Suitability of a liquid larval diet for rearing the Philippines fruit fly Bactrocera philippinensis (Diptera: Tephritidae)

ABSTRACTAdult body size is determined by the quality and quantity of nutrients available to animals. In insects, nutrition affects adult size primarily during the nymphal or larval stages. However, measures of adult size like body weight are likely to also change with adult nutrition. In this study, we sought to the roles of nutrition throughout the life cycle on adult body weight and the size of two appendages, the wing and the femur, in the fruit fly Drosophila melanogaster. We manipulated nutrition in two ways: by varying the protein to carbohydrate content of the diet, called macronutrient restriction, and by changing the caloric density of the diet, termed caloric restriction. We employed a fully factorial design to manipulate both the larval and adult diets for both diet types. We found that manipulating the larval diet had greater impacts on all measures of adult size. Further, macronutrient restriction was more detrimental to adult size than caloric restriction. For adult body weight, a rich adult diet mitigated the negative effects of poor larval nutrition for both types of diets. In contrast, small wing and femur size caused by poor larval diet could not be increased with the adult diet. Taken together, these results suggest that appendage size is fixed by the larval diet, while those related to body composition remain sensitive to adult diet. Further, our studies provide a foundation for understanding how the nutritional environment of juveniles affects how adults respond to diet.

Download Full-text

Effects of larval‐diet pH on worker comfort and insect quality during mass production of mediterranean fruit fly (Diptera: Tephritidae) in Hawaii

Journal of Environmental Science and Health Part A Environmental Science and Engineering ◽

10.1080/10934528409375181 ◽

1984 ◽

Vol 19 (5) ◽

pp. 621-630 ◽

Cited By ~ 2

Author(s):

Roger I. Vargas ◽

D. Leroy Williamson ◽

Harris Chang ◽

Masami Komura

Keyword(s):

Mass Production ◽

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Larval Diet ◽

Insect Quality

Download Full-text

Age, sex, adult and larval diet shape starvation resistance in the Mediterranean fruit fly: an ecological and gerontological perspective

Scientific Reports ◽

10.1038/s41598-019-47010-0 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Christos D. Gerofotis ◽

Nikos A. Kouloussis ◽

Christiana Koukougiannidou ◽

Nikos T. Papadopoulos ◽

Petros Damos ◽

...

Keyword(s):

Fruit Fly ◽

Mediterranean Fruit Fly ◽

Larval Diet ◽

Starvation Resistance ◽

The Mediterranean

Download Full-text

Enterobacter sp. AA26 gut symbiont as a protein source for Mediterranean fruit fly mass-rearing and sterile insect technique applications

BMC Microbiology ◽

10.1186/s12866-019-1651-z ◽

2019 ◽

Vol 19 (S1) ◽

Cited By ~ 7

Author(s):

Georgios A. Kyritsis ◽

Antonios A. Augustinos ◽

Spyridon Ntougias ◽

Nikos T. Papadopoulos ◽

Kostas Bourtzis ◽

...

Keyword(s):

Sterile Insect Technique ◽

Fruit Fly ◽

Mass Rearing ◽

Mediterranean Fruit Fly ◽

Protein Source ◽

Pupal Weight ◽

Larval Diet ◽

Brewer’S Yeast ◽

Complete Replacement ◽

Brewer's Yeast

Abstract Background Insect species have established sophisticated symbiotic associations with diverse groups of microorganisms including bacteria which have been shown to affect several aspects of their biology, physiology, ecology and evolution. In addition, recent studies have shown that insect symbionts, including those localized in the gastrointestinal tract, can be exploited for the enhancement of sterile insect technique (SIT) applications against major insect pests such as the Mediterranean fruit fly (medfly) Ceratitis capitata. We previously showed that Enterobacter sp. AA26 can be used as probiotic supplement in medfly larval diet improving the productivity and accelerating the development of the VIENNA 8 genetic sexing strain (GSS), which is currently used in large scale operational SIT programs worldwide. Results Enterobacter sp. AA26 was an adequate nutritional source for C. capitata larvae, comprising an effective substitute for brewer’s yeast. Incorporating inactive bacterial cells in the larval diet conferred a number of substantial beneficial effects on medfly biology. The consumption of bacteria-based diet (either as full or partial yeast replacement) resulted in decreased immature stages mortality, accelerated immature development, increased pupal weight, and elongated the survival under stress conditions. Moreover, neither the partial nor the complete replacement of yeast with Enterobacter sp. AA26 had significant impact on adult sex ratio, females’ fecundity, adults’ flight ability and males’ mating competitiveness. The absence of both yeast and Enterobacter sp. AA26 (deprivation of protein source and possible other important nutrients) from the larval diet detrimentally affected the larval development, survival and elongated the immature developmental duration. Conclusions Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS as assessed by the FAO/IAEA/USDA standard quality control tests. We discuss this finding in the context of mass-rearing and SIT applications.

