Active DLP Hyperspectral Illumination: A Noninvasive, in Vivo, System Characterization Visualizing Tissue Oxygenation at Near Video Rates

2011 ◽  
Vol 83 (19) ◽  
pp. 7424-7430 ◽  
Author(s):  
Karel J. Zuzak ◽  
Robert P. Francis ◽  
Eleanor F. Wehner ◽  
Maritoni Litorja ◽  
Jeffrey A. Cadeddu ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
System Characterization

scholarly journals Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 485
Author(s):  
Veronika Huntosova ◽  
Denis Horvath ◽  
Robert Seliga ◽  
Georges Wagnieres
Keyword(s):  
Oxidative Stress ◽  
Tissue Oxygenation ◽  
Intact Cell ◽  
Molecular Probes ◽  
Stress Factors ◽  
Time Resolved ◽  
Invasive Approach ◽  
Oxygen Detection

Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)3]2+ in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTrackerTM OrangeCMTMRos fluorescence and [Ru(Phen)3]2+ phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)3]2+ was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, H2O2, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation.

Monitoring the Dynamics of Tissue Oxygenation in Vivo by Phosphorescence Quenching

2003 ◽  
pp. 1-5 ◽  
Author(s):  
David F. Wilson ◽  
Sergei A. Vinogradov ◽  
Vladimir Rozhkov ◽  
Jennifer Creed ◽  
Ivo Rietveld ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Phosphorescence Quenching

scholarly journals Wireless, battery-free optoelectronic systems as subdermal implants for local tissue oximetry

2019 ◽  
Vol 5 (3) ◽  
pp. eaaw0873 ◽  
Author(s):  
Hao Zhang ◽  
Philipp Gutruf ◽  
Kathleen Meacham ◽  
Michael C. Montana ◽  
Xingyue Zhao ◽  
...  
Keyword(s):  
Animal Models ◽  
Tissue Oxygenation ◽  
Human Subjects ◽  
Brain Regions ◽  
Disease States ◽  
Data Output ◽  
Subdermal Implants ◽  
Underlying Mechanisms ◽  
Tissue Oximetry

Monitoring regional tissue oxygenation in animal models and potentially in human subjects can yield insights into the underlying mechanisms of local O2-mediated physiological processes and provide diagnostic and therapeutic guidance for relevant disease states. Existing technologies for tissue oxygenation assessments involve some combination of disadvantages in requirements for physical tethers, anesthetics, and special apparatus, often with confounding effects on the natural behaviors of test subjects. This work introduces an entirely wireless and fully implantable platform incorporating (i) microscale optoelectronics for continuous sensing of local hemoglobin dynamics and (ii) advanced designs in continuous, wireless power delivery and data output for tether-free operation. These features support in vivo, highly localized tissue oximetry at sites of interest, including deep brain regions of mice, on untethered, awake animal models. The results create many opportunities for studying various O2-mediated processes in naturally behaving subjects, with implications in biomedical research and clinical practice.

Association of in-vivo and ex-vivo measurements of tissue oxygenation and photosensitizer concentration in patients with intraperitoneal caracinomatosis

2006 ◽  
Author(s):  
H.-W. Wang ◽  
K. Lee ◽  
A. G. Yodh ◽  
J. C. Finlay ◽  
T. C. Zhu ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Ex Vivo

scholarly journals In vivo evidence of methamphetamine induced attenuation of brain tissue oxygenation as measured by EPR oximetry

2014 ◽  
Vol 275 (2) ◽  
pp. 73-78 ◽  
Author(s):  
John Weaver ◽  
Yirong Yang ◽  
Rebecca Purvis ◽  
Theodore Weatherwax ◽  
Gerald M. Rosen ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Tissue Oxygenation ◽  
Brain Tissue Oxygenation ◽  
Epr Oximetry
Sign in / Sign up

Export Citation Format

Share Document