Review of: Bone marrow stromal cell-derived growth inhibitor inhibits growth and migration of breast cancer cells via induction of cell cycle arrest and apoptosis

Background: Cepharanthine (CEP) is a biscoclaurine alkaloid extracted from Stephania cepharantha and has been shown to have an anti-tumour effect on different types of cancers. However, the anti-cancer effect of CEP on human breast cancer cells is still unclear. Methods: We used MTT, clone formation, in vitro scratch, invasion and migration assays to confirm the inhibitory role of CEP on the proliferation of breast cancer cells. Flow cytometry, plasmid construction and western blot analysis were used to study the detailed mechanisms. Results: Our study showed that CEP could inhibit cell proliferation by inducing autophagy, apoptosis, and G0/G1 cell cycle arrest of breast cancer cells. Furthermore, we found that CEP induced autophagy and apoptosis by inhibiting the AKT/mTOR signalling pathway. Conclusion: We found that CEP could inhibit growth and motility of MCF-7 and MDA-MB-231 breast cancer cell. Our study revealed an anti-tumour effect of CEP on breast cancer cells and suggests that CEP could be a potential new clinical therapy for breast cancer.

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Expression of potassium channels is relevant for cell survival and migration in a murine bone marrow stromal cell line

Journal of Cellular Physiology ◽

10.1002/jcp.28441 ◽

2019 ◽

Vol 234 (10) ◽

pp. 18086-18097

Author(s):

Henrique B. Silva ◽

Deivid C. Rodrigues ◽

Raiana Andrade ◽

Gabriel H. G. S. F. Teixeira ◽

Mariana P. Stelling ◽

...

Keyword(s):

Bone Marrow ◽

Potassium Channels ◽

Cell Survival ◽

Cell Line ◽

Stromal Cell ◽

Bone Marrow Stromal Cell ◽

Marrow Stromal Cell ◽

Murine Bone Marrow ◽

Stromal Cell Line ◽

And Migration

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Piperlongumine Induces Cell Cycle Arrest via Reactive Oxygen Species Accumulation and IKKβ Suppression in Human Breast Cancer Cells

Antioxidants ◽

10.3390/antiox8110553 ◽

2019 ◽

Vol 8 (11) ◽

pp. 553 ◽

Cited By ~ 7

Author(s):

Chang Hee Jeong ◽

Haram Ryu ◽

Do Hyun Kim ◽

Wei Nee Cheng ◽

Jee Eun Yoon ◽

...

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Mrna Levels ◽

Cycle Arrest ◽

Anticancer Effects ◽

Er Positive ◽

And Migration ◽

Positive Breast Cancer

Piperlongumine (PL), a natural product derived from long pepper (Piper longum L.), is known to exhibit anticancer effects. However, the effect of PL on cell cycle-regulatory proteins in estrogen receptor (ER)-positive breast cancer cells is unclear. Therefore, we investigated whether PL can modulate the growth of ER-positive breast cancer cell line, MCF-7. We found that PL decreased MCF-7 cell proliferation and migration. Flow cytometric analysis demonstrated that PL induced G2/M phase cell cycle arrest. Moreover, PL significantly modulated the mRNA levels of cyclins B1 and D1, cyclin-dependent kinases 1, 4, and 6, and proliferating cell nuclear antigen. PL induced intracellular reactive oxygen species (hydrogen peroxide) accumulation and glutathione depletion. PL-mediated inhibition of IKKβ expression decreased nuclear translocation of NF-κB p65. Furthermore, PL significantly increased p21 mRNA levels. In conclusion, our data suggest that PL exerts anticancer effects in ER-positive breast cancer cells by inhibiting cell proliferation and migration via ROS accumulation and IKKβ suppression.

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