scholarly journals Kinetic Size-Exclusion Chromatography with Mass Spectrometry Detection: An Approach for Solution-Based Label-Free Kinetic Analysis of Protein–Small Molecule Interactions

2014 ◽  
Vol 86 (20) ◽  
pp. 10016-10020 ◽  
Author(s):  
Jiayin Bao ◽  
Svetlana M. Krylova ◽  
Leonid T. Cherney ◽  
J. C. Yves LeBlanc ◽  
Patrick Pribil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Kinetic Analysis ◽  
Size Exclusion Chromatography ◽  
Small Molecule ◽  
Size Exclusion ◽  
Label Free ◽  
Mass Spectrometry Detection ◽  
Exclusion Chromatography ◽  
Molecule Interactions

Pre-equilibration kinetic size-exclusion chromatography with mass spectrometry detection (peKSEC-MS) for label-free solution-based kinetic analysis of protein–small molecule interactions

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 990-994 ◽  
Author(s):  
Jiayin Bao ◽  
Svetlana M. Krylova ◽  
Leonid T. Cherney ◽  
J. C. Yves Le Blanc ◽  
Patrick Pribil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Kinetic Analysis ◽  
Small Molecule ◽  
Size Exclusion ◽  
Free Solution ◽  
Label Free ◽  
Mass Spectrometry Detection ◽  
Reversible Binding ◽  
Exclusion Chromatography ◽  
Molecule Interactions

Label-free solution-based kinetic analysis of reversible binding between protein and small molecule.

Untargeted Defining Protein–Metabolites Interaction Based on Label-Free Kinetic Size Exclusion Chromatography-Mass Spectrometry

2020 ◽  
Vol 92 (11) ◽  
pp. 7657-7665
Author(s):  
Bohong Wang ◽  
Wangjie Lv ◽  
Mengmeng Chang ◽  
Chunxia Zhao ◽  
Xianzhe Shi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Label Free ◽  
Chromatography Mass Spectrometry ◽  
Exclusion Chromatography

scholarly journals Mass-Spectrometry Based Proteome Comparison of Extracellular Vesicle Isolation Methods: Comparison of ME-kit, Size-Exclusion Chromatography, and High-Speed Centrifugation

Biomedicines ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 246 ◽  
Author(s):  
Anders Askeland ◽  
Anne Borup ◽  
Ole Østergaard ◽  
Jesper V. Olsen ◽  
Sigrid M. Lund ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
High Speed ◽  
Proteome Analysis ◽  
Extracellular Vesicle ◽  
Size Exclusion ◽  
Label Free ◽  
Protein Biomarkers ◽  
Transmission Electron ◽  
Exclusion Chromatography

Extracellular vesicles (EVs) are small membrane-enclosed particles released by cells under various conditions specific to cells’ biological states. Hence, mass-spectrometry (MS) based proteome analysis of EVs in plasma has gained much attention as a method to discover novel protein biomarkers. MS analysis of EVs in plasma is challenging and EV isolation is usually necessary. Therefore, we compared differences in abundance, subtypes, and contamination for EVs isolated by high-speed centrifugation, size exclusion chromatography (SEC), and peptide-affinity precipitation (PAP/ME kit) for subsequent MS-based proteome analysis. Successful EV isolation was evaluated by nanoparticle-tracking analysis, immunoblotting, and transmission electron microscopy, while EV abundance, EV subtypes, and contamination was evaluated by label-free tandem MS. High-speed centrifugation and SEC isolates showed high EV abundance at the expense of contamination by non-EV proteins and lipoproteins, respectively. These two methods also resulted in EVs of a similar type, however, with smaller EVs in SEC isolates. PAP isolates had a relatively low EV abundance and high contamination. We consider high-speed centrifugation and SEC suitable as EV isolation for MS biomarker studies, where the choice between the two should depend on the scientific questions and whether the focus is on larger or smaller EVs or a combination of both.

Kinetic Capillary Electrophoresis with Mass-Spectrometry Detection (KCE-MS) Facilitates Label-Free Solution-Based Kinetic Analysis of Protein-Small Molecule Binding

ChemBioChem ◽  
2011 ◽  
Vol 12 (17) ◽  
pp. 2551-2554 ◽  
Author(s):  
Jiayin Bao ◽  
Svetlana M. Krylova ◽  
Derek J. Wilson ◽  
Oren Reinstein ◽  
Philip E. Johnson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Kinetic Analysis ◽  
Small Molecule ◽  
Free Solution ◽  
Label Free ◽  
Mass Spectrometry Detection ◽  
Kinetic Capillary Electrophoresis
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