Factors affecting bioleaching in the processing of non-metallics

Biotechnological treatment of non-metallics is based on bacterial leaching of raw material and dissolution of Fe. Bacterial iron dissolution ability is dependent on various physicochemical factors as temperature, acidity of solutions, redox potential, rapidity of water circulation and presence of organic sources. The Fe content in the quartz sands and feldspar samples by the biological leaching decreased as much as 60% and by subsequent using of electromagnetic separation of feldspars, the decrease of Fe content in 74% was achieved. However, the application of magnetic separation of quartz sands after bioleaching resulted in total iron removal of 93% and in such combined way prepared product contained 0.024 % of Fe2O3. Achieved results on iron removal point to the fact that combination of leaching and magnetic separation enables to obtain product usable in glass and ceramic industry.

Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress

Background Chilling temperature reduces the rate of photosynthesis in plants, which is more pronounced in association with phosphate (Pi) starvation. Previous studies showed that Pi resupply improves recovery of the rate of photosynthesis in plants much better under combination of dual stresses than in non-chilled samples. However, the underlying mechanism remains poorly understood. Results In this study, RNA-seq analysis showed the expression level of 41 photosynthetic genes in plant roots increased under phosphate starvation associated with 4 °C (-P 4 °C) compared to -P 23 °C. Moreover, iron uptake increased significantly in the stem cell niche (SCN) of wild type (WT) roots in -P 4 °C. In contrast, lower iron concentrations were found in SCN of aluminum activated malate transporter 1 (almt1) and its transcription factor, sensitive to protein rhizotoxicity 1 (stop1) mutants under -P 4 °C. The Fe content examined by ICP-MS analysis in -P 4 °C treated almt1 was 98.5 ng/µg, which was only 17% of that of seedlings grown under -P 23 °C. Average plastid number in almt1 root cells under -P 4 °C was less than -P 23 °C. Furthermore, stop1 and almt1 single mutants both exhibited increased primary root elongation than WT under combined stresses. In addition, dark treatment blocked the root elongation phenotype of stop1 and almt1. Conclusions Induction of photosynthetic gene expression and increased iron accumulation in roots is required for plant adjustment to chilling in association with phosphate starvation.

An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi

The parasite Trypanosoma cruzi causes Chagas’ disease; both heme and ionic Fe are required for its optimal growth, differentiation, and invasion. Fe is an essential cofactor in many metabolic pathways. Fe is also harmful due to catalyzing the formation of reactive O2 species; for this reason, all living systems develop mechanisms to control the uptake, metabolism, and storage of Fe. However, there is limited information available on Fe uptake by T. cruzi. Here, we identified a putative 39-kDa Fe transporter in T. cruzi genome, TcIT, homologous to the Fe transporter in Leishmania amazonensis and Arabidopsis thaliana. Epimastigotes grown in Fe-depleted medium have increased TcIT transcription compared with controls grown in regular medium. Intracellular Fe concentration in cells maintained in Fe-depleted medium is lower than in controls, and there is a lower O2 consumption. Epimastigotes overexpressing TcIT, which was encountered in the parasite plasma membrane, have high intracellular Fe content, high O2 consumption—especially in phosphorylating conditions, high intracellular ATP, very high H2O2 production, and stimulated transition to trypomastigotes. The investigation of the mechanisms of Fe transport at the cellular and molecular levels will assist in elucidating Fe metabolism in T. cruzi and the involvement of its transport in the differentiation from epimastigotes to trypomastigotes, virulence, and maintenance/progression of the infection.

Effects of Te- and Fe-doping on the superconducting properties in FeySe1−xTex thin films

High quality FeySe1−xTex epitaxial thin films have been fabricated on TiO2-buffered SrTiO3 substrates by pulsed laser deposition technology. There is a significant composition deviation between the nominal target and the thin film. Te doping can affect the Se/Te ratio and Fe content in chemical composition. The superconducting transition temperature Tc is closely related to the chemical composition. Fe vacancies are beneficial for the FeySe1−xTex films to exhibit the higher Tc. A 3D phase diagram is given that the optimize range is x = 0.13–0.15 and y = 0.73–0.78 for FeySe1−xTex films. The anisotropic, effective pining energy, and critical current density for the Fe0.72Se0.94Te0.06, Fe0.76Se0.87Te0.13 and Fe0.91Se0.77Te0.23 films were studied in detail. The scanning transmission electron microscopy images display a regular atomic arrangement at the interfacial structure.

