Monitoring the Dynamics of Tissue Oxygenation in Vivo by Phosphorescence Quenching

2003 ◽  
pp. 1-5 ◽  
Author(s):  
David F. Wilson ◽  
Sergei A. Vinogradov ◽  
Vladimir Rozhkov ◽  
Jennifer Creed ◽  
Ivo Rietveld ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Phosphorescence Quenching

scholarly journals POTENTIAL USAGE OF LIPOSOME-ENCAPSULATED PHOSPHOR FOR IN VIVO IMAGING OF TISSUE OXYGENATION

2004 ◽  
Vol 16 (04) ◽  
pp. 224-232 ◽  
Author(s):  
LEU-WEI LO ◽  
JA-AN A. HO ◽  
YEN-HSIUNG CHANG ◽  
CHIA-HUA CHANG ◽  
CHUNG-SHI YANG
Keyword(s):  
Frequency Domain ◽  
Oxygen Concentration ◽  
Tissue Oxygenation ◽  
Water Solubility ◽  
High Water ◽  
Hepatic Tissue ◽  
High Temporal Resolution ◽  
Low Oxygen ◽  
Phosphorescence Quenching

Oxygen-dependent quenching of phosphorescence can provide a quantitative measurement with high temporal resolution of tissue oxygenation in vivo. It is a real-time optical means for prognosis of diseases where the oxygen concentration is essential. Phosphorescence quenching is a noninvasive methodology, otherwise no more than minimally invasive as the phosphor is necessarily introduced into vasculature prior to the measurement. Oxyphor R2, a dendritic phosphor with twolayer of glutamates, is a suitable phosphor for oxygen measurements owing to its high water solubility. We used a frequency-domain, phase modulation based instrument to calibrate Oxyphor R2. The acquired quenching constant (kQ) and lifetime at zero oxygen (τ°) were consistent with those calibrated from conventional time-domain instrument. Administered with Oxygen R2, the rat hepatic oxygen distributions were imaged throughout the course of ischemia and reperfusion. After 5 min ischemia and subsequent 20 min reperfusion, distinct ischemic areas on the hepatic tissue were observed. In order to extend the application of in vivo oxygen imaging using phosphorescence quenching by minimizing the possible immunoresponse induced by phosphor, we are the first to co-synthesize the dipalmitoylphosphatidylglycerol (DPPG)-rich liposome with Oxyphor R2 to generate the liposome-encapsulated Oxyphor R2. Its calibration using the frequency domain measurement displayed higher quenching constant and shorter lifetime at zero oxygen (kQ = 1186 mmHg−1 sec−1; τ° = 150 μsec) comparing to original Oxyphor R2 (kQ = 438 mmHg−1 sec−1; τ° = 630 μsec). It implicated that the liposome-encapsulated Oxyphor R2 designed to neutralize the immunoresponse could be further applied to measure the tissue oxygenation in vivo, especially those with low oxygen concentration such as tumor.

scholarly journals Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 485
Author(s):  
Veronika Huntosova ◽  
Denis Horvath ◽  
Robert Seliga ◽  
Georges Wagnieres
Keyword(s):  
Oxidative Stress ◽  
Tissue Oxygenation ◽  
Intact Cell ◽  
Molecular Probes ◽  
Stress Factors ◽  
Time Resolved ◽  
Invasive Approach ◽  
Oxygen Detection

Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)3]2+ in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTrackerTM OrangeCMTMRos fluorescence and [Ru(Phen)3]2+ phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)3]2+ was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, H2O2, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation.

scholarly journals Effects of aging and exercise training on spinotrapezius muscle microvascular Po2 dynamics and vasomotor control

2011 ◽  
Vol 110 (3) ◽  
pp. 695-704 ◽  
Author(s):  
Danielle J. McCullough ◽  
Robert T. Davis ◽  
James M. Dominguez ◽  
John N. Stabley ◽  
Christian S. Bruells ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Exercise Training ◽  
Sodium Nitroprusside ◽  
Vascular Function ◽  
Treadmill Running ◽  
Aged Rats ◽  
Phosphorescence Quenching

