Multiplexed Rectilinear Ion Trap Mass Spectrometer for High-Throughput Analysis

2006 ◽  
Vol 78 (14) ◽  
pp. 4830-4838 ◽  
Author(s):  
Amy M. Tabert ◽  
Michael P. Goodwin ◽  
Jason S. Duncan ◽  
Charles D. Fico ◽  
R. Graham Cooks
Keyword(s):  
Mass Spectrometer ◽  
High Throughput ◽  
Ion Trap ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Ion Trap Mass Spectrometer

scholarly journals High-Throughput LC/MS/MS Analysis of Ruscogenin and Neoruscogenin in Ruscus aculeatus L.

2009 ◽  
Vol 92 (4) ◽  
pp. 1055-1059 ◽  
Author(s):  
Laurian Vlase ◽  
Béla Kiss ◽  
Georgeta Balica ◽  
Mircea Tas ◽  
Gianina Crisan ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectrometer ◽  
High Throughput ◽  
Ion Trap ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Ionization Source ◽  
Ion Trap Mass Spectrometer ◽  
Multiple Reaction Monitoring Mode ◽  
Ionization Mode

Abstract A new, sensitive LC/MS/MS method was developed for the quantification of ruscogenin and neoruscogenin in hydrolyzed extracts from Ruscus aculeatus L. (Liliaceae). The two sapogenins were separated on a Zorbax SB-C18 column under isocratic conditions. The detection was performed in the multiple reaction monitoring mode using an ion trap mass spectrometer with an electrospray ionization source operated in positive ionization mode. For the quantification of the ruscogenin and neoruscogenin, calibration curves were constructed over the range of 21000 ng/mL. This is the first reported LC/MS/MS method for the simultaneous analysis of ruscogenin and neoruscogenin, and it showed superior sensitivity when compared with other assays described in the literature. The method has been successfully applied to quantify the two sapogenins in aerial (phylloclades) and underground parts (rhizomes, roots) of Ruscus aculeatus L.

scholarly journals High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer

2017 ◽  
Vol 16 (5) ◽  
pp. 2072-2079 ◽  
Author(s):  
Timothy P. Cleland ◽  
Caroline J. DeHart ◽  
Ryan T. Fellers ◽  
Alexandra J. VanNispen ◽  
Joseph B. Greer ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Orbitrap Mass Spectrometer ◽  
Human Proteins

Combination of Free Software, Light Microscopy and Non-specific Dyes for a High-throughput Analysis in Live/Dead Cell Count

2015 ◽  
Vol 11 (4) ◽  
pp. 233-238 ◽  
Author(s):  
Luciano Cardoso ◽  
Suellen Cordeiro ◽  
Marcio Fronza ◽  
Denise Endringer ◽  
Tadeu de Andrade ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Cell Count ◽  
High Throughput ◽  
Free Software ◽  
Dead Cell ◽  
High Throughput Analysis ◽  
Throughput Analysis

Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell–ECM Interactions

2021 ◽  
Author(s):  
Ruoxing Lei ◽  
Erin A. Akins ◽  
Kelly C. Y. Wong ◽  
Nicole A. Repina ◽  
Kayla J. Wolf ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals Quadrupole Ion Trap Mass Spectrometer for Ice Giant Atmospheres Exploration

2021 ◽  
Vol 217 (1) ◽  
Author(s):  
J. Simcic ◽  
D. Nikolić ◽  
A. Belousov ◽  
D. Atkinson ◽  
C. Lee ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Ion Trap ◽  
Quadrupole Ion Trap ◽  
Atomic Mass ◽  
Charge Ratio ◽  
Inlet System ◽  
Mass Spectrometers ◽  
Ion Trap Mass Spectrometer ◽  
Entry Probe ◽  
Composition Measurements

AbstractTo date, a variety of different types of mass spectrometers have been utilized on missions to study the composition of atmospheres of solar system bodies, including Venus, Mars, Jupiter, Titan, the moon, and several comets. With the increasing interest in future small probe missions, mass spectrometers need to become even more versatile, lightweight, compact, and sensitive.For in situ exploration of ice giant atmospheres, the highest priority composition measurements are helium and the other noble gases, noble gas isotopes, including 3He/4He, and other key isotopes like D/H. Other important but lower priority composition measurements include abundances of volatiles C, N, S, and P; isotopes 13C/12C, 15N/14N, 18O/17O/16O; and disequilibrium species PH3, CO, AsH3, GeH4, and SiH4. Required measurement accuracies are largely defined by the accuracies achieved by the Galileo (Jupiter) probe Neutral Mass Spectrometer and Helium Abundance Detectors, and current measurement accuracies of solar abundances.An inherent challenge of planetary entry probe mass spectrometers is the introduction of material to be sampled (gas, solid, or liquid) into the instrument interior, which operates at a vacuum level. Atmospheric entry probe mass spectrometers typically require a specially designed sample inlet system, which ideally provides highly choked, nearly constant mass-flow intake over a large range of ambient pressures. An ice giant descent probe would have to operate for 1-2 hours over a range of atmospheric pressures, possibly covering 2 or more orders of magnitude, from the tropopause near 100 mbar to at least 10 bars, in an atmospheric layer of depth beneath the tropopause of about 120 km at Neptune and about 150 km at Uranus.The Jet Propulsion Laboratory’s Quadrupole Ion Trap Mass Spectrometer (QITMS) is being developed to achieve all of these requirements. A compact, wireless instrument with a mass of only 7.5 kg, and a volume of 7 liters (7U), the JPL QITMS is currently the smallest flight mass spectrometer available for possible use on planetary descent probes as well as small bodies, including comet landers and surface sample return missions. The QITMS is capable of making measurements of all required constituents in the mass range of 1–600 atomic mass units (u) at a typical speed of 50 mass spectra per second, with a sensitivity of up to $10^{13}$ 10 13  counts/mbar/sec and mass resolution of $m/\Delta m=18000$ m / Δ m = 18000 at m/q = 40. (Throughout this paper we use the unit of m/q = u/e for the mass-to-charge ratio, where atomic mass unit and elementary charge are $1~\text{u} = 1.66\times 10^{-27}~\text{kg}$ 1 u = 1.66 × 10 − 27 kg and $1\text{e} = 1.6\times 10^{-19}$ 1 e = 1.6 × 10 − 19 C, respectively.) The QITMS features a novel MEMS-based inlet system driven by a piezoelectric actuator that continuously regulates gas flow at inlet pressures of up to 100 bar.In this paper, we present an overview of the QITMS capabilities, including instrument design and characteristics of the inlet system, as well as the most recent results from laboratory measurements in different modes of operation, especially suitable for ice giant atmospheres exploration.

Development of pattern recognition based on the nanosheet-DNA probes and an extendible DNA library

The Analyst ◽  
2021 ◽  
Author(s):  
Jiawei Qi ◽  
Pinhua Rao ◽  
Lele Wang ◽  
Li Xu ◽  
Yanli Wen ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
High Throughput ◽  
Analysis Data ◽  
Dna Probes ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Library ◽  
Array Sensing

Pattern recognition, also called “array sensing” is a recognition strategy with a wide and expandable analysis range, based on the high-throughput analysis data. In this work, we constructed a sensor...

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