Ultrathin Sectioning of Fixed but Unembedded Tissue

1980 ◽  
Vol 38 ◽  
pp. 650-653
Author(s):  
Daniel C. Pease
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Serum Albumin ◽  
Cell Types ◽  
Progress Report ◽  
Fixed Tissue ◽  
Biological Objects ◽  
Transmission Electron ◽  
Isolated Chloroplasts

This is intended as a progress report to demonstrate that it is feasible within limits to section unembedded, glutaraldehyde-fixed tissue thinly enough for transmission electron microscopy. Much cytological detail will be preserved. The work has been predicated upon a demonstration of J.L. Farrant and J.D. McLean1 that x-linking concentrated serum albumin gel with glutaraldehyde can produce an adequate support for sectioning small biological objects such as erythrocytes, small algae, bacteria, and mitochondria. Subsequently, G.L. Nicolson2 utilized the same technique to obtain interesting sections of isolated chloroplasts. These experiments suggested to me that the proteins of the cytosol of many cell types might also be adequately x-linked with glutaraldehyde to permit ultrathin sectioning if the specimens could otherwise be suitably supported.

Simplified procedures for releasing and concentrating microorganisms from soil for transmission electron microscopy viewing as thin-sectioned and frozen-etched preparations

1975 ◽  
Vol 21 (3) ◽  
pp. 252-262 ◽  
Author(s):  
D. L. Balkwill ◽  
D. P. Labeda ◽  
L. E. Casida Jr.
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Types ◽  
Soil Slurry ◽  
Thin Sections ◽  
Choice Of Procedure ◽  
Transmission Electron ◽  
Cell Release ◽  
Simplified Procedures ◽  
Simplified Procedure

A simplified procedure is presented for releasing and concentrating indigenous microbial cells from soil for viewing by transmission electron microscopy as thin sections or replicas of frozen-etched preparations. This procedure is compared with two others reported earlier, and their relative merits are discussed as concerns the choice of procedure for the cellular information desired from the soil. Freeze-etching showed that the cell types and size distributions for cells which have been released and concentrated from soil are in general agreement with those for cells in a crude soil slurry in which no attempt to release and concentrate cells was made. Microcolonies were present both in the crude slurry and in the discard soil debris centrifugation pellets from the cell release and concentration procedures. In contrast to the historic assumptions, these microcolonies, as well as some individual cells embedded in soil debris could not be broken up and (or) dislodged so that they would be washed from the soil. The relative numbers of these cells remaining with the soil debris, however, could not be quantitated in the present study.

scholarly journals The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments.

1990 ◽  
Vol 171 (2) ◽  
pp. 401-413 ◽  
Author(s):  
V Ley ◽  
E S Robbins ◽  
V Nussenzweig ◽  
N W Andrews
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Trypanosoma Cruzi ◽  
Ammonium Chloride ◽  
Protozoan Parasite ◽  
Cell Types ◽  
Immunocytochemical Localization ◽  
Transmission Electron ◽  
Acidic Compartments ◽  
Vacuolar Ph

The protozoan parasite Trypanosoma cruzi can infect many distinct mammalian cell types. The parasites enter cells through the formation of phagocytic vacuoles, but later are found free in the cytosol, where they multiply as amastigotes. Using transmission electron microscopy we found that within 2 h after infection 70% of the parasites, including examples of both mammalian forms (trypomastigotes and amastigotes), were inside partially disrupted vacuoles or free in the cytosol. We demonstrated that the pH of vacuoles containing recently interiorized parasites is acidic, through immunocytochemical localization of the acidotropic compound DAMP (18) in their interior. Increasing the vacuolar pH with chloroquine, ammonium chloride, methylamine, or monensin significantly inhibited the escape of the parasites into the cytosol. These results are compatible with the hypothesis that an acid-active hemolysin of T. cruzi (15) might be involved in the escape mechanism.

Characterization of mammary cells from the lactating rat by electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 560-561
Author(s):  
P. Sadhukhan ◽  
J. Chakraborty ◽  
M. S. Soloff ◽  
M. H. Wieder ◽  
D. Senitzer
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Types ◽  
Myoepithelial Cells ◽  
Mammary Tissue ◽  
Secretory Cells ◽  
Isolated Cells ◽  
Transmission Electron ◽  
Cell Processes ◽  
Phosphatase Cytochemistry

The means to identify cells isolated from the mammary gland of the lactating rat as a prerequisite for cell purification have been developed.The cells were isolated from mammary tissue with 0. 1% collagenase, and they were visualized by scanning and transmission electron microscopy and by alkaline phosphatase cytochemistry.The milk-secreting cells have surface microvilli, whereas the surface of the myoepithelial cells is smooth (Fig. 1). The two isolated epithelial cell types are readily distinguishable by transmission electron microscopy (Fig. 2). The secretory cells contain vacuoles and a relatively extensive rough endoplasmic reticulum, whereas the myoepithelial cells contain a more osmiophilic cytoplasm, contractile filaments (Fig. 3) and elongate processes. These features are consistent with the appearance of the two cell types in situ.Incubation of isolated cells with oxytocin prior to glutaraldehyde fixation resulted in the contraction of the myoepithelial cell processes (Figs. 4 & 5). This physiological response to oxytocin shows that the isolated myoepithelial cells were intact. The appearance of isolated secretory cells was unchanged by the presence of oxytocin.

scholarly journals Analysis by Light, Scanning, and Transmission Microscopy of the Intima Synovial of the Temporomandibular Joint of Human Fetuses during the Development

