Determination of Unsaturation Distribution in Polybutadienes by Infrared Spectrometry

1959 ◽  
Vol 31 (4) ◽  
pp. 529-532 ◽  
Author(s):  
R. S. Silas ◽  
Joy. Yates ◽  
Vernon. Thornton

1962 ◽  
Vol 41 (2) ◽  
pp. 234-246 ◽  
Author(s):  
H. J. van der Molen

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.



The Analyst ◽  
1999 ◽  
Vol 124 (3) ◽  
pp. 367-371 ◽  
Author(s):  
Kazem Kargosha ◽  
Seyyed Hamid Ahmadi ◽  
Alireza Ghassempour ◽  
Mohammad Reza Arshadi




Talanta ◽  
2009 ◽  
Vol 78 (2) ◽  
pp. 584-589 ◽  
Author(s):  
Mohsen Zeeb ◽  
Mohammad Reza Ganjali ◽  
Parviz Norouzi ◽  
Seyyed Rezvan Moeinossadat






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