scholarly journals Concentrate feeding and ruminal fermentation. 2. Influence of concentrate ingredients on pH and on L-lactate concentration in incubations in vitro with rumen fluid.

1982 ◽  
Vol 30 (4) ◽  
pp. 259-274
Author(s):  
A. Malestein ◽  
A.T. van 't Klooster ◽  
G.H.M. Counotte ◽  
R.A. Prins
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Rumen Fluid ◽  
Lactate Concentration ◽  
Lactic Acid Concentration ◽  
Ruminal Fermentation ◽  
Beet Pulp ◽  
Effect Of Particle Size ◽  
Maize Meal

2. Rumen fluid was sampled before feeding from cows given hay, diluted with an anaerobic salt solution and added (20 ml) to different amounts (mostly 1 g) of maize gluten meal, maize, citrus pulp, tapioca, beet pulp, coconut expeller or soya bean oilmeal for incubation at 39 deg C. After at least 4 h of incubation there were large differences in pH and lactic acid concentration. The acidotic index of the feeds was influenced by increasing concentration of the substrate. Except with maize meal, there was little effect of particle size on pH and lactic acid concentration. There were differences in effect on pH and lactic acid concentration between different batches of the same feeds, especially with maize meal. Incubations with mixtures of concentrate ingredients showed different pH and lactic acid concentrations from values expected from results with the single ingredients. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Concentrate feeding and ruminal fermentation. 3. Influence of concentrate ingredients on pH, on DL-lactic acid concentration in rumen fluid of dairy cows and on dry matter intake.

1984 ◽  
Vol 32 (1) ◽  
pp. 9-21
Author(s):  
A. Malestein ◽  
A.T. van't Klooster ◽  
R.A. Prins ◽  
G.H.M. Counotte
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Lactate Concentration ◽  
Dry Matter Intake ◽  
Lactic Acid Concentration ◽  
Ruminal Fermentation ◽  
Citrus Pulp ◽  
Maize Meal ◽  
Gluten Feed

Concentrate ingredients (maize meal, coconut oilmeal, maize gluten feed, citrus pulp, tapioca and soya bean oilmeal) were given daily at 0900 h into the rumen of 4 cows for 5 successive days. Hay was given freely at 1300 h. Between experimental periods there were intervals of at least 17 days when hay was given at 0800 and 1700 h. In a second experiment 6-kg amounts of 1:1 mixtures of 2 ingredients were given. Lowest pH and highest concentrations of D- and L-lactate in rumen fluid were reached 2 to 3 h after ingredients were given. Lowest pH and highest D- and L-lactate concentration in rumen fluid relative to the situation after citrus pulp was given, taken together as the potential risk of lactic acid acidosis (RLA), was best correlated with the content of glucose plus fructose (GF) and soluble protein (SPROT); RLA = 1.47GF + 0.49SPROT + 22.50 (r = 0.89). When combined ingredients were given, pH and D- plus L-lactate concentrations differed from values calculated from results after the same ingredients had been given separately. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Effect of Palm Kernel Meal and Malic Acid on Rumen Characteristics of Growing Naemi Lambs Fed Total Mixed Ration

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 408 ◽  
Author(s):  
Mutassim M. Abdelrahman ◽  
Ibrahim Alhidary ◽  
Hani H. Albaadani ◽  
Mohsen Alobre ◽  
Rifat Ullah Khan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Malic Acid ◽  
Rumen Fluid ◽  
Palm Kernel ◽  
Lactic Acid Concentration ◽  
Total Mixed Ration ◽  
Rumen Ph ◽  
Kernel Meal ◽  
Palm Kernel Meal

