scholarly journals Liquid In-situ Transmission Electron Tomography Using Hitachi HILEM IL1000 TM Ionic Liquid

2016 ◽  
Vol 22 (S3) ◽  
pp. 810-811 ◽  
Author(s):  
James P. Kilcrease ◽  
Edgar Voelk
Keyword(s):  
Ionic Liquid ◽  
Electron Tomography ◽  
Transmission Electron

Cryo-transmission electron microscopy of Ag nanoparticles grown on an ionic liquid substrate

2010 ◽  
Vol 25 (7) ◽  
pp. 1264-1271 ◽  
Author(s):  
Dalaver H. Anjum ◽  
Rebecca M. Stiger ◽  
James J. Finley ◽  
James F. Conway
Keyword(s):  
Electron Microscopy ◽  
Ionic Liquid ◽  
Electron Tomography ◽  
Silver Nanostructures ◽  
Electron Dose ◽  
Transmission Electron ◽  
Induced Motion ◽  
Novel Method ◽  
Individual Nanoparticles

We report a novel method of growing silver nanostructures by cathodic sputtering onto an ionic liquid (IL) and our visualization by transmission cryo-electron microscopy to avoid beam-induced motion of the nanoparticles. By freezing the IL suspension and controlling electron dose, we can assess properties of particle size, morphology, crystallinity, and aggregation in situ and at high detail. We observed round silver nanoparticles with a well-defined diameter of 7.0 ± 1.5 nm that are faceted with crystalline cubic structures and ˜80% of the particles have multiply twinned faults. We also applied cryo-electron tomography to investigate the structure of the nanoparticles and to directly visualize the IL wetting around them. In addition to particles, we observed nanorods that appear to have assembled from individual nanoparticles. Reexamination of the samples after 4–5 days from initial preparation showed significant changes in morphology, and potential mechanisms for this are discussed.

scholarly journals Automated cryo-lamella preparation for high-throughput in-situ structural biology

2019 ◽  
Author(s):  
Genevieve Buckley ◽  
Gediminas Gervinskas ◽  
Cyntia Taveneau ◽  
Hari Venugopal ◽  
James C. Whisstock ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Structural Biology ◽  
Focused Ion Beam ◽  
Electron Tomography ◽  
Ion Beam ◽  
Automated Method ◽  
Cellular Environment ◽  
Transmission Electron

AbstractCryo-transmission electron tomography (cryo-ET) in association with cryo-focused ion beam (cryo-FIB) milling enables structural biology studies to be performed directly within the cellular environment. Cryo-preserved cells are milled and a lamella with a thickness of 200-300 nm provides an electron transparent window suitable for cryo-ET imaging. Cryo-FIB milling is an effective method, but it is a tedious and time-consuming process, which typically results in ~10 lamellae per day. Here, we introduce an automated method to reproducibly prepare cryo-lamellae on a grid and reduce the amount of human supervision. We tested the routine on cryo-preserved Saccharomyces cerevisiae and demonstrate that this method allows an increased throughput, achieving a rate of 5 lamellae/hour without the need to supervise the FIB milling. We demonstrate that the quality of the lamellae is consistent throughout the preparation and their compatibility with cryo-ET analyses.

Modification of clays with styrenic ionic liquid by Synthesis of in-situ Polystyrene Nanocomposite

e-Polymers ◽  
2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Mohammad Galehassadi ◽  
Fatemeh Hosseinzadeh ◽  
Mehrdad Mahkam
Keyword(s):  
Electron Microscopy ◽  
Ionic Liquid ◽  
Clay Nanocomposites ◽  
X Ray Diffraction ◽  
X Ray ◽  
Modified Montmorillonite ◽  
Transmission Electron ◽  
Insitu Polymerization ◽  
Thermal Stability Of

