Fluorescence Microscopy Light Sources

2012 ◽  
Vol 20 (4) ◽  
pp. 22-28 ◽  
Author(s):  
Kavita Aswani ◽  
Tushare Jinadasa ◽  
Claire M. Brown

Fluorescence microscopy techniques are now prevalent throughout the life sciences and many of the physical sciences. These techniques are often dependent on white light sources that have evolved from the more traditional mercury arc lamp to metal halide sources to the more recent light emitting diodes (LEDs). The newer light sources show more uniform power across the visible light spectrum, allowing for the use of fluorophores and fluorescent proteins outside of the peak wavelengths associated with the more traditional light sources.

2014 ◽  
Vol 3 (5-6) ◽  
Author(s):  
Roland Lachmayer ◽  
Alexander Wolf ◽  
Gerolf Kloppenburg

AbstractFor many lighting applications, light-emitting diodes (LEDs) are replacing traditional light sources providing the possibility for smart and efficient systems as well as a reduction in the product weight. A next step in this development is the integration of laser-based light sources to increase luminance and to further scale down the optics possibly leading to a reduction of necessary resources. This article reviews the possibilities and challenges arising from the use of laser diodes especially compared to current high-power LED systems in terms of efficiency, color-rendering properties, and thermal management.


2020 ◽  
Vol 5 (6) ◽  
pp. 2070035
Author(s):  
Sadra Sadeghi ◽  
Rustamzhon Melikov ◽  
Deniz Conkar ◽  
Elif Nur Firat‐Karalar ◽  
Sedat Nizamoglu

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Snjezana Soltic ◽  
Andrew Neil Chalmers

This paper describes an effective approach for the optimization of multiband spectra to produce prospective white-light spectra having specific color temperatures. The optimization process employs a genetic algorithm known as differential evolution, which aims to minimize the color rendering differences between a prospective white-light spectrum and its corresponding reference illuminant. Color rendering is assessed by calculating the CIEDE2000 color difference (ΔE00) for 14 CIE test colors under the two sources. Optimized white-light spectra were matched to three CIE standard illuminants, that is, A (2856 K), D50 (5003 K), and D65 (6504 K). Optimal solutions for three- and four-band 25 and 50 nm Gaussian spectra are presented and analyzed, together with mixed 4-LED spectra that were optimized in the same way. In all cases, the simulated sources were shown to provide color rendering of such quality that ΔE00av ≤ 2.24 units. Such white-light sources would likely find wide acceptance in numerous lighting applications.


1963 ◽  
Vol 95 (8) ◽  
pp. 832-837 ◽  
Author(s):  
P. Belton ◽  
R. H. Kempster

AbstractBy comparing the number of moths caught in box-type traps, attractant light sources can be arranged in the following order of efficiency: 15-watt fluorescent rubes emitting ultraviolet and visible light > 15-watt fluorescent tubes emitting cool white light ≥ germicidal rubes behind glass > 15-watt fluorescent tubes emitting ultraviolet but little visible light ≥ 100-watt mercury-vapour bulbs emitting ultraviolet but little visible light. Noctuoid and large geometroid (sent. lat.) moths discriminate between these light sources on the basis of their spectral content, but pyraloids, other small microlepidoptera and some geometroids probably do not. Pyraloid moths escaped in significant numbers from traps with normal apertures (13/4-inch), after sunrise. They freely entered traps with narrow apertures (1/2-inch) but these traps caught fewer large moths.


2013 ◽  
Vol 378 ◽  
pp. 440-443
Author(s):  
Chiu Jung Yang ◽  
Chien Sheng Huang ◽  
Chih Wei Chen ◽  
Po Wen Chen

Thepaperis discussedin coloruniformity study.The experiment divided into two steps in this study,first is modules design and simulation. Second is fabrication and measurement.After measure the LEDs property, calculating the ratio of each colored LEDs by using Grassmanns Law,modeling by Solidworks, and simulating the front study by optical software TracePro.Using four-color mixing with self-developed formula to avoid the present white light emitting diode patent, and the four-color grains are Red, Green, Blue and adding Y to modify the overall quality of the mixed light.The phosphorproduceSteabler-Wronsk hardly in the high temperatureas compared tofour-color mixing.Using four-color mixing to producehigher color rendering index than yellow phosphor.Series-parallel array of grain arrangement adopted to achieve the high demand for uniformity, while simplifying the design conditions by a certain current instead of the general mixed light-driven complex driver circuit,the completion of the mixing module using integrating sphere, light spectrum on the spectrophotometer, optical power, color coordinates values, such as mixing uniformity measurements.The chromaticity coordinates errors after complete results of the mixing module measurement and simulation can be controlled under (0.01x, 0.01y).


2019 ◽  
Vol 13 (7) ◽  
pp. 873-878
Author(s):  
Ziwen Li ◽  
Dingke Xue ◽  
Dayong Liu ◽  
Yang Liu ◽  
Weilin Ye ◽  
...  

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