Effects of Natural Radiation, Photosynthetically Active Radiation and Artificial Ultraviolet Radiation-B on the Chloroplast Organization and Metabolism ofPorphyra acanthophoravar.brasiliensis(Rhodophyta, Bangiales)

2012 ◽  
Vol 18 (6) ◽  
pp. 1467-1479 ◽  
Author(s):  
Zenilda L. Bouzon ◽  
Fungyi Chow ◽  
Carmen S. Zitta ◽  
Rodrigo W. dos Santos ◽  
Luciane C. Ouriques ◽  
...  
Keyword(s):  
Ultraviolet Radiation ◽  
Photosynthetically Active Radiation ◽  
Laser Scanning ◽  
Reductase Activity ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ambient Conditions ◽  
Natural Radiation ◽  
Active Radiation ◽  
Ultraviolet Radiation B

AbstractWe undertook a study ofPorphyra acanthophoravar.brasiliensisto determine its responses under ambient conditions, photosynthetically active radiation (PAR), and PAR+UVBR (ultraviolet radiation-B) treatment, focusing on changes in ultrastructure, and cytochemistry. Accordingly, control ambient samples were collected in the field, and two different treatments were performed in the laboratory. Plants were exposed to PAR at 60 μmol photons m−2s−1and PAR + UVBR at 0.35 W m−2for 3 h per day during 21 days ofin vitrocultivation. Confocal laser scanning microscopy analysis of the vegetative cells showed single stellate chloroplast in ambient and PAR samples, but in PAR+UVBR-exposed plants, the chloroplast showed alterations in the number and form of arms. Under PAR+UVBR treatment, the thylakoids of the chloroplasts were disrupted, and an increase in the number of plastoglobuli was observed, in addition to mitochondria, which appeared with irregular, disrupted morphology compared to ambient and PAR samples. After UVBR exposure, the formation of carpospores was also observed. Plants under ambient conditions, as well as those treated with PAR and PAR+UVBR, all showed different concentrations of enzymatic response, including glutathione peroxidase and reductase activity. In summary, the present study demonstrates thatP. acanthophoravar.brasiliensisshows the activation of distinct mechanisms against natural radiation, PAR and PAR+UVBR.

scholarly journals Enhanced Grain-boundary Emission Lifetime and Additive Induced Crystal Orientation in One-Step Spin-Coated Mixed Cationic (FA/MA) Lead Perovskite Thin Films Stabilized by Zinc Iodide Doping

2017 ◽  
Author(s):  
René M. Williams ◽  
Loreta A. Muscarella ◽  
Dina Petrova ◽  
Rebecca Jorge Cervasio ◽  
Aram Farawar ◽  
...  
Keyword(s):  
Thin Films ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Crystal Formation ◽  
Ambient Conditions ◽  
Emission Lifetime ◽  
Photoluminescence Lifetime ◽  
One Step ◽  
Zinc Iodide

Mixed cationic lead perovskites containing formamidinium and methylammonium can be stabilized by incorporating ZnI<sub>2 </sub>as an “internal desiccant”. Next to prolonged stability under ambient conditions we show with XRD that the use of an additive, 3-chloropropyl ammonium chloride, influences crystal formation by orienting the crystals. These ~500 nm crystals show individual photoluminescent behavior in thin films and have a longer photoluminescence lifetime at the grain boundaries as compared to the center of the crystal or relative to un-doped materials without the additive made under identical conditions. Charges recombine slower at the edges of the crystals as observed with confocal laser scanning microscopy

scholarly journals Enhanced Grain-boundary Emission Lifetime and Additive Induced Crystal Orientation in One-Step Spin-Coated Mixed Cationic (FA/MA) Lead Perovskite Thin Films Stabilized by Zinc Iodide Doping

2017 ◽  
Author(s):  
Loreta A. Muscarella ◽  
Dina Petrova ◽  
Rebecca Jorge Cervasio ◽  
Aram Farawar ◽  
Olivier Lugier ◽  
...  
Keyword(s):  
Thin Films ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Crystal Formation ◽  
Ambient Conditions ◽  
Emission Lifetime ◽  
Photoluminescence Lifetime ◽  
One Step ◽  
Zinc Iodide