Abstract Nanocomposites of polystyrene (PS) was prepared with new styrenic ionic liquid, N-(4-vinyl benzyl)-(N,N-dimethylamino) pyridinium chloride[VBMAP], surfactants used as organic modifications for the clays. Sodium montmorillonite (Na-MMT) was successfully modified by [VBMAP] to become OMMT through cation exchange technique which is shown by the increase of basalspacing of clay by XRD. The composite material based on polystyrene and organo-modified montmorillonite (OMMT) was prepared by insitu polymerization and characterized. The morphology of the polymer/clay hybrids was evaluated by X-ray diffraction (XRD) ,transmission electron microscopy (TEM) and scanning electron microscopy (SEM), showing good overall dispersion of the clay. The thermal stability of the polymer/clay nanocomposites were enhanced, as evaluated by thermogravimetric analysis.

scholarly journals One-pot synthesis of polyaniline/Fe3O4 nanocomposite in ionicliquid: electrical conductivity and magnetic studies

2019 ◽  
Vol 118 ◽  
pp. 01059
Author(s):  
Xiaoyang Li ◽  
Genwang Zhang ◽  
Binwei Wang ◽  
Tan Lin ◽  
Guo Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Oxidative Polymerization ◽  
X Ray Diffraction ◽  
Magnetic Studies ◽  
Imidazolium Chloride ◽  
One Pot ◽  
One Pot Synthesis ◽  
Transmission Electron ◽  
First Time

One-pot synthesis of polyaniline/Fe3O4 nanocomposite in 1-methyl-3-alkylcarboxylic acid imidazolium chloride ([CMMIm]Cl) ionic liquid (IL) was introduced for the first time in this work. Transmission electron microscopy (TEM), X-ray diffraction (XRD), four probes method and vibrating sample magnetometer (VSM) were used to explore the influence of IL on the structure, conductivity and magnetic properties of polyaniline/Fe3O4 composite. Compared with Fe3O4 particles prepared in water, the results show that Fe3O4 particles prepare in imidazolium-based ionic liquid were more regular in shape and dispersed uniformly. So the Fe3O4 nanoparticles prepared in IL can easier serve as cores to form the polyaniline/Fe3O4 nanocomposite via in situ chemical oxidative polymerization of aniline molecule. The saturation magnetization of polyaniline/Fe3O4 nanocomposite prepared in ionic liquid shows about 2 times higher than polyaniline/Fe3O4 composite prepared in water. And the conductivities of PANI/Fe3O4 composite prepared in IL decreased and the saturated magnetization increased with the increasing amount of Fe3O4.

scholarly journals Controlling the Amorphous and Crystalline State of Multinary Alloy Nanoparticles in An Ionic Liquid

Nanomaterials ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 903 ◽  
Author(s):  
Alba Garzón-Manjón ◽  
Hajo Meyer ◽  
Dario Grochla ◽  
Tobias Löffler ◽  
Wolfgang Schuhmann ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Electron Beam ◽  
Crystalline State ◽  
Ex Situ ◽  
Alloy Nanoparticles ◽  
Face Centered Cubic ◽  
Transmission Electron ◽  
Cubic Structure ◽  
Face Centered

Controlling the amorphous or crystalline state of multinary Cr-Mn-Fe-Co-Ni alloy nanoparticles with sizes in the range between ~1.7 nm and ~4.8 nm is achieved using three processing routes. Direct current sputtering from an alloy target in the ionic liquid 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide leads to amorphous nanoparticles as observed by high-resolution transmission electron microscopy. Crystalline nanoparticles can be achieved in situ in a transmission electron microscope by exposure to an electron beam, ex situ by heating in vacuum, or directly during synthesis by using a high-power impulse magnetron sputtering process. Growth of the nanoparticles with respect to the amorphous particles was observed. Furthermore, the crystal structure can be manipulated by the processing conditions. For example, a body-centered cubic structure is formed during in situ electron beam crystallization while longer ex situ annealing induces a face-centered cubic structure.

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