This study was conducted to investigate the effect of malic acid and 20% palm kernel meal (PKM) on ruminal characteristics. A total of 32 growing lambs were randomly distributed into control (barley and alfalfa ha), total mixed ration (T1), TMR + 20% PKM (T2), TMR + PKC 20% + 4 mL/day malic acids (T3). Lambs were fed these diets ad libitum for 84 days. The results showed that propionic acid in the rumen fluid increased significantly (p < 0.05) in T1 and T3. Lactic acid concentration of rumen fluid increased significantly (p < 0.05) in T2 while the pH increased significantly. The coloration of rumen and reticulum was improved in T3. In addition, most of the histomorphological features were higher in T3 and T2. We concluded that the addition of malic acid supplementation to lambs fed PKC caused a significant improvement in the rumen pH and decreased lactic acid concentration in growing Naemi lambs.

scholarly journals Correlation Between Lactic Acid Concentration and The Severity of Neonatal Sepsis

2019 ◽  
Vol 26 (1) ◽  
pp. 55
Author(s):  
Agustin Iskandar ◽  
Nada Putri Pranidya ◽  
Eko Sulistijono ◽  
Aryati Aryati
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Neonatal Sepsis ◽  
Lactate Level ◽  
Lactate Concentration ◽  
Infant Mortality Rate ◽  
Observational Research ◽  
Lactic Acid Concentration ◽  
Mortality Outcome ◽  
Lactate Levels

BackgroundsNeonatal sepsis remains a major cause of high infant mortality rate in Indonesia, and served as one of risk factor for early neonatal mortality. This study aims to determine the relationship between lactate levels andthe severity of sepsis and prognostic value of lactic acid as a predictor of severity in neonatal sepsis.. MethodsThis research is analytic observational research using cross sectional method. The subjects were patients of neonatal sepsis treated in the Perinatology Room of Saiful Anwar Hospital Malang from February to June 2015. Lactic acid concentration in plasma was measured by using enzymatic colorimetric  methodResults and DiscussionThe results showed a positive and significant correlation (p = 0,023; r= o,414) between lactate concentration and severity of sepsis, where every increase of sepsis degree increased lactate level of 0,151 mmol / L. Whereas by using  cut off ≥ 2.5 mmol / L, the prognostic test showed  66.7% of sensitivity and 76.2% of specificity. The chi square test of lactate> 2 mmol / L and <2 mmol / L showed Odd Ratio (OR) of 1,3 whereas at lactate level > 5 mmol / L and <5 mmol / L showed OR of 4,8 indicated that although there was no difference but the mortality outcomes of neonatal sepsis 4.8 times greater those of life. Thus lactate levelsof > 5 mmol / L can determine mortality outcome of neonatal sepsis.Conclusions and suggestionsThe higher the lactic acid level the more severe of neonatal sepsis. Lactate levels greater than 5 mmol / L was predictor for determining mortality outcome in neonatal sepsis

EFFECT OF LASALOCID, MONENSIN AND THIOPEPTIN ON LACTATE PRODUCTION FROM IN VITRO RUMEN FERMENTATION OF STARCH

1986 ◽  
Vol 66 (1) ◽  
pp. 129-139 ◽  
Author(s):  
T. G. NAGARAJA ◽  
S. J. GALITZER ◽  
D. L. HARMON ◽  
S. M. DENNIS
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Lactate Concentration ◽  
Lactate Production ◽  
Starch Fermentation ◽  
Key Words Antibiotics ◽  
Lactate Fermentation ◽  
Set Up ◽  
In Vitro Rumen Fermentation

