Fluorescence Microscopy Light Sources

2012 ◽  
Vol 20 (4) ◽  
pp. 22-28 ◽  
Author(s):  
Kavita Aswani ◽  
Tushare Jinadasa ◽  
Claire M. Brown
Keyword(s):  
Fluorescence Microscopy ◽  
Visible Light ◽  
White Light ◽  
Fluorescent Proteins ◽  
Life Sciences ◽  
Light Sources ◽  
Light Spectrum ◽  
Physical Sciences ◽  
Light Emitting ◽  
Microscopy Techniques

Fluorescence microscopy techniques are now prevalent throughout the life sciences and many of the physical sciences. These techniques are often dependent on white light sources that have evolved from the more traditional mercury arc lamp to metal halide sources to the more recent light emitting diodes (LEDs). The newer light sources show more uniform power across the visible light spectrum, allowing for the use of fluorophores and fluorescent proteins outside of the peak wavelengths associated with the more traditional light sources.

scholarly journals System efficiency of laser-based white light

2014 ◽  
Vol 3 (5-6) ◽  
Author(s):  
Roland Lachmayer ◽  
Alexander Wolf ◽  
Gerolf Kloppenburg
Keyword(s):  
Thermal Management ◽  
White Light ◽  
High Power ◽  
Laser Diodes ◽  
Light Sources ◽  
System Efficiency ◽  
Light Emitting ◽  
Scale Down ◽  
Color Rendering ◽  
Product Weight

AbstractFor many lighting applications, light-emitting diodes (LEDs) are replacing traditional light sources providing the possibility for smart and efficient systems as well as a reduction in the product weight. A next step in this development is the integration of laser-based light sources to increase luminance and to further scale down the optics possibly leading to a reduction of necessary resources. This article reviews the possibilities and challenges arising from the use of laser diodes especially compared to current high-power LED systems in terms of efficiency, color-rendering properties, and thermal management.

scholarly journals White LEDs: Ultra‐Efficient and High‐Quality White Light‐Emitting Devices using Fluorescent Proteins in Aqueous Medium (Adv. Mater. Technol. 6/2020)

2020 ◽  
Vol 5 (6) ◽  
pp. 2070035
Author(s):  
Sadra Sadeghi ◽  
Rustamzhon Melikov ◽  
Deniz Conkar ◽  
Elif Nur Firat‐Karalar ◽  
Sedat Nizamoglu
Keyword(s):  
Aqueous Medium ◽  
White Light ◽  
Fluorescent Proteins ◽  
High Quality ◽  
Light Emitting ◽  
White Leds ◽  
Light Emitting Devices

scholarly journals Optimization of Multiband White-Light Illuminants for Specified Color Temperatures

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Snjezana Soltic ◽  
Andrew Neil Chalmers
Keyword(s):  
Genetic Algorithm ◽  
Differential Evolution ◽  
White Light ◽  
Color Difference ◽  
Light Sources ◽  
Optimal Solutions ◽  
Light Spectrum ◽  
Light Spectra ◽  
Wide Acceptance ◽  
Color Rendering

This paper describes an effective approach for the optimization of multiband spectra to produce prospective white-light spectra having specific color temperatures. The optimization process employs a genetic algorithm known as differential evolution, which aims to minimize the color rendering differences between a prospective white-light spectrum and its corresponding reference illuminant. Color rendering is assessed by calculating the CIEDE2000 color difference (ΔE00) for 14 CIE test colors under the two sources. Optimized white-light spectra were matched to three CIE standard illuminants, that is, A (2856 K), D50 (5003 K), and D65 (6504 K). Optimal solutions for three- and four-band 25 and 50 nm Gaussian spectra are presented and analyzed, together with mixed 4-LED spectra that were optimized in the same way. In all cases, the simulated sources were shown to provide color rendering of such quality that ΔE00av ≤ 2.24 units. Such white-light sources would likely find wide acceptance in numerous lighting applications.

Some Factors Affecting the Catches of Lepidoptera in Light Traps

1963 ◽  
Vol 95 (8) ◽  
pp. 832-837 ◽  
Author(s):  
P. Belton ◽  
R. H. Kempster
Keyword(s):  
Visible Light ◽  
White Light ◽  
Mercury Vapour ◽  
Light Sources ◽  
Light Traps ◽  
Spectral Content ◽  
Factors Affecting

AbstractBy comparing the number of moths caught in box-type traps, attractant light sources can be arranged in the following order of efficiency: 15-watt fluorescent rubes emitting ultraviolet and visible light > 15-watt fluorescent tubes emitting cool white light ≥ germicidal rubes behind glass > 15-watt fluorescent tubes emitting ultraviolet but little visible light ≥ 100-watt mercury-vapour bulbs emitting ultraviolet but little visible light. Noctuoid and large geometroid (sent. lat.) moths discriminate between these light sources on the basis of their spectral content, but pyraloids, other small microlepidoptera and some geometroids probably do not. Pyraloid moths escaped in significant numbers from traps with normal apertures (13/4-inch), after sunrise. They freely entered traps with narrow apertures (1/2-inch) but these traps caught fewer large moths.

The Color Mixing White-Light LEDs

2013 ◽  
Vol 378 ◽  
pp. 440-443
Author(s):  
Chiu Jung Yang ◽  
Chien Sheng Huang ◽  
Chih Wei Chen ◽  
Po Wen Chen
Keyword(s):  
White Light ◽  
Light Emitting Diode ◽  
Optical Power ◽  
Integrating Sphere ◽  
Light Spectrum ◽  
Light Emitting ◽  
Driver Circuit ◽  
Color Mixing ◽  
Color Rendering

Thepaperis discussedin coloruniformity study.The experiment divided into two steps in this study,first is modules design and simulation. Second is fabrication and measurement.After measure the LEDs property, calculating the ratio of each colored LEDs by using Grassmanns Law,modeling by Solidworks, and simulating the front study by optical software TracePro.Using four-color mixing with self-developed formula to avoid the present white light emitting diode patent, and the four-color grains are Red, Green, Blue and adding Y to modify the overall quality of the mixed light.The phosphorproduceSteabler-Wronsk hardly in the high temperatureas compared tofour-color mixing.Using four-color mixing to producehigher color rendering index than yellow phosphor.Series-parallel array of grain arrangement adopted to achieve the high demand for uniformity, while simplifying the design conditions by a certain current instead of the general mixed light-driven complex driver circuit,the completion of the mixing module using integrating sphere, light spectrum on the spectrophotometer, optical power, color coordinates values, such as mixing uniformity measurements.The chromaticity coordinates errors after complete results of the mixing module measurement and simulation can be controlled under (0.01x, 0.01y).

Portable visible light communication transmitter and receiver using core-shell CdSe/ZnS quantum dots white light-emitting diode

2019 ◽  
Vol 13 (7) ◽  
pp. 873-878
Author(s):  
Ziwen Li ◽  
Dingke Xue ◽  
Dayong Liu ◽  
Yang Liu ◽  
Weilin Ye ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Visible Light ◽  
White Light ◽  
Light Emitting Diode ◽  
Visible Light Communication ◽  
Core Shell ◽  
Light Emitting
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