scholarly journals A clickable glutamine (CliQ) derivative for the traceless reversible modification of peptides and proteins

2019 ◽  
Vol 55 (14) ◽  
pp. 2043-2045
Author(s):  
Samuel D. Whedon ◽  
Marissa K. Parker ◽  
Elizabeth L. Tyson ◽  
Tobias Ritterhoff ◽  
Patrick M. M. Shelton ◽  
...  
Keyword(s):  
Click Reaction ◽  
Complex Mixtures ◽  
Selective Isolation ◽  
Affinity Tags ◽  
Reversible Modification

The Cu(i)-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures.

Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags

2004 ◽  
Vol 76 (14) ◽  
pp. 4104-4111 ◽  
Author(s):  
Yu Lu ◽  
Patricia Bottari ◽  
Frantisek Turecek ◽  
Ruedi Aebersold ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Specific Proteins

Design and Synthesis of Visible Isotope-Coded Affinity Tags for the Absolute Quantification of Specific Proteins in Complex Mixtures

2004 ◽  
Vol 15 (2) ◽  
pp. 380-388 ◽  
Author(s):  
Patricia Bottari ◽  
Ruedi Aebersold ◽  
Frantisek Turecek ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Design And Synthesis ◽  
Specific Proteins ◽  
The Absolute

scholarly journals Reversible modification of oligodeoxynucleotides: click reaction at phosphate group and alkali treatment

2011 ◽  
Vol 52 (52) ◽  
pp. 7135-7137 ◽  
Author(s):  
Kazuhito Tanabe ◽  
Yuichirou Ando ◽  
Sei-ichi Nishimoto
Keyword(s):  
Click Reaction ◽  
Alkali Treatment ◽  
Phosphate Group ◽  
Reversible Modification

scholarly journals Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags

2007 ◽  
pp. 159-176
Author(s):  
Yu Lu ◽  
Patricia Bottari ◽  
Ruedi Aebersold ◽  
Frantisek Turecek ◽  
Michael H. Gelb
Keyword(s):  
Absolute Quantification ◽  
Complex Mixtures ◽  
Affinity Tags ◽  
Specific Proteins

A method for selective separation of scandium (III) from yttrium and a number of rare-earth elements for its subsequent determination

2018 ◽  
Vol 84 (11) ◽  
pp. 23-27
Author(s):  
M. I. Degtev ◽  
A. A. Yuminova ◽  
A. S. Maksimov ◽  
A. P. Medvedev
Keyword(s):  
Rare Earth ◽  
Aqueous Phase ◽  
Selective Isolation ◽  
Optimum Ratio ◽  
Extraction Condition ◽  
Sulfosalicylic Acid ◽  
Salting Out ◽  
Subsequent Determination ◽  
Toxic Organic Solvents ◽  
Metal Ions Extraction

The possibility of using an aqueous stratified system of antipyrine — sulfosalicylic acid — water for the selective isolation of scandium macro- and microquantities for subsequent determination is studied. The proposed extraction system eliminates the usage of toxic organic solvents. The organic phase with a volume of 1.2 to 2.0 ml, resulting from delamination of the aqueous phase containing antipyrine and sulfosalicylic acid is analysed to assess the possibility of using such systems for metal ions extraction. Condition necessary for the formation of such a phase were specified: the ratio of the initial components, their concentration, presence of inorganic salting out agents. The optimum ratio of antipyrine to sulfosalicylic acid is 2:1 at concentrations of 0.6 and 0.3 mol/liter in a volume of the aqueous phase of 10 ml. The obtained phase which consists of antipyrinium sulfosalicylate, free antipyrine and water, quantitatively extracts macro- and microquantities of scandium at pH = 1.54. Macro- and microquantities of yttrium, terbium, lanthanum, ytterbium and gadolinium are not extracted under the aforementioned conditions thus providing selective isolation of scandium from the bases containing yttrium, ytterbium, terbium, lanthanum, and gadolinium.

Synthetic Routes for 1,4-disubstituted 1,2,3-triazoles: A Review

2019 ◽  
Vol 23 (8) ◽  
pp. 860-900 ◽  
Author(s):  
Chander P. Kaushik ◽  
Jyoti Sangwan ◽  
Raj Luxmi ◽  
Krishan Kumar ◽  
Ashima Pahwa
Keyword(s):  
Solid Phase ◽  
Click Reaction ◽  
Biological Activities ◽  
Triazole Ring ◽  
Copper Catalysts ◽  
Biologically Active ◽  
Pharmaceutical Chemistry ◽  
Biological Targets ◽  
Synthetic Routes ◽  
High Yields

N-Heterocyclic compounds like 1,2,3-triazoles serve as a key scaffolds among organic compounds having diverse applications in the field of drug discovery, bioconjugation, material science, liquid crystals, pharmaceutical chemistry and solid phase organic synthesis. Various drugs containing 1,2,3-triazole ring which are commonly available in market includes Rufinamide, Cefatrizine, Tazobactam etc., Stability to acidic/basic hydrolysis along with significant dipole moment support triazole moiety for appreciable participation in hydrogen bonding and dipole-dipole interactions with biological targets. Huisgen 1,3-dipolar azide-alkyne cycloaddition culminate into a mixture of 1,4 and 1,5- disubstituted 1,2,3-triazoles. In 2001, Sharpless and Meldal came across with a copper(I) catalyzed regioselective synthesis of 1,4-disubstituted 1,2,3-triazoles by cycloaddition between azides and terminal alkynes. This azide-alkyne cycloaddition has been labelled as a one of the important key click reaction. Click synthesis describes chemical reactions that are simple to perform, gives high selectivity, wide in scope, fast reaction rate and high yields. Click reactions are not single specific reaction, but serve as a pathway for construction of simple to complex molecules from a variety of starting materials. In the last few decades, 1,2,3-triazoles attracted attention of researchers all over the world because of their broad spectrum of biological activities. Keeping in view the biological importance of 1,2,3-triazole, in this review we focus on the various synthetic routes for the syntheisis of 1,4-disubstituted 1,2,3-triazoles. This review involves various synthetic protocols which involves copper and non-copper catalysts, different solvents as well as substrates. It will boost synthetic chemists to explore new pathway for the development of newer biologically active 1,2,3-triazoles.

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

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