Instrumental, theoretical, and experimental aspects of determining thermodynamic and kinetic parameters from steady-state and non-steady-state cyclic voltammetry at microelectrodes in high-resistance solvents: application to the fac/mer-[Cr(CO)3(.eta.3-Ph2PCH2CH2P(Ph)CH2CH2PPh2)]+/0 square reaction scheme in dichloromethane

1992 ◽  
Vol 64 (9) ◽  
pp. 1014-1021 ◽  
Author(s):  
Alan M. Bond ◽  
Stephen W. Feldberg ◽  
Howard B. Greenhill ◽  
Peter J. Mahon ◽  
Ray. Colton ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Steady State ◽  
Kinetic Parameters ◽  
High Resistance ◽  
Reaction Scheme ◽  
Thermodynamic And Kinetic Parameters

scholarly journals Steady state kinetic parameters for the thrombin-catalyzed conversion of human fibrinogen to fibrin.

1983 ◽  
Vol 258 (15) ◽  
pp. 9276-9282 ◽  
Author(s):  
D L Higgins ◽  
S D Lewis ◽  
J A Shafer
Keyword(s):  
Steady State ◽  
Kinetic Parameters ◽  
Human Fibrinogen ◽  
Steady State Kinetic ◽  
State Kinetic

scholarly journals Characterization of Nicotinamidases: Steady State Kinetic Parameters, Classwide Inhibition by Nicotinaldehydes, and Catalytic Mechanism

Biochemistry ◽  
2010 ◽  
Vol 49 (49) ◽  
pp. 10421-10439 ◽  
Author(s):  
Jarrod B. French ◽  
Yana Cen ◽  
Tracy L. Vrablik ◽  
Ping Xu ◽  
Eleanor Allen ◽  
...  
Keyword(s):  
Steady State ◽  
Kinetic Parameters ◽  
Catalytic Mechanism ◽  
Steady State Kinetic ◽  
State Kinetic

scholarly journals Variation in the Steady State Kinetic Parameters of Wild Type and Mutant T5 5′-3′-Exonuclease With pH

1999 ◽  
Vol 274 (25) ◽  
pp. 17711-17717 ◽  
Author(s):  
Timothy J. Pickering ◽  
Scott Garforth ◽  
Jon R. Sayers ◽  
Jane A. Grasby
Keyword(s):  
Steady State ◽  
Kinetic Parameters ◽  
Wild Type ◽  
Steady State Kinetic ◽  
State Kinetic

Estimation of kinetic parameters of androgen-synthesizing enzyme activities in superfused Leydig cells from rat testes: Difference between endogenous and exogenous substrates

1984 ◽  
Vol 4 (6) ◽  
pp. 483-488 ◽  
Author(s):  
Nikolaus Kühn-Velten ◽  
Joachim Wolff ◽  
Wolfgang Staib
Keyword(s):  
Steady State ◽  
Kinetic Parameters ◽  
Active Site ◽  
Enzyme Activities ◽  
Substrate Concentration ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Endogenous Substrate ◽  
Catalyzed Reactions ◽  
Actual Substrate

Kinetic parameters of 3β-hydroxysteroid dehydrogenase/isomerase, steroid-17α-monooxygenase, and steroid-17,20-lyase activities were estimated under steady-state conditions. Purified Leydig cells from rat testes were superfused with pregnenolone, progesterone, or 17α-hydroxyprogesterone. The Km values for both the monooxygenase- and the lyase-catalyzed reactions were by factors of five to ten higher if analyzed with the exogenously added substrate (0.98 and 0.65 μM, respectively) than if calculated from endogenous substrate derived from a precursor (0.10 and 0.13 μM, respectively). This discrepancy may be explained by different substrate partition between the intra- and extraceIJular spaces and by different substrate concentration at the active site of the respective enzyme, depending on whether the actual substrate is of exogenous or endogenous source.

scholarly journals Contribution of the intra- and intermolecular routes in autocatalytic zymogen activation: application to pepsinogen activation.

2006 ◽  
Vol 53 (2) ◽  
pp. 407-420 ◽  
Author(s):  
Ramón Varón ◽  
Matilde E Fuentes ◽  
Manuela García-Moreno ◽  
Francisco Garcìa-Sevilla ◽  
Enrique Arias ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Rate Constants ◽  
Time Course ◽  
Initial Conditions ◽  
Reaction Scheme ◽  
Complete Analysis ◽  
Zymogen Activation ◽  
Relative Contribution ◽  
Starting Point ◽  
Definition Of

Taking as the starting point a recently suggested reaction scheme for zymogen activation involving intra- and intermolecular routes and the enzyme-zymogen complex, we carry out a complete analysis of the relative contribution of both routes in the process. This analysis suggests the definition of new dimensionless parameters allowing the elaboration, from the values of the rate constants and initial conditions, of the time course of the contribution of the two routes. The procedure mentioned above related to a concrete reaction scheme is extrapolated to any other model of autocatalytic zymogen activation involving intra- and intermolecular routes. Finally, we discuss the contribution of both of the activating routes in pepsinogen activation into pepsin using the values of the kinetic parameters given in the literature.

Thermodynamic and kinetic parameters and high rate discharge-ability of the AB5-type metal hydride anode

2016 ◽  
Vol 41 (23) ◽  
pp. 9914-9923 ◽  
Author(s):  
Abbes Kaabi ◽  
Chokri Khaldi ◽  
Jilani Lamloumi
Keyword(s):  
Kinetic Parameters ◽  
Metal Hydride ◽  
High Rate ◽  
Rate Discharge ◽  
Thermodynamic And Kinetic Parameters ◽  
High Rate Discharge

Determination of Reaction Scheme and Kinetic Parameters

1986 ◽  
pp. 293-339
Author(s):  
Satoru Kuhara ◽  
Kiyokazu Nemoto ◽  
Yukihiro Eguchi ◽  
Naoto Sakamoto
Keyword(s):  
Kinetic Parameters ◽  
Reaction Scheme
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