scholarly journals Biosensors Based on Membrane-Bound Enzymes Immobilized in a 5-(Octyldithio)-2-nitrobenzoic Acid Layer on Gold Electrodes

2000 ◽  
Vol 72 (22) ◽  
pp. 5753-5753 ◽  
Author(s):  
M. Darder ◽  
E. Casero ◽  
F. Pariente ◽  
E. Lorenzo
Keyword(s):  
Gold Electrodes ◽  
Nitrobenzoic Acid ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

Biosensors Based on Membrane-Bound Enzymes Immobilized in a 5-(Octyldithio)-2-nitrobenzoic Acid Layer on Gold Electrodes

2000 ◽  
Vol 72 (16) ◽  
pp. 3784-3792 ◽  
Author(s):  
M. Darder ◽  
E. Casero ◽  
F. Pariente ◽  
E. Lorenzo
Keyword(s):  
Gold Electrodes ◽  
Nitrobenzoic Acid ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

Some Aspects of the Polyhexamethyleneguanidine Salts Effect on Cell Cultures

2014 ◽  
Vol 1 (1) ◽  
pp. 62-67 ◽  
Author(s):  
M. Mandygra ◽  
A. Lysytsia
Keyword(s):  
Proliferative Activity ◽  
Cell Monolayer ◽  
Eukaryotic Cell ◽  
Culture Methods ◽  
Cellular Processes ◽  
Negative Effect ◽  
Membrane Bound ◽  
Membrane Bound Enzymes ◽  
Anion Type ◽  
Cell Culture Methods

Aim. To investigate the effect of polyhexamethyleneguanidine (PHMG) to eukaryotic cell culture. Methods. The passaged bovine tracheal cells culture (TCC) and primary culture of chicken embryo fi broblasts (FCE) were used in the experiments. TCC and FCE monolayers were treated with aqueous solutions of PHMG chloride or succinate. The method of PHMG polycation adsorption to the cells’ plasma membrane together with microscopy were applied. Results. The dependence of PHMG effect on the eukaryotic cells on the agent concentration, duration of exposure and the anion type has been fi xed. The PHMG concentration of 10 –5 per cent (0.1 μg/ml) never causes degradation of the previously formed cell monolayer, while the higher concentrations damage it. The conditions of the PHMG chloride and succinate’s negative effect on cell proliferation and inhibition of monolayer formation were determined. The hypothesis that under certain conditions PHMG stimulates the proliferative activity of the cells has been confi rmed. Stimulation may be associated with non-specifi c stress adaptation of cells. In this case, it is due to modifi cations of the cell membrane after PHMG adsorption to it. Conclusions. PHMG polycation binds with the membrane’s phosphoglycerides fi rmly and irreversibly. A portion of the lipids are removed from participation in the normal cellular processes at that. At the same time, the synthesis of new lipids and membrane-bound enzymes is probably accelerated. The phospholip ids’ neogenesis acceleration can stimulate mitosis under certain conditions. The obtained results can be used in the biotechnologies.

scholarly journals Regulation of membrane-bound enzymes of glycosphingolipid biosynthesis

1984 ◽  
Vol 25 (13) ◽  
pp. 1541-1547
Author(s):  
J D Burczak ◽  
R M Soltysiak ◽  
C C Sweeley
Keyword(s):  
Membrane Bound ◽  
Membrane Bound Enzymes ◽  
Glycosphingolipid Biosynthesis

Retinol dehydrogenases: Membrane-bound enzymes for the visual function

2014 ◽  
Vol 92 (6) ◽  
pp. 510-523 ◽  
Author(s):  
Mustapha Lhor ◽  
Christian Salesse
Keyword(s):  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Membrane Binding ◽  
Large Family ◽  
Structural Features ◽  
Visual Cycle ◽  
Retinoid Metabolism ◽  
Current State ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

Retinoid metabolism is important for many physiological functions, such as differenciation, growth, and vision. In the visual context, after the absorption of light in rod photoreceptors by the visual pigment rhodopsin, 11-cis retinal is isomerized to all-trans retinal. This retinoid subsequently undergoes a series of modifications during the visual cycle through a cascade of reactions occurring in photoreceptors and in the retinal pigment epithelium. Retinol dehydrogenases (RDHs) are enzymes responsible for crucial steps of this visual cycle. They belong to a large family of proteins designated as short-chain dehydrogenases/reductases. The structure of these RDHs has been predicted using modern bioinformatics tools, which allowed to propose models with similar structures including a common Rossman fold. These enzymes undergo oxidoreduction reactions, whose direction is dictated by the preference and concentration of their individual cofactor (NAD(H)/NADP(H)). This review presents the current state of knowledge on functional and structural features of RDHs involved in the visual cycle as well as knockout models. RDHs are described as integral or peripheral enzymes. A topology model of the membrane binding of these RDHs via their N- and (or) C-terminal domain has been proposed on the basis of their individual properties. Membrane binding is a crucial issue for these enzymes because of the high hydrophobicity of their retinoid substrates.

Ultracytochemical localization of membrane-bound enzymes in the node of Ranvier

1989 ◽  
Vol 20 (3-4) ◽  
pp. 207-215
Author(s):  
Kenichirou Inomata ◽  
Fumio Nasu ◽  
Kazuhiro Tomiyasu
Keyword(s):  
Node Of Ranvier ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

scholarly journals Formation of 4-Keto-D-aldopentoses and 4-Pentulosonates (4-Keto-D-pentonates) with Unidentified Membrane-Bound Enzymes from Acetic Acid Bacteria

2011 ◽  
Vol 75 (9) ◽  
pp. 1801-1806 ◽  
Author(s):  
Osao ADACHI ◽  
Roque A. HOURS ◽  
Emiko SHINAGAWA ◽  
Yoshihiko AKAKABE ◽  
Toshiharu YAKUSHI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Acetic Acid Bacteria ◽  
Membrane Bound ◽  
Membrane Bound Enzymes
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