Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Summary This study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

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Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage

Theriogenology ◽

10.1016/s0093-691x(02)00953-6 ◽

2002 ◽

Vol 58 (7) ◽

pp. 1349-1360 ◽

Cited By ~ 45

Author(s):

Li-Jun Huo ◽

Xing-Hong Ma ◽

Zeng-Ming Yang

Keyword(s):

Mitochondrial Activity ◽

Sperm Viability ◽

Long Term Storage ◽

Boar Semen ◽

Term Storage

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EVIDENCE AND POSSIBLE MECHANISMS FOR MEMBRANE DETERIORATION DURING LONG-TERM STORAGE OF POTATO

HortScience ◽

10.21273/hortsci.25.9.1127e ◽

1990 ◽

Vol 25 (9) ◽

pp. 1127e-1127

Author(s):

Kumar G.N. Mohan ◽

N.R. Knowles

Keyword(s):

Membrane Damage ◽

Membrane Integrity ◽

Growth Potential ◽

Potato Seed ◽

Solute Leakage ◽

Long Term Storage ◽

Mda Content ◽

Term Storage ◽

Membrane Deterioration

Studies on the mechanisms by which growth potential of potato seed-tubers declines during aging suggest that membrane deterioration may be involved. Malondialdehyde (MDA) content, ethane evolution, solute leakage, and activity of the membrane-bound ethylene forming enzyme (EFE) were measured in tissues from 2, 14 and 26-month-old potato tubers as potential indicators of peroxidative damage and loss in membrane integrity. Solute leakage increased with tissue age, reflecting loss in membrane integrity. MDA content, a measure of lipid peroxidation, also increased with tuber age. Ethane is a product of free-radical-mediated peroxidation of polyunsaturated fatty acids (PUFA), and is therefore a sensitive marker of membrane damage. In the absence of fatty acid substrate, old tissue evolved less ethane than young tissue. However, addition of linoleate to the incubation medium stimulated more ethane from the oldest tissue, indicating a higher potential for ethane production. In vivo conversion of ACC to ethylene by EFE declined with age, possibly due to membrane deterioration. These studies show that peroxidation of PUFA may be influencing membrane integrity during long-term storage of potato.

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EFFECT OF VARIOUS CRYOPROTECTANTS ON PRESERVATION OF BLACK TIGER Penaeus monodon SHRIMP SPERMATOZOA

Indonesian Aquaculture Journal ◽

10.15578/iaj.15.2.2020.77-83 ◽

2021 ◽

Vol 15 (2) ◽

pp. 77

Author(s):

Rosmiati Rosmiati ◽

Ike Trismawanti ◽

Samuel Lante

Keyword(s):

Saline Solution ◽

Penaeus Monodon ◽

Sperm Viability ◽

Electrical Shock ◽

Long Term Storage ◽

Tiger Shrimp ◽

Time Period ◽

And Control ◽

Term Storage

The development of cryopreservation technique on tiger shrimp Penaeus monodon broodstock spermatophore has been carried out to support the artificial insemination. This study aims to determine the effect of three cyoprotectants (methanol, dimetylsulphoxide (DMSO), and glycerol) for long term storage of tiger shrimp Penaeus monodon spermatozoa. Spermatophores were collected from the wild broodstocks through electrical shock. Spermatozoa were obtained by homogenizing the spermatophores using a Radnoti micro homogenizer in Ca-free saline solution containing one of three cryoprotectans (methanol, dimetylsulphoxide, and glycerol) separately at the concentration of 5%. One mL of each cryoprotectant containing spermatozoa with the density of 1.02 x 106 cell/mL was transferred into a cryovial and cryopreserved at room temperature, -20°C and -196°C for 5, 10, and 30 days. The apparent sperm viability (ASV) of cryopreserved spermatozoa was monitored after treated. Thawing of cryopreserved spermatozoa was carried out in a 30°C wáter bath for two minutes. The result showed that the best apparent sperm viability was obtained at the using of glycerol at -196°C in liquid nitrogen, even after the thirty days of cryopreservation time period with the ASV of 0.82 x 106 cells/mL (80.39%). Meanwhile two other cryoprotectans displayed the ASV of 0.54 x 106 cells/mL (56.86%), and 0.23 x 106 cells/mL (22.55%). for DMSO and methanol, respectively. In turn, the control showed the lowest ASV with the ASV of 0.01 x 106 cells/mL (1.27%). The ASV showed by this glycerol exhibited asignificant difference (P<0.05) to that of methanol, DMSO, and control.

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Conservation of feline semen: Part I: Cooling and freezing protocols

Journal of Feline Medicine and Surgery ◽

10.1016/s1098-612x(03)00029-9 ◽

2003 ◽

Vol 5 (4) ◽

pp. 203-208 ◽

Cited By ~ 13

Author(s):

G.C Luvoni ◽

E Kalchschmidt ◽

S Leoni ◽

C Ruggiero

Keyword(s):

Assisted Reproduction ◽

Artificial Insemination ◽

Domestic Cat ◽

Accurate Data ◽

Domestic Cats ◽

Breeding Programs ◽

Long Term Storage ◽

Wild Felids ◽

Term Storage

There has been increased interest recently in the conservation of wild felids and preservation of valuable cat breeds. Assisted reproduction, by means of artificial insemination (AI), is an important tool for developing breeding programs for conservation. Optimal use of AI requires accurate data on semen conservation protocols and its long-term storage/survival. In this paper, semen cooling and freezing processes are described, with special emphasis on the results obtained in experiments performed in the domestic cat. Conception rates after AI in wild and domestic cats are also reported.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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