Mixed cationic lead perovskites containing formamidinium and methylammonium can be stabilized by incorporating ZnI<sub>2 </sub>as an “internal desiccant”. Next to prolonged stability under ambient conditions we show with XRD that the use of an additive, 3-chloropropyl ammonium chloride, influences crystal formation by orienting the crystals. These ~500 nm crystals show individual photoluminescent behavior in thin films and have a longer photoluminescence lifetime at the grain boundaries as compared to the center of the crystal or relative to un-doped materials without the additive made under identical conditions. Charges recombine slower at the edges of the crystals as observed with confocal laser scanning microscopy. The material can also be prepared as a black precursor powder by a solid-solid reaction under ambient conditions.

scholarly journals Enhanced Grain-boundary Emission Lifetime and Additive Induced Crystal Orientation in One-Step Spin-Coated Mixed Cationic (FA/MA) Lead Perovskite Thin Films Stabilized by Zinc Iodide Doping

2017 ◽  
Author(s):  
Loreta A. Muscarella ◽  
Dina Petrova ◽  
Rebecca Jorge Cervasio ◽  
Aram Farawar ◽  
Olivier Lugier ◽  
...  
Keyword(s):  
Thin Films ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Crystal Formation ◽  
Ambient Conditions ◽  
Emission Lifetime ◽  
Photoluminescence Lifetime ◽  
One Step ◽  
Zinc Iodide

Mixed cationic lead perovskites containing formamidinium and methylammonium can be stabilized by incorporating ZnI<sub>2 </sub>as an “internal desiccant”. Next to prolonged stability under ambient conditions we show with XRD that the use of an additive, 3-chloropropyl ammonium chloride, influences crystal formation by orienting the crystals. These ~500 nm crystals show individual photoluminescent behavior in thin films and have a longer photoluminescence lifetime at the grain boundaries as compared to the center of the crystal or relative to un-doped materials without the additive made under identical conditions. Charges recombine slower at the edges of the crystals as observed with confocal laser scanning microscopy. The material can also be prepared as a black precursor powder by a solid-solid reaction under ambient conditions.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Use of confocal laser scanning microscopy and a computer model to understand ink cavitation and filamentation

TAPPI Journal ◽  
2010 ◽  
Vol 9 (10) ◽  
pp. 7-15
Author(s):  
HANNA KOIVULA ◽  
DOUGLAS BOUSFIELD ◽  
MARTTI TOIVAKKA
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Print Quality ◽  
Length Scale ◽  
Offset Printing ◽  
Significant Difference ◽  
Printing Speed

In the offset printing process, ink film splitting has an important impact on formation of ink filaments. The filament size and its distribution influence the leveling of ink and hence affect ink setting and the print quality. However, ink filaments are difficult to image due to their short lifetime and fine length scale. Due to this difficulty, limited work has been reported on the parameters that influence filament size and methods to characterize it. We imaged ink filament remains and quantified some of their characteristics by changing printing speed, ink amount, and fountain solution type. Printed samples were prepared using a laboratory printability tester with varying ink levels and operating settings. Rhodamine B dye was incorporated into fountain solutions to aid in the detection of the filaments. The prints were then imaged with a confocal laser scanning microscope (CLSM) and images were further analyzed for their surface topography. Modeling of the pressure pulses in the printing nip was included to better understand the mechanism of filament formation and the origin of filament length scale. Printing speed and ink amount changed the size distribution of the observed filament remains. There was no significant difference between fountain solutions with or without isopropyl alcohol on the observed patterns of the filament remains.

ACTIVATED SLUDGE FLOC CHARACTERIZATION BY CONFOCAL LASER SCANNING MICROSCOPY

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

Cuticular structures in the copulatory apparatus of Planorbis planorbis (Linnaeus, 1758) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (1) ◽  
pp. 11-16
Author(s):  
E.V. Soldatenko ◽  
A.A. Petrov
Keyword(s):  
Light Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Taxonomic Status ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Copulatory Apparatus ◽  
The Family ◽  
Cuticular Structures

The morphology of the copulatory apparatus and associated cuticular structures in Planorbis planorbis was studied by light microscopy, SEM, TEM and confocal laser scanning microscopy. The significance of these cuticular structures for the taxonomic status of the species and for the systematics of the family Planorbidae in general is discussed.

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