A Substitute for Fishmeal in the Diet of Broiler Chicks

The aim of this research was to study the biochemical properties of a new protein-rich feed additive produced by the short-term intense thermal treatment and subsequent enzymatic hydrolysis of the wastes of poultry slaughter and primary processing (feathers and fluff). It was found that this feather-based fermented feed additive contained high amounts of crude protein (86.52%); and the content of easily digestible low-molecular peptides in the additive was 9% higher compared to fishmeal. The amino acid profiles of the additive and fishmeal were compared. The effectiveness of substituting the additive for fishmeal in the diet of broiler chicks was demonstrated by the in vivoexperiments. The results showed that the digestibility of the dietary nutrients was higher in broilers that were fed the new additive compared to those fed fishmeal, which resulted in higher meat productivity: the average daily weight gains in additive-fed broilers was 3.82% higher (p <0.01) compared to fishmeal-fed control broilers, the dressing was 1.4%higher, the muscle in the carcass was 2.1% higher, and the feed conversion ratio was 3.57%lower. The sensory evaluation scores of the meat and broth were also higher in the additive-fed broilers. Keywords: feedadditive, feather wastes of poultry slaughter, enzymatic hydrolysis, distribution of molecular peptide weights, digestibility, productive performance in broilers

Increase in body weight and protein retention on meat chicken due to the addition of probiotics and digestive enzymes in fermented diet containing maggot flour and local materials

The purpose of this study was to determine the growth response of meat chickens due to the addition of probiotics and digestive enzymes in the fermented diet containing maggot flour and local materials. The research was carried out at Field Laboratory of Animal Science, University of Syiah Kuala-Banda Aceh, Indonesia for 66 days. A total of 100 meat chickens were designed using a completely randomized design (CRD) consisting of 5 treatments, namely P0 = 100% fermented ration (control), P1 = 0.5% probiotic + 0% digestive enzymes + 100% fermented diet, P2 = 0% probiotic + 0.5% digestive enzymes + 100% fermented diet, P3 = 0.5% probiotic + 1% digestive enzymes + 100% fermented diet, P4 = 1% probiotic + 0.5% digestive enzymes + 100% fermented diet with 4 replications. The data obtained were analyzed using ANOVA (Analysis of variance) and significant difference of data was analyzed by Duncan’s multiple distance test. The results showed that the addition of probiotics and digestive enzymes with different levels of administration in the fermented diet had a significant effect (P<0.05) on the increase in final body weight and percentage of protein retention. The best results were obtained on chicken fed on fermented diet contained 0, 5% digestive enzymes by increasing on final body weight, protein retention, feed conversion and feed efficiency which were higher than fermented feed containing probiotics. In conclusion, it was well known that digestive enzyme effected synergistically on fermented diet in increasing protein retention resulted a higher final body weight of meat chicken.

Optimization of Small Peptide Feed from Milk Thistle Residue by Synergistic Fermentation of Multiple Strains and Proteases

In order to improve the utilization rate of the milk thistle residue, this study used the synergistic fermentation of multiple strains and proteases to increase the small peptide content of the fermented feed produced by the milk thistle residue. Taking the small peptide content of the milk thistle residue fermented feed as an indicator, the optimal fermentation process was obtained by single-factor optimization experiments and the response surface methodology. The optimal fermentation process was as follows: fermentation time of 7 days, inoculum size of 15%, inoculation ratio of aerobic strains: anaerobic strains = 1: 2, solid-state fermentation water content of 66%, fermentation temperature of 36℃, and amount of protease was 0.25% acid protease+0.25% bromelain. Under the above process, the small peptide content of the fermented feed from milk thistle residue was greatly improved to 57.86%. These results inferred that the added proteases were beneficial to the growth of fermentative microorganisms, the secretion of protease and the increase of the small peptide content.

Investigation on the changes of Bifidobacterium and lactic acid bacteria in soybean meal fermented feed

The main objective of this research was to explore the dynamic changes of Bifidobacterium and lactic acid bacteria (LAB) in the process of feed fermentation under anaerobic condition, so as to increase the number of fermented bacteria of Bifidobacterium from the aspect of strain combination. The results showed that when Bifidobacterium lactis ( B. lactis, i.e. Bifidobacterium animalis subsp. lactis ) fermented with Bacillus coagulans or Lactobacillus paracasei , the maximum number of B. lactis in those samples was 9.42 times and 4.64 times of that of fermented sample with B. lactis only. The soybean meal was fermented by B. lactis, L. paracasei and B. coagulans , and the number of B. lactis reached the maximum after fermented 10 days, which was 6.13 times of that in unfermented sample. The reducing sugar content and highest activity of α-galactosidase were higher than the control. These results suggest that B . coagulans and L . paracasei can promote the growth of B. lactis . It is inferred that B . coagulans can metabolize normally in aerobic, micro-aerobic and anaerobic environments, consume oxygen, produce digestive enzymes, and cooperate with L . paracasei to produce metabolic products benefit for the growth of B. lactis .

