Fluorescence Microscope and Electron-Microscope Studies on the Effect of Trenimon on Human Tumour Cells

1968 ◽  
Vol 17 (1) ◽  
pp. 122-144
Author(s):  
R. Thom
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Local Treatment ◽  
Tumour Cells ◽  
Human Tumour Cells ◽  
Cell Vitality ◽  
Contrast Microscopy ◽  
Nucleolar Component ◽  
Nucleolar Rna ◽  
Supravital Staining

SummaryA report is given on various forms of administration of Trenimon for local treatment of neoplasms of the serous membranes. For supervision and adjustment of the chemotherapeutic effect supravital staining with acridin orange and phase-contrast microscopy of the untreated effusion sediment are especially suitable. This facilitates distinction between newly formed and preformed granules, which is of importance for the diagnosis. Inhibition of dye-induced production of granules is a very sensitive criterium for assessment of cell vitality. The development of regressive changes in the tumour cells caused by chemotherapy is discussed with reference to micrographs of a metastatic pleural sarcomatosis. Electron-microscope studies on the exudate suggest that prior to necrobiosis an increased transfer of the granular nucleolar component into the cytoplasm takes place. This may be caused by a true increase in the transfer of nucleolar RNA towards the cytoplasm or by an inhibition in the late S-phase.

Nile Blue Inducible Fluorescence of Tumour Cells

1972 ◽  
Vol 27 (11) ◽  
pp. 1395-1398 ◽  
Author(s):  
A. L. Bastos ◽  
Dante Marques
Keyword(s):  
Phase Contrast ◽  
Unsaturated Fatty Acids ◽  
Nile Blue ◽  
Tumour Cells ◽  
Phase Contrast Microscopy ◽  
Cytoplasmic Granules ◽  
Deep Blue ◽  
Contrast Microscopy ◽  
Thiazine Dyes ◽  
Molecular Nature

An oxazine dye, Nile Blue sulfate, induces a fluorescent reaction in cytoplasmic granules (NBIFG) of living or fixed tumour cells in the same manner as reported before for Thiazine dyes.The NBIFG correspond to phase contrast positive granules when cells are viewed by phase contrast microscopy. Fluorescence disappears from NBIFG in a matter of 2—4 days and the bodies turn deep blue. These granules have a succinate dehydrogenase (SDG) activity and are negative for acid phosphatase, peroxidasic activity and porphyrin.The cytological findings support the assumption that Nile Blue sulfate forms a salt linkage with unsaturated fatty acids of NBIFG which also show an oxi-reductive activity. The molecular nature of the fluorophore (s) is (are) unknown.

scholarly journals Studies on Schmauch Bodies. I. The Incidence in Normal Cats (Felis Domestica) and the Morphologic Relationship to Heinz Bodies

Blood ◽  
1965 ◽  
Vol 25 (6) ◽  
pp. 999-1008 ◽  
Author(s):  
Anica Sirec ◽  
Marija Sondić ◽  
TIHOMIL BERITIĆ
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Heinz Bodies ◽  
Morphologic Characteristics ◽  
Wide Range ◽  
Contrast Microscopy ◽  
Supravital Staining

Abstract Inclusions in the erythrocytes of normal cats, called Schmauch bodies, have been demonstrated by means of phase contrast microscopy and supravital staining. A very wide range of their incidence, but no correlation with the age of animals has been noted. By their identical staining reactions and common morphologic characteristics Schmauch and Heinz bodies are quite similar, although they might be produced by quite different mechanisms.

Compound sensilla in monogenean skin parasites

Parasitology ◽  
1969 ◽  
Vol 59 (3) ◽  
pp. 625-636 ◽  
Author(s):  
Kathleen M. Lyons
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Phase Contrast ◽  
The Other ◽  
Phase Contrast Microscopy ◽  
Sense Organs ◽  
Dense Membrane ◽  
Contrast Microscopy ◽  
Membrane Bound

The fine structure of two kinds of compound presumed sense organs from the heads of three skin parasitic monogeneans Gyrodactylus sp. Entobdella soleae (larva only) and Acanthocotyle elegans is described. One kind of compound receptor consists of a number of associated sensilla, each ending in a single cilium (the spike sensilla of Gyrodactylus and the cone sensilla of E. soleae oncomiracidium).The other kind of compound organ is made up of one or a few neurones only, each of which bears many cilia (pit organs of E. soleae oncomiracidium and feeding organ sensilla of Acanthocotyle elegans). The spike sensilla of Gyrodactylus have also been studied using a Cambridge Instrument Co. Stereoscan electron microscope and by phase-contrast microscopy. The ciliary endings of all these sense organs are highly modified and have lost the 9 + 2 structure, being packed with many fibres. The fibre arrangement in the cilia of the cone sensillae of E. soleae oncomiracidium and the feeding organ sensilla of A. elegans has been compared with that in the ciliary endings of other invertebrate mechano- and chemoreceptors. The possibility that the spike sensilla of Gyrodactylus may be chemoreceptors has been discussed but it is considered premature to attempt to assign functions to the other sense organs studied. Electron dense membrane-bound inclusions occurring specifically in the nerves supplying the spike sensilla of Gyrodactylus may be neurosecretory.

Description of the immatures of the predaceous midge Bezzia blantoni Spinelli & Wirth (Diptera: Ceratopogonidae)

Zootaxa ◽  
2009 ◽  
Vol 2295 (1) ◽  
pp. 46-54 ◽  
Author(s):  
MARIA M. RONDEROS ◽  
GUSTAVO R. SPINELLI
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Instar Larva ◽  
Fourth Instar ◽  
Phase Contrast Microscopy ◽  
Fourth Instar Larva ◽  
Contrast Microscopy ◽  
Breeding Location ◽  
First Time ◽  
Scanning Electron

The fourth instar larva and pupa of Bezzia blantoni Spinelli & Wirth (1989) are described, illustrated and photomicrographed by using phase-contrast microscopy and Scanning Electron Microscope (SEM). The larva, which shows features typical to carnivorous larvae, is compared with that of B. roldani Spinelli & Wirth. The species is recorded for the first time from the Martín García island, located in the La Plata river between Argentina and Uruguay. Details of the biology traits, breeding location and feeding behavior are given.

