Determination of double bond position in monounsaturated fatty acid esters by mass spectrometry of their trimethylsilyloxy derivatives

1968 ◽  
Vol 40 (10) ◽  
pp. 1458-1463 ◽  
Author(s):  
Pompeo. Capella ◽  
C. M. Zorzut
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Double Bond ◽  
Double Bond Position ◽  
Fatty Acid Esters ◽  
Monounsaturated Fatty Acid ◽  
Acid Esters

Simultaneous determination of 3-MCPD fatty acid esters and glycidol fatty acid esters in edible oils using liquid chromatography time-of-flight mass spectrometry

LWT ◽  
2012 ◽  
Vol 48 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Katsuhito Hori ◽  
Natsuko Koriyama ◽  
Hitomi Omori ◽  
Masamitsu Kuriyama ◽  
Toshiharu Arishima ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Simultaneous Determination ◽  
Edible Oils ◽  
Time Of Flight ◽  
Fatty Acid Esters ◽  
Flight Mass Spectrometry ◽  
Acid Esters

2-Monochloropropanediol (2-MCPD), 3-Monochloropropanediol (3-MCPD), and Glycidol in Infant and Adult/Pediatric Nutritional Formula: Single-Laboratory Validation, First Action 2018.12

2019 ◽  
Vol 102 (4) ◽  
pp. 1205-1220 ◽  
Author(s):  
Jan Kuhlmann
Keyword(s):  
Fatty Acid ◽  
Infant Formula ◽  
Edible Oils ◽  
Accurate Determination ◽  
Fatty Acid Esters ◽  
Method Performance ◽  
Relative Standard ◽  
Single Laboratory ◽  
Acid Esters

Abstract Background: Fatty acid esters of glycidol, 2-Monochloropropanediol (MCPD), and 3-MCPD are heat-induced foodborne processing contaminants with possible adverse health effects. These compounds occur frequently in refined edible oils. Consequently, glycidyl esters and 2- and 3-MCPD esters might also be present in foods that contain refined edible oils. Objective: This manuscript describes the single-laboratory validation of an analytical method for the quantitative determination of glycidol, 2-MCPD, and 3-MCPD present as fatty acid esters or as free 2- or 3-MCPD in infant and adult/pediatric nutritional formula. Methods: Technically, the presented method is based on the combination of a Heat-Ultrasound Pressure-supported Solvent Extraction and a GC–MS determination of glycidol, 2-MCPD, and 3-MCPD. From a chemical perspective, the method includes an alkaline catalyzed transesterification, conversion of the unstable glycidol into monobromopropanediol, and the parallel derivatization of all analytes with phenylboronic acid. Results: Validation results showed that method linearity for all analytes in powdered and liquid infant formula ranged from 0.9981 to 0.9999 (n = 18). Repeatability relative standard deviation values for concentration levels between 1.3 μg/kg and 331 μg/kg were in the range of 1 to 12%. Relative recoveries were found to be between 93 and 107%. The analytes were quantifiable down to 5–10 μg/kg in powdered samples and 1–2 μg/kg in liquid samples. Conclusions: The reported results met actual AOAC Standard Method Performance Requirements. Highlights: In terms of consumer protection, the presented method is a novel approach for the sensitive and accurate determination of glycidol, 2-MCPD, and 3-MCPD in infant formula and related foodstuffs.

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