlation is found between pollen size and chromosome number. The value of pollen characters for taxonomic purposes and the position of the taxa studied within the genus Onosma are discussed.

scholarly journals Palynological studies on some species of Anabasis L. (Amaranthaceae) from Iran

2016 ◽  
Vol 23 (2) ◽  
pp. 247-253 ◽  
Author(s):  
M. Assadi ◽  
S.M.M. Hamdi ◽  
F. Fajani
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Species Delimitation ◽  
Pollen Grains ◽  
Pollen Characters ◽  
Plant Taxon ◽  
Scanning Electron

The paper presents pollen micromorphology by scanning electron microscopy of seven Anabasis L. species namely, A. haussknechtii Bge., A. aphylla L., A. calcarea (Charif & Allen) Bokhari & Wendelbo, A. eugeniae Iljin, A. eriopoda (Shrernk) Volkens, A. annua Bge. and A. setifera Moq. from Iran. Pollen grains in all studied species are peripolyporate, spherical and are of two basic types based on pores diameter inner of holes. The study showed that the sculpturing of exine provides valuable characters in species delimitation, sometimes even for closely related ones. An artificial key based on pollen characters for seven species is provided.Bangladesh J. Plant Taxon. 23(2): 247-253, 2016 (December)

scholarly journals Taxonomic significance of pollen morphology in Succisa and Succisella

2019 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin ◽  
Lyudmila M. Nitsenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Oblate Spheroidal ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Scanning Electron

Abstract Pollen morphology of representatives of the genera Succisa and Succisella in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains in both taxa were tricolpate, prolate, rarely oblate-spheroidal or spheroidal; large-sized. Their outline in polar view was subcircular, rarely trilobate, in equatorial view elliptical or rarely circular. Colpi short, of variable width, margins irregular with distinct, narrow margo, and blunt or acute ends. Exine sculpture was echinate-microechinate. The revealed characteristics of pollen grains are taxonomically significant at the generic and specific levels, and they can be used in pollen analysis. Palynomorphological data are consistent with the results of recent molecular phylogenetic studies. Data on pollen morphology also confirm taxonomic circumscription of Succiseae V. Mayer & Ehrend. Pollen grains in Succiseae are characterized by a tricolpate aperture type, which was probably ancestral in Dipsacaceae s. str.

scholarly journals Palynological analysis of genus Geranium (Geraniaceae) and its systematic implications using scanning electron microscopy

Caryologia ◽  
2021 ◽  
Vol 74 (3) ◽  
pp. 31-43
Author(s):  
Jun Wang ◽  
Qiang Ye ◽  
Tong Zhang ◽  
Xusheng Shi ◽  
Majid Khayatnezhad ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Prolate Spheroid ◽  
Multivariate Statistical ◽  
Pollen Characters ◽  
Pollen Types ◽  
Ward Clustering ◽  
The Relationship ◽  
Scanning Electron

Pollen morphology of 23 species belonging to Geranium have been studied in details, which represent eight sections of two subgenera i.e., G. sect. Dissecta, Geranium, and Tuberosa of subgen. Geranium, Divaricata, Lucida, Ruberta and Trilopha of subgen. Robertium. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Different palyno-morphological features have been observed, and the closely related species were distinguished. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were monad, radially symmetric, isopolar, apertures were tricolporate, and of spheroid, prolate-spheroid or sub-prolate classes. Three pollen types were recognized on the basis of differences in exine sculpturing pattern: reticulate-clavate, striate-rugulate, reticulum cristatum with clavae. Observed differences were not of diagnostic importance in subgenera and sections level. The main objective of this study is to find distinguish pollen characters in the species of the genus Geranium and to elucidate their systematics importance.

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