With advancing age, there is a reduction in exercise tolerance, resulting, in part, from a perturbed ability to match O2 delivery to uptake within skeletal muscle. In the spinotrapezius muscle (which is not recruited during incline treadmill running) of aged rats, we tested the hypotheses that exercise training will 1) improve the matching of O2 delivery to O2 uptake, evidenced through improved microvascular Po2 (PmO2), at rest and throughout the contractions transient; and 2) enhance endothelium-dependent vasodilation in first-order arterioles. Young (Y, ∼6 mo) and aged (O, >24 mo) Fischer 344 rats were assigned to control sedentary (YSED; n = 16, and OSED; n = 15) or exercise-trained (YET; n = 14, and OET; n = 13) groups. Spinotrapezius blood flow (via radiolabeled microspheres) was measured at rest and during exercise. Phosphorescence quenching was used to quantify PmO2 in vivo at rest and across the rest-to-twitch contraction (1 Hz, 5 min) transition in the spinotrapezius muscle. In a follow-up study, vasomotor responses to endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) stimuli were investigated in vitro. Blood flow to the spinotrapezius did not increase above resting values during exercise in either young or aged groups. Exercise training increased the precontraction baseline PmO2 (OET 37.5 ± 3.9 vs. OSED 24.7 ± 3.6 Torr, P < 0.05); the end-contracting PmO2 and the time-delay before PmO2 fell in the aged group but did not affect these values in the young. Exercise training improved maximal vasodilation in aged rats to acetylcholine (OET 62 ± 16 vs. OSED 27 ± 16%) and to sodium nitroprusside in both young and aged rats. Endurance training of aged rats enhances the PmO2 in a nonrecruited skeletal muscle and is associated with improved vascular smooth muscle function. These data support the notion that improvements in vascular function with exercise training are not isolated to the recruited muscle.

scholarly journals Wireless, battery-free optoelectronic systems as subdermal implants for local tissue oximetry

2019 ◽  
Vol 5 (3) ◽  
pp. eaaw0873 ◽  
Author(s):  
Hao Zhang ◽  
Philipp Gutruf ◽  
Kathleen Meacham ◽  
Michael C. Montana ◽  
Xingyue Zhao ◽  
...  
Keyword(s):  
Animal Models ◽  
Tissue Oxygenation ◽  
Human Subjects ◽  
Brain Regions ◽  
Disease States ◽  
Data Output ◽  
Subdermal Implants ◽  
Underlying Mechanisms ◽  
Tissue Oximetry

Monitoring regional tissue oxygenation in animal models and potentially in human subjects can yield insights into the underlying mechanisms of local O2-mediated physiological processes and provide diagnostic and therapeutic guidance for relevant disease states. Existing technologies for tissue oxygenation assessments involve some combination of disadvantages in requirements for physical tethers, anesthetics, and special apparatus, often with confounding effects on the natural behaviors of test subjects. This work introduces an entirely wireless and fully implantable platform incorporating (i) microscale optoelectronics for continuous sensing of local hemoglobin dynamics and (ii) advanced designs in continuous, wireless power delivery and data output for tether-free operation. These features support in vivo, highly localized tissue oximetry at sites of interest, including deep brain regions of mice, on untethered, awake animal models. The results create many opportunities for studying various O2-mediated processes in naturally behaving subjects, with implications in biomedical research and clinical practice.

Association of in-vivo and ex-vivo measurements of tissue oxygenation and photosensitizer concentration in patients with intraperitoneal caracinomatosis

2006 ◽  
Author(s):  
H.-W. Wang ◽  
K. Lee ◽  
A. G. Yodh ◽  
J. C. Finlay ◽  
T. C. Zhu ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Ex Vivo

Active DLP Hyperspectral Illumination: A Noninvasive, in Vivo, System Characterization Visualizing Tissue Oxygenation at Near Video Rates

2011 ◽  
Vol 83 (19) ◽  
pp. 7424-7430 ◽  
Author(s):  
Karel J. Zuzak ◽  
Robert P. Francis ◽  
Eleanor F. Wehner ◽  
Maritoni Litorja ◽  
Jeffrey A. Cadeddu ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
System Characterization
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