Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

Zygote ◽  
2010 ◽  
Vol 18 (4) ◽  
pp. 315-321 ◽  
Author(s):  
Yoshikazu Nagao ◽  
Yumiko Harada ◽  
Mari Yamaguchi ◽  
Akane Igarashi ◽  
Yuki Ooshima ◽  
...  
Keyword(s):  
Epigallocatechin Gallate ◽  
Blastocyst Stage ◽  
Blastocyst Formation ◽  
Antioxidant Treatment ◽  
Rate Of Development ◽  
University Of Wisconsin ◽  
Temperature Solution ◽  
Effects Of Temperature ◽  
Reproductive Study ◽  
Wisconsin Solution

SummaryThe overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ovaries by examining the effects of temperature, solution and supplementation. In Experiment 1, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved at 15°C was higher than that at either 5 or 25°C (p < 0.05). In Experiment 2, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved in University of Wisconsin solution was higher than when PBS or saline was used (p < 0.05). In Experiment 3, oocytes preserved in saline supplemented with 0.3 mM glutathione (GSH) exhibited an increase in the rate of blastocyst formation compared with oocytes supplemented with 0 or 3 mM GSH (p < 0.05). In Experiment 4, supplementation with 10 μM epigallocatechin gallate during ovary preservation increased the rate of blastocyst formation (p < 0.05). The blastocysts derived from ovaries stored in saline supplemented with GSH at 15°C for 24 h were shown to develop into normal offsprings following transfer to recipient heifers. Our studies indicate that bovine IVM/IVF embryos derived from ovaries preserved in saline supplemented with an antioxidant at 15°C for 24 h can successfully develop to the blastocyst stage and result in offspring.

Protection of the rat liver against rewarming ischemic injury by University of Wisconsin solution

2001 ◽  
Vol 386 (1) ◽  
pp. 31-37 ◽  
Author(s):  
M. Lutterová ◽  
M. Kukan ◽  
K. Vajdová ◽  
D. Kuba ◽  
C. Mišlanová ◽  
...  
Keyword(s):  
Rat Liver ◽  
Ischemic Injury ◽  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Wisconsin Solution

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Zygote ◽  
2005 ◽  
Vol 13 (4) ◽  
pp. 335-345 ◽  
Author(s):  
Masaki Iwamoto ◽  
Akira Onishi ◽  
Dai-ichiro Fuchimoto ◽  
Tamas Somfai ◽  
Shun-ichi Suzuki ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Blastocyst Stage ◽  
The Other ◽  
Control Group ◽  
Blastocyst Formation ◽  
Cell Nuclei ◽  
Somatic Nucleus ◽  
Membrane Breakdown ◽  
Caffeine Treatment ◽  
M Phase

The possibility of using aged porcine oocytes treated with caffeine, which inhibits the decrease in M-phase promoting factor activity, for pig cloning was evaluated. Cumulus–oocyte complexes (COCs) were cultured initially for 36h and subsequently with or without 5mM caffeine for 24h (in total for 60h: 60CA+ or 60CA– group, respectively). As a control group, COCs were cultured for 48h without caffeine (48CA–). The pronuclear formation rates at 10h after electrical stimulation in the 60CA+ and 60CA– groups decreased significantly (p<0.05) compared with the 48CA– group. However, the fragmentation rate was significantly higher (p<0.05) in the 60CA– group than in the 60CA+ and 48CA– groups. When the stimulated oocytes were cultured for 6 days, the 60CA+ group showed significantly lower blastocyst formation and higher fragmentation or degeneration rates (p<0.05) than the 48CA– group. However, the number of total cells in blastocysts was not affected by maturation period or caffeine treatment. When somatic cell nuclei were injected into the non-enucleated oocytes and exposed to cytoplasm for a certain duration (1–11h) before the completion of maturation (48 or 60h), the rate of nuclear membrane breakdown after exposure to cytoplasm for 1–2h in the 60CA– oocytes was significantly lower (p;<0.05) than in the other experimental groups. The rate of scattered chromosome formation in the same 60CA– group tended to be lower (p=0.08) than in the other groups. After the enucleation and transfer of nuclei, blastocyst formation rates in the 60CA+ and 60CA– groups were significantly lower (p<0.05) than in the 48CA– group. Blastocyst quality did not differ among all the groups. These results suggest that chromosome decondensation of the transplanted somatic nucleus is affected by both the duration of exposure to cytoplasm and the age of the recipient porcine oocytes, and that caffeine treatment promotes nuclear remodelling but does not prevent the decrease in the developmental ability of cloned embryos caused by oocyte aging.

Observations on the free-living stages of the stomach worm Haemonchus contortus

Parasitology ◽  
1963 ◽  
Vol 53 (3-4) ◽  
pp. 469-481 ◽  
Author(s):  
J. H. Rose
Keyword(s):  
Haemonchus Contortus ◽  
Free Living ◽  
Rate Of Development ◽  
Temperature And Humidity ◽  
Survival And Development ◽  
Effects Of Temperature ◽  
Eggs And Larvae ◽  
Heavy Mortality

Outdoor observations were made on the rate of development of the free-living stages of H. contortus at different times of the year, and on the migratory activities and longevity of the larvae in faeces, on herbage and in soil.The effects of temperature and humidity on the survival and development of eggs and larvae, both in faeces and when separated from the faeces, were studied in the laboratory.The results of these observations are discussed in relation to the heavy mortality of the free-living stages of H. contortus and to the transmission of infection in the field.

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