Download Full-text

LABORATORY REARING OF THE WESTERN CHERRY FRUIT FLY, RHAGOLETIS INDIFFERENS (DIPTERA: TEPHRITIDAE): OVIPOSITION AND LARVAL DIETS

The Canadian Entomologist ◽

10.4039/ent1091227-9 ◽

1977 ◽

Vol 109 (9) ◽

pp. 1227-1234 ◽

Cited By ~ 9

Author(s):

M. T. AliNiazee ◽

R. D. Brown

Keyword(s):

Wheat Germ ◽

Artificial Diet ◽

Choline Chloride ◽

Fruit Fly ◽

The Other ◽

Larval Diet ◽

Tropical Fruit ◽

Laboratory Rearing ◽

Greenish Yellow ◽

Rhagoletis Indifferens

AbstractIn a laboratory study, larvae of the western cherry fruit fly, Rhagoletis indifferens Curran, were successfully reared on an artificial diet consisting of wood pulp, agar, wheat germ, Brewer’s yeast, sugar, salt, choline chloride, ascorbic, citric and propionic acids, cholesterol, methyl para-hydroxybenzoate (MPH), and water. Ten other diets consisting of various ingredients obtained variable results. A premixed tropical fruit fly diet (prepared by Nutritional Biochemicals, Cleveland, Ohio) was not suitable for rearing the cherry maggots. Formaldehyde was not satisfactory as a mold inhibitor.The eggs were obtained on artificial cherries made up of a soft ceresin wax. No oviposition occurred on the other materials tested (candle wax, a regular ceresin wax, beeswax, and silicone). Significantly (5% level) more eggs were deposited on wax cherries of orange, green, and greenish-yellow colors than black, red, white and blue. A laboratory rearing method employing orange colored wax cherries and an artificial larval diet was established for culturing the western cherry fruit flies.

Download Full-text

APLIKASI MAT DALAM PENGENDALIAN LALAT BUAH Bactrocera sp. (DIPTERA : TEPHRITIDAE) PADA TANAMAN CABE

EUGENIA ◽

10.35791/eug.17.2.2011.3533 ◽

2011 ◽

Vol 17 (2) ◽

Author(s):

Maxi Lengkong ◽

Caroulus S. Rante ◽

Merlyn Meray

Keyword(s):

Field Research ◽

Fruit Fly ◽

Fruit Flies ◽

Methyl Eugenol ◽

Bactrocera Dorsalis ◽

Crop Acreage ◽

Bactrocera Carambolae ◽

Male Annihilation ◽

Bactrocera Philippinensis ◽

Male Annihilation Technique

ABSTRACT Research was aiming to determine the effectiveness of the attractant methyl eugenol + traps modification in the catch of species of fruit flies in the chilli plants at any given time and to identify the types of fruit flies trapped with methyl eugenol + traps modification on chilli crop acreage. The field research was conducted in the area of chilli crop in three places namely District Pineleng, Tompaso / Langowan and Modoinding. Observation number and type of fruit flies was performed at intervals of 2 weeks of observation for 3 months. The result showed that the number of fruit fly caught or trapped dead at 30 traps set in chilli crop acreage was 1278. The highest trapped was 164 whereas the lowest trapped was 89. The number of male fruit flies trapped was high implying that reduction of male fruit fly population in the field. There were five types of fruit flies identified from area of Pineleng, Tompaso and Modoinding namely Bactrocera umbrosa Fabricus, Bactrocera dorsalis (Hendel), Bactrocera philippinensis, Bactrocera carambolae, and Bactrocera sp. The five types were attracted to methyl eugenol. Key words : MAT (male annihilation technique), Bactrocera umbrosa Fabricus, B. dorsalis (Hendel), B. philippinensis, B. carambolae

Download Full-text