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Carlos Sabu Alvez ◽  
Luis Otavio Carvalho de Moraes ◽  
Sergio R. Marques ◽  
Roberto C. Tedesco ◽  
Leandro J. C. Harb ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Temporomandibular Joint ◽  
Cell Types ◽  
Human Fetuses ◽  
Transmission Microscopy ◽  
Joint Cavity ◽  
Transmission Electron ◽  
Intima Layer

Objective. To characterize morphologically and ultrastructurally using light microscopy, the scanning electron microscopy and transmission electron microscopy the intima synovial of the temporomandibular joint (TMJ) of human fetuses between the 10th and the 38th week of development. Materials and Methods. The TMJ was dissected bilaterally in 37 human fetuses belonging to the Institute of Embryology of the University Complutense of Madrid and of the Federal University of São Paulo. Results. The outcome by light microscopy showed the morphology of the TMJ and that the formation of inferior joint cavity precedes the superior joint cavity and the presence of blood vessels in the synovial. Conclusion. By scanning and transmission electron microscopy we observed the presence of two well-defined cell types in the intima layer of synovial of the TMJ of human fetuses, macrophage-like type A cell and fibroblast-like type B cell, and the presence of the a third cell type, defined by the name of intermediate lining cell in the intima layer of the synovial.

The Relationship Between Pit Membrane Ultrastructure and Chemical Impregnability of Wood

Holzforschung ◽  
1999 ◽  
Vol 53 (4) ◽  
pp. 341-346 ◽  
Author(s):  
Adya Singh ◽  
Bernard Dawson ◽  
Robert Franich ◽  
Faye Cowan ◽  
Jeremy Warnes
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Cell Types ◽  
Transmission Electron ◽  
Pit Membrane ◽  
Before And After ◽  
Ray Cells ◽  
Membrane Ultrastructure ◽  
The Relationship

Summary The woods of Alder and Eucalypt were examined by light microscopy before and after a chemical treatment by the Indurite process to increase the hardness of the wood. The pattern of wood cell impregnation for Alder differed significantly from Eucalypt in some respects. In Alder wood all cell types eg. vessels, fibres and rays, were impregnated in similar proportions. In comparison, in Eucalypt wood the impregnation material was largely confined to ray cells and the lumina of vessels; other cell types were either not impregnated or impregnated in very small numbers. Transmission electron microscopy of Alder and Eucalypt woods suggests that ultrastructural differences in the texture and porosity of pit membranes may be the main reason for the observed differences between these wood species with regard to their impregnability by the impregnation material used.

scholarly journals Development of epididymis and vas deferens in Galea spixii: an ultrastructural view

2016 ◽  
Vol 33 (03) ◽  
pp. 131-137
Author(s):  
P. Santos ◽  
M. Arroyo ◽  
M. Oliveira ◽  
M. Miglino ◽  
A. Assis Neto
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sexual Development ◽  
Vas Deferens ◽  
Cell Types ◽  
Testicular Development ◽  
Morphological Development ◽  
Basal Cells ◽  
Transmission Electron

AbstractThe study aimed to characterize the ultrastructure of components of the sperm pathway in Galea spixii (Spix's yellow-toothed cavy) at different stages of sexual development. Segments of the epididymis and vas deferens of 10 Galea spixii were studied using scanning and transmission electron microscopy. Principal and basal cells with similar characteristics were seen in the epididymis and vas deferens. Both cell types are responsible for maturation of sperm as well as the ability to fertilize and reabsorption. The post natal morphological development of the epididymis and vas deferens was similar to testicular development of the species.

Effects of the substratum on the migration of primordial germ cells

1982 ◽  
Vol 299 (1095) ◽  
pp. 177-183 ◽  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Migration ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Cell Types ◽  
Artificial Substrates ◽  
Transmission Electron ◽  
Migratory Cells

It is now clear from work on defined cell types on artificial substrates that various chemical and physical inhomogeneities in the substrates can guide cell locomotion. It is also becoming clear that less well defined inhomogeneities in living cell substrates can guide the normal locomotion of embryonic migratory cells in vivo. The primordial germ cells (p.g.cs) of early anuran amphibian embryos are proving a useful model for the study of cell migration. When isolated from the embryo and cultured on living cellular substrate, p.g.cs become oriented by the shapes of the underlying cells or by their stress fibre cytoskeleton, or both. A combination of scanning and transmission electron microscopy in vivo shows a clearly aligned cellular substrate for p.g.c. migration along part of their route. Furthermore, we find that the glycoprotein fibronectin is involved in p.g.c. adhesion, which suggests a link between orientation of the substrate cells and p.g.c. guidance.

Ultrastructure of Solitary Enchondromas

1984 ◽  
Vol 9 (1) ◽  
pp. 95-97 ◽  
Author(s):  
MARILYN L. ZIMNY ◽  
I. REDLER
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Cell Types ◽  
Transmission Electron ◽  
The Matrix ◽  
Small Bones ◽  
Dying Cells ◽  
Myelin Figures

Solitary enchondromas obtained from the small bones of the hand were studied with transmission electron microscopy. Three cell types were seen as follows: (1) young looking, active cells with extensive dilated rough endoplasmic reticulum and well defined Golgi and mitochondria; (2) older looking, degenerating cells with dilated rough endoplasmic reticulum, well defined Golgi, glycogen masses, vacuoles containing tropocollagen, lipid and myelin figures; and (3) dying cells showing loss of cell membrane and lysosomal-like bodies. A young chondroblastic cell may try to mature, become a normal chondrocyte that produces normal matrix but it does not succeed and dies. Enchondromal cells are not capable of forming tropocollagen or synthesizing proteoglycans for the matrix.

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