Effect of mobile phase composition and highly fluorinated bonded phases on the apparent free energy of transfer of solute functional groups

1987 ◽  
Vol 59 (7) ◽  
pp. 1032-1039 ◽  
Author(s):  
Paul C. Sadek ◽  
Peter W. Carr ◽  
Michael J. Ruggio
Keyword(s):  
Free Energy ◽  
Phase Composition ◽  
Functional Groups ◽  
Mobile Phase ◽  
Mobile Phase Composition ◽  
Bonded Phases ◽  
Free Energy Of Transfer ◽  
Energy Of Transfer ◽  
Apparent Free Energy

Sequential Injection Chromatography with Monolithic Column for Phenothiazines Assay in Human Urine and Pharmaceutical Formulations

2020 ◽  
Vol 16 (7) ◽  
pp. 967-975
Author(s):  
Abubakr M. Idris
Keyword(s):  
Phase Composition ◽  
Human Urine ◽  
Mobile Phase ◽  
Monolithic Column ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Assay Method ◽  
Sequential Injection ◽  
Mobile Phase Composition ◽  
Sequential Injection Chromatography

Methods: Sequential injection chromatography (SIC) with monolithic column has been proposed with potential benefits for separation and quantification. Objective: To utilize SIC to develop a new assay method for the separation and quantification of some phenothiazines (promethazine, chlorpromazine and perphenazine) in human urine and synthetic pharmaceutical formulations. Methods: The 32 full-factorial design was adopted to study the effect of mobile phase composition on separation efficiency, retention time, peak height and baseline. The separation was conducted on a C18 monolithic column (100 × 4.6 mm) using a mobile phase composition of phosphate: acetonitrile:methanol (60:28:12) at pH 4.0. The detection was carried out using a miniaturized fiber optic spectrometer at 250 nm. Results: Satisfactory analytical features, including number of theoretical plates (1809-6232), peak symmetry (1.0-1.3), recovery (95.5-99.1% in pharmaceutical formulations and 91.6-94.7% in urine), intra-day precision (0.36-1.60% for pharmaceutical formulation and 2.96-3.67 for urine), inter-day precision (1.47-2.28% for pharmaceutical formulation), limits of detection (0.23-0.88 μg/ml) and limits of quantification (0.77-2.90 μg/ml), were obtained. Conclusion: The remarkable advantages of the proposed SIC method are the inexpensiveness in terms of instrumentation and reagent consumption.

Dependence of the capacity ratio on mobile phase composition in liquid adsorption chromatography

1978 ◽  
Vol 11 (10) ◽  
pp. 581-585 ◽  
Author(s):  
M. Jaroniec ◽  
J. Narkiewicz ◽  
M. Borówko
Keyword(s):  
Phase Composition ◽  
Mobile Phase ◽  
Adsorption Chromatography ◽  
Mobile Phase Composition ◽  
Liquid Adsorption ◽  
Capacity Ratio ◽  
Liquid Adsorption Chromatography

The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals

2010 ◽  
Vol 99 (4) ◽  
pp. 1674-1692 ◽  
Author(s):  
Tsutomu Arakawa ◽  
Daisuke Ejima ◽  
Tiansheng Li ◽  
John S. Philo
Keyword(s):  
Phase Composition ◽  
Size Exclusion Chromatography ◽  
Mobile Phase ◽  
Critical Role ◽  
Size Exclusion ◽  
Mobile Phase Composition ◽  
Exclusion Chromatography ◽  
Protein Pharmaceuticals

Solvation in hydroorganic media—I. Gibbs free energy of transfer for RbCl from cationic glass electrode measurements

1976 ◽  
Vol 21 (6) ◽  
pp. 425-430 ◽  
Author(s):  
R. Smits ◽  
D.L. Massart ◽  
J. Juillard ◽  
J.-P. Morel
Keyword(s):  
Free Energy ◽  
Gibbs Free Energy ◽  
Glass Electrode ◽  
Free Energy Of Transfer ◽  
Energy Of Transfer

Microemulsion as Model to Predict Free Energy of Transfer of Electrolyte in Solvent Extraction

2021 ◽  
pp. 1-36
Author(s):  
Simon Gourdin-Bertin ◽  
Jean-François Dufrêche ◽  
Magali Duvail ◽  
Thomas Zemb
Keyword(s):  
Free Energy ◽  
Solvent Extraction ◽  
Free Energy Of Transfer ◽  
Energy Of Transfer
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