Effect of Fermented Liquid Feed (FLF) on Performance and Feed Efficiency of Large White Yorkshire (LWY) Pigs under Tropical Climate of North-East India

Background: Feeding of fermented feed is not popular among the pig farmers inspite of manifold advantages as preparation is laborious, involves technical knowledge requiring considerable time. An attempt was made to standardise a preparation method of FLF and comparative assessment was made with dry and liquid feed in LWY pigs. Methods: Twenty-four weaned LWY piglets (11.45±2.42 to 11.46±2.37 kg) were assigned - dry feed (T1), Liquid feed (T2), liquid feed fermented with Lactobacillus acidophilus (T3) and liquid feed fermented with Enterococcus faecium (T4) in a 180 days feeding trial. Liquid feed was prepared by mixing feed and water at 1:2 (w/w) and the FLFs were prepared by fermenting liquid feed with Lactobacillus acidophilus for T3 and Enterococcus faecium for T4. Pigs were fed individually ad libitum considering each piglet as replicate. Nutrient digestibility was estimated at 18th and 30th week of age conducting two feeding trials. Carcass traits and sensory quality of pork were evaluated by slaughtering 3 pigs from each treatment at the end of trial. Result: No significant effect (P greater than 0.05) of FLFs was observed in feed intake in the growing phase, but it was significantly high in T3 and T4 in the finishing phase. Significantly (P less than 0.05) high body weight gain with improvement of 17.76% in T3 and 17.71% in T4 were recorded. Apparent nutrient digestibility was better in T3 and T4 and crude protein digestibility was significantly (P less than 0.05) high in T3 in finishing phase. Significantly improved feed efficiency was recorded for T3 and T4. The feeding cost/kg body weight gain was Rs. 128.36, 120.43, 112.87 and 115.51, respectively for T1, T2, T3 and T4. Significantly high dressing% and carcass length with positive effect on water holding capacity were observed for feeding FLFs, but without any significant effect on proximate composition and sensory attributes of pork.

Effects of Fermented Feed on the Growth Performance, Intestinal Function and Microbiota of Piglets Weaned at Different Age

BACKGROUND: The beneficial function of fermented feed in livestock industry has been widely investigated. However, little is known about the effects of fermented feed on different weaned-day piglets. This study aimed to investigate the effects of fermented diet on the growth performance, intestinal function and microbiota of piglets weaned at age of 21 days and 28 days.RESULTS: The results found that weaning on d 21 significantly increased (P < 0.05) ADG, and ADFI (calculated based on wet weight and dry matter), while reduced (P < 0.05) F: G, the activities of trypsin and lipase of jejunum and villus height of ileum, compared with 28-d weaning. The protein levels of Occludin, Claudin-1, ZO-1 of ileum in the groups weaning on d 21 were less (P < 0.05) than the groups weaning on d 28. Moreover, dietary supplementation with fermented diet upregulated (P < 0.05) Occludin, Claudin-1, ZO-1 proteins of ileum, compared with the groups treated with control diet both weaning on d 21 and d 28. In addition, dietary supplementation with fermented diet decreased (P < 0.05) the relative abundance of Clostridia (class) and increased (P < 0.05) Bacteroidia (class) level of cecal microbiota, compared with the groups treated with control diet both weaning on d 21 and d 28. However, supplementation with fermented diet did not affect the concentrations of short-chain fatty acids in the cecum (P > 0.05).CONCLUSION: Therefore, our data suggest that feed digestibility is improved in piglets weaned at 21 days, but intestinal barrier function is weaker than in piglets weaned at 28 days. However, compared with feeding control diet, supplementation with fermented diet both improved feed conversion and intestinal barrier function of weaned piglets by modulating intestinal microbiota.