Assessment of contamination level of a Tanzanian river system with respect to trace metallic elements and their fate in the environment

The quality of water and sediments from a marginally-studied river was investigated with respect to As, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn including their fractionation behavior and environmental risk. Samples were collected along the Kou River that flows across two districts in the Manyara region of Tanzania. The leaching behavior of Fe was studied using sequential extraction fractionation and kinetics approach. The Kou water failed to meet the irrigation, aquatic, and biological life standards with respect to one of more trace metallic elements (TMEs). Fe concentration in the river water ranged from 4.1 to 5.38 mg/L exceeding all the three standards. Six pollution indices were applied to assess the contamination and ecological risks of the nine trace metallic elements in the sediments. Overall, the metals were found to moderately contaminate the sediments. Cr, Fe, and Mn fell under the ‘severely polluted’ sediment quality class. Fe was the only metal that was found to significantly pollute both the river water and sediments. The Fe fractions in the sediments were in the order of residuals>Fe-Mn bound>organic bound>carbonate bound>water soluble>ion exchangeable. 7.8% of the total Fe content was bioavailable with a low potential to leach from the sediments. Under natural conditions, the sharpest release of the non-residual mobile fractions of Fe were identified to occur within the first 24 hours with the maximum Fe leached being 0.14% on the 12th day. None of the metals in the sediments were found with a potential to pose ecological risk.

Determination of heavy metal elements concentration in soils and tailing sediments from lateritic nickel post-mining areas in Motui District, Southeast Sulawesi

Heavy metal elements concentration study has been determined from soils and tailing sediments in laterite nickel post-mining area in Motui District Southeast Sulawesi. This study aimed to determine the concentration of some heavy metal elements, especially Fe, Co, Mn and Cr, from surface soils sediments in waste dump sites and tailing sediments in settling ponds from lateritic nickel post-mining areas. A total of 20 samples consisting of 18 soil samples and 2 tailing sediments samples were systematically collected for the study. The soil samples from the waste dump site profile were collected from 3 layers which were divided based on the colour of the soils from top to bottom, namely Layer C, Layer D and Layer E. Six soil samples were taken from each layer with space between each sample in one layer was about 50 – 60 cm. The samples were sent to the laboratory and analysed using Atomic Absorption Spectrometer (AAS) method to determine the concentration of heavy elements. Metal-bearing minerals detected from the bedrock consists of chromite, manganese, magnetite and limonite which are responsible for the Cr, Mn and Co, and Fe content, respectively. The result showed that Fe content is significantly higher in soil samples from Layer C and tailing sediments with dark red to brown in colour, suggesting the strong relation between Fe content and colour index. The general element mobility trend showed that Mn and Co are positively correlated in soil sampling from all layers and tailing samples, whereas Fe and Cr show a negative correlation trend in Layer C, D and tailing sediments but positively correlated in Layer E.

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Some Bacterial Soft Rot/Blackleg Genera ex vivo and Bio-Control in vivo as Eco-Friendly Therapeutic against Dickeya Solani

The antibacterial activity of Cu, Fe, Co, and ZnNPs which were formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate sequentially was tested against some soft rot/blackleg genera. The effects of NPs were recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in the DNA isolated, and a decrease in proteins, and carbohydrates concentration compared with untreated cells. The treated cells using SEM showed collapsed, and small pits in the cell wall. Internal changes using TEM showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation vacuoles, and condensation of cytoplasm. Disease severity ex vivo of tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. FeNPs were tested to control soft rot/blackleg disease caused by D. solani in comparison with copper pesticide. Present data recorded an increase in shoot and root length, in addition to, increase in dry and fresh weight, compared with either infected or healthy plants. In studying the ability of treated potato (Solanum tuberosum) seedlings with NPs to uptake and accumulate FeNPs from the soil, ICP-OES recorded a small increase in Fe content of treated plants compared with untreated. FeNPs can be used to control soft rot/blackleg disease caused by D. solani instead of copper pesticide and can be considered as a new and alternative approach to traditional disease management methods, and also increase the nutritional value of the plants

A Comprehensive Updated Review on Magnetic Nanoparticles in Diagnostics

Magnetic nanoparticles (MNPs) have been studied for diagnostic purposes for decades. Their high surface-to-volume ratio, dispersibility, ability to interact with various molecules and superparamagnetic properties are at the core of what makes MNPs so promising. They have been applied in a multitude of areas in medicine, particularly Magnetic Resonance Imaging (MRI). Iron oxide nanoparticles (IONPs) are the most well-accepted based on their excellent superparamagnetic properties and low toxicity. Nevertheless, IONPs are facing many challenges that make their entry into the market difficult. To overcome these challenges, research has focused on developing MNPs with better safety profiles and enhanced magnetic properties. One particularly important strategy includes doping MNPs (particularly IONPs) with other metallic elements, such as cobalt (Co) and manganese (Mn), to reduce the iron (Fe) content released into the body resulting in the creation of multimodal nanoparticles with unique properties. Another approach includes the development of MNPs using other metals besides Fe, that possess great magnetic or other imaging properties. The future of this field seems to be the production of MNPs which can be used as multipurpose platforms that can combine different uses of MRI or different imaging techniques to design more effective and complete diagnostic tests.