Sense organs of monogenean skin parasites ending in a typical cilium

Parasitology ◽  
1969 ◽  
Vol 59 (3) ◽  
pp. 611-623 ◽  
Author(s):  
Kathleen M. Lyons
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Research Council ◽  
Sense Organ ◽  
Science Research ◽  
Sense Organs ◽  
Marine Biological Laboratory ◽  
Sensory Neurone ◽  
Research Fellowship ◽  
Contrast Microscopy

Single receptors seen with the electron microscope to consist of a terminal cilium embedded in a nerve bulb have been found in the skin parasitic monogenean Gyrodactylus sp., adult and larval Entobdella soleae, Leptocotyle minor (adult) and in the endoparasitic juvenile of Amphibdella flaviolineata. Their distribution in Gyrodactylus sp. has been mapped using phase-contrast microscopy, and staining with the indoxyl acetate method for non-specific esterases revealed a nervous connexion between the lateral ventral nerve cord and the ‘tangoreceptor’. The general relationships of the nervous system in Gyrodactylus were investigated by staining with the thiocholine method for cholinesterase. The ‘sensory’ neurone contains vesicles, microtubules and mitochondria and the whole nerve bulb is sealed into the epidermis by means of septate desmosomes. The basal body of the cilium is not greatly modified and there is no obvious rootlet system. The terminal cilium of single receptors in Entobdella soleae has a 9 + 2 structure. A compound sense organ from the head of Entobdella soleae has also been described. The significance of the fibre arrangement in the terminal cilia and the possible roles of these sense organs have been discussed.I should like to thank Dr D. L. Lee for training in electron microscope techniques and for his encouragement, Dr P. Tate for his continued interest and the staff of the Marine Biological Laboratory, Plymouth, especially Mr G. Best and Mr J. E. Green, for their assistance. This work was conducted during tenure of a Science Research Council Fellowship and the Tucker-Price Research Fellowship of Girton College.

scholarly journals THE CYTOLOGY OF RICKETTSIAE

1949 ◽  
Vol 89 (6) ◽  
pp. 681-686 ◽  
Author(s):  
Hans Ris ◽  
John P. Fox
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Cellular Organization ◽  
Desoxyribonucleic Acid ◽  
Internal Structures ◽  
Basic Dyes ◽  
Contrast Microscopy ◽  
Nuclear Structures ◽  
Clear Differentiation

Internal structures of rickettsiae seen with phase contrast microscopy and in the electron microscope contain desoxyribonucleic acid and are therefore nuclear structures similar to those found in bacteria. They are minute spherical bodies, either single as in spherical rickettsiae or varying in number from 2 to 4 in rod-shaped forms. Occasional dumbbell-shaped chromatinic bodies are thought to represent these structures in the process of division. The presence of ribonucleic acid in the cytoplasm of rickettsiae was demonstrated with the use of ribonuclease and basic dyes. Rickettsiae therefore have a cellular organization similar to that of certain bacteria, with a clear differentiation into nuclear structure and cytoplasm.

Observation of biological macromolecules using various electron microscopic methods

1978 ◽  
Vol 36 (2) ◽  
pp. 170-171
Author(s):  
Mitsuo Ohtsuki ◽  
Michael Sogard
Keyword(s):  
Electron Microscope ◽  
Phase Contrast ◽  
Image Interpretation ◽  
Density Lipoprotein ◽  
Biological Macromolecules ◽  
Contrast Effects ◽  
Biological Structure ◽  
Electron Microscopic ◽  
Structural Investigations ◽  
Staining Techniques

Structural investigations of biological macromolecules commonly employ CTEM with negative staining techniques. Difficulties in valid image interpretation arise, however, due to problems such as variability in thickness and degree of penetration of the staining agent, noise from the supporting film, and artifacts from defocus phase contrast effects. In order to determine the effects of these variables on biological structure, as seen by the electron microscope, negative stained macromolecules of high density lipoprotein-3 (HDL3) from human serum were analyzed with both CTEM and STEM, and results were then compared with CTEM micrographs of freeze-etched HDL3. In addition, we altered the structure of this molecule by digesting away its phospholipid component with phospholipase A2 and look for consistent changes in structure.

The Imaging of Crystal Structures and Crystal Defects

1978 ◽  
Vol 36 (3) ◽  
pp. 207-217
Author(s):  
J.M. Cowley
Keyword(s):  
Electron Microscope ◽  
Crystal Structures ◽  
Phase Contrast ◽  
Crystal Defects ◽  
Atom Density ◽  
Lack Of Information ◽  
Spatial Frequencies

The problem of "understandinq" electron microscope imaqes becomes more acute as the resolution is improved. The naive interpretation of an imaqe as representinq the projection of an atom density becomes less and less appropriate. We are increasinqly forced to face the complexities of coherent imaqinq of what are essentially phase objects. Most electron microscopists are now aware that, for very thin weakly scatterinq objects such as thin unstained bioloqical specimens, hiqh resolution imaqes are best obtained near the optimum defocus, as prescribed by Scherzer, where the phase contrast imaqe qives a qood representation of the projected potential, apart from a lack of information on the lower spatial frequencies. But phase contrast imaqinq is never simple except in idealized limitinq cases.

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