Effects of Microbial Fermented Feed on Serum Biochemical Profile, Carcass Traits, Meat Amino Acid and Fatty Acid Profile, and Gut Microbiome Composition of Finishing Pigs

Microbial fermented feed is an important part of feed industry, while little research has focused on the solid-state fermentation of complete feed. Herein, the purpose of the present study was to investigate the effects of fermented complete feed (FCF) on the growth performance, biochemical profile, carcass traits, meat proximate composition, meat amino acid and fatty acid profile, and gut microbiome composition of finishing pigs, thereby providing references for the application of FCF in animal production. Twenty Duroc × Landrace × Yorkshire pigs with an average body weight (BW) of 48.74 ± 1.49 kg were divided randomly into control group (pigs received a basal diet, CN, n = 10) and FCF group (pigs fed with FCF, n = 10). The experiment lasted for 60 days. FCF improved the growth performance, which was indicated by a significantly increased final BW, average daily gain and average daily feed intake, and a significantly decreased feed-to-gain ratio. FCF improved biochemical profile, which was indicated by a higher alkaline phosphatase, glucose, immunoglobulin G, immunoglobulin M, superoxide dismutase, and total antioxidant capacity content. Pigs that received FCF had better carcass traits and meat quality than did pigs that received basal diet, which was indicated by a higher carcass length, crude protein content, lysine content, Glu content, C18:ln9c, C18:2n6c, C20:4n6, and unsaturated fatty acid content and a lower average back-fat thickness, C18:0, and saturated fatty acids. FCF significantly reduced the relative abundances of presumably pathogenic bacteria of phylum Proteobacteria and genus Escherichia–Shigella and enhanced the relative abundances of likely beneficial bacteria of phylum Firmicutes and genus Clostridium. In summary, FCF had a certain effect on the improvement of growth performance, serum biochemical profile, carcass traits, meat proximate composition, amino acid and fatty acid profile, and gut microbiome composition of finishing pigs.

The Combination of Indigofera Leaf and Corn as Fermented Feed on the Growth of Arabic Chicken (Gallus turcicus)

This study aims to find out the effect of combination of fermented Indegofera leaf and corn on the growth of Arabic chicken. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatment consisted of P.1: Commercial Feed; P.2: 80% Indegofera sp leaves + 20% Corn; P.3: 70% Indegofera leaves + 30% Corn; P.4: 60% Indegofera leaves + 40% Corn. Measurement of Arab chickens weight was carried out once a week for one month. The weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan's test. The results showed that the combination of fermented feed consisting Indegofera sp leaves and corn had a significant effect (P<0.05) on the growth of Arab chickens. The conclusion shows that fermented feed of Indegofera leaves sp and corn as chicken diet could improve the growth of Arabian chickens.

Effect of Pelleted Fermented Feed in Production Performance of Laying Hens

lactobacilli, Bacillus subtills, Bifidobacterium, Saccharomyces cerevisiae were applied to produce fermented feeds (FF), The FF was pelleted to investigated for its influences in production performance for laying hens, the (FF) was ferment with 10 g/kg feed of the probiotic with a wetting ratio of half a liter/kg of feed for 48 hours where it was used at rates 0, 25%, 50 %, 75%, and 100% for the treatments T1, T2, T3, T4, and T5 respectively, and the duration of the study lasted for seven periods, each period was two weeks, as for the following study results: Significant superiority (P<0.05) for treatment T5 during The first and sixth period and the treatment T4 during the second, third and fourth periods compared with the control treatment in the percentage of egg production, in egg weight significantly increased (P<0.05) of treatment T3 in the second, sixth and seventh periods, and treatment T4 during the third period exceeded treatment T5 during the fourth period, men while in the feed conversion factor, T4 treatment improved significantly (P≤0.05) during the third period and together with The treatment of T5 during the fourth period and all treatments of (FF) in the fifth period and the treatments T3, T5 in the sixth period and the treatment T3 in the seventh period, a significant (P<0.05) superiority was obtained for the treatments T4 and T5 during the second, third, fourth and sixth periods in the cumulative egg production and egg mass.

The supplementation of Fermented Feed (Indegofera Sp and Eurycoma longifolia) Leaves on Arabic Chicken Semen

This study aims to determine the effect of fermented feed in the form of Indegofera Sp and Eurycoma Longifolia (El) leaves on the quality of Arabian chicken semen. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments consisted of P.1 : Commercial Feed, P.2 : 80% Indegofera sp + 20% Eurycoma longifolia (El) leaves, P.3: 70% Indegofera sp leaves + 30% Eurycoma longifolia (El) leaves, P.4 : 60% Indegofera sp + 40% Eurycoma longifolia (El) leaves. Semen collection was carried out on day 30 after feeding. The semen quality were observed and analyzed using one-way analysis of variance (ANOVA) followed by Duncan&#39;s test. The results showed that the combination of two fermented feed (Indegofera Sp and Eurycoma Longifolia) leaves had a significant effect (P&lt;0.05) on the quality of arab chicken spermatozoa. The fermented feed is able to improve the quality of Arabic chicken semen.