Effect of water quality on in vitro fermentation of sorghum and barley for poultry diets

2007 ◽  
Vol 2007 ◽  
pp. 243-243
Author(s):  
A.T. Niba ◽  
J.D. Beal ◽  
A.C. Kudi ◽  
P.H. Brooks
Keyword(s):  
Water Quality ◽  
Lactic Acid ◽  
Acid Concentration ◽  
Lactic Acid Concentration ◽  
In Vitro Fermentation ◽  
Effect Of Water ◽  
Fermented Feed ◽  
Fermentation Pattern ◽  
Poultry Diets

Low pH and high lactic acid concentration of fermented feed has been reported to be responsible for the antimicrobial activity of fermented feeds (Brooks et al., 2001). For example, to prevent the growth of Salmonella spp. in liquid feeds, a threshold lactic acid concentration of 75mM is required (Beal et al., 2002). Therefore, factors that are likely to affect the production of lactic acid during fermentation will have important implications for the ability of such feeds to withstand colonisation by pathogens. The objective of the present study was to investigate the effect of water quality on the fermentation pattern of sorghum and barley.

scholarly journals Concentrate feeding and ruminal fermentation. 2. Influence of concentrate ingredients on pH and on L-lactate concentration in incubations in vitro with rumen fluid.

1982 ◽  
Vol 30 (4) ◽  
pp. 259-274
Author(s):  
A. Malestein ◽  
A.T. van 't Klooster ◽  
G.H.M. Counotte ◽  
R.A. Prins
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Rumen Fluid ◽  
Lactate Concentration ◽  
Lactic Acid Concentration ◽  
Ruminal Fermentation ◽  
Beet Pulp ◽  
Effect Of Particle Size ◽  
Maize Meal

2. Rumen fluid was sampled before feeding from cows given hay, diluted with an anaerobic salt solution and added (20 ml) to different amounts (mostly 1 g) of maize gluten meal, maize, citrus pulp, tapioca, beet pulp, coconut expeller or soya bean oilmeal for incubation at 39 deg C. After at least 4 h of incubation there were large differences in pH and lactic acid concentration. The acidotic index of the feeds was influenced by increasing concentration of the substrate. Except with maize meal, there was little effect of particle size on pH and lactic acid concentration. There were differences in effect on pH and lactic acid concentration between different batches of the same feeds, especially with maize meal. Incubations with mixtures of concentrate ingredients showed different pH and lactic acid concentrations from values expected from results with the single ingredients. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Concentration Dependent Influence of Lipopolysaccharides on Separation of Hoof Explants and Supernatant Lactic Acid Concentration in an Ex Vivo/In Vitro Laminitis Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143754 ◽  
Author(s):  
Nicole Reisinger ◽  
Simone Schaumberger ◽  
Veronika Nagl ◽  
Sabine Hessenberger ◽  
Gerd Schatzmayr
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Ex Vivo ◽  
Lactic Acid Concentration

Blood osmolality in vitro: dependence on PCO2, lactic acid concentration, and O2 saturation

1983 ◽  
Vol 54 (1) ◽  
pp. 118-122 ◽  
Author(s):  
D. Boning ◽  
N. Maassen
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Freezing Point ◽  
Co2 Absorption ◽  
Lactic Acid Concentration ◽  
Co2 Partial Pressure ◽  
Constant Ph ◽  
Acid Titration ◽  
Point Determination

Changes of osmolality (Osm) were measured by freezing-point determination in true plasma of 10 healthy subjects. This was done after equilibration with CO2 (0.5–10.0%), after the addition of lactic acid (10 and 20 mmol/l), and after deoxygenation. The graph for the dependence of Osm on CO2 partial pressure (PCO2) in oxygenated blood resembles the classical CO2 absorption curve. The increase of Osm with PCO2 (approximately 0.2 mosmol . kg H2O-1 . Torr-1) is almost as great as the increase in dissolved CO2 plus bicarbonate (HCO-3). Addition of lactic acid shifts the curve upward by only 0.6 mosmol/mmol because of displacement of HCO-3. Deoxygenation has no significant effect at constant PCO2 despite an increase in [HCO-3]. This is probably due to the binding of 2,3-diphosphoglycerate to hemoglobin. It can be seen in the Osm-pH diagram that differences between CO2 and lactic acid titration largely disappear. For each lactic acid concentration there is a linear dependence corresponding to the linear [HCO-3]-pH relation in plasma. At constant pH, Osm increases after deoxygenation. The observed in vitro relation might explain part of the osmolality increase during physical exercise.

scholarly journals Effect of Lactic Acid Bacteria on the Pharmacokinetics and Metabolism of Ginsenosides in Mice

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1496
Author(s):  
Ji-Hyeon Jeon ◽  
Jaehyeok Lee ◽  
Jin-Hyang Park ◽  
Chul-Haeng Lee ◽  
Min-Koo Choi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Formation Rate ◽  
Plasma Concentrations ◽  
Control Level ◽  
In Vitro Fermentation ◽  
Fecal Recovery ◽  
Fermentation Test

This study aims to investigate the effect of lactic acid bacteria (LAB) on in vitro and in vivo metabolism and the pharmacokinetics of ginsenosides in mice. When the in vitro fermentation test of RGE with LAB was carried out, protopanaxadiol (PPD) and protopanaxadiol (PPD), which are final metabolites of ginsenosides but not contained in RGE, were greatly increased. Compound K (CK), ginsenoside Rh1 (GRh1), and GRg3 also increased by about 30%. Other ginsenosides with a sugar number of more than 2 showed a gradual decrease by fermentation with LAB for 7 days, suggesting the involvement of LAB in the deglycosylation of ginsenosides. Incubation of single ginsenoside with LAB produced GRg3, CK, and PPD with the highest formation rate and GRd, GRh2, and GF with the lower rate among PPD-type ginsenosides. Among PPT-type ginsenosides, GRh1 and PPT had the highest formation rate. The amoxicillin pretreatment (20 mg/kg/day, twice a day for 3 days) resulted in a significant decrease in the fecal recovery of CK, PPD, and PPT through the blockade of deglycosylation of ginsenosides after single oral administrations of RGE (2 g/kg) in mice. The plasma concentrations of CK, PPD, and PPT were not detectable without change in GRb1, GRb2, and GRc in this group. LAB supplementation (1 billion CFU/2 g/kg/day for 1 week) after the amoxicillin treatment in mice restored the ginsenoside metabolism and the plasma concentrations of ginsenosides to the control level. In conclusion, the alterations in the gut microbiota environment could change the ginsenoside metabolism and plasma concentrations of ginsenosides. Therefore, the supplementation of LAB with oral administrations of RGE would help increase plasma concentrations of deglycosylated ginsenosides such as CK, PPD, and PPT.

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