Starch fermentations with strained rumen fluid and centrifuged rumen fluid devoid of protozoa were set up to test the effect of lasalocid, monensin, and thiopeptin on L(+) and D(−) lactate production. Protozoa-free rumen fluid was the supernatant from low-speed centrifugation of strained rumen fluid. Starch fermentation in the control (no antibiotic) with centrifuged rumen fluid resulted in higher lactate concentration than the fermentation with strained rumen fluid. Decreased lactate production with strained rumen fluid was attributed to sequestration of starch by protozoa and to enhanced lactate fermentation. Addition of lasalocid or monensin (1.5–48.0 μg mL−1) to the fermentation enhanced L(+) and D(−) lactate production in the presence of protozoa. In the absence of protozoa, lasalocid and monensin inhibited L(+) lactate production; however, D(−) lactate concentration was unaffected. Increased lactate production by lasalocid and monensin in the presence of protozoa was possibly due to inhibition of protozoal engulfment of starch. Thiopeptin had no effect on lactate production in the presence of protozoa but in the absence of protozoa lactate production was inhibited. Similar antibiotic responses were observed at different starch amounts (0.5, 1.5 and 3.0 g) and with starch types (soluble, corn and wheat) and with rumen fluid collected from defaunated cattle. Key words: Antibiotics, cattle, rumen, starch, fermentation, lactic acid

scholarly journals Concentration Dependent Influence of Lipopolysaccharides on Separation of Hoof Explants and Supernatant Lactic Acid Concentration in an Ex Vivo/In Vitro Laminitis Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143754 ◽  
Author(s):  
Nicole Reisinger ◽  
Simone Schaumberger ◽  
Veronika Nagl ◽  
Sabine Hessenberger ◽  
Gerd Schatzmayr
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Ex Vivo ◽  
Lactic Acid Concentration

Blood osmolality in vitro: dependence on PCO2, lactic acid concentration, and O2 saturation

1983 ◽  
Vol 54 (1) ◽  
pp. 118-122 ◽  
Author(s):  
D. Boning ◽  
N. Maassen
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Freezing Point ◽  
Co2 Absorption ◽  
Lactic Acid Concentration ◽  
Co2 Partial Pressure ◽  
Constant Ph ◽  
Acid Titration ◽  
Point Determination

Changes of osmolality (Osm) were measured by freezing-point determination in true plasma of 10 healthy subjects. This was done after equilibration with CO2 (0.5–10.0%), after the addition of lactic acid (10 and 20 mmol/l), and after deoxygenation. The graph for the dependence of Osm on CO2 partial pressure (PCO2) in oxygenated blood resembles the classical CO2 absorption curve. The increase of Osm with PCO2 (approximately 0.2 mosmol . kg H2O-1 . Torr-1) is almost as great as the increase in dissolved CO2 plus bicarbonate (HCO-3). Addition of lactic acid shifts the curve upward by only 0.6 mosmol/mmol because of displacement of HCO-3. Deoxygenation has no significant effect at constant PCO2 despite an increase in [HCO-3]. This is probably due to the binding of 2,3-diphosphoglycerate to hemoglobin. It can be seen in the Osm-pH diagram that differences between CO2 and lactic acid titration largely disappear. For each lactic acid concentration there is a linear dependence corresponding to the linear [HCO-3]-pH relation in plasma. At constant pH, Osm increases after deoxygenation. The observed in vitro relation might explain part of the osmolality increase during physical exercise.

Effect of water quality on in vitro fermentation of sorghum and barley for poultry diets

2007 ◽  
Vol 2007 ◽  
pp. 243-243
Author(s):  
A.T. Niba ◽  
J.D. Beal ◽  
A.C. Kudi ◽  
P.H. Brooks
Keyword(s):  
Water Quality ◽  
Lactic Acid ◽  
Acid Concentration ◽  
Lactic Acid Concentration ◽  
In Vitro Fermentation ◽  
Effect Of Water ◽  
Fermented Feed ◽  
Fermentation Pattern ◽  
Poultry Diets

Low pH and high lactic acid concentration of fermented feed has been reported to be responsible for the antimicrobial activity of fermented feeds (Brooks et al., 2001). For example, to prevent the growth of Salmonella spp. in liquid feeds, a threshold lactic acid concentration of 75mM is required (Beal et al., 2002). Therefore, factors that are likely to affect the production of lactic acid during fermentation will have important implications for the ability of such feeds to withstand colonisation by pathogens. The objective of the present study was to investigate the effect of water quality on the fermentation pattern of sorghum and barley.

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